Menon, Suchithra, Tsuge, Tomohiko, Dohmae, Naoshi, Takio, Koji, Wei, Ning
Abstract Background Cullin-RING ubiquitin E3 ligases (CRLs) are regulated by modification of an ubiquitin-like protein, Nedd8 (also known as Rub1) on the cullin subunit. Neddylation is shown to...
Yokoyama, Kazuaki, Nakagawa, Miyuki, Satoh, Masayuki, Saitoh, Shigeaki, Dohmae, Naoshi, Harada, Ayako, ...
The fission yeast lsd1/fas2 strain carries a temperature-sensitive mutation of the fatty-acid-synthase α-subunit, exhibiting an aberrant mitosis lsd phenotype, with accumulation of very-long-chain...
Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshiiOT3 (2005)
Usui, Kengo, Katayama, Shintaro, Kanamori-Katayama, Mutsumi, Ogawa, Chihiro, Kai, Chikatoshi, Okada, Makiko, ...
Abstract Background Although 2,061 proteins of Pyrococcus horikoshii OT3, a hyperthermophilic archaeon, have been predicted from the recently completed genome sequence, the majority of proteins show...
Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...
Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....
Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...
Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....
Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...
Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....
Korekane, Hiroaki, Taguchi, Tomohiko, Sakamoto, Yoshihiro, Honke, Koichi, Dohmae, Naoshi, Salminen, Heidi, ...
A rat intestinal β1,6N-acetylglucosaminyltransferase (β1-6GnT) responsible for the formation of the β1,6-branched poly-N-acetyllactosamine structure has been purified to apparent homogeneity by...
Korekane, Hiroaki, Taguchi, Tomohiko, Sakamoto, Yoshihiro, Honke, Koichi, Dohmae, Naoshi, Salminen, Heidi, ...
A rat intestinal β1,6N-acetylglucosaminyltransferase (β1-6GnT) responsible for the formation of the β1,6-branched poly-N-acetyllactosamine structure has been purified to apparent homogeneity by...
Human mitochondrial DNA is packaged with TFAM (2003)
Alam, Tanfis Istiaq, Kanki, Tomotake, Muta, Tsuyoshi, Ukaji, Koutarou, Abe, Yoshito, Nakayama, Hiroshi, ...
Mitochondrial transcription factor A (TFAM), a member of the high mobility group proteins, is essential for maintenance of mitochondrial DNA (mtDNA). Most TFAM and mtDNA (both of which are normally...
Korekane, Hiroaki, Taguchi, Tomohiko, Sakamoto, Yoshihiro, Honke, Koichi, Dohmae, Naoshi, Salminen, Heidi, ...
A rat intestinal β1,6N-acetylglucosaminyltransferase (β1-6GnT) responsible for the formation of the β1,6-branched poly-N-acetyllactosamine structure has been purified to apparent homogeneity by...
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells (2002)
Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...
A suppressor tRNATyr and mutant tyrosyl‐tRNA synthetase (TyrRS) pair was developed to incorporate 3‐iodo‐l‐tyrosine into proteins in mammalian cells. First, the Escherichia coli...
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes (2000)
Matsumoto, Ken, Aoki, Kazuma, Dohmae, Naoshi, Takio, Koji, Tsujimoto, Masafumi
In an attempt to isolate mRNA-binding proteins we fractionated Xenopus oocyte lysate by oligo(dT)–cellulose chromatography. A 20 kDa protein was the major component of the eluate. cDNA cloning...
Wakatsuki, Shuji, Arioka, Manabu, Dohmae, Naoshi, Takio, Koji, Yamasaki, Makari, Kitamoto, Katsuhiko
In our previous paper, we reported that a 15 kDa protein (pl5) produced by a fungus, genus Helicosporium, enhanced NGF-induced neurite outgrowth from PC12 cells. Here we further characterized the...
The relationship between ST6Gal I Golgi retention and its cleavage-secretion (1999)
Kitazume-Kawaguchi, Shinobu, Dohmae, Naoshi, Takio, Koji, Tsuji, Shuichi, Colley, Karen J.
The ST6Gal I is a sialyltransferase that modifies N-linked oligosaccharides of glycoproteins. Previous results suggested a role for luminal stem and active domain sequences in the efficiency of...
Nojiri, Masaki, Yohda, Masafumi, Odaka, Masafumi, Matsushita, Yusuke, Tsujimura, Masanari, Yoshida, Takao, ...
The nitrile hydratase (NHase) from Rhodococcus sp. N-771 is a photoreactive enzyme that is inactivated on nitrosylation of the non-heme iron center and activated on photo-dissociation of nitric oxide...
