Kiyoshi KITA

Gain and loss of an intron in a protein-coding gene in Archaea: the case of an archaeal RNA pseudouridine synthase gene (2009)

Yokobori, Shin-ichi, Itoh, Takashi, Yoshinari, Shigeo, Nomura, Norimichi, Sako, Yoshihiko, Yamagishi, Akihiko, ...

Abstract Background We previously found the first examples of splicing of archaeal pre-mRNAs for homologs of the eukaryotic CBF5 protein (also known as dyskerin in humans) in Aeropyrum pernix,...

Mitochondrial Dehydrogenases in the Aerobic Respiratory Chain of the Rodent Malaria Parasite Plasmodium yoelii yoelii (2009)

Kawahara, Kenji, Mogi, Tatsushi, Tanaka, Takeshi Q, Hata, Masayuki, Miyoshi, Hideto, Kita, Kiyoshi

In the intraerythrocytic stages of malaria parasites, mitochondria lack obvious cristae and are assumed to derive energy through glycolysis. For understanding of parasite energy metabolism in...

Siccanin Rediscovered as a Species-Selective Succinate Dehydrogenase Inhibitor (2009)

Mogi, Tatsushi, Kawakami, Takuro, Arai, Hiroyuki, Igarashi, Yasuo, Matsushita, Kazunobu, Mori, Mihoko, ...

To identify antibiotics targeting to respiratory enzymes, we carried out matrix screening of a structurally varied natural compound library with Pseudomonas aeruginosa membrane-bound respiratory...

Biochemical and Spectroscopic Properties of Cyanide-Insensitive Quinol Oxidase from Gluconobacter oxydans (2009)

Mogi, Tatsushi, Ano, Yoshitaka, Nakatsuka, Tomoko, Toyama, Hirohide, Muroi, Atsushi, Miyoshi, Hideto, ...

Cyanide-insensitive quinol oxidase (CioAB), a relative of cytochrome bd, has no spectroscopic features of hemes b595 and d in the wild-type bacteria and is difficult to purify for detailed...

Functional importance of Crenarchaea-specific extra-loop revealed by an X-ray structure of a heterotetrameric crenarchaeal splicing endonuclease (2009)

Yoshinari, Shigeo, Shiba, Tomoo, Inaoka, Daniel-Ken, Itoh, Takashi, Kurisu, Genji, Harada, Shigeharu, ...

Archaeal splicing endonucleases (EndAs) are currently classified into three groups. Two groups require a single subunit protein to form a homodimer or homotetramer. The third group requires two...

A Cryptic Algal Group Unveiled: A Plastid Biosynthesis Pathway in the Oyster Parasite Perkinsus marinus (2008)

Matsuzaki, Motomichi, Kuroiwa, Haruko, Kuroiwa, Tsuneyoshi, Kita, Kiyoshi, Nozaki, Hisayoshi

Plastids are widespread in plant and algal lineages. They are also exploited by some nonphotosynthetic protists, including malarial parasites, to support their diverse modes of life. However, cryptic...

Cloning and Characterization of Ferredoxin and Ferredoxin-NADP+ Reductase from Human Malaria Parasite (2007)

Kimata-Ariga, Yoko, Kurisu, Genji, Kusunoki, Masami, Aoki, Sayaka, Sato, Dan, Kobayashi, Tamaki, ...

The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite's survival. We cloned...

Cloning and Characterization of Ferredoxin and Ferredoxin-NADP+ Reductase from Human Malaria Parasite (2007)

Kimata-Ariga, Yoko, Kurisu, Genji, Kusunoki, Masami, Aoki, Sayaka, Sato, Dan, Kobayashi, Tamaki, ...

The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite’s survival. We...

Cloning and Characterization of Ferredoxin and Ferredoxin-NADP+ Reductase from Human Malaria Parasite (2007)

Kimata-Ariga, Yoko, Kurisu, Genji, Kusunoki, Masami, Aoki, Sayaka, Sato, Dan, Kobayashi, Tamaki, ...

The human malaria parasite (Plasmodium falciparum) possesses a plastid-derived organelle called the apicoplast, which is believed to employ metabolisms crucial for the parasite’s survival. We...

