REVIEW ARTICLE Structure and physiological function of calpains (2008)
Hiroyuki Sorimachi, Shoichi Ishiura, Koichi Suzuki
For a long time now, two ubiquitously expressed mammalian calpain isoenzymes have been used to explore the structure and function of calpain. Although these two calpains, µ- and mcalpains, still...
Kageyama, Shinji, Shinmura, Kazuya, Yamamoto, Hiroko, Goto, Masanori, Suzuki, Koichi, Tanioka, Fumihiko, ...
The PCR-based DNA fingerprinting method called the methylation-sensitive amplified fragment length polymorphism (MS-AFLP) analysis is used for genome-wide scanning of methylation status. In this...
Expression of retinoic acid-inducible gene-I in lupus nephritis (2007)
Suzuki, Koichi, Imaizumi, Tadaatsu, Tsugawa, Koji, Ito, Etsuro, Tanaka, Hiroshi
Konishi, Yukiko, Suzuki, Koichi, Wada, Hidetoshi, Watanabe, Hiroshi, Ogura, Hiroyuki, Sugamori, Yuno, ...
Abstract Background Recently, the right gastroepiploic artery (RGEA) has been used in coronary artery bypass grafting (CABG) as an alternative arterial graft. Unfortunately, an increased incidence of...
Hata, Shoji, Koyama, Suguru, Kawahara, Hiroyuki, Doi, Naoko, Maeda, Tatsuya, Toyama-Sorimachi, Noriko, ...
Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in...
Hata, Shoji, Koyama, Suguru, Kawahara, Hiroyuki, Doi, Naoko, Maeda, Tatsuya, Toyama-Sorimachi, Noriko, ...
Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in...
Hata, Shoji, Koyama, Suguru, Kawahara, Hiroyuki, Doi, Naoko, Maeda, Tatsuya, Toyama-Sorimachi, Noriko, ...
Calpain is a Ca2+-regulated cytosolic protease. Mammals have 14 calpain genes, half of which are predominantly expressed in specific organ(s); the rest are expressed ubiquitously. A defect in...
Noriyuki Ojima, Shin-ichi Ikeda, Osamu Koike, Kosuke Fujita, Koichi Suzuki
The leaf beetle, Gastrophysa atrocyanea Motschulsky (Coleoptera: Chrysomelidae) has been regarded as a bioagent suitable for the extermination of certain weeds, especially Rumex obtusifolius. We...
Retention of pendrin in the endoplasmic reticulum is a major mechanism for Pendred syndrome (2002)
Rotman-Pikielny, Pnina, Hirschberg, Koret, Maruvada, Padma, Suzuki, Koichi, Royaux, Ines E., Green, Eric D., ...
Pendred syndrome is a major cause of congenital deafness, goiter and defective iodide organification. Mutations in the transmembrane protein, pendrin, cause diminished export of iodide from thyroid...
Coexpression of the CUG-Binding Protein Reduces DM Protein Kinase Expression in COS Cells (2001)
Takahashi, Nobuhiro, Sasagawa, Noboru, Usuki, Fusako, Kino, Yosbihiro, Kawahara, Hiroyuki, Sorimachi, Hiroyuki, ...
Myotonic dystrophy (DM) is the most common form of adult onset muscular dystrophy. Patients have a large CTG repeat expansion in the 3' untranslated region of the DMPK gene, which encodes DM protein...
Nakagawa, Kazuhiro, Masumoto, Hajime, Sorimachi, Hiroyuki, Suzuki, Koichi
Calpain is a heterodimeric, intracellular Ca2+-dependent, “bio-modulator” that alters the properties of substrates through site-specific proteolysis. It has been proposed that calpains are...
Limited Proteolysis of Filamin Is Catalyzed by Caspase-3 in U937 and Jurkat Cells (2001)
Umeda, Tatsuya, Kouchi, Zen, Kawahara, Hiroyuki, Tomioka, Shigeo, Sasagawa, Noboru, Maeda, Tatsuya, ...
Members of the caspase family have been implicated as key mediators of apoptosis in mammalian cells. However, few of their substrates are known to have physiological significance in the apoptotic...
The Structure of Calpain (2001)
Sorimachi, Hiroyuki, Suzuki, Koichi
Recent very rapid developments in genome and EST projects have identified an increasing number of gene products homologous to those that were previously identified by other methods. Calpain is no...
Tagawa, Kazuhiko, Taya, Choji, Hayashi, Yukiko, Nakagawa, Masahiro, Ono, Yasuko, Fukuda, Rie, ...
A defect of the gene for p94 (calpain 3), a skeletal muscle-specific calpain, is responsible for limb girdle muscular dystrophy type 2A (LGMD2A), or ‘calpainopathy’, which is an autosomal...