Sutani, Takashi, Yuasa, Tatsuro, Tomonaga, Takeshi, Dohmae, Naoshi, Takio, Koji, Yanagida, Mitsuhiro
Akaba, Shuichi, Seo, Mitsunori, Dohmae, Naoshi, Takio, Koji, Sekimoto, Hiroyuki, Kamiya, Yuji, ...
Polyclonal antibodies were raised against synthetic peptides or recombinant polypeptides encoded by Arabidopsis atAO-1 and atAO-2 cDNAs, which have sequences similar to maize and animal aldehyde...
Harada, Ayako, Takeuchi, Ken-ichi, Dohmae, Naoshi, Takio, Koji, Uenaka, Toshimitu, Aoki, Junken, ...
Immunoblotting showed that a monoclonal antibody, 3A10, binds to a series of rat brain-specific antigens with molecular masses of 150-,120-,118-,106-,104-,79-, and 77-kDa. The expression of 3A10...
Sekimoto, Hiroyuki, Fukumoto, Ryo-hei, Dohmae, Naoshi, Takio, Koji, Fujii, Tadashi, Kamiya, Yuji
A sex pheromone, protoplast-release-inducing protein (PR-IP) inducer, of the Closterium peracerosum-stri-gosum-littorale complex is known to induce the release of PR-IP, from mating-type plus (mt+)...
Yeo, Dennis, Abe, Tomoko, Abe, Hiroshi, Sakurai, Akira, Takio, Koji, Dohmae, Naoshi, ...
Asparagus officinalis seedlings treated with either thiocarbamates or carbamates are induced to flower within 2-3 weeks of application. SDS-PAGE and 2-D PAGE resolutions of total soluble proteins of...
Site-Specific Glycosylation of Bovine Butyrophilin (1995)
Sato, Takeshi, Takio, Koji, Kobata, Akira, Greenwalt, Dale E., Furukawa, Kiyoshi
Our previous studies showed that the N-linked sugar chains of most bovine glycoproteins from milk fat globule membranes (MFGM) contain the GalNAcβ1→4GlcNAc group [Sato et al. (1993) J. Biochem....
Nonaka, Takashi, Ishikawa, Hiroko, Tsumuraya, Yoichi, Hashimoto, Yoichi, Dohmae, Naoshi, Takio, Koji
A zinc-metalloendopeptidase, MEP, capable of catalyzing specific cleavage of acyl-lysine bonds (-X-Lys-) in polypeptides has been purified 212-fold in a yield of 24.7% from the fruiting bodies of...
Lee, In Sook Matsui, Takio, Koji, Kido, Ryo, Titani, Koiti
In order to confirm the amino acid sequence predicted from the nucleotide sequence of cDNA and also to elucidate the intracellular localization and molecular evolution, human liver alanine-glyoxylate...
Tomizawa, Ken-ichi, Ito, Naoko, Komeda, Yoshibumi, Uyeda, Taro Q. P., Takio, Koji, Furuya, Masaki
The pea phytochrome I (PI) cDNA clone, pPP1001, was expressed in E. coli. The plasmid pPP1001 contains pea PI cDNA which covers the entire coding region with the Shine-Dalgarno consensus sequence...
Abe, Hiroshi, Takio, Koji, Titani, Koiti, Furuya, Masaki
Apical shoot of pea seedling contains two immunochemically distinct species of phytochrome, type I (PI) and type II (PII) [Abe et al. (1985) Plant Cell Physiol. 26: 1387-1399]. A PII sample was...
Ikemoto, Mamoru, Arai, Hiroyuki, Feng, Dongdong, Tanaka, Kazumi, Aoki, Junken, Dohmae, Naoshi, ...
The scavenger receptor class B type I (SR-BI) mediates the selective uptake of cholesteryl esters from high-density lipoprotein (HDL) and cholesterol secretion into bile in the liver. In this study,...
Terayama, Koji, Oka, Shogo, Seiki, Takashi, Miki, Yukari, Nakamura, Akemi, Kozutsumi, Yasunori, ...
The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules and also on some glycolipids in the nervous system over a wide range of species from insect to...
Shibata, Norihito, Arita, Makoto, Misaki, Yuko, Dohmae, Naoshi, Takio, Koji, Ono, Teruo, ...
Squalene epoxidase, a membrane-associated enzyme that converts squalene to squalene 2,3-oxide, plays an important role in the maintenance of cholesterol homeostasis. In 1957, Bloch and colleagues...
Raj, V. Samuel, Tomitori, Hideyuki, Yoshida, Madoka, Apirakaramwong, Auayporn, Kashiwagi, Keiko, Takio, Koji, ...
Escherichia coli CAG2242 cells are deficient in the speG gene encoding spermidine acetyltransferase. When these cells were cultured in the presence of 0.5 to 4 mM spermidine, their viability was...