An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu (2006)

Arita, Masashi, Suematsu, Takuma, Osanai, Arihiro, Inaba, Takashi, Kamiya, Haruo, Kita, Kiyoshi, ...

EF-Tu delivers aminoacyl-tRNAs to ribosomes in the translation system. However, unusual truncations found in some animal mitochondrial tRNAs seem to prevent recognition by a canonical EF-Tu. We...

Up-Regulation of Heme Biosynthesis during Differentiation of Neuro2a Cells (2006)

Shinjyo, Noriko, Kita, Kiyoshi

Heme is an iron-containing tetrapyrrole molecule that functions as a prosthetic group for proteins such as mitochondrial respiratory enzymes. Several studies have suggested that heme has essential...

An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu (2006)

Arita, Masashi, Suematsu, Takuma, Osanai, Arihiro, Inaba, Takashi, Kamiya, Haruo, Kita, Kiyoshi, ...

EF-Tu delivers aminoacyl-tRNAs to ribosomes in the translation system. However, unusual truncations found in some animal mitochondrial tRNAs seem to prevent recognition by a canonical EF-Tu. We...

An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu (2006)

Arita, Masashi, Suematsu, Takuma, Osanai, Arihiro, Inaba, Takashi, Kamiya, Haruo, Kita, Kiyoshi, ...

EF-Tu delivers aminoacyl-tRNAs to ribosomes in the translation system. However, unusual truncations found in some animal mitochondrial tRNAs seem to prevent recognition by a canonical EF-Tu. We...

Functional reconstitution of an archaeal splicing endonuclease in vitro (2005)

Yoshinari, Shigeo, Fujita, Shinji, Masui, Ryoji, Kuramitsu, Seiki, Yokobori, Shin-ichi, Kita, Kiyoshi, ...

Sulfolobus tokodaii strain 7 is one of archaea whose entire genome has been sequenced. The genome sequence revealed that it possesses two open reading frames (ORFs) that are homologous to endA, a...

Direct Evidence for Two Distinct Forms of the Flavoprotein Subunit of Human Mitochondrial Complex II (Succinate-Ubiquinone Reductase) (2003)

Tomitsuka, Eriko, Hirawake, Hiroko, Goto, Yu-ichi, Taniwaki, Masafumi, Harada, Shigeharu, Kita, Kiyoshi

Succinate-ubiquinone reductase (complex II) is an important enzyme complex in both the tricarboxylic acid cycle and aerobic respiration. A recent study showed that defects in human complex II are...

Automated Chemical Synthesis of Biologically Active tRNA Having a Sequence Corresponding to Ascaris suum Mitochondrial tRNAMet toward NMR Measurements (1996)

Ohtsuki, Takashi, Vinayak, Ravi, Watanabe, Yoh-ichi, Kita, Kiyoshi, Kawai, Gota, Watanabe, Kimitsuna

RNA samples corresponding to Ascaris suum mitochondrial tRNAMet were chemically and automatically synthesized in amounts sufficient for NMR measurement. Conventional and rapid deprotection methods...

Purification of Low-Molecular-Weight GTP-Binding Proteins Structurally Related to MTG33, a Mitochondrial GTP-Binding Protein of Ehrlich Ascites Tumor Cells (1995)

Takeda, Shizu, Kita, Kiyoshi, Miyazaki, Hideyo, Natori, Shunji, Sekimizu, Kazuhisa

We purified a mitochondrial GTP-binding protein, MTG33, from a particulate fraction of Ehrlich ascites tumor cells [Takeda, S., Sagara, Y., Kita, K., Natori, S., and Sekimizu, K. (1993) J. Biochem....

Lymphocyte Isoforms of Mouse p50 LSP1, Which Are Phosphorylated in Mitogen-Activated T Cells, Are Formed through Alternative Splicing and Phosphorylation (1995)

Matsumoto, Naoki, Kita, Kiyoshi, Kojima, Somei, Yamamoto, Kazuo, Irimura, Tatsuro, Miyagi, Masaru, ...

p50 is phosphorylated in mitogen-stimulated T cells, and translocated from the membrane to the cytosol after activation of protein kinase C. Sequence analysis of p50 revealed that it is identical...

