Ken-ichi Honjoh

Influence of Cold Hardening on Chlorophyll and Carotenoid in Chlorella vulgaris (2009)

Watanabe, Yuta, Yamada, Naotaka, Machida, Takeshi, Honjoh, Ken-ichi, Kuwano, Eiichi, 渡邉, 雄太, ...

Chlorella vulgaris C-27 has developed freezing tolerance by hardening at 3℃ for 24 hour. It was reported that many genes which encode proteins related stress response, storage, protein synthesis...

Molecular characterization of low-temperature-inducible NTR-C in Chlorella vulgaris (2007)

Machida, Takeshi, Kato, Eri, Ishibashi, Akiko, Ohashi, Naoto, Honjoh, Ken-ichi, Miyamoto, Takahisa

We isolated a cDNA corresponding to a chloroplast NADPH-dependent thioredoxin reductase gene (NTR-C), in Chlorella that is low-temperature-inducible. The obtained cDNA was 1,838 bp in length and...

Freezing Tolerance of Transgenic Tobacco with Increased Content of Unsaturated Fatty Acid by Expressiong the CvFad3 Gene (2003)

Shimizu, Hideyuki, Furuya, Naoki, Suga, Koushirou, Honjoh, Ken-ichi, Miyamoto, Takahisa, Hatano, Shoji, ...

Two fatty acid desaturases were independently expressed in tobacco plants and freezing torelance of the transgenic plants was investigated: the two disaturases were CvFAD2, which desaturases...

Preparation of Protoplasts from Chlorella vulgaris K-73122 and Cell Wall Regeneration of Protoplasts from C. vulgaris K-73122 and C-27 (2003)

Honjoh, Ken-ichi, Suga, Koushirou, Shinohara, Fuminori, Maruyama, Isao, Miyamoto, Takahisa, Hatano, Shoji, ...

Protoplasts from Chlorella vulgaris K-73112 were obtained by enzymatic digestion with a mixture of Acromopeptidase, Cellulase ONOZUKA R-10, Chitosanas Kl, Gluczyme, and Uskizyme. The formation of...

Molecular Cloning and Expression of Hardening-Induced Genes in Chlorella vulgaris C-27: the Most Abundant Clone Encodes a Late Embryogenesis Abundant Protein (1995)

Joh, Toshio, Honjoh, Ken-ichi, Yoshimoto, Makoto, Funabashi, Jun, Miyamoto, Takahisa, Hatano, Shoji

To investigate the effects of hardening on gene expression in Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27), a frost-hardy strain, 17 cDNA clones...

Isolation and Characterization of Hardening-Induced Proteins in Chlorella vulgaris C-27: Identification of Late Embryogenesis Abundant Proteins (1995)

Honjoh, Ken-ichi, Yoshimoto, Makoto, Joh, Toshio, Kajiwara, Taishin, Miyamoto, Takahisa, Hatano, Shoji

Hardening-induced soluble proteins of Chlorella vulgaris Beijerink IAM C-27 (formerly Chlorella ellipsoidea Gerneck IAM C-27) were isolated and purified by two-dimensional high-performance liquid...