Koji Tomoo

Turnip Mosaic Virus Genome-Linked Protein VPg Binds C-Terminal Region of Cap-Bound Initiation Factor 4E Orthologue Without Exhibiting Host Cellular Specificity (2009)

Okade, Hayato, Fujita, Yuki, Miyamoto, Saori, Tomoo, Koji, Muto, Shinji, Miyoshi, Hiroshi, ...

To investigate the binding specificity of turnip mosaic virus (TuMV) viral protein-genome linked (VPg) with translation initiation factor 4E, we evaluated here the kinetic parameters for the...

Domain-dependent Interaction of Eukaryotic Initiation Factor eIF4A for Binding to Middle and C-terminal Domains of eIF4G (2009)

Fujita, Yuki, Oe, Masako, Tutsumino, Tatsuya, Morino, Shigenobu, Imataka, Hiroaki, Tomoo, Koji, ...

The interactions of recombinant human eIF4A (4A) and its N- and C-terminal side domains (AN and AC, respectively) with the middle- and C-terminal-domain-linked fragment (GMC) of eIF4G and its middle...

Marked Difference Between Self-aggregations of First and Fourth Repeat Peptides on Tau Microtubule-binding Domain in Acidic Solution (2007)

Mizushima, Fumie, Minoura, Katsuhiko, Tomoo, Koji, Sumida, Miho, Taniguchi, Taizo, Ishida, Toshimasa

The heparin-induced self-aggregation behaviours of four repeat peptides (R1–R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and...

Effect of N-Terminal Region of eIF4E and Ser65-Phosphorylation of 4E-BP1 on Interaction between eIF4E and 4E-BP1 Fragment Peptide (2006)

Tomoo, Koji, Abiko, Fumi, Miyagawa, Hiroo, Kitamura, Kunihiro, Ishida, Toshimasa

To clarify the contribution of N-terminal region of eukaryotic initiation factor 4E (eIF4E) to the interaction with 4E-BP and to investigate the effect of 4E-BP phosphorylation on the interaction...

Effect of N-Terminal Region of eIF4E and Ser65-Phosphorylation of 4E-BP1 on Interaction between eIF4E and 4E-BP1 Fragment Peptide (2006)

Tomoo, Koji, Abiko, Fumi, Miyagawa, Hiroo, Kitamura, Kunihiro, Ishida, Toshimasa

To clarify the contribution of N-terminal region of eukaryotic initiation factor 4E (eIF4E) to the interaction with 4E-BP and to investigate the effect of 4E-BP phosphorylation on the interaction...

Function of AGCA tetrads in (CAG)n repeats (2004)

Grzeskowiak, Kazimierz, Sawaya, Michael R., Ohishi, Hirofumi, Tomoo, Koji, Ishida, Toshimasa

We investigate the structural properties of d(CAG)n repeats in d[CG(CAG)4CG)]*d[CG(CTG)4CG]. The crystals diffract up to 3.5 Å and completeness of data is only 60%. We also measure Tm-UV and CD...

Aggregation Analysis of the Microtubule Binding Domain in Tau Protein by Spectroscopic Methods (2003)

Yao, Tian-Ming, Tomoo, Koji, Ishida, Toshimasa, Hasegawa, Hiroshi, Sasaki, Masahiro, Taniguchi, Taizo

The microtubule-associated protein tau is a highly soluble protein that shows hardly any tendency to assemble under physiological conditions. In the brains of Alzheimer’s disease (AD) patients,...

Binding Diversity of a Noncovalent-Type Low-Molecular-Weight Serine Protease Inhibitor and Function of a Catalytic Water Molecule: X-Ray Crystal Structure of PKSI-527-Inhibited Trypsin (2001)

Tomoo, Koji, Satoh, Kazuhiro, Tsuda, Yuko, Wanaka, Keiko, Okamoto, Shosuke, Hijikata-Okunomiya, Akiko, ...

PKSI-527 is a noncovalent-type low-molecular-weight inhibitor. The X-ray crystal structure of the trypsin-PKSI-527 complex revealed a binding mode (Form II) different from the previously reported one...

Substrate Specificity of Bovine Cathepsin B and Its Inhibition by CA074, Based on Crystal Structure Refinement of the Complex (2000)

Yamamoto, Atsushi, Tomoo, Koji, Hara, Tadaoki, Murata, Mitsuo, Kitamura, Kunihiro, Ishida, Toshimasa

The crystal structure of the bovine spleen cathepsin B (BSCB)-CA074 complex was refined to R = 0.152 using X-ray diffraction data up to 2.18 A resolution. BSCB is characterized by an extra...