Koike, Hisashi, Seki, Hiroaki, Kouchi, Zen, Ito, Masayuki, Kinouchi, Tadatoshi, Tomioka, Shigeo, ...
We developed an assay method using a novel quenched fluorescent substrate (QFS) flanking the β-cleavage site of amyloid precursor protein (APP), and purified a candidate β-secre-tase from bovine...
Masumoto, Hajime, Yoshizawa, Toshio, Sorimachi, Hiroyuki, Nishino, Takeshi, Ishiura, Shoichi, Suzuki, Koichi
Recombinant human m-calpain was produced in a soluble form at a level of 20 mg/liter of Sf-9 cell culture by the coexpression of recombinant human m-calpain large (m80K) and small (30K) subunits...
Amano, Susumu, Kawasaki, Hiroshi, Ishiura, Shoichi, Kawashima, Seiichi, Suzuki, Koichi, Emori, Yasufumi
Drosophila calpain (Dm-calpain) produced in Escherichia coli has a distinct Ca2+-depen-dent activity. By using a recombinant Dm-calpain, we searched for its substrates occurring in Drosophila...
Ono, Yasuko, Kinouchi, Tadatoshi, Sorimachi, Hiroyuki, Ishiura, Shoichi, Suzuki, Koichi
Alzheimer's disease amyloid precursor protein (APP) generates a β-amyloid protein (Aβ) that is a main component of the senile plaques found in the brains of Alzheimer's disease patients. APP is...
Calpain-Calpastatin Interactions in Epidermoid Carcinoma KB Cells (1994)
Nagao, Shunsuke, Saido, Takaomi C., Akita, Yoshiko, Tsuchiya, Takahide, Suzuki, Koichi, Kawashima, Seiichi
We examined the activation of μ-calpain in human epidermoid carcinoma KB cells following a rise in intracellular calcium concentration using antibodies specifically recognizing different activation...
Phosphatidylserine Specific Binding Protein in Rat Brain: Purification and Characterization (1993)
Nakaoka, Takashi, Kojima, Naoya, Hamamoto, Toshiro, Kurosawa, Nobuyuki, Lee, Young Choon, Kawasaki, Hiroshi, ...
A calcium-independent phosphatidylserine specific binding protein detected on liposome blotting analysis was purified from rat brain and revealed to be identical to myristoylated, alanine-rich C...
Saido, Takaomi C., Nagao, Shunsuke, Shiramine, Mikiro, Tsukaguchi, Maya, Sorimachi, Hiroyuki, Murofushi, Hiromu, ...
A novel method to observe the autolytic activation of a mammalian cytoplasmic calcium protease, μ-calpain, was developed using a set of antipeptidic antibodies capable of distinguishing between the...
Kozutsumi, Yasunori, Kawano, Takehiro, Kawasaki, Hiroshi, Suzuki, Koichi, Yamakawa, Tamio, Suzuki, Akemi
The hydroxylation of CMP-N-acetylneuraminic acid (CMP-NeuAc) in the formation of CMP-N-glycolylneuraminic acid requires several components which comprise an electron transport system. A protein,...
Sorimachi, Hiroyuki, Emori, Yasufumi, Kawasaki, Hiroshi, Suzuki, Koichi, Inoue, Yasuo
Recently we have cloned the cDNAs and genomic DNAs for apopolysialoglycoproteins (apoPSGPs) of Salmo gairdneri (rainbow trout) [Sorimachi, H., Emori, Y., Kawasaki, H., Kitajima, K., Inoue, S.,...
Enzymatic Properties of a Novel Phorbol Ester Receptor/Protein Kinase, nPKC (1989)
Konno, Yasuhiko, Ohno, Shigeo, Akita, Yoshiko, Kawasaki, Hiroshi, Suzuki, Koichi
A protein kinase C-related cDNA encodes a novel phorbol ester receptor/protein kinase, nPKCϵ, clearly distinct from the four “conventional” PKCs [Ohno, S., Akita, Y., Konno, Y., Imajoh,...
Kawasaki, Hiroshi, Emori, Yasufumi, Imajoh-Ohmi, Shinobu, Minami, Yasufumi, Suzuki, Koichi
We reported previously the cDNA cloning of the endogenous inhibitor for calcium-dependent protease (CANP inhibitor, calpastatin) and the expression of its fragments in Escherichia coli. The CANP...
Minami, Yasufumi, Emori, Yasufumi, Imajoh-Ohmi, Shinobu, Kawasaki, Hiroshi, Suzuki, Koichi
A mutant of the small subunit of rabbit calcium-dependent protease lacking the amino-terminal one-fourth produced in Escherichia coli could associate with the native large subunit to exert protease...
