Kenichi Nishioka

Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading (2007)

Nakayama, Takahiro, Nishioka, Kenichi, Dong, Yi-Xin, Shimojima, Tsukasa, Hirose, Susumu

Epigenetic maintenance of the expression state of the genome is critical for development. Drosophila GAGA factor interacts with FACT and modulates chromatin structure for the maintenance of gene...

Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes (2002)

Rice, Judd C., Nishioka, Kenichi, Sarma, Kavitha, Steward, Ruth, Reinberg, Danny, Allis, C. David

Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein (2002)

Kuzmichev, Andrei, Nishioka, Kenichi, Erdjument-Bromage, Hediye, Tempst, Paul, Reinberg, Danny

Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation (2002)

Nishioka, Kenichi, Chuikov, Sergei, Sarma, Kavitha, Erdjument-Bromage, Hediye, Allis, C. David, Tempst, Paul, ...

Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen (2001)

Tsuchimoto, Daisuke, Sakai, Yasunari, Sakumi, Kunihiko, Nishioka, Kenichi, Sasaki, Masafumi, Fujiwara, Toshiyuki, ...

In human cells APE1 is the major AP endonuclease and it has been reported to have no functional mitochondrial targeting sequence (MTS). We found that APE2 protein possesses a putative MTS. When its...

Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria (2000)

Ohtsubo, Toshio, Nishioka, Kenichi, Imaiso, Yasuyuki, Iwai, Shigenori, Shimokawa, Hidetoshi, Oda, Hisanobu, ...

An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with...

Expression and Differential Intracellular Localization of Two Major Forms of Human 8-Oxoguanine DNA Glycosylase Encoded by Alternatively Spliced OGG1 mRNAs

Nishioka, Kenichi, Ohtsubo, Toshio, Oda, Hisanobu, Fujiwara, Toshiyuki, Kang, Dongchon, Sugimachi, Keizo, ...

We identified seven alternatively spliced forms of human 8-oxoguanine DNA glycosylase (OGG1) mRNAs, classified into two types based on their last exons (type 1 with exon 7: 1a and 1b; type 2 with...

Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen

Tsuchimoto, Daisuke, Sakai, Yasunari, Sakumi, Kunihiko, Nishioka, Kenichi, Sasaki, Masafumi, Fujiwara, Toshiyuki, ...

In human cells APE1 is the major AP endonuclease and it has been reported to have no functional mitochondrial targeting sequence (MTS). We found that APE2 protein possesses a putative MTS. When its...

Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria

Ohtsubo, Toshio, Nishioka, Kenichi, Imaiso, Yasuyuki, Iwai, Shigenori, Shimokawa, Hidetoshi, Oda, Hisanobu, ...

An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with...

Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes

Rice, Judd C., Nishioka, Kenichi, Sarma, Kavitha, Steward, Ruth, Reinberg, Danny, Allis, C. David

We describe distinct patterns of histone methylation during human cell cycle progression. Histone H4 methyltransferase activity was found to be cell cycle-regulated, consistent with increased H4 Lys...

Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein

Kuzmichev, Andrei, Nishioka, Kenichi, Erdjument-Bromage, Hediye, Tempst, Paul, Reinberg, Danny

Enhancer of Zeste [E(z)] is a Polycomb-group transcriptional repressor and one of the founding members of the family of SET domain-containing proteins. Several SET-domain proteins possess intrinsic...

Facile synthesis of site-specifically acetylated and methylated histone proteins: Reagents for evaluation of the histone code hypothesis

He, Shu, Bauman, David, Davis, Jamaine S., Loyola, Alejandra, Nishioka, Kenichi, Gronlund, Jennifer L., ...

The functional capacity of genetically encoded histone proteins can be powerfully expanded by posttranslational modification. A growing body of biochemical and genetic evidence clearly links the...

Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation

Nishioka, Kenichi, Chuikov, Sergei, Sarma, Kavitha, Erdjument-Bromage, Hediye, Allis, C. David, Tempst, Paul, ...

A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of...

Expression and Differential Intracellular Localization of Two Major Forms of Human 8-Oxoguanine DNA Glycosylase Encoded by Alternatively Spliced OGG1 mRNAs

Nishioka, Kenichi, Ohtsubo, Toshio, Oda, Hisanobu, Fujiwara, Toshiyuki, Kang, Dongchon, Sugimachi, Keizo, ...

We identified seven alternatively spliced forms of human 8-oxoguanine DNA glycosylase (OGG1) mRNAs, classified into two types based on their last exons (type 1 with exon 7: 1a and 1b; type 2 with...

Human APE2 protein is mostly localized in the nuclei and to some extent in the mitochondria, while nuclear APE2 is partly associated with proliferating cell nuclear antigen

Tsuchimoto, Daisuke, Sakai, Yasunari, Sakumi, Kunihiko, Nishioka, Kenichi, Sasaki, Masafumi, Fujiwara, Toshiyuki, ...

In human cells APE1 is the major AP endonuclease and it has been reported to have no functional mitochondrial targeting sequence (MTS). We found that APE2 protein possesses a putative MTS. When its...

Identification of human MutY homolog (hMYH) as a repair enzyme for 2-hydroxyadenine in DNA and detection of multiple forms of hMYH located in nuclei and mitochondria

Ohtsubo, Toshio, Nishioka, Kenichi, Imaiso, Yasuyuki, Iwai, Shigenori, Shimokawa, Hidetoshi, Oda, Hisanobu, ...

An enzyme activity introducing an alkali-labile site at 2-hydroxyadenine (2-OH-A) in double-stranded oligonucleotides was detected in nuclear extracts of Jurkat cells. This activity co-eluted with...

Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation

Nishioka, Kenichi, Chuikov, Sergei, Sarma, Kavitha, Erdjument-Bromage, Hediye, Allis, C. David, Tempst, Paul, ...

A novel histone methyltransferase, termed Set9, was isolated from human cells. Set9 contains a SET domain, but lacks the pre- and post-SET domains. Set9 methylates specifically lysine 4 (K4) of...

Mitotic-specific methylation of histone H4 Lys 20 follows increased PR-Set7 expression and its localization to mitotic chromosomes

Rice, Judd C., Nishioka, Kenichi, Sarma, Kavitha, Steward, Ruth, Reinberg, Danny, Allis, C. David

We describe distinct patterns of histone methylation during human cell cycle progression. Histone H4 methyltransferase activity was found to be cell cycle-regulated, consistent with increased H4 Lys...

Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein

Kuzmichev, Andrei, Nishioka, Kenichi, Erdjument-Bromage, Hediye, Tempst, Paul, Reinberg, Danny

Enhancer of Zeste [E(z)] is a Polycomb-group transcriptional repressor and one of the founding members of the family of SET domain-containing proteins. Several SET-domain proteins possess intrinsic...

Facile synthesis of site-specifically acetylated and methylated histone proteins: Reagents for evaluation of the histone code hypothesis

He, Shu, Bauman, David, Davis, Jamaine S., Loyola, Alejandra, Nishioka, Kenichi, Gronlund, Jennifer L., ...

The functional capacity of genetically encoded histone proteins can be powerfully expanded by posttranslational modification. A growing body of biochemical and genetic evidence clearly links the...

Drosophila GAGA factor directs histone H3.3 replacement that prevents the heterochromatin spreading

Nakayama, Takahiro, Nishioka, Kenichi, Dong, Yi-Xin, Shimojima, Tsukasa, Hirose, Susumu

Epigenetic maintenance of the expression state of the genome is critical for development. Drosophila GAGA factor interacts with FACT and modulates chromatin structure for the maintenance of gene...