BABASAKI, Katsuhiko, TAKAO, Toshifumi, SHIMONISHI, Yasutsugu, KURAHASHI, Kiyoshi
Subtilosin A, a new antibiotic produced by Bacillus subtilis 168, was extracted from culture medium with n-butanol and purified to homogeneity by a combination of gel filtration and thin-layer...
KOMURA, Sadaaki, KURAHASHI, Kiyoshi
An enzyme fraction, which catalyzes the ATP-PP1 exchange reaction dependent on the three constituent amino acids of polymyxin E, was partially purified from crude extracts of Aerobacillus...
KOMURA, Sadaaki, KURAHASHI, Kiyoshi
A partially purified enzyme of Aerobacillus polyaerogenes, which produces polymyxin E, activates L-2,4-diaminobutyric acid and binds it as a thioester. The incubation of the enzyme preparation with...
KOMURA, Sadaaki, KURAHASHI, Kiyoshi
An L-2,4-diaminobutyric acid activating enzyme was found in crude extracts of Aerobacillus polyaerogenes, which produces polymyxin E1 and E2. The enzyme was partially purified by sonication of the...
AKASHI, Katsuhiko, KURAHASHI, Kiyoshi
Gramicidin A is an antibiotic peptide produced by Bacillus brevis ATCC 8185, which also produces tyrocidines. An attempt was made to establish a cell-free enzyme system for gramicidin A synthesis. An...
Carbohydrate Metabolism in Bacillus brevis ATCC 9999 (1977)
ASATANI, Masayoshi, KURAHASHI, Kiyoshi
Bacillus brevis ATCC 9999, which produces gramicidin S, was found to be unable to ferment various carbohydrates such as glucose, galactose, mannose, lactose, maltose, sucrose, and mannitol. This...
Biochemical Studies on Gramicidin S Non-producing Mutants of Bacillus brevis ATCC 9999 (1974)
KAMBE, Michiyo, IMAE, Yasuo, KURAHASHI, Kiyoshi
Mutants which could not produce gramicidin S were isolated from Bacillus brevis ATCC 9999 by UV irradiation or N-methyl-N'-nitro-N-nitrosoguanidine treatment. They were classified into three groups...
FUJIKAWA, Kazuo, SAKAMOTO, Yohko, KURAHASHI, Kiyoshi
1. Two complementary fractions of tyrocidine-synthesizing system, Components I and II, were purified from a crude extract of Bacillus brevis ATCC 8185 by means of ammonium sulfate fractionation,...
YAMADA, MICHIYUKI, KURAHASHI, KIYOSHI
Phenylalanine racemase of Bacillus brevis NAGANO which produces gramicidin S was purified to a nearly homogeneous state. The purification procedure involved ultracentrifugation, ammonium sulfate...
The Role of Two Complementary Fractions of Gramicidin S Synthesizing Enzyme System (1968)
ITOH, HAJIME, YAMADA, MICHIYUKI, TOMINO, SHIRO, KURAHASHI, KIYOSHI
YAMADA, MICHIYUKI, KURAHASHI, KIYOSHI
A phenylalanine racemase [EC 5. 1. 1 group] was found to be present in the cell-free extract of Bacillus brevis NAGANO, which produces gramicidin S. This enzyme was partially purified by...
KAMOGAWA, ATSUMI, KURAHASHI, KIYOSHI
A mutant strain of Escherichia coli K 12 which could not grow on glucose as a sole carbon source was found to have a defect in the transport of glucose. However, this inability of the cells to...
Purification and Properties of Uridine Diphosphoglucose 4-epimerase from Escherichia coli (1964)
IMAE, YASUO, MORIKAWA, NOBUKO, KURAHASHI, KIYOSHI
A method for purification of UDPglucose 4-epimerase from Escherichia coli was described. An overall purification of 80-fold above the crude extract was achieved. With the use of this partially...