Parret, Annabel, Schubbert, Suzanne, ...
Costello syndrome is a mental retardation syndrome characterized by high birth weight, postnatal growth retardation, coarse face, loose skin, cardiovascular problems, and tumor predisposition. De...
The C2 domain of SynGAP is essential for stimulation of the Rap GTPase reaction (2008)
Pena, Vladimir, Hothorn, Michael, Eberth, Alexander, Kaschau, Nikolai, Parret, Annabel, Gremer, Lothar, ...
Structural insights into the Cyclin T1-Tat-TAR RNA transcription activation complex from EIAV (2008)
Anand, Kanchan, Schulte, Antje, Vogel-Bachmayr, Karin, Scheffzek, Klaus, Geyer, Matthias
Thioredoxin as a fusion tag for carrier-driven crystallization (2008)
Corsini, Lorenzo, Hothorn, Michael, Scheffzek, Klaus, Sattler, Michael, Stier, Gunter
Structural investigations are frequently hindered by difficulties in obtaining diffracting crystals of the target protein. Here, we report the crystallization and structure solution of the U2AF...
Andrea Friebel, Alfred Wittinghofer, Klaus Scheffzek, Max Von
The bacterial enteropathogen ######### # ########### employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family...
Reynaud, Emmanuel G, Andrade, Miguel A, Bonneau, Fabien, Ly, Thi, Knop, Michael, Scheffzek, Klaus, ...
Abstract Background Compartmentalization is a key feature of eukaryotic cells, but its evolution remains poorly understood. GTPases are the oldest enzymes that use nucleotides as substrates and they...
with the evolution of compartmentalization (2005)
Emmanuel G Reynaud, Miguel A Andrade, Fabien Bonneau, Thi Bach, Nga Ly, Michael Knop, ...
Human Lsg1 defines a family of essential GTPases that correlates
Amino acids of the bacterial toxin SopE involved in G nucleotide exchange on Cdc42 (2003)
Schlumberger,Markus C., Friebel,Andrea, Buchwald,Gretel, Scheffzek,Klaus, Wittinghofer,Alfred, Hardt,Wolf-Dietrich
Structural fingerprints of the Ras-GTPase activating proteins neurofibromin and p120GAP (2003)
Ahmadian,Mohammad Reza, Kiel,Christina, Stege,Patricia, Scheffzek,Klaus
Ras specific GTPase activating proteins (GAPs), neurofibromin and p120GAP, bind GTP bound Ras and efficiently complement its active site. Here we present comparative data from mutations and...
Structural fingerprints of the Ras-GTPase activating proteins neurofibromin and p120GAP (2003)
Ahmadian, Mohammad Reza, Kiel, Christina, Stege, Patricia, Scheffzek, Klaus
Ras specific GTPase activating proteins (GAPs), neurofibromin and p120GAP, bind GTP bound Ras and efficiently complement its active site. Here we present comparative data from mutations and...
Amino acids of the bacterial toxin SopE involved in G nucleotide exchange on Cdc42 (2003)
Schlumberger, Markus C., Friebel, Andrea, Buchwald, Gretel, Scheffzek, Klaus, Wittinghofer, Alfred, Hardt, Wolf-Dietrich
Stumber,Michael, Geyer,Matthias, Graf,Robert, Kalbitzer,Hans Robert, Scheffzek,Klaus, Haeberlen,Ulrich
The folding, structure and biological function of many proteins are inherently dynamic properties of the protein molecule. Often, the respective molecular processes are preserved upon protein...
Stumber,Michael, Geyer,Matthias, Graf,Robert, Kalbitzer,Hans Robert, Scheffzek,Klaus, Haeberlen,Ulrich
The folding, structure and biological function of many proteins are inherently dynamic properties of the protein molecule. Often, the respective molecular processes are preserved upon protein...
Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE (2002)
Buchwald,Gretel, Friebel,Andrea, Galán,Jorge E., Hardt,Wolf-Dietrich, Wittinghofer,Alfred, Scheffzek,Klaus
The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...
Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE (2002)
Buchwald, Gretel, Friebel, Andrea, Galán, Jorge E., Hardt, Wolf-Dietrich, Wittinghofer, Alfred, Scheffzek, Klaus
The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...
Stumber, Michael, Geyer, Matthias, Graf, Robert, Kalbitzer, Hans Robert, Scheffzek, Klaus, Haeberlen, Ulrich
The folding, structure and biological function of many proteins are inherently dynamic properties of the protein molecule. Often, the respective molecular processes are preserved upon protein...
Stumber, Michael, Geyer, Matthias, Graf, Robert, Kalbitzer, Hans Robert, Scheffzek, Klaus, Haeberlen, Ulrich
The folding, structure and biological function of many proteins are inherently dynamic properties of the protein molecule. Often, the respective molecular processes are preserved upon protein...
The Ras-Byr2RBD Complex: Structural Basis for Ras Effector Recognition in Yeast (2001)
Scheffzek,Klaus, Grünewald,Petra, Wohlgemuth,Sabine, Kabsch,Wolfgang, Tu,Hua, Wigler,Mike, ...
The small GTP binding protein Ras has important roles in cellular growth and differentiation. Mutant Ras is permanently active and contributes to cancer development. In its activated form, Ras...
The Ras-Byr2RBD Complex: Structural Basis for Ras Effector Recognition in Yeast (2001)
Scheffzek, Klaus, Grünewald, Petra, Wohlgemuth, Sabine, Kabsch, Wolfgang, Tu, Hua, Wigler, Mike, ...
The small GTP binding protein Ras has important roles in cellular growth and differentiation. Mutant Ras is permanently active and contributes to cancer development. In its activated form, Ras...
The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI (2000)
Scheffzek,Klaus, Stephan,Ilona, Jensen,Ole N., Illenberger,Daria, Gierschik,Peter
Rho family-specific guanine nucleotide dissociation inhibitors (RhoGDIs) decrease the rate of nucleotide dissociation and release Rho proteins such as RhoA, Rac and Cdc42 from membranes, forming...
The Rac-RhoGDI complex and the structural basis for the regulation of Rho proteins by RhoGDI (2000)
Scheffzek, Klaus, Stephan, Ilona, Jensen, Ole N., Illenberger, Daria, Gierschik, Peter
Rho family-specific guanine nucleotide dissociation inhibitors (RhoGDIs) decrease the rate of nucleotide dissociation and release Rho proteins such as RhoA, Rac and Cdc42 from membranes, forming...
Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)
Ahmadian,Mohammad Reza, Zor,Tsaffrir, Vogt,Dorothee, Kabsch,Wolfgang, Selinger,Zvi, Wittinghofer,Alfred, ...
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...
Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)
Ahmadian,Mohammad Reza, Zor,Tsaffrir, Vogt,Dorothee, Kabsch,Wolfgang, Selinger,Zvi, Wittinghofer,Alfred, ...
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...
Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)
Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...
Guanosine triphosphatase stimulation of oncogenic Ras mutants (1999)
Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...
Structural analysis of the GAP-related domain from neurofibromin and its implications (1998)
Scheffzek,Klaus, Ahmadian,Mohammad Reza, Wiesmüller,Lisa, Kabsch,Wolfgang, Stege,Patricia, Schmitz,Frank, ...
Neurofibromin is the product of the NF1 gene, whose alteration is responsible for the pathogenesis of neurofibromatosis type 1 (NF1), one of the most frequent genetic disorders in man. It acts as a...
GTPase-activating proteins: helping hands to complement an active site (1998)
Scheffzek,Klaus, Ahmadian,Mohammad Reza, Wittinghofer,Alfred
Structural analysis of the GAP-related domain from neurofibromin and its implications (1998)
Scheffzek, Klaus, Ahmadian, Mohammad Reza, Wiesmüller, Lisa, Kabsch, Wolfgang, Stege, Patricia, Schmitz, Frank, ...
