Kimimitsu Oda

Warfarin administration disrupts the assembly of mineralized nodules in the osteoid (2009)

Amizuka, Norio, Li, Minqi, Hara, Kuniko, Kobayashi, Masatoshi, Ubaidus, Sobhan, ...

This study aimed to elucidate the ultrastructural role of Gla proteins in bone mineralization by means of a warfarin-administration model. Thirty-six 4-week-old male F344 rats received warfarin...

FGF23 is mainly synthesized by osteocytes in the regularly distributed osteocytic lacunar canalicular system established after physiological bone remodeling (2009)

Ubaidus, Sobhan, Li, Minqi, Sultana, Sara, Oda, Kimimitsu, Maeda, Takeyasu, ...

This study aimed to evaluate whether the immunolocalization of fibroblast growth factor (FGF) 23 and dentin matrix protein 1 (DMP1) is associated with the spatial regularity of the osteocyte lacunar...

Functional Expression of Furin Demonstrating Its Intracellular Localization and Endoprotease Activity for Processing of Proalbumin and Complement Pro-C3* (2008)

Yoshio Misumi, Kimimitsu Oda, Toshiyuki Fujiwara, Noboru Takami, Kosuke Tashiros, Yukio Ikeharas

We have cloned a rat cDNA encoding furin which is to isolate and characterize endoproteases involved in the structurally related to yeast Kex2 protease. Products processing of these precursors (3, 7,...

Defective bone remodelling in osteoprotegerin-deficient mice (2003)

Amizuka, Norio, Shimomura, Junko, Li, Minqi, Seki, Yukie, Oda, Kimimitsu, Henderson, Janet E., ...

Previous studies have reported enhanced osteoclastogenesis, increased bone resorption and osteoporosis in osteoprotegerin (OPG)-deficient mice. In the present study, we show that the tibial epiphyses...

Tissue-Nonspecific Alkaline Phosphatase with an Asp289->Val Mutation Fails to Reach the Cell Surface and Undergoes Proteasome-Mediated Degradation (2003)

Ishida, Yoko, Komaru, Keiichi, Ito, Masahiro, Amaya, Yoshihiro, Kohno, Shoji, Oda, Kimimitsu

A missense mutation in the gene of tissue-nonspecific alkaline phosphatase, which replaces aspartic acid at position 289 with valine [TNSALP (D289V)], was reported in a lethal...

A General Method for Rapid Purification of Soluble Versions of Glycosyhosphatidylinositol-Anchored Proteins Expressed in Insect Cells: An Application for Human Tissue-Nonspecific Alkaline Phosphatase (1999)

Oda, Kimimitsu, Amaya, Yoshihiro, Fukushi-Irie, Mariko, Kinameri, Yasuko, Ohsuye, Kazuhiro, Kubota, Ichiro, ...

A soluble form of tissue-nonspecific alkaline phosphatase was purified to apparent homogeneity from the culture media of Sf9 cells which had been infected with recombinant baculoviruses encoding...

Basement Membrane Heparan Sulfate Proteoglycan (Perlecan) Synthesized by ACC3, Adenoid Cystic Carcinoma Cells of Human Salivary Gland Origin (1999)

Kimura, Shinn, Cheng, Jun, Toyoshima, Koh-éi, Oda, Kimimitsu, Saku, Takashi

The biosynthesis of basement membrane heparan sulfate proteoglycan (HSPG), known as perlecan, in ACC3 cells established from a adenoid cystic carcinoma of the human salivary gland was studied using...

Defective Intracellular Transport of Tissue-Nonspecific Alkaline Phosphatase with an Ala162->Thr Mutation Associated with Lethal Hypophosphatasia (1998)

Shibata, Hisanobu, Fukushi, Mariko, Igarashi, Atsuko, Misumi, Yoshio, Ikehara, Yukio, Ohashi, Yasushi, ...

We have studied the biosynthesis and intracellular transport of tissue-nonspecific alkaline phosphatase (TNSALP) transiently expressed in COS-1 cells. Mutations were introduced into TNSALP to examine...

Intracellular Sorting of Lysosomal {beta}-Glucuronidase Is Altered Due to Administaration of Dibutyl Phosphate (1995)

Nishimura, Yukio, Kato, Keitaro, Oda, Kimimitsu, Ishikawa, Toyoko, Ikehara, Yukio, Himeno, Masaru

Organophosphate compounds are known to cause a selective increase of β-glucuronidase activity in rat serum. Previous data suggested that increase of serum β-glucuronidase activity was well...

Molecular Cloning and Sequencing of the cDNA of Rat {alpha}1-Protease Inhibitor and Its Expression in COS-1 Cells (1990)

Misumi, Yoshio, Sohda, Miwa, Ohkubo, Kumiko, Takami, Noboru, Oda, Kimimitsu, Ikehara, Yukio

A cDNA clone for α1-protease inhibitor (pcα1PI212) was isolated from a γZAP rat liver cDNA library. The 1.4 kb cDNA insert of pcα1PI212 contained an open reading frame that encodes a 411-residue...

