Sudarshan Rajagopal, Jason M. Key, Erin B. Purcell, David J. Boerema, Keith Moffat
The Escherichia coli protein YcgF contains a photosensory flavin adenine dinucleotide (FAD)–binding BLUF domain covalently linked to an EAL domain, which is predicted to have cyclic-di-guanosine...
Spencer Anderson, Vukica Šrajer, Keith Moffat
We investigate by X-ray crystallographic techniques the cryotrapped states that accumulate on controlled illumination of the blue light photoreceptor, photoactive yellow protein (PYP), at 110 K in...
Batterman, Boris W., Bilderback, Donald, Mills, Dennis M., Bedzyk, Michael J., Volz, Karl W., Schildkamp, Wilfried, ...
IN THIS ISSUE: Making Good Use of Synchrotron Radiation: The Role of CHESS at Cornell and as a National Facility /3 Boris W. Batterman ... X-Ray Optics: A Vital Aspect of Work with Synchrotron...
Batterman, Boris W., Bilderback, Donald, Mills, Dennis M., Bedzyk, Michael J., Volz, Karl W., Schildkamp, Wilfried, ...
IN THIS ISSUE: Making Good Use of Synchrotron Radiation: The Role of CHESS at Cornell and as a National Facility /3 Boris W. Batterman ... X-Ray Optics: A Vital Aspect of Work with Synchrotron...
Phototropin, a major blue-light receptor for phototropism in seed plants, exhibits blue-light-dependent autophosphorylation and contains two light, oxygen, or voltage (LOV) domains and a...
Rajagopal, Sudarshan, Moffat, Keith
Photoactive yellow protein (E-PYP) is a blue light photoreceptor, implicated in a negative phototactic response in Ectothiorhodospira halophila, that also serves as a model for the Per–Arnt–Sim...
Photoexcited Structure of a Plant Photoreceptor Domain Reveals a Light-Driven Molecular Switch
The phototropins are flavoprotein kinases that control phototropic bending, light-induced chloroplast movement, and stomatal opening in plants. Two flavin mononucleotide binding light, oxygen, or...
Protein kinetics: Structures of intermediates and reaction mechanism from time-resolved x-ray data
Schmidt, Marius, Pahl, Reinhard, Srajer, Vukica, Anderson, Spencer, Ren, Zhong, Ihee, Hyotcherl, ...
We determine the number of authentic reaction intermediates in the later stages of the photocycle of photoactive yellow protein at room temperature, their atomic structures, and a consistent set of...
Baxter, Richard H. G., Ponomarenko, Nina, Šrajer, Vukica, Pahl, Reinhard, Moffat, Keith, Norris, James R.
Light-induced structural changes in the bacterial reaction center were studied by a time-resolved crystallographic experiment. Crystals of protein from Blastochloris viridis (formerly...
Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds
Ihee, Hyotcherl, Rajagopal, Sudarshan, Šrajer, Vukica, Pahl, Reinhard, Anderson, Spencer, Schmidt, Marius, ...
Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use time-resolved...
Schmidt, Marius, Rajagopal, Sudarshan, Ren, Zhong, Moffat, Keith
Singular value decomposition (SVD) is a technique commonly used in the analysis of spectroscopic data that both acts as a noise filter and reduces the dimensionality of subsequent least-squares fits....
Phototropin, a major blue-light receptor for phototropism in seed plants, exhibits blue-light-dependent autophosphorylation and contains two light, oxygen, or voltage (LOV) domains and a...
Rajagopal, Sudarshan, Moffat, Keith
Photoactive yellow protein (E-PYP) is a blue light photoreceptor, implicated in a negative phototactic response in Ectothiorhodospira halophila, that also serves as a model for the Per–Arnt–Sim...
Photoexcited Structure of a Plant Photoreceptor Domain Reveals a Light-Driven Molecular Switch
The phototropins are flavoprotein kinases that control phototropic bending, light-induced chloroplast movement, and stomatal opening in plants. Two flavin mononucleotide binding light, oxygen, or...
Protein kinetics: Structures of intermediates and reaction mechanism from time-resolved x-ray data
Schmidt, Marius, Pahl, Reinhard, Srajer, Vukica, Anderson, Spencer, Ren, Zhong, Ihee, Hyotcherl, ...
We determine the number of authentic reaction intermediates in the later stages of the photocycle of photoactive yellow protein at room temperature, their atomic structures, and a consistent set of...
Baxter, Richard H. G., Ponomarenko, Nina, Šrajer, Vukica, Pahl, Reinhard, Moffat, Keith, Norris, James R.
Light-induced structural changes in the bacterial reaction center were studied by a time-resolved crystallographic experiment. Crystals of protein from Blastochloris viridis (formerly...
Visualizing reaction pathways in photoactive yellow protein from nanoseconds to seconds
Ihee, Hyotcherl, Rajagopal, Sudarshan, Šrajer, Vukica, Pahl, Reinhard, Anderson, Spencer, Schmidt, Marius, ...
Determining 3D intermediate structures during the biological action of proteins in real time under ambient conditions is essential for understanding how proteins function. Here we use time-resolved...
Schmidt, Marius, Rajagopal, Sudarshan, Ren, Zhong, Moffat, Keith
Singular value decomposition (SVD) is a technique commonly used in the analysis of spectroscopic data that both acts as a noise filter and reduces the dimensionality of subsequent least-squares fits....
Yeremenko, Sergey, Moffat, Keith, Hellingwerf, Klaas J.
Time-resolved ultraviolet-visible spectroscopy was used to characterize the photocycle transitions in single crystals of wild-type and the E-46Q mutant of photoactive yellow protein (PYP) with...
Yang, Xiaojing, Stojković, Emina A., Kuk, Jane, Moffat, Keith
Bacteriophytochromes RpBphP2 and RpBphP3 from the photosynthetic bacterium Rhodopseudomonas palustris work in tandem to modulate synthesis of the light-harvesting complex LH4 in response to light....
Light-activated DNA binding in a designed allosteric protein
Strickland, Devin, Moffat, Keith, Sosnick, Tobin R.
An understanding of how allostery, the conformational coupling of distant functional sites, arises in highly evolvable systems is of considerable interest in areas ranging from cell biology to...
Yang, Xiaojing, Kuk, Jane, Moffat, Keith
Phytochromes are red-light photoreceptors that regulate light responses in plants, fungi, and bacteria via reversible photoconversion between red (Pr) and far-red (Pfr) light-absorbing states. Here...