Kenneth Stuart

Quintet / (1969)

Stuart, Kenneth.

Thesis (M.A.)--University of Connecticut, 1969.

Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes

Myler, Peter J., Audleman, Lindsey, DeVos, Theo, Hixson, Greg, Kiser, Patti, Lemley, Craig, ...

Leishmania are evolutionarily ancient protozoans (Kinetoplastidae) and important human pathogens that cause a spectrum of diseases ranging from the asymptomatic to the lethal. The Leishmania genome...

Uridylate Addition and RNA Ligation Contribute to the Specificity of Kinetoplastid Insertion RNA Editing

Igo, Robert P., Palazzo, Setareh S., Burgess, Moffett L. K., Panigrahi, Aswini K., Stuart, Kenneth

RNA editing in Trypanosoma brucei inserts and deletes uridylates (U's) in mitochondrial pre-mRNAs under the direction of guide RNAs (gRNAs). We report here the development of a novel in vitro...

The unusual gene organization of Leishmania major chromosome 1 may reflect novel transcription processes

McDonagh, Paul D., Myler, Peter J., Stuart, Kenneth

The complete chromosomal sequence for chromosome 1 from Leishmania major Friedlin predicts that this chromosome has 79 protein-coding genes. Surprisingly, the first 29 of these genes are encoded in...

Role of Uridylate-Specific Exoribonuclease Activity in Trypanosoma brucei RNA Editing

Igo, Robert P., Weston, David S., Ernst, Nancy Lewis, Panigrahi, Aswini K., Salavati, Reza, Stuart, Kenneth

Editing of mitochondrial mRNAs in kinetoplastid protozoa occurs by a series of enzymatic steps that insert and delete uridylates (U's) as specified by guide RNAs (gRNAs). The characteristics of the...

TbMP81 is required for RNA editing in Trypanosoma brucei

Drozdz, Maciej, Palazzo, Setareh S., Salavati, Reza, O’Rear, Jeff, Clayton, Christine, Stuart, Kenneth

Most mitochondrial mRNAs are edited in Trypano soma brucei by a series of steps that are catalyzed by a multienzyme complex that is in its initial stages of characterization. RNA interference...

RNA Sequence and Base Pairing Effects on Insertion Editing in Trypanosoma brucei

Igo, Robert P., Lawson, Sobomabo D., Stuart, Kenneth

RNA editing inserts and deletes uridylates (U's) in kinetoplastid mitochondrial pre-mRNAs by a series of enzymatic steps. Small guide RNAs (gRNAs) specify the edited sequence. Editing, though...

TbMP44 Is Essential for RNA Editing and Structural Integrity of the Editosome in Trypanosoma brucei

Wang, Bingbing, Ernst, Nancy Lewis, Palazzo, Setareh S., Panigrahi, Aswini K., Salavati, Reza, Stuart, Kenneth

RNA editing produces mature mitochondrial mRNAs in trypanosomatids by the insertion and deletion of uridylates. It is catalyzed by a multiprotein complex, the editosome. We identified TbMP44 among...

SURVEY AND SUMMARY: Comparative analysis of editosome proteins in trypanosomatids

Worthey, Elizabeth A., Schnaufer, Achim, Mian, I. Saira, Stuart, Kenneth, Salavati, Reza

Detailed comparisons of 16 editosome proteins from Trypanosoma brucei, Trypanosoma cruzi and Leishmania major identified protein motifs associated with catalysis and protein or nucleic acid...

Transcription Initiation and Termination on Leishmania major Chromosome 3

Martínez-Calvillo, Santiago, Nguyen, Dan, Stuart, Kenneth, Myler, Peter J.

Genome projects involving Leishmania and other trypanosomatids have revealed that most genes in these organisms are organized into large clusters of genes on the same DNA strand. We have previously...

A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing.

Wang, Bingbing, Salavati, Reza, Heidmann, Stefan, Stuart, Kenneth

Current in vitro assays for RNA editing in kinetoplastids directly examine the products generated by incubation of pre-mRNA substrate with guide RNA (gRNA) and mitochondrial (mt) extract. RNA editing...