E-F Hand Structure-Domain of Calcium-Activated Neutral Protease (CANP) Can Bind Ca2+ Ions (1987)
MINAMI, Yasufumi, EMORI, Yasufumi, KAWASAKI, Hiroshi, SUZUKI, Koichi
The cDNA fragments corresponding to the domains with four consecutive E-F hand structures in the large and small subunits of chicken and rabbit calcium-activated neutral protease (CANP) were inserted...
Further Characterization and Structural Studies on Human Placenta Lectin (1987)
HIRABAYASHI, Jun, KAWASAKI, Hiroshi, SUZUKI, Koichi, KASAI, Ken-ichi
The properties of a previously purified β-galactoside-binding lectin of human pla-centa were studied in detail. Isoelectric focusing gave multiple bands around pH 4.9, although the lectin...
IMAJOH, Shinobu, KAWASAKI, Hiroshi, SUZUKI, Koichi
High-Ca2+-requiring calcium-activated neutral protease (mCANP), a dimeric enzyme composed of large (Mr = 80,000) and small (Mr = 28,000) subunits, is resistant to carboxypeptidase...
Affinity Labeling of the Allosteric Site of Fructose 1,6-Bisphosphatase with an AMP Analog (1987)
SAKAI, Keiko, KAWASHIMA, Seiichi, SUZUKI, Koichi, IMAHORI, Kazutomo
D-Fructose 1, 6-bisphosphatase [EC 3.1.3.11, FBPase] is one of the key enzymes in glyconeogenesis and its activity is controlled by various effectors such as substrate, AMP and ATP. To analyze this...
KAWASAKI, Hiroshi, IMAJOH, Shinobu, SUZUKI, Koichi
The microscale separation of peptides based on the difference in positive charge was examined with tryptic digests of apomyoglobin and calmodulin. By this separation method, C-terminal and blocked...
Complete Amino Acid Sequence of 14 kDa {beta}-Galactoside-Binding Lectin of Chick Embryo (1987)
HIRABAYASHI, Jun, KAWASAKI, Hiroshi, SUZUKI, Koichi, KASAI, Ken-ichi
The complete amino acid sequence of a soluble β-galactoside-binding lectin (subunit MW 14,500) of chick embryo was determined. The protein consists of 134 amino acids beginning with serine and...
KAWASAKI, Hiroshi, IMAJOH, Shinobu, KAWASHIMA, Seiichi, HAYASHI, Hiroaki, SUZUKI, Koichi
The small subunits of two calcium dependent proteases from rabbit with different calcium sensitivities were isolated by high performance liquid chromatography (HPLC) and their properties were...
Purification and Properties of Pyruvate Kinase from Bacillus stearothermophilus (1986)
SAKAI, Hiroshi, SUZUKI, Koichi, IMAHORI, Kazutomo
Pyruvate kinase was purified to homogeneity from a moderate thermophile, Bacillus stearothermophilus. The molecular weight of the enzyme was found to be 250,000 on gel filtration and 242,000 on...
IMAJOH, Shinobu, KAWASAKI, Hiroshi, SUZUKI, Koichi
Ca2+-Activated neutral protease (CANP), that consists of 80K and 30K subunits, is converted to a 1ow-Ca2+-requiring form by autolysis in the presence of Ca Phosphatidylinositol greatly...
Gene organization of the small subunit of human calcium-activated neutral protease (1986)
Miyake, Sanae, Emori, Yasufumi, Suzuki, Koichi
The gene for the small subunit of human calcium-activated neutral protease was isolated and sequenced. It is 11 kb long and comprises 11 exons. No TATA or CAT box was found upstream of the possible...
IMAJOH, Shinobu, KAWASAKI, Hiroshi, SUZUKI, Koichi
Calcium-activated neutral protease (rabbit mCANP), composed of large and small subunits, was converted to a lower-Ca2+-requiring form (derived μCANP) by limited autolysis in the presence of...
TAGUCHI, Ryo, SUZUKI, Koichi, NAKABAYASHI, Toshikatsu, IKEZAWA, Hiroh
The mode of acetyicholinesterase release from mammalian erythrocyte membranes by the action of phosphatidylinositol(PI)-specific phospholipase C of Bacillus thuringiensis was studied. As regards...
SUZUKI, Koichi, ISHIURA, Shoichi
To clarify the mechanism of activation of calcium activated neutral protease (CANP, or mCANP: active at mM Ca2+), the structure of mCANP was examined by measuring CD spectra and by titration of SH...
IKEZAWA, Hiroh, NAKABAYASHI, Toshikatsu, SUZUKI, Koichi, NAKAJIMA, Masahiro, TAGUCHI, Toshiyuki, TAGUCHI, Ryo
A phosphatidylinositol-specific phospholiase C was purified from the culture broth of Bacillus thuringiensis IAM 12077 to a homogeneous state as revealed by polyacrylamide gel electrophoresis. The...
