David J. Mitola, Hannah Aaronson, Lars Kjøller, Jørgen K. Larsen, Kenneth M. Yamada, Dudley K. Strickl, ...
uPARAP/Endo180 is essential for cellular uptake of collagen and promotes fibroblast collagen adhesion
Using HSV-Thymidine Kinase for Safety in an Allogeneic Salivary Graft Cell Line (2001)
Aframian, Doron J., Zheng, Changyu, Goldsmith, Corinne M., Nikolovski, Janeta, Cukierman, Edna, Yamada, Kenneth M., ...
Extreme salivary hypofunction is a result of tissue damage caused by irradiation therapy for cancer in the head and neck region. Unfortunately, there is no currently satisfactory treatment for this...
Molecular diversity of cell-matrix adhesions (1999)
Zamir, Eli, Katz, Ben-Zion, Aota, Shin-Ichi, Yamada, Kenneth M., Geiger, Benjamin, Kam, Zvi
In this study we have examined for molecular heterogeneity of cell-matrix adhesions and the involvement of actomyosin contractility in the selective recruitment of different plaque proteins. For this...
PTEN Gene and Integrin Signaling in Cancer (1999)
Tamura, Masahito, Gu, Jianguo, Tran, Huan, Yamada, Kenneth M.
Integrins are major adhesion- and signaling-receptor proteins that mediate cell migration and invasion. They also trigger a variety of signal transduction pathways and regulate cytoskeletal...
Immunological Characterization of Human Vitronectin and Its Binding to Glycosaminoglycans (1986)
AKAMA, Takao, YAMADA, Kenneth M., SENO, Nobuko, KASHIWAGI, Heihachira, FUNAKI, Tomoyuki, HAYASHI, Masao
The cell-adhesive glycoprotein vitronectin in human plasma was characterized with a monospecific anti-vitronectin antibody. Vitronectin, a mixture of monomeric 75 and 65 kDa polypeptides, was found...
Partial purification and characterization of the messenger RNA for cell fibronectin (1979)
Fagan, John B., Yamada, Kenneth M., De Crombrugghe, Benoit, Pastan, Ira
Fibronectin mRNA has been partially purified by guanidine extraction, oligo-(dT)-cellulose chromatography and sucrose density gradient centrifugation. We obtain a fraction which programs a wheat germ...
Microfilaments and microtubules: roles in axon elongation and cell movement. (1971)
Thesis (Ph. D.)--Dept. of Biological Sciences, Stanford University.
Katz, Ben-Zion, Zamir, Eli, Bershadsky, Alexander, Kam, Zvi, Yamada, Kenneth M., Geiger, Benjamin
This study establishes that the physical state of the extracellular matrix can regulate integrin-mediated cytoskeletal assembly and tyrosine phosphorylation to generate two distinct types of...
Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems
Podsypanina, Katrina, Ellenson, Lora Hedrick, Nemes, Adriana, Gu, Jianguo, Tamura, Masahito, Yamada, Kenneth M., ...
Pten/Mmac1+/− heterozygous mice exhibited neoplasms in multiple organs including the endometrium, liver, prostate, gastrointestinal tract, thyroid, and thymus. Loss of the wild-type allele was...
AXON GROWTH: ROLES OF MICROFILAMENTS AND MICROTUBULES*
Yamada, Kenneth M., Spooner, Brian S., Wessells, Norman K.
The motile tips of elongating axons consist of growth cones from which microspikes protrude. Cytochalasin B causes retraction of microspikes, rounding-up of growth cones, and cessation of axon...
Isolation of a Major Cell Surface Glycoprotein from Fibroblasts
Yamada, Kenneth M., Weston, James A.
A cell surface component has been isolated in partially purified form from cultured chick embryo and chick heart fibroblasts. This glycoprotein is similar to a protein recently reported to be present...
Katz, Ben-Zion, Zamir, Eli, Bershadsky, Alexander, Kam, Zvi, Yamada, Kenneth M., Geiger, Benjamin
This study establishes that the physical state of the extracellular matrix can regulate integrin-mediated cytoskeletal assembly and tyrosine phosphorylation to generate two distinct types of...
Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems
Podsypanina, Katrina, Ellenson, Lora Hedrick, Nemes, Adriana, Gu, Jianguo, Tamura, Masahito, Yamada, Kenneth M., ...
Pten/Mmac1+/− heterozygous mice exhibited neoplasms in multiple organs including the endometrium, liver, prostate, gastrointestinal tract, thyroid, and thymus. Loss of the wild-type allele was...
AXON GROWTH: ROLES OF MICROFILAMENTS AND MICROTUBULES*
Yamada, Kenneth M., Spooner, Brian S., Wessells, Norman K.
The motile tips of elongating axons consist of growth cones from which microspikes protrude. Cytochalasin B causes retraction of microspikes, rounding-up of growth cones, and cessation of axon...
Isolation of a Major Cell Surface Glycoprotein from Fibroblasts
Yamada, Kenneth M., Weston, James A.
A cell surface component has been isolated in partially purified form from cultured chick embryo and chick heart fibroblasts. This glycoprotein is similar to a protein recently reported to be present...