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes
Matsumoto, Ken, Aoki, Kazuma, Dohmae, Naoshi, Takio, Koji, Tsujimoto, Masafumi
In an attempt to isolate mRNA-binding proteins we fractionated Xenopus oocyte lysate by oligo(dT)–cellulose chromatography. A 20 kDa protein was the major component of the eluate. cDNA cloning...
Kiga, Daisuke, Sakamoto, Kensaku, Kodama, Koichiro, Kigawa, Takanori, Matsuda, Takayoshi, Yabuki, Takashi, ...
Tyrosyl–tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-l-tyrosine rather than l-tyrosine for the site-specific incorporation of 3-iodo-l-tyrosine...
Hashimoto, Tadafumi, Wakabayashi, Tomoko, Watanabe, Atsushi, Kowa, Hisatomo, Hosoda, Ritsuko, Nakamura, Atsushi, ...
We raised monoclonal antibodies against senile plaque (SP) amyloid and obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with ∼50/100 kDa polypeptides in Alzheimer’s disease...
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...
A suppressor tRNATyr and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNATyr...
Human mitochondrial DNA is packaged with TFAM
Istiaq Alam, Tanfis, Kanki, Tomotake, Muta, Tsuyoshi, Ukaji, Koutarou, Abe, Yoshito, Nakayama, Hiroshi, ...
Mitochondrial transcription factor A (TFAM), a member of the high mobility group proteins, is essential for maintenance of mitochondrial DNA (mtDNA). Most TFAM and mtDNA (both of which are normally...
Sutani, Takashi, Yuasa, Tatsuro, Tomonaga, Takeshi, Dohmae, Naoshi, Takio, Koji, Yanagida, Mitsuhiro
The condensin complex in frog extracts, containing two SMC (structural maintenance of chromosomes) and three non-SMC subunits, promotes mitotic chromosome condensation, and its supercoiling activity...
Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi
Hirose, Hidenori, Arasaki, Kohei, Dohmae, Naoshi, Takio, Koji, Hatsuzawa, Kiyotaka, Nagahama, Masami, ...
ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in...
Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...
Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....
Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3
Usui, Kengo, Katayama, Shintaro, Kanamori-Katayama, Mutsumi, Ogawa, Chihiro, Kai, Chikatoshi, Okada, Makiko, ...
Protein-protein interactions among 960 Pyrococcus soluble proteins have been analysed by mammalian two-hybrid analysis and thirteen interactions between annotated and unannotated proteins detected.
Ikemoto, Mamoru, Arai, Hiroyuki, Feng, Dongdong, Tanaka, Kazumi, Aoki, Junken, Dohmae, Naoshi, ...
The scavenger receptor class B type I (SR-BI) mediates the selective uptake of cholesteryl esters from high-density lipoprotein (HDL) and cholesterol secretion into bile in the liver. In this study,...
Terayama, Koji, Oka, Shogo, Seiki, Takashi, Miki, Yukari, Nakamura, Akemi, Kozutsumi, Yasunori, ...
The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules and also on some glycolipids in the nervous system over a wide range of species from insect to...
Shibata, Norihito, Arita, Makoto, Misaki, Yuko, Dohmae, Naoshi, Takio, Koji, Ono, Teruo, ...
Squalene epoxidase, a membrane-associated enzyme that converts squalene to squalene 2,3-oxide, plays an important role in the maintenance of cholesterol homeostasis. In 1957, Bloch and colleagues...
Raj, V. Samuel, Tomitori, Hideyuki, Yoshida, Madoka, Apirakaramwong, Auayporn, Kashiwagi, Keiko, Takio, Koji, ...
Escherichia coli CAG2242 cells are deficient in the speG gene encoding spermidine acetyltransferase. When these cells were cultured in the presence of 0.5 to 4 mM spermidine, their viability was...
CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes
Matsumoto, Ken, Aoki, Kazuma, Dohmae, Naoshi, Takio, Koji, Tsujimoto, Masafumi
In an attempt to isolate mRNA-binding proteins we fractionated Xenopus oocyte lysate by oligo(dT)–cellulose chromatography. A 20 kDa protein was the major component of the eluate. cDNA cloning...
Kiga, Daisuke, Sakamoto, Kensaku, Kodama, Koichiro, Kigawa, Takanori, Matsuda, Takayoshi, Yabuki, Takashi, ...
Tyrosyl–tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-l-tyrosine rather than l-tyrosine for the site-specific incorporation of 3-iodo-l-tyrosine...
Hashimoto, Tadafumi, Wakabayashi, Tomoko, Watanabe, Atsushi, Kowa, Hisatomo, Hosoda, Ritsuko, Nakamura, Atsushi, ...