The reaction of calcium activated neutral protease (CANP) with an epoxysuccinyl derivative (E64c) and iodoacetic acid (IAA) was examined in the presence of Ca2+, which specifically accelerates the...
Connectin, an Elastic Protein of Muscle. Effects of Proteolytic Enzymes in Situ (1981)
MARUYAMA, Koscak, KIMURA, Masanori, KIMURA, Sumiko, OHASHI, Kazuyo, SUZUKI, Koichi, KATUNUMA, Nobuhiko
The effects of various proteolytic enzymes on the high molecular weight protein (connectin) present in a direct sodium dodecyl sulfate extract of myofibrils from chicken breast muscle were studied in...
SUZUKI, Yoshiyuki, SAKURABA, Hitoshi, HAYASHI, Kazuyo, SUZUKI, Koichi, IMAHORI, Kazutomo
β-Galactosidase was partially restored by protease inhibitors, leupeptin, chymo-statin and E-64 in cultured fibroblasts from three patients with β-galactosidase-neuraminidase deficiency. Pepstatin...
SUZUKI, Koichi, TSUJI, Shuichi, KUBOTA, Shunichiro, KIMURA, Yoshio, IMAHORI, Kazutomo
Ca2+-activated neutral protease (CANP) usually requires mM Ca2+ for activation. The sensitivity of CANP to Ca2+ is greatly enhanced by passing it through a casein-Sepharose column in the presence of...
Amino Acid Sequence of Immunity Protein (B Subunit) of ColicinE3 (1981)
MOCHITATE, Katsumi, SUZUKI, Koichi, IMAHORI, Kazutomo
The amino acid sequence of immunity protein (protein B) of colicin E3 (84 amino acid residues) was determined. B was first split into two fragments, CB 1 and CB 2, by CNBr treatment. The structure of...
TSUJI, Shuichi, ISHIURA, Shoichi, TAKAHASHT-NAKAMURA, Megumi, KATAMOTO, Tetsuo, SUZUKI, Koichi, IMAHORI, Kazutomo
The effects of Ca2+ ions on the structure and activity of Ca2+ neutral proteinase (CANP) were examined by means of carboxymethylation. CANP was inactivated by carboxymethylation of 1 mol of...
TAKAHASHI-NAKAMURA, Megumi, TSUJT, Shuichi, SUZUKI, Koichi, IMAHORI, Kazutomo
An endogenous inhibitor of calcium-activated neutral protease was purified to homogeneity from rabbit skeletal muscle using ion-exchange chromatography on DEAE-cellulose and QAE-Sephadex A-50...
Autolysis of Calcium-Activated Neutral Protease of Chicken Skeletal Muscle (1981)
SUZUKI, Koichi, TSUJI, Shuichi, ISHIURA, Shoichi, KIMURA, Yoshio, KUBOTA, Shunichiro, IMAHORI, Kazutomo
The conditions and process of autolysis of calcium-activated neutral protease (CANP) were examined. Optimal conditions for autolysis were the same as those required for expression of activity of...
SUZUKI, Koichi, OHNO, Shigeo, IMAHORI, Kazutomo
The physicochemical nature of an active fragment (T2A) of colicin E3 was examined together with those of its specific inhibitor, B, and the T2A-B complex under various conditions by means of CD and...
The Effects of Carboxypeptidase Digestion on the Function of Colicin E3 (1980)
OHNO, Shigeo, SAITO, Kimiko, SUZUKI, Koichi, IMAHORI, Kazutomo
The effects of carboxypeptidase digestion on the function of colicin E3 which had been demonstrated to be a complex of proteins A and B was investigated. It was indicated that removal of 7 amino...
WAKABAYASHI, Sadao, HASE, Toshiharu, WADA, Keishiro, MATSUBARA, Hiroshi, SUZUKI, Koichi
The amino acid sequences of two ferredoxins from Phytolacca esculenta were determined by a combination of solid-phase sequencing and the conventional methods. Ferredoxins I and II were composed of 96...
ISHIURA, Shoichi, SUGITA, Hideo, SUZUKI, Koichi, IMAHORI, Kazutomo
Calcium-activated neutral protease purified from chicken skeletal muscle hydrolyzed myo-fibrillar proteins, tubulin, spectrin, and oxidized insulin B chain, but hardly hydrolyzed synthetic or natural...
Studies on the Allosteric Nature of Acetate Kinase from Bacillus stearothermophilus (1979)
NAKAJIMA, Hiroshi, SUZUKI, Koichi, IMAHORI, Kazutomo
Fructose 1,6-bisphosphate (FBP) stimulates the reaction of Bacillus stearothermophilus acetate kinase (AK). FBP changes the reaction curve for ATP from a sigmoidal type to a Michaelis Menten one. The...
