Cirimotich, Chris M, Scott, Jaclyn C, Phillips, Aaron T, Geiss, Brian J, Olson, Ken E
Abstract Background Arthropod-borne viruses (arboviruses) can persistently infect and cause limited damage to mosquito vectors. RNA interference (RNAi) is a mosquito antiviral response important in...
Bmc Microbiology, Christopher M Cirimotich, Jaclyn C Scott, Aaron T Phillips, Brian J Geiss, Ken E Olson, ...
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Aedes aegyptiuses RNA interference in defense against Sindbis virus infection (2008)
Campbell, Corey L, Keene, Kimberly M, Brackney, Douglas E, Olson, Ken E, Blair, Carol D, Wilusz, Jeffrey, ...
Abstract Background RNA interference (RNAi) is an important anti-viral defense mechanism. The Aedes aegypti genome encodes RNAi component orthologs, however, most populations of this mosquito are...
Recombination-ready Sindbis replicon expression vectors for transgene expression (2007)
Geiss, Brian J, Shimonkevitz, Lisa H, Sackal, Cherilyn I, Olson, Ken E
Abstract Background Sindbis viruses have been widely used as tools to study gene function in cells. Despite the utility of these systems, the construction and production of alphavirus replicons is...
Dengue virus type 2: replication and tropisms in orally infected Aedes aegyptimosquitoes (2007)
Salazar, Ma Isabel, Richardson, Jason H, Sánchez-Vargas, Irma, Olson, Ken E, Beaty, Barry J
Abstract Background To be transmitted by its mosquito vector, dengue virus (DENV) must infect midgut epithelial cells, replicate and disseminate into the hemocoel, and finally infect the salivary...
Kevin M. Myles, Dennis J. Pierro, Ken E. Olson
Within mosquitoes, arboviruses encounter barriers to infection and dissemination that are critical determinants of vector competence. The molecular mechanisms responsible for these barriers have yet...
Johnson, Barbara W., Olson, Ken E., Allen-Miura, Tanya, Rayms-Keller, Alfredo, Carlson, Jonathan O., Coates, Craig J., ...
A rapid and reproducible method of inhibiting the expression of specific genes in mosquitoes should further our understanding of gene function and may lead to the identification of mosquito genes...
Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes
Blair, Carol D., Adelman, Zachary N., Olson, Ken E.
Arthropod-borne virus (arbovirus) infections cause a number of emerging and resurgent human and veterinary infectious diseases. Traditional means of controlling arbovirus diseases include vaccination...
Expression of Human MxA Protein in Mosquito Cells Interferes with LaCrosse Virus Replication
Miura, Tanya A., Carlson, Jonathan O., Beaty, Barry J., Bowen, Richard A., Olson, Ken E.
Human MxA protein inhibits LaCrosse virus (LAC virus; family Bunyaviridae) replication in vertebrate cells and MxA-transgenic mice. LAC virus is transmitted to humans by Aedes triseriatus mosquitoes....
Adelman, Zach N., Sanchez-Vargas, Irma, Travanty, Emily A., Carlson, Jon O., Beaty, Barry J., Blair, Carol D., ...
Double-stranded RNA (dsRNA) initiates cellular posttranscriptional responses that are collectively called RNA silencing in a number of different organisms, including plants, nematodes, and fruit...
Myles, Kevin M., Pierro, Dennis J., Olson, Ken E.
The Sindbis virus (Alphavirus; Togaviridae) strain MRE16 efficiently infects Aedes aegypti mosquitoes that ingest a blood meal containing 8 to 9 log10 PFU of virus/ml. However, a small-plaque variant...
Uhlirova, Mirka, Foy, Brian D., Beaty, Barry J., Olson, Ken E., Riddiford, Lynn M., Jindra, Marek
The transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly...
Keene, Kimberly M., Foy, Brian D., Sanchez-Vargas, Irma, Beaty, Barry J., Blair, Carol D., Olson, Ken E.
RNA interference (RNAi) is triggered in eukaryotic organisms by double-stranded RNA (dsRNA), and it destroys any mRNA that has sequence identity with the dsRNA trigger. The RNAi pathway in Anopheles...
Franz, Alexander W. E., Sanchez-Vargas, Irma, Adelman, Zach N., Blair, Carol D., Beaty, Barry J., James, Anthony A., ...
Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in...
