Murphy, Kenan C., Ritchie, Jennifer M., Waldor, Matthew K., Lobner-Olesen, Anders, Marinus, Martin G.
Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production,...
Campellone, Kenneth Geno, Roe, Andrew J., Lobner-Olesen, Anders, Murphy, Kenan C., Magoun, Loranne, Brady, Michael J., ...
Enterohaemorrhagic Escherichia coli (EHEC) are highly infectious pathogens capable of causing severe diarrhoeal illnesses. As a critical step during their colonization, EHEC adhere intimately to...
Murphy, Kenan C., Campellone, Kenneth Geno
BACKGROUND: The lambda Red recombineering technology has been used extensively in Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion mutants, but less so in...
Murphy, Kenan C, Campellone, Kenneth G
Abstract Background The λ Red recombineering technology has been used extensively in Escherichia coli and Salmonella typhimurium for easy PCR-mediated generation of deletion mutants, but less so in...
Mutational analysis of the MutH protein from Escherichia coli (2001)
Loh, Tamalette, Murphy, Kenan C., Marinus, Martin G.
Site-directed mutagenesis was performed on several areas of MutH based on the similarity of MutH and PvuII structural models. The aims were to identify DNA-binding residues; to determine whether MutH...
Mutational analysis of the MutH protein from Escherichia coli (2001)
Loh, Tamalette, Murphy, Kenan C., Marinus, Martin G.
Site-directed mutagenesis was performed on several areas of MutH based on the similarity of MutH and PvuII structural models. The aims were to identify DNA-binding residues; to determine whether MutH...
Roles of RuvC and RecG in phage lambda red-mediated recombination (1999)
Poteete, Anthony R., Fenton, Anita C., Murphy, Kenan C.
The recombination properties of Escherichia coli strains expressing the red genes of bacteriophage lambda and lacking recBCD function either by mutation or by expression of lambda gam were examined....
Replacement of Escherichia coli's RecBCD function with phage lambda's Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate...
Biochemical characterization of P22 phage-modified Escherichia coli RecBCD enzyme (1994)
The biochemical properties of phage P22 Abc-modified RecBCD enzyme from Escherichia coli have been examined. RecBCD purified from a cell that expresses Abc (anti-RecBCD) contains all three RecBCD...
Murphy, Kenan C., Lewis, Laura J.
Escherichia coli strains bearing plasmids expressing phage P22 anti-RecBCD functions abc1 and abc2 were tested for the presence of recBC-like phenotypes. Abc2 induces moderate sensitivity to UV light...
The lambda Gam protein was isolated from cells containing a Gam-producing plasmid. The purified Gam protein was found to bind to RecBCD without displacing any of its subunits. Gam was shown to...
Poteete, Anthony R., Fenton, Anita C., Murphy, Kenan C.
Plasmids that express the bacteriophage lambda gam gene or the P22 abc2 gene (with and without abc1) at controllable levels were placed in Escherichia coli and tested for effects on the activity of...
Roles of RuvC and RecG in Phage λ Red-Mediated Recombination
Poteete, Anthony R., Fenton, Anita C., Murphy, Kenan C.
The recombination properties of Escherichia coli strains expressing the red genes of bacteriophage λ and lacking recBCD function either by mutation or by expression of λ gam were examined. The...
Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli
Replacement of Escherichia coli’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate...
Roles of RuvC and RecG in Phage λ Red-Mediated Recombination
Poteete, Anthony R., Fenton, Anita C., Murphy, Kenan C.
The recombination properties of Escherichia coli strains expressing the red genes of bacteriophage λ and lacking recBCD function either by mutation or by expression of λ gam were examined. The...
Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli
Replacement of Escherichia coli’s RecBCD function with phage λ’s Red function generates a strain whose chromosome recombines with short linear DNA fragments at a greatly elevated rate. The rate...
Murphy, Kenan C., Ritchie, Jennifer M., Waldor, Matthew K., Løbner-Olesen, Anders, Marinus, M. G.
Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production,...