NAGATA, Kazuhiko, SUZUKI, Koichi, IMAHORI, Kazutomo
A reactive ATP analog, N6-(6-bromoacetamidohexyl)-AMP-PCP, reacted specifically with the ATP inhibitory site of rabbit skeletal muscle phosphofructokinase without affecting the active site....
Amino Acid Sequences of Two Ferredoxins from Pokeweed, Phytolacca americana (1978)
WAKABAYASHI, Sadao, HASE, Toshiharu, WADA, Keishiro, MATSUBARA, Hiroshi, SUZUKI, Koichi, TAKAICHI, Shinichi
The amino acid sequences of two ferredoxins isolated from pokeweed, Phytolacca americana, were determined. Tryptic peptides of maleyl-carboxymethyl-ferredoxin I and carboxy-methyl-ferredoxin II were...
Purification and Properties of Acetate Kinase from Bacillus stearothermophilus (1978)
NAKAJIMA, Hiroshi, SUZUKI, Koichi, IMAHORI, Kazutomo
1. Acetate kinase [EC 2.7.2.1] from an thermophile, B. stearothermophilus, was purified and crystallized. 2. This enzyme was shown to be a tetramer of identical subunits which had a molecular weight...
ISHIURA, Shoichi, MUROFUSHI, Hiromu, SUZUKI, Koichi, IMAHORI, Kazutomo
A calcium-activated neutral protease was purified 2,700-fold over the crude extract from chicken skeletal muscle. The purified protease migrated as a single band on polyacrylamide gel electrophoresis...
NAGATA, Kazuhiko, SUZUKI, Koichi, IMAHORI, Kazutomo
A reactive ATP analog, N4-(6-bromoacetamidohexyl)-AMP�PCP, was synthesized in an attempt to covalently label the binding sites for adenine nucleotides, especially ATP, of various enzymes which...
SUZUKI, Koichi, EGUCHI, Chikahiko, IMAHORI, Kazutomo
Affinity labeling of yeast and B. stearothermophilus phosphoglycerate kinases with a reactive AMP analog, N6-(p-bromoacetaminobenzyl)-AMP was examined. Complete loss of enzyme activity was observed...
EGUCHI, Chikahiko, SUZUKI, Koichi, IMAHORI, Kazutomo
In order to obtain a better understanding of the allosteric site of rabbit muscle phosphorylase b, nine AMP analogs having a bulky hydrophobic benzene ring were synthesized and tested for activity as...
SUZUKI, Koichi, EGUCHI, Chikahiko, IMAHORI, Kazuromo
Rabbit muscle glyceraldehyde 3-phosphate dehydrogenase (GPD) and myokinase (MK) were rapidly inactivated by a reactive AMP analog, N6-(p-bromoacetaminobenzyl)-AMP under mild conditions. Complete...
Purification and Characterization of Active Component and Active Fragment of Colicin E3 (1977)
OHNO, Shigeo, OHNO-IWASHITA, Yoshiko, SUZUKI, Koichi, IMAHORI, Kazutomo
Two components of colicin E3, namely proteins A and B, were prepared by means of an improved method. Protein A thus obtained was more than a thousand times as active as native colicin E3 when they...
Hybridization of Glyceraldehyde-3-phosphate Dehydrogenase in Borate (1976)
SUZUKI, Koichi, HIBINO, Ken, IMAHORI, Kazutomo
Conditions for the hybridization of glyceraldehyde-3-phosphate dehydrogenase (GPD) [EC 1. 2.1.12] in the presence of dilute borate were examined with horseshoe crab and rabbit GPDs. Hybridization was...
Hybridization of Glyceraldehyde-3-phosphate Dehydrogenase (1975)
SUZUKI, Koichi, HARRIS, J. Ieuan
Glyceraldehyde-3-phosphate dehydrogenases [EC 1. 2.1.12] from rabbit, pig, lobster, yeast, E. coli, and B. stearothermophilus have been subjected to hybridization in 3 M NaCl. Suitable mixtures of...
Glyceraldehyde-3-phosphate Dehydrogenase of Horseshoe Crab (Tachypleus tridentatus) (1975)
SUZUKI, Koichi, WATANABE, Motoó, IMAHORI, Kazutomo
Glyceraldehyde-3-phosphate dehydrogenase [EC 1.2.1.12] was purified from the horseshoe crab, a living fossil, and its properties were examined. The purified enzyme was homogeneous as judged by...