Neurofibromin is the product of the NF1 gene, whose alteration is responsible for the pathogenesis of neurofibromatosis type 1 (NF1), one of the most frequent genetic disorders in man. It acts as a...
GTPase-activating proteins: helping hands to complement an active site (1998)
Scheffzek, Klaus, Ahmadian, Mohammad Reza, Wittinghofer, Alfred
Scheffzek,Klaus, Ahmadian,Mohammad Reza, Kabsch,Wolfgang, Wiesmüller,Lisa, Lautwein,A., Schmitz,Frank, ...
Scheffzek, Klaus, Ahmadian, Mohammad Reza, Kabsch, Wolfgang, Wiesmüller, Lisa, Lautwein, A., Schmitz, Frank, ...
Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form (1995)
Scheffzek,Klaus, Klebe,Christian, Fritz-Wolf,Karin, Kabsch,Wolfgang, Wittinghofer,Alfred
The Ran proteins constitute a distinct branch of the superfamily of Ras-related GTP-binding proteins which function as molecular switches cycling between GTP-bound on and GDP-bound off states. Ran is...
Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form (1995)
Scheffzek, Klaus, Klebe, Christian, Fritz-Wolf, Karin, Kabsch, Wolfgang, Wittinghofer, Alfred
The Ran proteins constitute a distinct branch of the superfamily of Ras-related GTP-binding proteins which function as molecular switches cycling between GTP-bound on and GDP-bound off states. Ran is...
Heidelberg, Univ., Diss., 1995.
Guanosine triphosphatase stimulation of oncogenic Ras mutants
Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...
Conformational Switch and Role of Phosphorylation in PAK Activation
Buchwald, Gretel, Hostinova, Eva, Rudolph, Markus G., Kraemer, Astrid, Sickmann, Albert, Meyer, Helmut E., ...
p21-activated protein kinases (PAKs) are involved in signal transduction processes initiating a variety of biological responses. They become activated by interaction with Rho-type small GTP-binding...
Márquez, José Antonio, Hasenbein, Sonja, Koch, Brigitte, Fieulaine, Sonia, Nessler, Sylvie, Russell, Robert B., ...
The histidine containing phospho carrier protein (HPr) kinase/phosphatase is involved in carbon catabolite repression, mainly in Gram-positive bacteria. It is a bifunctional enzyme that...
Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE
Buchwald, Gretel, Friebel, Andrea, Galán, Jorge E., Hardt, Wolf-Dietrich, Wittinghofer, Alfred, Scheffzek, Klaus
The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...
Mijakovic, Ivan, Poncet, Sandrine, Galinier, Anne, Monedero, Vicente, Fieulaine, Sonia, Janin, Joël, ...
In most Gram-positive bacteria, serine-46-phosphorylated HPr (P-Ser-HPr) controls the expression of numerous catabolic genes (≈10% of their genome) by acting as catabolite corepressor. HPr...
Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering
Márquez, José A., Smith, C.I.Edvard, Petoukhov, Maxim V., Lo Surdo, Paola, Mattsson, Pekka T., Knekt, Marika, ...
Brutons’s tyrosine kinase (Btk) is a non-receptor protein tyrosine kinase (nrPTK) essential for the development of B lymphocytes in humans and mice. Like Src and Abl PTKs, Btk contains a conserved...
Hothorn, Michael, Wolf, Sebastian, Aloy, Patrick, Greiner, Steffen, Scheffzek, Klaus
Pectin methylesterase (PME) and invertase are key enzymes in plant carbohydrate metabolism. Inhibitors of both enzymes constitute a sequence family of extracellular proteins. Members of this family...
Reynaud, Emmanuel G, Andrade, Miguel A, Bonneau, Fabien, Ly, Thi Bach Nga, Knop, Michael, Scheffzek, Klaus, ...
Guanosine triphosphatase stimulation of oncogenic Ras mutants
Ahmadian, Mohammad Reza, Zor, Tsaffrir, Vogt, Dorothee, Kabsch, Wolfgang, Selinger, Zvi, Wittinghofer, Alfred, ...