We raised monoclonal antibodies against senile plaque (SP) amyloid and obtained a clone 9D2, which labeled amyloid fibrils in SPs and reacted with ∼50/100 kDa polypeptides in Alzheimer’s disease...
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
Sakamoto, Kensaku, Hayashi, Akiko, Sakamoto, Ayako, Kiga, Daisuke, Nakayama, Hiroshi, Soma, Akiko, ...
A suppressor tRNATyr and mutant tyrosyl-tRNA synthetase (TyrRS) pair was developed to incorporate 3-iodo-l-tyrosine into proteins in mammalian cells. First, the Escherichia coli suppressor tRNATyr...
Human mitochondrial DNA is packaged with TFAM
Istiaq Alam, Tanfis, Kanki, Tomotake, Muta, Tsuyoshi, Ukaji, Koutarou, Abe, Yoshito, Nakayama, Hiroshi, ...
Mitochondrial transcription factor A (TFAM), a member of the high mobility group proteins, is essential for maintenance of mitochondrial DNA (mtDNA). Most TFAM and mtDNA (both of which are normally...
Sutani, Takashi, Yuasa, Tatsuro, Tomonaga, Takeshi, Dohmae, Naoshi, Takio, Koji, Yanagida, Mitsuhiro
The condensin complex in frog extracts, containing two SMC (structural maintenance of chromosomes) and three non-SMC subunits, promotes mitotic chromosome condensation, and its supercoiling activity...
Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi
Hirose, Hidenori, Arasaki, Kohei, Dohmae, Naoshi, Takio, Koji, Hatsuzawa, Kiyotaka, Nagahama, Masami, ...
ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in...
Aihara, Hitoshi, Nakagawa, Takeya, Yasui, Kiyoshi, Ohta, Tsutomu, Hirose, Susumu, Dhomae, Naoshi, ...
Posttranslational histone modifications are important for the regulation of many biological phenomena. Here, we show the purification and characterization of nucleosomal histone kinase-1 (NHK-1)....
Protein-protein interactions of the hyperthermophilic archaeon Pyrococcus horikoshii OT3
Usui, Kengo, Katayama, Shintaro, Kanamori-Katayama, Mutsumi, Ogawa, Chihiro, Kai, Chikatoshi, Okada, Makiko, ...
Protein-protein interactions among 960 Pyrococcus soluble proteins have been analysed by mammalian two-hybrid analysis and thirteen interactions between annotated and unannotated proteins detected.
Plant cells recognize chitin fragments for defense signaling through a plasma membrane receptor
Kaku, Hanae, Nishizawa, Yoko, Ishii-Minami, Naoko, Akimoto-Tomiyama, Chiharu, Dohmae, Naoshi, Takio, Koji, ...
Chitin is a major component of fungal cell walls and serves as a molecular pattern for the recognition of potential pathogens in the innate immune systems of both plants and animals. In plants,...
Dreanno, Catherine, Matsumura, Kiyotaka, Dohmae, Naoshi, Takio, Koji, Hirota, Hiroshi, Kirby, Richard R., ...
Many benthic marine invertebrates, like barnacles, have a planktonic larval stage whose primary purpose is dispersal. How these species colonize suitable substrata is fundamental to understanding...
Umezu-Goto, Makiko, Kishi, Yasuhiro, Taira, Akitsu, Hama, Kotaro, Dohmae, Naoshi, Takio, Koji, ...
Autotaxin (ATX) is a tumor cell motility–stimulating factor, originally isolated from melanoma cell supernatants. ATX had been proposed to mediate its effects through 5′-nucleotide...
Ohshima, Noriyasu, Yamashita, Saori, Takahashi, Naoko, Kuroishi, Chizu, Shiro, Yoshitsugu, Takio, Koji
Escherichia coli cytosolic glycerophosphodiester phosphodiesterase, UgpQ, functions in the absence of other proteins encoded by the ugp operon and requires Mg2+, Mn2+, or Co2+, in contrast to...
Structure of the stand-alone RAM-domain protein from Thermus thermophilus HB8
Nakano, Noboru, Okazaki, Nobuo, Satoh, Shinya, Takio, Koji, Kuramitsu, Seiki, Shinkai, Akeo, ...
The crystal structure of the stand-alone RAM domain from T. thermophilus HB8 has been determined at 2.4 Å resolution. The structure revealed that five dimers are arranged to form a ring.
Crystal Structure of Squid Rhodopsin with Intracellularly Extended Cytoplasmic Region*S⃞
Shimamura, Tatsuro, Hiraki, Kenji, Takahashi, Naoko, Hori, Tetsuya, Ago, Hideo, Masuda, Katsuyoshi, ...
G-protein-coupled receptors play a key step in cellular signal transduction cascades by transducing various extracellular signals via G-proteins. Rhodopsin is a prototypical G-protein-coupled...