Fructose-diphophate Aldolase of Horseshoe Crab (Tachypleus tridentatus) (1975)
SUZUKI, Koichi, SHIHO, Osamu, IMAHORI, Kazutomo
Fructose-diphosphate aldolase [EC 4.1.2.13] was isolated from horseshoe crab (a living fossil) muscle and some molecular and enzymatic properties were examined. The enzyme was a tetramer with a...
DATE, Takayasu, SUZUKI, Koichi, IMAHORI, Kazutomo
DNA-dependent RNA polymerase [EC 2.7.7.6] was purified from the thermophile Thermus thermophilus HB8 and some properties were investigated. 1. Two kinds of the enzyme (enzymes A and B) were found and...
Phosphoglycerate Kinase of Bacillus stearothermophilus (1974)
SUZUKI, Koichi, IMAHORI, Kazutomo
Phosphoglycerate kinase [EC 2.7.2.3] was isolated in a homogeneous form from thermophilic bacteria, Bacillus stearothermophilus, and some properties were examined. The enzyme was composed of a...
SUZUKI, Koichi, IMAHORI, Kazutomo
1. Glyceraldehyde 3-phosphate dehydrogenase [GPD, EC 1. 2.1.12] from vegetative cells of Bacillus cereus was purified and its molecular and catalytic properties were examined. The purified enzyme was...
SUZUKI, Koichi, IMAHORI, Kazutomo
The structure of glyceraldehyde 3-phosphate dehydrogenase [EC 1. 2.1.12] of B. stearothermophilus was investigated by circular dichroism and fluorescence emission spectro-scopy under various...
Studies on Protamines: XVI. The Complete Amino Acid Sequence of Clupeine YII (1972)
Clupeine from Pacific herring was fractionated into Z and Y fractions by chromato-graphy on CM-cellulose. After trinitrophenylation of the clupeine Y fraction with 2, 4, 6-trinitrobenzenesulfonic...
Studies on Protamines: XVII. The Complete Amino Acid Sequence of Clupeine YI (1972)
Clupeine extracted from sperm nuclei of Pacific herring is composed of three main molecular species, YI, YII, and Z. The complete amino acid sequences of the two components Z and YII have already...
Studies on Protamines: XIII. The Fractionation of Clupeine Y (1968)
1. More reproducible and satisfactory chromatographic systems on CM-cellulose (see Fig. 2) were found for fractionation of whole clupeine into Y and Z fractions in place of the previous method by...
Strobl, Stefan, Fernandez-Catalan, Carlos, Braun, Marianne, Huber, Robert, Masumoto, Hajime, Nakagawa, Kazuhiro, ...
Calpains (calcium-dependent cytoplasmic cysteine proteinases) are implicated in processes such as cytoskeleton remodeling and signal transduction. The 2.3-Å crystal structure of full-length...
Autoregulation of thyroid-specific gene transcription by thyroglobulin
Suzuki, Koichi, Lavaroni, Stefano, Mori, Atsumi, Ohta, Masanori, Saito, Jun, Pietrarelli, Michele, ...
Thyroglobulin (TG), the primary synthetic product of the thyroid, is the macromolecular precursor of thyroid hormones. TG synthesis, iodination, storage in follicles, and degradation control thyroid...
Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides
Suzuki, Koichi, Mori, Atsumi, Ishii, Ken J., Saito, Jun, Singer, Dinah S., Klinman, Dennis M., ...
Abnormal expression of major histocompatibility complex (MHC) class I and class II in various tissues is associated with autoimmune disease. Autoimmune responses can be triggered by viral infections...
Royaux, Ines E., Wall, Susan M., Karniski, Lawrence P., Everett, Lorraine A., Suzuki, Koichi, Knepper, Mark A., ...
Pendrin is an anion transporter encoded by the PDS/Pds gene. In humans, mutations in PDS cause the genetic disorder Pendred syndrome, which is associated with deafness and goiter. Previous studies...
Nakazato, Minoru, Chung, Hyun-Kyung, Ulianich, Luca, Grassadonia, Antonino, Suzuki, Koichi, Kohn, Leonard D.
Follicular thyroglobulin (TG) selectively suppresses the expression of thyroid-restricted transcription factors, thereby altering the expression of thyroid-specific proteins. In this study, we...
Herasse, Muriel, Ono, Yasuko, Fougerousse, Françoise, Kimura, Ei-ichi, Stockholm, Daniel, Beley, Cyriaque, ...
Calpain 3 is a nonlysosomal cysteine protease whose biological functions remain unknown. We previously demonstrated that this protease is altered in limb girdle muscular dystrophy type 2A patients....
Intracellular Redistribution of Truncated La Protein Produced by Poliovirus 3Cpro-Mediated Cleavage
Shiroki, Kazuko, Isoyama, Takeshi, Kuge, Shusuke, Ishii, Toshihiko, Ohmi, Shinobu, Hata, Syoji, ...
