Kiyozo Asada

Factors influencing efficiency of transient gene expression in the red macrophyte Porphyra yezoensis (2008)

Fukuda, Satoru, Mikami, Koji, Uji, Toshiki, Park, Eun-Jeong, Ohba, Toshiharu, Asada, Kiyozo, ...

The marine red alga Porphyra yezoensis has been proposed as a model plant for physiological and genetic studies in seaweeds because of its biological and economical importance. However, the progress...

Investigation of the Molecular Mechanism of ICAN, a Novel Gene Amplification Method (2007)

Uemori, Takashi, Mukai, Hiroyuki, Takeda, Osamu, Moriyama, Mariko, Sato, Yoshimi, Hokazono, Shigekazu, ...

Isothermal and Chimeric primer-initiated Amplification of Nucleic acids (ICAN) allows the amplification of target DNA under isothermal conditions at around 55°C using only a pair of...

Highly Efficient Isothermal DNA Amplification System Using Three Elements of 5'-DNA-RNA-3' Chimeric Primers, RNaseH and Strand-displacing DNA Polymerase (2007)

Mukai, Hiroyuki, Uemori, Takashi, Takeda, Osamu, Kobayashi, Eiji, Yamamoto, Junko, Nishiwaki, Kazue, ...

We developed an efficient method of isothermally amplifying DNA termed ICAN, Isothermal and Chimeric primer-initiated Amplification of Nucleic acids. This method allows the amplification of target...

The expression profile of microRNAs in mouse embryos (2006)

Mineno, Junichi, Okamoto, Sachiko, Ando, Tatsuya, Sato, Masahiro, Chono, Hideto, Izu, Hiroyuki, ...

MicroRNAs (miRNAs), which are non-coding RNAs 18–25 nt in length, regulate a variety of biological processes, including vertebrate development. To identify new species of miRNA and to...

Enhancement of Retroviral Gene Transduction on a Dish Coated with a Cocktail of Two Different Polypeptides: One Exhibiting Binding Activity toward Target Cells, and the Other toward Retroviral Vectors (1998)

Asada, Kiyozo, Uemori, Takashi, Ueno, Takashi, Hashino, Kimikazu, Koyama, Nobuto, Kawamura, Akira, ...

CH-296, a recombinant fragment of human fibronectin (FN) composed of the cell-binding domain (C-domain), heparin-binding domain II (H-domain), and CS1 site, enhances the retrovirus-mediated gene...

Sequence Analysis of the 16S-23S Spacer Region in rRNA Operons of the Mollicutes : Acholeplasma has tRNA Genes in the Spacer Region but Mycoplasma does not (Commemoration Issue Dedicated to Professor Mituru Takanami On the Occasion of His Retirement) (1993)

Asada, Kiyozo, Uemori, Takashi, Nakagawa, Tomoko, Kato, Ikunoshin, Shiragami, Naohiro, Harasawa, Ryo

Organization and nucleotide sequence of the DNA polymerase gene from the archaeon Pyrococcus furiosus (1993)

Uemori, Takashi, Ishino, Yoshizumi, Toh, Hiroyuki, Asada, Kiyozo, Kato, Ikunoshin

We cloned the gene encoding the thermostable DNA polymerase from the archaeon Pyrococcus furiosus. The DNA fragment of 2785 base pair (bp) containing the structural gene for DNA polymerase was...

Cloning of the DNA Polymerase Gene of Bacillus caldotenax and Characterization of the Gene Product (1993)

Uemori, Takashi, Ishino, Yoshizumi, Fujita, Kayo, Asada, Kiyozo, Kato, Ikunoshin

The pol gene of the thermophilic eubacterium Bacillus caldotenax was cloned in a plasmid and expressed in Escherichia colL The PCR method was used to clone the gene with no previous knowledge of the...

The use of purine-rich oligonucleotides in triplex-mediated DNA isolation and generation of unidirectional deletions (1992)

Takabatake, Takashi, Asada, Kiyozo, Uchimura, Yuka, Ohdate, Miki, Kusukawa, Noriko

The Primary Structure of Porcine Liver Acylamino Acid-Releasing Enzyme Deduced from cDNA Sequences (1989)

Mitta, Masanori, Asada, Kiyozo, Uchimura, Yuka, Kimizuka, Fusao, Kato, Ikunoshin, Sakiyama, Fumio, ...

Two overlapping cloned cDNAs encoding the entire amino acid sequences of the subunits of acylamino acid-releasing enzyme (AARE) [EC 3.4.19.1] have been isolated from porcine liver cDNA λgtl0 cDNA...

Structure of replication origin of the Escherichia coli K-12 chromosome: the presence of spacer sequences in the orl region carrying information for autonomous replication (1982)

Asada, Kiyozo, Sugimoto, Kazunori, Oka, Atsuhiro, Takanami, Mituru, Hirota, Yukinori

The replication origin region of the Escherichia coli K-12 chromosome has been cloned, and a region of 245 base-pairs has been shown to contain all the information for autonomous replication (defined...

The expression profile of microRNAs in mouse embryos

Mineno, Junichi, Okamoto, Sachiko, Ando, Tatsuya, Sato, Masahiro, Chono, Hideto, Izu, Hiroyuki, ...

MicroRNAs (miRNAs), which are non-coding RNAs 18–25 nt in length, regulate a variety of biological processes, including vertebrate development. To identify new species of miRNA and to...

The expression profile of microRNAs in mouse embryos

Mineno, Junichi, Okamoto, Sachiko, Ando, Tatsuya, Sato, Masahiro, Chono, Hideto, Izu, Hiroyuki, ...

MicroRNAs (miRNAs), which are non-coding RNAs 18–25 nt in length, regulate a variety of biological processes, including vertebrate development. To identify new species of miRNA and to...