Qian, Xiaolan, Li, Guorong, Asmussen, Holly K., Asnaghi, Laura, Vass, William C., Braverman, Richard, ...
The three deleted in liver cancer genes (DLC1–3) encode Rho-GTPase-activating proteins (RhoGAPs) whose expression is frequently down-regulated or silenced in a variety of human malignancies. The...
A Rac switch regulates random versus directionally persistent cell migration
Pankov, Roumen, Endo, Yukinori, Even-Ram, Sharona, Araki, Masaru, Clark, Katherine, Cukierman, Edna, ...
Directional migration moves cells rapidly between points, whereas random migration allows cells to explore their local environments. We describe a Rac1 mechanism for determining whether cell patterns...
Src-Dependent Phosphorylation of ASAP1 Regulates Podosomes▿
Bharti, Sanita, Inoue, Hiroki, Bharti, Kapil, Hirsch, Dianne S., Nie, Zhongzhen, Yoon, Hye-Young, ...
Invadopodia are Src-induced cellular structures that are thought to mediate tumor invasion. ASAP1, an Arf GTPase-activating protein (GAP) containing Src homology 3 (SH3) and Bin, amphiphysin, and...
MICROFILAMENTS AND CELL LOCOMOTION
Spooner, Brian S., Yamada, Kenneth M., Wessells, Norman K.
The role of microfilaments in generating cell locomotion has been investigated in glial cells migrating in vitro. Such cells are found to contain two types of microfilament systems: First, a sheath...
ULTRASTRUCTURE AND FUNCTION OF GROWTH CONES AND AXONS OF CULTURED NERVE CELLS
Yamada, Kenneth M., Spooner, Brian S., Wessells, Norman K.
Dorsal root ganglion nerve cells undergoing axon elongation in vitro have been analyzed ultrastructurally. The growth cone at the axonal tip contains smooth endoplasmic reticulum, vesicles,...
Savagner, Pierre, Yamada, Kenneth M., Thiery, Jean Paul
Epithelial–mesenchymal transition (EMT) is an essential morphogenetic process during embryonic development. It can be induced in vitro by hepatocyte growth factor/scatter factor (HGF/SF), or by...
Gu, Jianguo, Tamura, Masahito, Yamada, Kenneth M.
The tumor suppressor PTEN dephosphorylates focal adhesion kinase (FAK) and inhibits integrin-mediated cell spreading and cell migration. We demonstrate here that expression of PTEN selectively...
Kioka, Noriyuki, Sakata, Shohei, Kawauchi, Takeshi, Amachi, Teruo, Akiyama, Steven K., Okazaki, Kenji, ...
Using the yeast two-hybrid system and an in vitro binding assay, we have identified a novel protein termed vinexin as a vinculin-binding protein. By Northern blotting, we identified two types of...
Danen, Erik H.J., Sonneveld, Petra, Sonnenberg, Arnoud, Yamada, Kenneth M.
In cellular transformation, activated forms of the small GTPases Ras and RhoA can cooperate to drive cells through the G1 phase of the cell cycle. Here, we show that a similar but substrate-regulated...
Shc and Fak Differentially Regulate Cell Motility and Directionality Modulated by Pten
Gu, Jianguo, Tamura, Masahito, Pankov, Roumen, Danen, Erik H.J., Takino, Takahisa, Matsumoto, Kazue, ...
Cell migration is modulated by regulatory molecules such as growth factors, oncogenes, and the tumor suppressor PTEN. We previously described inhibition of cell migration by PTEN and restoration of...
Engelholm, Lars H., List, Karin, Netzel-Arnett, Sarah, Cukierman, Edna, Mitola, David J., Aaronson, Hannah, ...
The uptake and lysosomal degradation of collagen by fibroblasts constitute a major pathway in the turnover of connective tissue. However, the molecular mechanisms governing this pathway are poorly...
The relationship between force and focal complex development
Galbraith, Catherine G., Yamada, Kenneth M., Sheetz, Michael P.
To adhere and migrate, cells must be capable of applying cytoskeletal force to the extracellular matrix (ECM) through integrin receptors. However, it is unclear if connections between integrins and...
Pankov, Roumen, Cukierman, Edna, Katz, Ben-Zion, Matsumoto, Kazue, Lin, Diane C., Lin, Shin, ...
Fibronectin matrix assembly is a multistep, integrin-dependent process. To investigate the role of integrin dynamics in fibronectin fibrillogenesis, we developed an antibody-chasing technique for...
Even-Ram, Sharona, Yamada, Kenneth M
Non-muscle myosin II has diverse functions in cell contractility, morphology, cytokinesis and migration. Mammalian cells have three isoforms of non-muscle myosin II, termed IIA, IIB and IIC, encoded...
One-dimensional topography underlies three-dimensional fibrillar cell migration
Doyle, Andrew D., Wang, Francis W., Matsumoto, Kazue, Yamada, Kenneth M.
Current concepts of cell migration were established in regular two-dimensional (2D) cell culture, but the roles of topography are poorly understood for cells migrating in an oriented 3D fibrillar...