Demonstration of the target molecule of a protective IgE antibody in secretory glands of Schistosoma japonicum larvae (1994)

Nara, Takeshi, Matsumoto, Naoki, Janecharut, Tuenta, Matsuda, Hajime, Yamamoto, Kazuo, Irimura, Tatsuro, ...

We have demonstrated that a mouse monocional IgE antibody, SJ18ϵ, recognizes a 97 kDa surface molecule (Sj97) of Schistosoma japonlcum larvae and that the antibody Induces partial but...

Human Complex II (Succinate-Ubiquinone Oxidoreductase): cDNA Cloning of the Flavoprotein (Fp) Subunit of Liver Mitochondria (1994)

Hirawake, Hiroko, Wang, Hua, Kuramochi, Toshiaki, Kojima, Somei, Kita, Kiyoshi

Complex II (succinate-ubiquinone oxidoreductase) is an important enzyme complex in both the tricarboxylic acid cycle, and the aerobic respiratory chains of mitochondria in eukaryotic cells and...

Purification of a GTP-Binding Protein Localized in Mitochondria (1993)

Takeda, Shizu, Sagara, Yasuhiro, Kita, Kiyoshi, Natori, Shunji, Sekimizu, Kazuhisa

A participate fraction consisting of heavy organelles such as nuclei and mitochondria was prepared from Ehrlich ascites tumor cells. From this fraction we have purified a GTP-binding protein with a...

Purification and Properties of a Cytocbrome b560-d Complex, a Terminal Oxidase of the Aerobic Respiratory Chain of Photobacterium phosphoreum (1986)

KONISHI, Kiyoshi, OUCHI, Masato, KITA, Kiyoshi, HORIKOSHI, Isamu

A cytochrome b560-d complex, a terminal oxidase in the respiratory chain of Photobacterium phosphoreum grown under aerobic conditions, was purified to near homogeneity. The purified oxidase complex...

An anthelmintic compound, nafuredin, shows selective inhibition of complex I in helminth mitochondria

Ōmura, Satoshi, Miyadera, Hiroko, Ui, Hideaki, Shiomi, Kazuro, Yamaguchi, Yuuichi, Masuma, Rokuro, ...

Infections with parasitic helminths are important causes of morbidity and mortality worldwide. New drugs that are parasite specific and minimally toxic to the host are needed to counter these...

Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase)

Miyadera, Hiroko, Shiomi, Kazuro, Ui, Hideaki, Yamaguchi, Yuichi, Masuma, Rokuro, Tomoda, Hiroshi, ...

Enzymes in the mitochondrial respiratory chain are involved in various physiological events in addition to their essential role in the production of ATP by oxidative phosphorylation. The use of...

Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica

Okada, Mami, Huston, Christopher D., Mann, Barbara J., Petri, William A., Kita, Kiyoshi, Nozaki, Tomoyoshi

Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton...

PfPDE1, a novel cGMP-specific phosphodiesterase from the human malaria parasite Plasmodium falciparum

Yuasa, Keizo, Mi-Ichi, Fumika, Kobayashi, Tamaki, Yamanouchi, Masaya, Kotera, Jun, Kita, Kiyoshi, ...

This is the first report of molecular characterization of a novel cyclic nucleotide PDE (phosphodiesterase), isolated from the human malaria parasite Plasmodium falciparum and designated PfPDE1....

An evolutionary ‘intermediate state’ of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu

Arita, Masashi, Suematsu, Takuma, Osanai, Arihiro, Inaba, Takashi, Kamiya, Haruo, Kita, Kiyoshi, ...

EF-Tu delivers aminoacyl-tRNAs to ribosomes in the translation system. However, unusual truncations found in some animal mitochondrial tRNAs seem to prevent recognition by a canonical EF-Tu. We...

An anthelmintic compound, nafuredin, shows selective inhibition of complex I in helminth mitochondria

Ōmura, Satoshi, Miyadera, Hiroko, Ui, Hideaki, Shiomi, Kazuro, Yamaguchi, Yuuichi, Masuma, Rokuro, ...