Production of Propeptide-Directed Antibody: Its Application to the Purification of Proalbumin and Analysis of Proalbumin Processing (1990)

Oda, Kimimitsu, Fujiwara, Toshiyuki, Ogata, Shigenori, Ikehara, Yukio

Antibodies which specifically recognize rat proalbumin, but not mature serum-albumin, were raised. Propeptide (NH2-Arg-Gly-Val-Phe-Arg-Arg) with an additional cysteine residue at the carboxyl...

Putative Convertase Involved in the Proteolytic Conversion of Rat Proalbumin to Serum Albumin (1988)

Oda, Kimimitsu, Ikehara, Yukio

We have found a proteolytic activity in Golgi membranes which efficiently converts [35S]methionine-labeled proalbumin, isolated from pulse-labeled rat hepatocytes in culture, to serum albumin in an...

Immunoblotting Analysis of Plasma Protein Processing in the Secretory Pathway of Rat liver: Identification of Proteolytic Conversion Sites of Complement Pro-C3 and Prohaptoglobin (1986)

ODA, Kimimitsu, MIKI, Koichiro, HIROSE, Shinichi, TAKAMI, Noboru, MISUMI, Yoshio, IKEHARA, Yukio

Using an immunoblotting technique, we have studied the processing of plasma proteins in subcellular fractions of rat liver including rough and smooth micro-somes and the Golgi subtractions. Each...

Induction of Alkaline Phosphatase and Its Transport to Cell Surface in Primary Culture of Rat Hepatocytes: Effect of the Antimicrotubular Agent Colchicine (1986)

MISUMI, Yoshio, ODA, Kimimitsu, IKEHARA, Yukio

We have examined the effect of colchicine on the induction of alkaline phosphatase and its transport to the cell surface in a primary culture of rat hepatocytes. When freshly isolated hepatocytes...

Induction of Rat Hepatic Alkaline Phosphatase and Its Appearance in Serum: Electrophoretic Characterization of Liver-Membranous and Serum-Soluble Forms (1984)

KOMINAMI, Tatsuya, ODA, Kimimitsu, IKEHARA, Yukio

Simultaneous bile duct ligation and colchicine injection (2 mg/kg body weight) in rats caused a remarkable induction of alkaline phosphatase in the liver. Concomitantly, a marked elevation of the...

Precursor of Rat Liver {alpha}2u-Globulin: Partial Amino Acid Sequence Determination of Its Signal Peptide (1981)

IKEHARA, Yukio, ODA, Kimimitsu, ROSENFELD, Melvin G., BAR-NUN, Shoshana, KREIBICH, Gert

The biosynthesis of α2u-globulin, the major protein in rat urine, was studied in a cell-free system programmed with messenger RNA isolated from rat liver. Analysis of the primary translation product...

An Improved Method for the Purification of Rat Serum Albumin: Removal of Contaminants by Concanavalin A-Sepharose (1977)

IKEHARA, Yukio, ODA, Kimimitsu, KATO, Keitaro

Minor contaminants occasionally found in conventionally prepared rat serum albumin were easily and completely removed by concanavalin A-Sepharose chromatography. The unadsorbed fraction from a...

Acceptor Activity of Subcellular Membranes for Two Terminal Sugars, Galactose and Sialic Acid (1977)

IKEHARA, Yukio, ODA, Kimimitsu, KATO, Keitaro

The acceptor activities of subcellular membrane preparations for the terminal sugars, galactose and slalic acid, were compared using a Golgi fraction purified from rat liver as an exogenous emzyme...

Retention at the cis-Golgi and delayed degradation of tissue-non-specific alkaline phosphatase with an Asn153-->Asp substitution, a cause of perinatal hypophosphatasia.

Ito, Masahiro, Amizuka, Norio, Ozawa, Hidehiro, Oda, Kimimitsu

Tissue-non-specific alkaline phosphatase (TNSALP) is an ectoenzyme anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). A TNSALP mutant with an Asn(153)-->Asp (N153D) substitution...

Retention at the cis-Golgi and delayed degradation of tissue-non-specific alkaline phosphatase with an Asn153-->Asp substitution, a cause of perinatal hypophosphatasia.

Ito, Masahiro, Amizuka, Norio, Ozawa, Hidehiro, Oda, Kimimitsu

Tissue-non-specific alkaline phosphatase (TNSALP) is an ectoenzyme anchored to the plasma membrane via glycosylphosphatidylinositol (GPI). A TNSALP mutant with an Asn(153)-->Asp (N153D) substitution...

The Cellular Prion Protein Interacts with the Tissue Non-Specific Alkaline Phosphatase in Membrane Microdomains of Bioaminergic Neuronal Cells

Ermonval, Myriam, Baudry, Anne, Baychelier, Florence, Pradines, Elodie, Pietri, Mathéa, Oda, Kimimitsu, ...

Identification of cell cycle–arrested quiescent osteoclast precursors in vivo

Mizoguchi, Toshihide, Muto, Akinori, Udagawa, Nobuyuki, Arai, Atsushi, Yamashita, Teruhito, Hosoya, Akihiro, ...

Osteoclasts are multinucleated cells that resorb bone. Although osteoclasts originate from the monocyte/macrophage lineage, osteoclast precursors are not well characterized in vivo. The relationship...