Kohji Hizume

Removal of histone tails from nucleosome dissects the physical mechanisms of salt-induced aggregation, linker histone H1-induced compaction, and 30-nm fiber formation of the nucleosome array (2009)

Hizume, Kohji, Nakai, Tonau, Araki, Sumiko, Prieto, Eloise, Yoshikawa, Kenichi, Takeyasu, Kunio

In order to reveal the roles of histone tails in the formation of higher-order chromatin structures, we employed atomic force microscopy (AFM), and an in vitro reconstitution system to examine the...

Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner (2007)

Hizume, Kohji, Araki, Sumiko, Yoshikawa, Kenichi, Takeyasu, Kunio

TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro...

Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner (2007)

Hizume, Kohji, Araki, Sumiko, Yosihkawa, Kenichi, Takeyasu, Kunio

TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro...

Transcriptional Coactivator PC4, a Chromatin-associated Protein, induces Chromatin Condensation (2006)

Das, Chandrima, Hizume, Kohji, Batta, Kiran, Kumar, Prashanth BR, Gadad, Shrikanth S, Ganguly, Semanti, ...

Human transcriptional coactivator PC4 is a highly abundant multifunctional protein, which plays diverse important roles in cellular processes including transcription, replication and repair. It is...

Protective effect of vitamin C against double-strand breaks in reconstituted chromatin visualized by single-molecule observation (2006)

Yoshikawa, Yuko, Hizume, Kohji, Oda, Yoshiko, Takeyasu, Kunio, Araki, Sumiko, Yoshikawa, Kenichi

Direct attack to genomic DNA by reactive oxygen species causes various types of lesions, including base modi. cations and strand breaks. The most significant lesion is considered to be an unrepaired...

Hydrodynamic radius of circular DNA is larger than that of linear DNA (2006)

Araki, Sumiko, Nakai, Tonau, Hizume, Kohji, Takeyasu, Kunio, Yoshikawa, Kenichi

It is generally accepted that linear polymers are larger, such as with regard to the end-to-end distance and gyration radius, than the corresponding circular polymers. We measured the Brownian motion...

Transcriptional Coactivator PC4, a Chromatin-associated Protein, induces Chromatin Condensation (2006)

Das, Chandrima, Hizume, Kohji, Batta, Kiran, Kumar, Prashanth BR, Gadad, Shrikanth S, Ganguly, Semanti, ...

Human transcriptional coactivator PC4 is a highly abundant multifunctional protein, which plays diverse important roles in cellular processes including transcription, replication and repair. It is...

Comparative structural biology of the genome: nano-scale imaging of single nucleus from different kingdoms reveals the common physicochemical property of chromatin with a 40 nm structural unit (2006)

Kobori, Toshiro, Kodama, Mami, Hizume, Kohji, Yoshimura, Shige H., Ohtani, Toshio, Takeyasu, Kunio

Genome function is closely linked to the higher-order chromatin structures. To reveal a structural basis for the interphase chromatin organization, the ‘on-substrate’ lysis procedure was applied...

Comparative structural biology of the genome: nano-scale imaging of single nucleus from different kingdoms reveals the common physicochemical property of chromatin with a 40 nm structural unit (2006)

Kobori, Toshiro, Kodama, Mami, Hizume, Kohji, Yoshimura, Shige H., Ohtani, Toshio, Takeyasu, Kunio

Genome function is closely linked to the higher-order chromatin structures. To reveal a structural basis for the interphase chromatin organization, the ‘on-substrate’ lysis procedure was applied...

Comparative structural biology of the genome: nano-scale imaging of single nucleus from different kingdoms reveals the common physicochemical property of chromatin with a 40 nm structural unit (2006)

Kobori, Toshiro, Kodama, Mami, Hizume, Kohji, Yoshimura, Shige H., Ohtani, Toshio, Takeyasu, Kunio

Genome function is closely linked to the higher-order chromatin structures. To reveal a structural basis for the interphase chromatin organization, the ‘on-substrate’ lysis procedure was applied...

Phase Transition in Reconstituted Chromatin (2004)

Nakai, Tonau, Hizume, Kohji, Yoshimura, Shige. H., Takeyasu, Kunio, Yoshikawa, Kenichi

By observing reconstituted chromatin by fluorescence microscopy (FM) and atomic force microscopy (AFM), we found that the density of nucleosomes exhibits a bimodal profile, i.e., there is a large...

Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy (2004)

Kim, Joongbaek, Yoshimura, Shige H., Hizume, Kohji, Ohniwa, Ryosuke L., Ishihama, Akira, Takeyasu, Kunio

A small container of several to a few hundred µm3 (i.e. bacterial cells and eukaryotic nuclei) contains extremely long genomic DNA (i.e. mm and m long, respectively) in a highly organized fashion....

Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein–protein assembly

Sakai, Akiko, Hizume, Kohji, Sutani, Takashi, Takeyasu, Kunio, Yanagida, Mitsuhiro

Condensin and cohesin are chromosomal protein complexes required for chromosome condensation and sister chromatid cohesion, respectively. They commonly contain the SMC (structural maintenance of...

Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy

Kim, Joongbaek, Yoshimura, Shige H., Hizume, Kohji, Ohniwa, Ryosuke L., Ishihama, Akira, Takeyasu, Kunio

A small container of several to a few hundred µm3 (i.e. bacterial cells and eukaryotic nuclei) contains extremely long genomic DNA (i.e. mm and m long, respectively) in a highly organized fashion....

Condensin but not cohesin SMC heterodimer induces DNA reannealing through protein–protein assembly

Sakai, Akiko, Hizume, Kohji, Sutani, Takashi, Takeyasu, Kunio, Yanagida, Mitsuhiro

Condensin and cohesin are chromosomal protein complexes required for chromosome condensation and sister chromatid cohesion, respectively. They commonly contain the SMC (structural maintenance of...

Fundamental structural units of the Escherichia coli nucleoid revealed by atomic force microscopy

Kim, Joongbaek, Yoshimura, Shige H., Hizume, Kohji, Ohniwa, Ryosuke L., Ishihama, Akira, Takeyasu, Kunio

A small container of several to a few hundred µm3 (i.e. bacterial cells and eukaryotic nuclei) contains extremely long genomic DNA (i.e. mm and m long, respectively) in a highly organized fashion....

Protective Effect of Vitamin C against Double-Strand Breaks in Reconstituted Chromatin Visualized by Single-Molecule Observation

Yoshikawa, Yuko, Hizume, Kohji, Oda, Yoshiko, Takeyasu, Kunio, Araki, Sumiko, Yoshikawa, Kenichi

Direct attack to genomic DNA by reactive oxygen species causes various types of lesions, including base modifications and strand breaks. The most significant lesion is considered to be an unrepaired...

Transcriptional Coactivator PC4, a Chromatin-Associated Protein, Induces Chromatin Condensation▿ †

Das, Chandrima, Hizume, Kohji, Batta, Kiran, Kumar, B. R. Prashanth, Gadad, Shrikanth S., Ganguly, Semanti, ...

Human transcriptional coactivator PC4 is a highly abundant multifunctional protein which plays diverse important roles in cellular processes, including transcription, replication, and repair. It is...

Topoisomerase II, scaffold component, promotes chromatin compaction in vitro in a linker-histone H1-dependent manner

Hizume, Kohji, Araki, Sumiko, Yoshikawa, Kenichi, Takeyasu, Kunio

TopoisomeraseII (Topo II) is a major component of chromosomal scaffolds and essential for mitotic chromosome condensation, but the mechanism of this action remains unknown. Here, we used an in vitro...