Infections with parasitic helminths are important causes of morbidity and mortality worldwide. New drugs that are parasite specific and minimally toxic to the host are needed to counter these...

Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase)

Miyadera, Hiroko, Shiomi, Kazuro, Ui, Hideaki, Yamaguchi, Yuichi, Masuma, Rokuro, Tomoda, Hiroshi, ...

Enzymes in the mitochondrial respiratory chain are involved in various physiological events in addition to their essential role in the production of ATP by oxidative phosphorylation. The use of...

Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica

Okada, Mami, Huston, Christopher D., Mann, Barbara J., Petri, William A., Kita, Kiyoshi, Nozaki, Tomoyoshi

Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton...

PfPDE1, a novel cGMP-specific phosphodiesterase from the human malaria parasite Plasmodium falciparum

Yuasa, Keizo, Mi-Ichi, Fumika, Kobayashi, Tamaki, Yamanouchi, Masaya, Kotera, Jun, Kita, Kiyoshi, ...

This is the first report of molecular characterization of a novel cyclic nucleotide PDE (phosphodiesterase), isolated from the human malaria parasite Plasmodium falciparum and designated PfPDE1....

An evolutionary ‘intermediate state’ of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu

Arita, Masashi, Suematsu, Takuma, Osanai, Arihiro, Inaba, Takashi, Kamiya, Haruo, Kita, Kiyoshi, ...

EF-Tu delivers aminoacyl-tRNAs to ribosomes in the translation system. However, unusual truncations found in some animal mitochondrial tRNAs seem to prevent recognition by a canonical EF-Tu. We...

Overexpression of Peroxisome Proliferator-Activated Receptor γ Co-Activator-1α Leads to Muscle Atrophy with Depletion of ATP

Miura, Shinji, Tomitsuka, Eriko, Kamei, Yasutomi, Yamazaki, Tomomi, Kai, Yuko, Tamura, Mayumi, ...

Peroxisome proliferator-activated receptor-γ co-activator-1α (PGC-1α) is a key nuclear receptor co-activator for mitochondrial biogenesis. Here we report that overexpression of PGC-1α in skeletal...

Independent Evolution of Pyrimethamine Resistance in Plasmodium falciparum Isolates in Melanesia▿

Mita, Toshihiro, Tanabe, Kazuyuki, Takahashi, Nobuyuki, Tsukahara, Takahiro, Eto, Hideaki, Dysoley, Lek, ...

Pyrimethamine resistance in Plasmodium falciparum has previously been shown to have emerged once in Southeast Asia, from where it spread to Africa. Pyrimethamine resistance in this parasite is known...

Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate

Inaoka, Daniel Ken, Takashima, Eizo, Osanai, Arihiro, Shimizu, Hironari, Nara, Takeshi, Aoki, Takashi, ...

The Trypanosoma cruzi dihydroorotate dehydrogenase, a key enzyme in pyrimidine de novo biosynthesis and redox homeostasis, was crystallized in complex with its first reaction product, orotate.

Anaerobic NADH-Fumarate Reductase System Is Predominant in the Respiratory Chain of Echinococcus multilocularis, Providing a Novel Target for the Chemotherapy of Alveolar Echinococcosis▿

Matsumoto, Jun, Sakamoto, Kimitoshi, Shinjyo, Noriko, Kido, Yasutoshi, Yamamoto, Nao, Yagi, Kinpei, ...

Alveolar echinococcosis, which is due to the massive growth of larval Echinococcus multilocularis, is a life-threatening parasitic zoonosis distributed widely across the northern hemisphere....

Functional importance of Crenarchaea-specific extra-loop revealed by an X-ray structure of a heterotetrameric crenarchaeal splicing endonuclease

Yoshinari, Shigeo, Shiba, Tomoo, Inaoka, Daniel-Ken, Itoh, Takashi, Kurisu, Genji, Harada, Shigeharu, ...

Archaeal splicing endonucleases (EndAs) are currently classified into three groups. Two groups require a single subunit protein to form a homodimer or homotetramer. The third group requires two...