Ken-ichi Nishiyama

Publication List Details

Period

1996 - 2002

Number

9

Co-Authors

Membrane Topology Inversion of SecG Detected by Labeling with a Membrane-Impermeable Sulfhydryl Reagent that Causes a Close Association of SecG with SecA (2002)

Nagamori, Shushi, Nishiyama, Ken-ichi, Tokuda, Hajime

SecG stimulates protein translocation in Escherichia coli by facilitating the membrane insertion-deinsertion cycle of SecA. SecG was previously shown to undergo membrane topology inversion, since...

Two SecG Molecules Present in a Single Protein Translocation Machinery Are Functional Even after Crosslinking (2000)

Nagamori, Shushi, Nishiyama, Ken-ichi, Tokuda, Hajime

SecG, a membrane component of the protein translocation apparatus of Escherichia coli, undergoes membrane topology inversion, which is coupled to the membrane insertion and deinsertion cycle of SecA....

Roles of the Conserved Cytoplasmic Region and Non-Conserved Carboxy-Terminal Region of SecE in Escherichia coli Protein Translocase (1996)

Kontinen, Vesa P., Yamanaka, Miyuki, Nishiyama, Ken-ichi, Tokuda, Hajime

SecE, an essential membrane component of the Escherichia coli protein translocase, consists of 127 ami no acid residues. Only a part of the second putative cytoplasmic region comprising some 13...

Overexpression of yccL (gnsA) and ydfY (gnsB) Increases Levels of Unsaturated Fatty Acids and Suppresses both the Temperature-Sensitive fabA6 Mutation and Cold-Sensitive secG Null Mutation of Escherichia coli

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

A multicopy suppressor of the cold-sensitive secG null mutation was isolated. The suppressor contained sfa and yccL, the former of which has been reported to be a multicopy suppressor of the fabA6...

Overexpression of gnsA, a Multicopy Suppressor of the secG Null Mutation, Increases Acidic Phospholipid Contents by Inhibiting Phosphatidylethanolamine Synthesis at Low Temperatures

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

GnsA overproduction was previously found to suppress both the secG null mutation and the fabA6 mutation in Escherichia coli by increasing the unsaturated fatty acid contents. We report here that it...

Overexpression of yccL (gnsA) and ydfY (gnsB) Increases Levels of Unsaturated Fatty Acids and Suppresses both the Temperature-Sensitive fabA6 Mutation and Cold-Sensitive secG Null Mutation of Escherichia coli

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

A multicopy suppressor of the cold-sensitive secG null mutation was isolated. The suppressor contained sfa and yccL, the former of which has been reported to be a multicopy suppressor of the fabA6...

Overexpression of gnsA, a Multicopy Suppressor of the secG Null Mutation, Increases Acidic Phospholipid Contents by Inhibiting Phosphatidylethanolamine Synthesis at Low Temperatures

Sugai, Rie, Shimizu, Hisayo, Nishiyama, Ken-ichi, Tokuda, Hajime

GnsA overproduction was previously found to suppress both the secG null mutation and the fabA6 mutation in Escherichia coli by increasing the unsaturated fatty acid contents. We report here that it...