Johnson, Barbara W., Olson, Ken E., Allen-Miura, Tanya, Rayms-Keller, Alfredo, Carlson, Jonathan O., Coates, Craig J., ...
A rapid and reproducible method of inhibiting the expression of specific genes in mosquitoes should further our understanding of gene function and may lead to the identification of mosquito genes...
Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes
Blair, Carol D., Adelman, Zachary N., Olson, Ken E.
Arthropod-borne virus (arbovirus) infections cause a number of emerging and resurgent human and veterinary infectious diseases. Traditional means of controlling arbovirus diseases include vaccination...
Expression of Human MxA Protein in Mosquito Cells Interferes with LaCrosse Virus Replication
Miura, Tanya A., Carlson, Jonathan O., Beaty, Barry J., Bowen, Richard A., Olson, Ken E.
Human MxA protein inhibits LaCrosse virus (LAC virus; family Bunyaviridae) replication in vertebrate cells and MxA-transgenic mice. LAC virus is transmitted to humans by Aedes triseriatus mosquitoes....
Adelman, Zach N., Sanchez-Vargas, Irma, Travanty, Emily A., Carlson, Jon O., Beaty, Barry J., Blair, Carol D., ...
Double-stranded RNA (dsRNA) initiates cellular posttranscriptional responses that are collectively called RNA silencing in a number of different organisms, including plants, nematodes, and fruit...
Myles, Kevin M., Pierro, Dennis J., Olson, Ken E.
The Sindbis virus (Alphavirus; Togaviridae) strain MRE16 efficiently infects Aedes aegypti mosquitoes that ingest a blood meal containing 8 to 9 log10 PFU of virus/ml. However, a small-plaque variant...
Uhlirova, Mirka, Foy, Brian D., Beaty, Barry J., Olson, Ken E., Riddiford, Lynn M., Jindra, Marek
The transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly...
Keene, Kimberly M., Foy, Brian D., Sanchez-Vargas, Irma, Beaty, Barry J., Blair, Carol D., Olson, Ken E.
RNA interference (RNAi) is triggered in eukaryotic organisms by double-stranded RNA (dsRNA), and it destroys any mRNA that has sequence identity with the dsRNA trigger. The RNAi pathway in Anopheles...
Franz, Alexander W. E., Sanchez-Vargas, Irma, Adelman, Zach N., Blair, Carol D., Beaty, Barry J., James, Anthony A., ...
Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in...
Dengue virus type 2: replication and tropisms in orally infected Aedes aegypti mosquitoes
Salazar, Ma Isabel, Richardson, Jason H, Sánchez-Vargas, Irma, Olson, Ken E, Beaty, Barry J
Blakqori, Gjon, Delhaye, Sophie, Habjan, Matthias, Blair, Carol D., Sánchez-Vargas, Irma, Olson, Ken E., ...
La Crosse virus (LACV) is a mosquito-transmitted member of the Bunyaviridae family that causes severe encephalitis in children. For the LACV nonstructural protein NSs, previous overexpression studies...
Recombination-ready Sindbis replicon expression vectors for transgene expression
Geiss, Brian J, Shimonkevitz, Lisa H, Sackal, Cherilyn I, Olson, Ken E
Aedes aegypti uses RNA interference in defense against Sindbis virus infection
Campbell, Corey L, Keene, Kimberly M, Brackney, Douglas E, Olson, Ken E, Blair, Carol D, Wilusz, Jeffrey, ...
Pierro, Dennis J., Powers, Erik L., Olson, Ken E.
Wild-type Sindbis virus (SINV) strain MRE16 efficiently infects Aedes aegypti midgut epithelial cells (MEC), but laboratory-derived neurovirulent SINV strain TE/5′2J infects MEC poorly. SINV...
Sánchez-Vargas, Irma, Scott, Jaclyn C., Poole-Smith, B. Katherine, Franz, Alexander W. E., Barbosa-Solomieu, Valérie, Wilusz, Jeffrey, ...
A number of studies have shown that both innate and adaptive immune defense mechanisms greatly influence the course of human dengue virus (DENV) infections, but little is known about the innate...
Lozano-Fuentes, Saul, Fernandez-Salas, Ildefonso, De Lourdes Munoz, Maria, Garcia-Rejon, Julian, Olson, Ken E., Beaty, Barry J., ...
The Neovolcanic axis (NVA) traverses Mexico at the 19th parallel and is considered to be a geographic barrier to many species. We have demonstrated that the intersection of the NVA with the coast in...