The La autoantigen (also known as SS-B), a cellular RNA binding protein, may shuttle between the nucleus and cytoplasm, but it is mainly located in the nucleus. La protein is redistributed to the...
Suzuki, Koichi, Lavaroni, Stefano, Mori, Atsumi, Okajima, Fumikazu, Kimura, Shioko, Katoh, Ryohei, ...
Thyroid transcription factor 1 (TTF-1) was identified for its critical role in thyroid-specific gene expression; its level in the thyroid is regulated by thyrotropin-increased cyclic AMP levels....
Hashimoto, Ken, Maeda, Yumi, Kimura, Hiroaki, Suzuki, Koichi, Masuda, Akihiro, Matsuoka, Masanori, ...
Host defense against Mycobacterium leprae infection is chiefly mediated by gamma interferon (IFN-γ)-secreting cytotoxic T cells. Since which antigen-presenting cell populations act to stimulate...
Kawahara, Hiroyuki, Kasahara, Masanori, Nishiyama, Atsuya, Ohsumi, Keita, Goto, Tetsuya, Kishimoto, Takeo, ...
The 26S proteasome is a multisubunit protein- destroying machinery that degrades ubiquitin-tagged proteins. To date only a single species of Rpn10, which possibly functions as a multiubiquitin...
NISHIO, HAJIME, MATSUI, KIYOSHI, TSUJI, HIROKO, TAMURA, AKIYOSHI, SUZUKI, KOICHI
The janus kinases (JAK) and signal transducers and activators of the transcription (STAT) pathway have been shown to be activated by a number of cytokines or growth factors and to play significant...
Takeshita, Fumihiko, Tanaka, Toshiyuki, Matsuda, Tomoko, Tozuka, Miyuki, Kobiyama, Kouji, Saha, Sukumar, ...
Toll-like receptors (TLRs) recognize microbial components and trigger the signaling cascade that activates the innate and adaptive immunity. TLR adaptor molecules play a central role in this cascade;...
Strobl, Stefan, Fernandez-Catalan, Carlos, Braun, Marianne, Huber, Robert, Masumoto, Hajime, Nakagawa, Kazuhiro, ...
Calpains (calcium-dependent cytoplasmic cysteine proteinases) are implicated in processes such as cytoskeleton remodeling and signal transduction. The 2.3-Å crystal structure of full-length...
Autoregulation of thyroid-specific gene transcription by thyroglobulin
Suzuki, Koichi, Lavaroni, Stefano, Mori, Atsumi, Ohta, Masanori, Saito, Jun, Pietrarelli, Michele, ...
Thyroglobulin (TG), the primary synthetic product of the thyroid, is the macromolecular precursor of thyroid hormones. TG synthesis, iodination, storage in follicles, and degradation control thyroid...
Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides
Suzuki, Koichi, Mori, Atsumi, Ishii, Ken J., Saito, Jun, Singer, Dinah S., Klinman, Dennis M., ...
Abnormal expression of major histocompatibility complex (MHC) class I and class II in various tissues is associated with autoimmune disease. Autoimmune responses can be triggered by viral infections...
Royaux, Ines E., Wall, Susan M., Karniski, Lawrence P., Everett, Lorraine A., Suzuki, Koichi, Knepper, Mark A., ...
Pendrin is an anion transporter encoded by the PDS/Pds gene. In humans, mutations in PDS cause the genetic disorder Pendred syndrome, which is associated with deafness and goiter. Previous studies...
Nakazato, Minoru, Chung, Hyun-Kyung, Ulianich, Luca, Grassadonia, Antonino, Suzuki, Koichi, Kohn, Leonard D.
Follicular thyroglobulin (TG) selectively suppresses the expression of thyroid-restricted transcription factors, thereby altering the expression of thyroid-specific proteins. In this study, we...
Herasse, Muriel, Ono, Yasuko, Fougerousse, Françoise, Kimura, Ei-ichi, Stockholm, Daniel, Beley, Cyriaque, ...
Calpain 3 is a nonlysosomal cysteine protease whose biological functions remain unknown. We previously demonstrated that this protease is altered in limb girdle muscular dystrophy type 2A patients....
Intracellular Redistribution of Truncated La Protein Produced by Poliovirus 3Cpro-Mediated Cleavage
Shiroki, Kazuko, Isoyama, Takeshi, Kuge, Shusuke, Ishii, Toshihiko, Ohmi, Shinobu, Hata, Syoji, ...
The La autoantigen (also known as SS-B), a cellular RNA binding protein, may shuttle between the nucleus and cytoplasm, but it is mainly located in the nucleus. La protein is redistributed to the...