Identification of novel components of Trypanosoma brucei editosomes

PANIGRAHI, ASWINI K., SCHNAUFER, ACHIM, ERNST, NANCY L., WANG, BINGBING, CARMEAN, NICOLE, SALAVATI, REZA, ...

The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome...

An essential RNase III insertion editing endonuclease in Trypanosoma brucei

Carnes, Jason, Trotter, James Raffaello, Ernst, Nancy Lewis, Steinberg, Alodie, Stuart, Kenneth

RNA editing adds and deletes uridine nucleotides in many preedited mRNAs to create translatable mRNAs in the mitochondria of the parasite Trypanosoma brucei. Kinetoplastid RNA editing protein B3...

KREPA4, an RNA binding protein essential for editosome integrity and survival of Trypanosoma brucei

Salavati, Reza, Ernst, Nancy Lewis, O'Rear, Jeff, Gilliam, Troy, Tarun, Salvador, Stuart, Kenneth

The 20S editosome, a multiprotein complex, catalyzes the editing of most mitochondrial mRNAs in trypanosomatids by uridylate insertion and deletion. RNAi mediated inactivation of expression of KREPA4...

Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes

Myler, Peter J., Audleman, Lindsey, DeVos, Theo, Hixson, Greg, Kiser, Patti, Lemley, Craig, ...

Leishmania are evolutionarily ancient protozoans (Kinetoplastidae) and important human pathogens that cause a spectrum of diseases ranging from the asymptomatic to the lethal. The Leishmania genome...

Uridylate Addition and RNA Ligation Contribute to the Specificity of Kinetoplastid Insertion RNA Editing

Igo, Robert P., Palazzo, Setareh S., Burgess, Moffett L. K., Panigrahi, Aswini K., Stuart, Kenneth

RNA editing in Trypanosoma brucei inserts and deletes uridylates (U's) in mitochondrial pre-mRNAs under the direction of guide RNAs (gRNAs). We report here the development of a novel in vitro...

The unusual gene organization of Leishmania major chromosome 1 may reflect novel transcription processes

McDonagh, Paul D., Myler, Peter J., Stuart, Kenneth

The complete chromosomal sequence for chromosome 1 from Leishmania major Friedlin predicts that this chromosome has 79 protein-coding genes. Surprisingly, the first 29 of these genes are encoded in...

Role of Uridylate-Specific Exoribonuclease Activity in Trypanosoma brucei RNA Editing

Igo, Robert P., Weston, David S., Ernst, Nancy Lewis, Panigrahi, Aswini K., Salavati, Reza, Stuart, Kenneth

Editing of mitochondrial mRNAs in kinetoplastid protozoa occurs by a series of enzymatic steps that insert and delete uridylates (U's) as specified by guide RNAs (gRNAs). The characteristics of the...

TbMP81 is required for RNA editing in Trypanosoma brucei

Drozdz, Maciej, Palazzo, Setareh S., Salavati, Reza, O’Rear, Jeff, Clayton, Christine, Stuart, Kenneth

Most mitochondrial mRNAs are edited in Trypano soma brucei by a series of steps that are catalyzed by a multienzyme complex that is in its initial stages of characterization. RNA interference...

RNA Sequence and Base Pairing Effects on Insertion Editing in Trypanosoma brucei

Igo, Robert P., Lawson, Sobomabo D., Stuart, Kenneth

RNA editing inserts and deletes uridylates (U's) in kinetoplastid mitochondrial pre-mRNAs by a series of enzymatic steps. Small guide RNAs (gRNAs) specify the edited sequence. Editing, though...

TbMP44 Is Essential for RNA Editing and Structural Integrity of the Editosome in Trypanosoma brucei

Wang, Bingbing, Ernst, Nancy Lewis, Palazzo, Setareh S., Panigrahi, Aswini K., Salavati, Reza, Stuart, Kenneth

RNA editing produces mature mitochondrial mRNAs in trypanosomatids by the insertion and deletion of uridylates. It is catalyzed by a multiprotein complex, the editosome. We identified TbMP44 among...

