Kevin M. Myles, Dennis J. Pierro, Ken E. Olson
Within mosquitoes, arboviruses encounter barriers to infection and dissemination that are critical determinants of vector competence. The molecular mechanisms responsible for these barriers have yet...
Myles, Kevin M., Pierro, Dennis J., Olson, Ken E.
The Sindbis virus (Alphavirus; Togaviridae) strain MRE16 efficiently infects Aedes aegypti mosquitoes that ingest a blood meal containing 8 to 9 log10 PFU of virus/ml. However, a small-plaque variant...
Myles, Kevin M., Kelly, Cindy L. H., Ledermann, Jeremy P., Powers, Ann M.
The genomic RNA of an alphavirus encodes four different nonstructural proteins, nsP1, nsP2, nsP3, and nsP4. The polyprotein P123 is produced when translation terminates at an opal termination codon...
Myles, Kevin M., Pierro, Dennis J., Olson, Ken E.
The Sindbis virus (Alphavirus; Togaviridae) strain MRE16 efficiently infects Aedes aegypti mosquitoes that ingest a blood meal containing 8 to 9 log10 PFU of virus/ml. However, a small-plaque variant...
Myles, Kevin M., Kelly, Cindy L. H., Ledermann, Jeremy P., Powers, Ann M.
The genomic RNA of an alphavirus encodes four different nonstructural proteins, nsP1, nsP2, nsP3, and nsP4. The polyprotein P123 is produced when translation terminates at an opal termination codon...
Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
Myles, Kevin M., Wiley, Michael R., Morazzani, Elaine M., Adelman, Zach N.
Mosquito-borne viruses cause significant levels of morbidity and mortality in humans and domesticated animals. Maintenance of mosquito-borne viruses in nature requires a biological transmission cycle...
Accurate Strand-Specific Quantification of Viral RNA
Plaskon, Nicole E., Adelman, Zach N., Myles, Kevin M.
The presence of full-length complements of viral genomic RNA is a hallmark of RNA virus replication within an infected cell. As such, methods for detecting and measuring specific strands of viral RNA...