Interest in the guanosine triphosphatase (GTPase) reaction of Ras as a molecular drug target stems from the observation that, in a large number of human tumors, Ras is characteristically mutated at...
Conformational Switch and Role of Phosphorylation in PAK Activation
Buchwald, Gretel, Hostinova, Eva, Rudolph, Markus G., Kraemer, Astrid, Sickmann, Albert, Meyer, Helmut E., ...
p21-activated protein kinases (PAKs) are involved in signal transduction processes initiating a variety of biological responses. They become activated by interaction with Rho-type small GTP-binding...
Márquez, José Antonio, Hasenbein, Sonja, Koch, Brigitte, Fieulaine, Sonia, Nessler, Sylvie, Russell, Robert B., ...
The histidine containing phospho carrier protein (HPr) kinase/phosphatase is involved in carbon catabolite repression, mainly in Gram-positive bacteria. It is a bifunctional enzyme that...
Structural basis for the reversible activation of a Rho protein by the bacterial toxin SopE
Buchwald, Gretel, Friebel, Andrea, Galán, Jorge E., Hardt, Wolf-Dietrich, Wittinghofer, Alfred, Scheffzek, Klaus
The bacterial enteropathogen Salmonella typhimurium employs a type III secretion system to inject bacterial toxins into the host cell cytosol. These toxins transiently activate Rho family GTP-binding...
Mijakovic, Ivan, Poncet, Sandrine, Galinier, Anne, Monedero, Vicente, Fieulaine, Sonia, Janin, Joël, ...
In most Gram-positive bacteria, serine-46-phosphorylated HPr (P-Ser-HPr) controls the expression of numerous catabolic genes (≈10% of their genome) by acting as catabolite corepressor. HPr...
Conformation of full-length Bruton tyrosine kinase (Btk) from synchrotron X-ray solution scattering
Márquez, José A., Smith, C.I.Edvard, Petoukhov, Maxim V., Lo Surdo, Paola, Mattsson, Pekka T., Knekt, Marika, ...
Brutons’s tyrosine kinase (Btk) is a non-receptor protein tyrosine kinase (nrPTK) essential for the development of B lymphocytes in humans and mice. Like Src and Abl PTKs, Btk contains a conserved...
Hothorn, Michael, Wolf, Sebastian, Aloy, Patrick, Greiner, Steffen, Scheffzek, Klaus
Pectin methylesterase (PME) and invertase are key enzymes in plant carbohydrate metabolism. Inhibitors of both enzymes constitute a sequence family of extracellular proteins. Members of this family...
Reynaud, Emmanuel G, Andrade, Miguel A, Bonneau, Fabien, Ly, Thi Bach Nga, Knop, Michael, Scheffzek, Klaus, ...
A novel bipartite phospholipid-binding module in the neurofibromatosis type 1 protein
D'angelo, Igor, Welti, Stefan, Bonneau, Fabien, Scheffzek, Klaus
Neurofibromatosis type 1 (NF1) is a common tumour predisposition syndrome associated with numerous clinical complications. Mutations in the tumour suppressor gene NF1 are responsible for disease...
The FACT Spt16 “peptidase” domain is a histone H3–H4 binding module
Stuwe, Tobias, Hothorn, Michael, Lejeune, Erwan, Rybin, Vladimir, Bortfeld, Miriam, Scheffzek, Klaus, ...
The FACT complex is a conserved cofactor for RNA polymerase II elongation through nucleosomes. FACT bears histone chaperone activity and contributes to chromatin integrity. However, the molecular...
The C2 domain of SynGAP is essential for stimulation of the Rap GTPase reaction
Pena, Vladimir, Hothorn, Michael, Eberth, Alexander, Kaschau, Nikolai, Parret, Annabel, Gremer, Lothar, ...
The brain-specific synaptic guanosine triphosphatase (GTPase)-activating protein (SynGAP) is important in synaptic plasticity. It shows dual specificity for the small guanine nucleotide-binding...