SURVEY AND SUMMARY: Comparative analysis of editosome proteins in trypanosomatids

Worthey, Elizabeth A., Schnaufer, Achim, Mian, I. Saira, Stuart, Kenneth, Salavati, Reza

Detailed comparisons of 16 editosome proteins from Trypanosoma brucei, Trypanosoma cruzi and Leishmania major identified protein motifs associated with catalysis and protein or nucleic acid...

Transcription Initiation and Termination on Leishmania major Chromosome 3

Martínez-Calvillo, Santiago, Nguyen, Dan, Stuart, Kenneth, Myler, Peter J.

Genome projects involving Leishmania and other trypanosomatids have revealed that most genes in these organisms are organized into large clusters of genes on the same DNA strand. We have previously...

An essential RNase III insertion editing endonuclease in Trypanosoma brucei

Carnes, Jason, Trotter, James Raffaello, Ernst, Nancy Lewis, Steinberg, Alodie, Stuart, Kenneth

RNA editing adds and deletes uridine nucleotides in many preedited mRNAs to create translatable mRNAs in the mitochondria of the parasite Trypanosoma brucei. Kinetoplastid RNA editing protein B3...

A hammerhead ribozyme substrate and reporter for in vitro kinetoplastid RNA editing.

Wang, Bingbing, Salavati, Reza, Heidmann, Stefan, Stuart, Kenneth

Current in vitro assays for RNA editing in kinetoplastids directly examine the products generated by incubation of pre-mRNA substrate with guide RNA (gRNA) and mitochondrial (mt) extract. RNA editing...

Identification of novel components of Trypanosoma brucei editosomes

PANIGRAHI, ASWINI K., SCHNAUFER, ACHIM, ERNST, NANCY L., WANG, BINGBING, CARMEAN, NICOLE, SALAVATI, REZA, ...

The editosome is a multiprotein complex that catalyzes the insertion and deletion of uridylates that occurs during RNA editing in trypanosomatids. We report the identification of nine novel editosome...

KREPA4, an RNA binding protein essential for editosome integrity and survival of Trypanosoma brucei

Salavati, Reza, Ernst, Nancy Lewis, O'Rear, Jeff, Gilliam, Troy, Tarun, Salvador, Stuart, Kenneth

The 20S editosome, a multiprotein complex, catalyzes the editing of most mitochondrial mRNAs in trypanosomatids by uridylate insertion and deletion. RNAi mediated inactivation of expression of KREPA4...

An essential role of KREPB4 in RNA editing and structural integrity of the editosome in Trypanosoma brucei

Babbarwal, Vinod Kumar, Fleck, Michele, Ernst, Nancy Lewis, Schnaufer, Achim, Stuart, Kenneth

RNA editing in the sleeping sickness parasite Trypanosoma brucei remodels mitochondrial transcripts by the addition and deletion of uridylates as specified by guide RNAs. Editing is catalyzed by at...

RNA Editing in Trypanosoma brucei Requires Three Different Editosomes▿ †

Carnes, Jason, Trotter, James Raffaello, Peltan, Adam, Fleck, Michele, Stuart, Kenneth

Trypanosoma brucei has three distinct ∼20S editosomes that catalyze RNA editing by the insertion and deletion of uridylates. Editosomes with the KREN1 or KREN2 RNase III type endonucleases...

KREPA6 is an RNA-binding protein essential for editosome integrity and survival of Trypanosoma brucei

Tarun, Salvador Zipagan, Schnaufer, Achim, Ernst, Nancy Lewis, Proff, Rosemary, Deng, Junpeng, Hol, Wim, ...

Most mitochondrial mRNAs in kinetoplastid protozoa require post-transcriptional RNA editing that inserts and deletes uridylates, a process that is catalyzed by multiprotein editosomes. KREPA6 is the...