Suzuki, Koichi, Lavaroni, Stefano, Mori, Atsumi, Okajima, Fumikazu, Kimura, Shioko, Katoh, Ryohei, ...
Thyroid transcription factor 1 (TTF-1) was identified for its critical role in thyroid-specific gene expression; its level in the thyroid is regulated by thyrotropin-increased cyclic AMP levels....
Hashimoto, Ken, Maeda, Yumi, Kimura, Hiroaki, Suzuki, Koichi, Masuda, Akihiro, Matsuoka, Masanori, ...
Host defense against Mycobacterium leprae infection is chiefly mediated by gamma interferon (IFN-γ)-secreting cytotoxic T cells. Since which antigen-presenting cell populations act to stimulate...
Kawahara, Hiroyuki, Kasahara, Masanori, Nishiyama, Atsuya, Ohsumi, Keita, Goto, Tetsuya, Kishimoto, Takeo, ...
The 26S proteasome is a multisubunit protein- destroying machinery that degrades ubiquitin-tagged proteins. To date only a single species of Rpn10, which possibly functions as a multiubiquitin...
NISHIO, HAJIME, MATSUI, KIYOSHI, TSUJI, HIROKO, TAMURA, AKIYOSHI, SUZUKI, KOICHI
The janus kinases (JAK) and signal transducers and activators of the transcription (STAT) pathway have been shown to be activated by a number of cytokines or growth factors and to play significant...
Takeshita, Fumihiko, Tanaka, Toshiyuki, Matsuda, Tomoko, Tozuka, Miyuki, Kobiyama, Kouji, Saha, Sukumar, ...
Toll-like receptors (TLRs) recognize microbial components and trigger the signaling cascade that activates the innate and adaptive immunity. TLR adaptor molecules play a central role in this cascade;...
Localization of CORO1A in the Macrophages Containing Mycobacterium leprae
Suzuki, Koichi, Takeshita, Fumihiko, Nakata, Noboru, Ishii, Norihisa, Makino, Masahiko
Mycobacteria have acquired an intracellular lifestyle within the macrophage, which is best exemplified by the enlarged infected histiocytes seen in lepromatous leprosy. To survive within the cell,...
Konishi, Yukiko, Suzuki, Koichi, Wada, Hidetoshi, Watanabe, Hiroshi, Ogura, Hiroyuki, Sugamori, Yuno, ...
Rocchi, Roberto, Kimura, Hiroaki, Tzou, Shey-Cherng, Suzuki, Koichi, Rose, Noel R., Pinchera, Aldo, ...
Bacterial infections and other pathologic conditions induce complex dysfunctions of the hypothalamic–pituitary–thyroid axis, collectively known as nonthyroidal illness (NTI). To explore the...
Suzuki, Koichi, Han, Gi Dong, Miyauchi, Naoko, Hashimoto, Taeko, Nakatsue, Takeshi, Fujioka, Yumiko, ...
Although angiotensin II (Ang II) type 1 receptor antagonist ameliorates proteinuria, its pharmacological mechanism and the differential roles of Ang II type 1 receptor (AT1R) and type 2 receptor...
The Atg5–Atg12 conjugate associates with innate antiviral immune responses
Jounai, Nao, Takeshita, Fumihiko, Kobiyama, Kouji, Sawano, Asako, Miyawaki, Atsushi, Xin, Ke-Qin, ...
Autophagy is an essential process for physiological homeostasis, but its role in viral infection is only beginning to be elucidated. We show here that the Atg5–Atg12 conjugate, a key regulator of...
Nakanaga, Kazue, Ishii, Norihisa, Suzuki, Koichi, Tanigawa, Kazunari, Goto, Masamichi, Okabe, Tsutomu, ...
We describe the fourth reported case involving “Mycobacterium ulcerans subsp. shinshuense.” Compared to previous cases, the infection was more invasive with extensive ulceration from the elbow to...
Gregorio, Carol C., Trombitás, Karoly, Centner, Thomas, Kolmerer, Bernhard, Stier, Gunter, Kunke, Kathleen, ...
Titin is a giant elastic protein in vertebrate striated muscles with an unprecedented molecular mass of 3–4 megadaltons. Single molecules of titin extend from the Z-line to the M-line. Here, we...
Akama, Takeshi, Suzuki, Koichi, Tanigawa, Kazunari, Kawashima, Akira, Wu, Huhehasi, Nakata, Noboru, ...
Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously...
Fukunishi, Shinya, Nishio, Hajime, Fukuda, Akira, Takeshita, Atsushi, Hanafusa, Toshiaki, Higuchi, Kazuhide, ...
Nonalcoholic steatohepatitis (NASH) can develop into end-stage disease such as cryptogenic cirrhosis and hepatocellular carcinoma. Hence, it is important to understand the pathogenesis of NASH. In...