Function of the Propeptide Region in Recombinant Expression of Active Procathepsin L in Escherichia coli (1999)

Ogino, Tetsuya, Kaji, Toshio, Kawabata, Mitsuhiko, Satoh, Kazumasa, Tomoo, Koji, Ishida, Toshimasa, ...

In order to determine the functional role of the procathepsin L propeptide region for the preparation of active recombinant rat cathepsin L (CL), cDNAs encoding two short-length propeptides...

Expression of a Synthetic Gene for Initiation Factor 4E-Binding Protein 1 in Escherichia coli and Its Interaction with eIF-4E and eIF-4E-m7GTP Complex (1998)

Nishi, Noriko, Morino, Shigenobu, Tomoo, Koji, Youtani, Takeshi, Ishida, Toshimasa

An artificial gene coding for the human initiation factor (elF) 4E-binding protein 1 (4E-BP1) was chemically synthesized and cloned. Although the expression of the 4E-BP1 gene alone has not yet been...

Binding Mode of CA074, a Specific Irreversible Inhibitor, to Bovine Cathepsin B as Determined by X-Ray Crystal Analysis of the Complex (1997)

Yamamoto, Atsushi, Hara, Tadaoki, Tomoo, Koji, Ishida, Toshimasa, Fujii, Tomomi, Hata, Yasuo, ...

The binding mode of CA074 [N-(L-3-trans-propylcarbamoyl-oxirane-2-carbonyl)-L-isoleucyl-L-proline], a specific irreversible inhibitor, to bovine spleen cathepsin B was elucidated by X-ray crystal...

Crystallization and Preliminary X-Ray Diffraction Study of Recombinant Human Eukaryotic Initiation Factor-4E (1996)

Morino, Shigenobu, Tomoo, Koji, Nishi, Noriko, Okabe, Koumei, Doi, Mitsunobu, Ishida, Toshimasa, ...

Recombinant human eukaryotic initiation factor-4E (eIF-4E), purified by m7GTP-Sepha-rose 4B affinity chromatography, was used for crystallization. After concentration of the eIF-4E protein (7...

Crystallization and Preliminary X-Ray Study of Agkistrodon halys blomhoffii Phospholipase A2 Complexed with a Specific Inhibitor (1993)

Tomoo, Koji, Fujiii, Shinobu, Ishida, Toshimasa, Inoue, Masaloshi, Ikeda, Kiyoshi, Samejima, Yuji, ...

Phospholipase A2 from the venom of Agkistrodon halys blomhoffii has been crystallized as a complex with a specific inhibitor, (iS)-2-dodecanoyl-amino-3-hexanol-l-phosphoglycol. The complex crystals...

Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region.

Tomoo, Koji, Shen, Xu, Okabe, Koumei, Nozoe, Yoshiaki, Fukuhara, Shoichi, Morino, Shigenobu, ...

The crystal structures of the full-length human eukaryotic initiation factor (eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine 5'-triphosphate (m(7)GTP) and...

Crystal structures of 7-methylguanosine 5'-triphosphate (m(7)GTP)- and P(1)-7-methylguanosine-P(3)-adenosine-5',5'-triphosphate (m(7)GpppA)-bound human full-length eukaryotic initiation factor 4E: biological importance of the C-terminal flexible region.

Tomoo, Koji, Shen, Xu, Okabe, Koumei, Nozoe, Yoshiaki, Fukuhara, Shoichi, Morino, Shigenobu, ...

The crystal structures of the full-length human eukaryotic initiation factor (eIF) 4E complexed with two mRNA cap analogues [7-methylguanosine 5'-triphosphate (m(7)GTP) and...

Crystallization and preliminary X-ray crystallographic analysis of BxlE, a xylobiose transporter from Streptomyces thermoviolaceus OPC-520

Seike, Kiho, Sato, Junji, Tomoo, Koji, Ishida, Toshimasa, Yamano, Akihito, Ikenishi, Sadao, ...

To clarify the structural basis of sugar binding by BxlE at the atomic level, recombinant BxlE was crystallized using the hanging-drop vapour-diffusion method at 290 K.