Suzuki, Yohey, Kopp, Robert E., Kogure, Toshihiro, Suga, Akinobu, Takai, Ken, Tsuchida, Shinji, ...
A gastropod from a deep-sea hydrothermal field at the Rodriguez triple junction, Indian Ocean, has scale-shaped structures, called sclerites, mineralized with iron sulfides on its foot. No other...
Nakagawa, Yoshinori, Takada, Masayasu, Ogawa, Koichi, Hatada, Yuji, Horikoshi, Koki
A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into γ-cyclodextrin (γ-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase...
Nakagawa, Yoshinori, Takada, Masayasu, Ogawa, Koichi, Hatada, Yuji, Horikoshi, Koki
A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into γ-cyclodextrin (γ-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase...
Nakagawa, Yoshinori, Takada, Masayasu, Ogawa, Koichi, Hatada, Yuji, Horikoshi, Koki
A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into γ-cyclodextrin (γ-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase...
Moriya Ohkuma, Kuniyo Ohtoko, Toshiya Iida, Mitsunori Tokura, Shigeharu Moriya, Ron Usami, ...
The phylogenetic diversity of parabasalian flagellates was examined based on the sequences of small subunit ribosomal RNA genes amplified directly from the mixed population of flagellates in the...
Takami, Hideto, Nakasone, Kaoru, Takaki, Yoshihiro, Maeno, Go, Sasaki, Rumie, Masui, Noriaki, ...
The 4 202 353 bp genome of the alkaliphilic bacterium Bacillus halodurans C-125 contains 4066 predicted protein coding sequences (CDSs), 2141 (52.7%) of which have functional assignments, 1182...
Kaneko, Hiroyuki, Obuchi, Kaoru, Horikoshi, Koki
We investigated the properties of membrane lipids of barotolerant Pseudomonas sp. BT1 by differential scanning calorimetry and spectrophotometry using a system equipped with a hydrostatic pressure...
Kawarabayasi, Yutaka, Sawada, Mituhiro, Horikawa, Hiroshi, Haikawa, Yuji, Hino, Yumi, Yamamoto, Saori, ...
Kawarabayasi, Yutaka, Sawada, Mituhiro, Horikawa, Hiroshi, Haikawa, Yuji, Hino, Yumi, Yamamoto, Saori, ...
The complete sequence of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3, has been determined by assembling the sequences of the physical map-based contigs of fosmid...
Kato, Chiaki, Smorawinska, Maria, Li, Lina, Horikoshi, Koki
Aspartate β-D-semialdehyde dehydrogenase genes (asd) were cloned and sequenced from a deep-sea-adapted strictly barophilic bacterium, Shewanella sp. strain DB6705, anda moderately barophilic...
Kato, Chiaki, Tamegai, Hideyuki, Ikegami, Akihiko, Usami, Ron, Horikoshi, Koki
Escherichia coli strain JD518, a cydD-deficient mutant, displayed temperature-sensitive and pressure-sensitive growth. The defective cydD gene in this strain was complemented by open reading frame 3...
Kobayashi, Tetsuo, Higuchi, Sadaharu, Kimura, Kinuko, Kudo, Toshiaki, Horikoshi, Koki
Thermococcus profundus, a hyperthermophilic archaeon, did not exhibit detectable glutamine synthetase activity, although the organism possessed an extraordinarily high level of glutamate...
Horikoshi, Koki, Grant, William D
Incluye bibliografía e índice
Nakano, Yoshio, Kato, Chiaki, Tanaka, Eiichl, Kimura, Kinuko, Horikoshi, Koki
We have determined the complete nucleotide sequence of a 2.4 kb chromosomal EcoT22l-NspW fragment, containing the Bacillus cereus glnA gene (structural gene of glutamine synthetase). The deduced...
Cloning of Leucine Genes of Alkalophilic Bacillus No. 221 in E. coli and B. subtilis (1984)
HONDA, Hiroshi, KATO, Chiaki, KUDO, Toshiaki, HORIKOSHI, Koki
The leucine genes of alkalophilic Bacillus No. 221 were cloned into the HindIII sites of pBR322 and pGR71, and recombinant plasmids pHK101 and pHK111 were constructed. The cloned 7.2 kb DNA...
Alkalophilic microorganisms : a new microbial world / Koki Horikoshi and Teruhiko Akiba (1982)
Horikoshi, Koki, Akiba, Teruhiko
Incluye bibliografía e índice
Sodium-Ion Stimulated Amino Acid Uptake in Membrane Vesicles of Alkalophilic Bacillus No. 8-1 (1980)
KITADA, Makio, HORIKOSHI, Koki
Membrane vesicles were isolated from alkalophilic Bacillus No. 8–1, and the active transport of amino acids was studied. The transport of amino acids was dependent upon substrate oxidation and the...
KITADA, Makio, HORIKOSHI, Koki
Alkalophilic Bacillus No. 8-1 and No. 10A-2, isolated on alkaline media containing NaHCO3, and K2CO3, respectively, required Na+ specifically for the uptake of α-aminoisobutyric acid (AIB) into the...
Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae
Abe, Fumiyoshi, Horikoshi, Koki
Hydrostatic pressure in the range of 15 to 25 MPa was found to cause arrest of the cell cycle in G1 phase in an exponentially growing culture of Saccharomyces cerevisiae, whereas a pressure of 50 MPa...
Noda, Satoko, Ohkuma, Moriya, Usami, Ron, Horikoshi, Koki, Kudo, Toshiaki
Expression of the nitrogen fixation gene, nifH, in the gut of the termite Neotermes koshunensis was characterized without cultivation. nifH cDNA was directly amplified from mRNA of the mixed...
Molecular phylogenetic analysis of a naturally occurring microbial community in a deep-subsurface geothermal environment indicated that the phylogenetic diversity of the microbial population in the...
We describe a rapid, reproducible, and sensitive method for detection and quantification of archaea in naturally occurring microbial communities. A domain-specific PCR primer set and a...
Distribution of Archaea in a Black Smoker Chimney Structure
Takai, Ken, Komatsu, Tetsushi, Inagaki, Fumio, Horikoshi, Koki
Archaeal community structures in microhabitats in a deep-sea hydrothermal vent chimney structure were evaluated through the combined use of culture-independent molecular analyses and enrichment...
Novel Toluene Elimination System in a Toluene-Tolerant Microorganism
Kobayashi, Hideki, Uematsu, Katsuyuki, Hirayama, Hisako, Horikoshi, Koki
In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs...
Alkaliphiles: Some Applications of Their Products for Biotechnology
The term “alkaliphile” is used for microorganisms that grow optimally or very well at pH values above 9 but cannot grow or grow only slowly at the near-neutral pH value of 6.5. Alkaliphiles...
Yamada, Mitsunori, Nakasone, Kaoru, Tamegai, Hideyuki, Kato, Chiaki, Usami, Ron, Horikoshi, Koki
Two c-type cytochromes from the soluble fraction of a deep-sea moderately piezophilic bacterium, Shewanella violacea, were purified and characterized, and the genes coding for these cytochromes were...
Outer Membrane Changes in a Toluene-Sensitive Mutant of Toluene-Tolerant Pseudomonas putida IH-2000
Kobayashi, Hideki, Takami, Hideto, Hirayama, Hisako, Kobata, Kuniko, Usami, Ron, Horikoshi, Koki
We isolated a toluene-sensitive mutant, named mutant No. 32, which showed unchanged antibiotic resistance levels, from toluene-tolerant Pseudomonas putida IH-2000 by transposon mutagenesis with Tn5....
Kato, Chiaki, Li, Lina, Nogi, Yuichi, Nakamura, Yuka, Tamaoka, Jin, Horikoshi, Koki
Two strains of obligately barophilic bacteria were isolated from a sample of the world’s deepest sediment, which was obtained by the unmanned deep-sea submersible Kaiko in the Mariana Trench,...
Takami, Hideto, Nakasone, Kaoru, Takaki, Yoshihiro, Maeno, Go, Sasaki, Rumie, Masui, Noriaki, ...
The 4 202 353 bp genome of the alkaliphilic bacterium Bacillus halodurans C-125 contains 4066 predicted protein coding sequences (CDSs), 2141 (52.7%) of which have functional assignments, 1182 (29%)...
Takai, Ken, Hirayama, Hisako, Sakihama, Yuri, Inagaki, Fumio, Yamato, Yu, Horikoshi, Koki
Culture-dependent and -independent techniques were combined to characterize the physiological properties and the ecological impacts of culture-resistant phylotypes of thermophiles within the order...
Pseudomonas putida Which Can Grow in the Presence of Toluene
Inoue, Akira, Yamamoto, Mami, Horikoshi, Koki
A Pseudomonas putida strain able to grow in the presence of more than 50% toluene was isolated from soil. The strain was tolerant of other toxic solvents, including aliphatic hydrocarbons, alicyclic...
Aono, Rikizo, Doukyu, Noriyuki, Kobayashi, Hideki, Nakajima, Harushi, Horikoshi, Koki
Pseudomonas sp. strain ST-200, which is capable of conversion of cholesterol, was isolated from humus soil. This organism effectively modified cholesterol dissolved in an organic solvent by...
Characterization of a Protease from a Psychrotroph, Pseudomonas fluorescens 114
Hamamoto, Tetsuo, Kaneda, Motohiro, Horikoshi, Koki, Kudo, Toshiaki
A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0°C and optimally at 20°C. The bacterium produced a protease with a molecular...
Isolation of Bacteria Which Can Grow at Both High pH and Low Temperature
Kimura, Takuhei, Horikoshi, Koki
Fifty bacterial strains able to grow at pH 10 and 0°C were isolated from soils, and growth characteristics of three selected strains were investigated. Strain 207, which showed the best growth rate...
Mannitol and Mannitol Dehydrogenases in Conidia of Aspergillus oryzae
Horikoshi, Koki, Iida, Shigeji, Ikeda, Yonosuke
Horikoshi, Koki (The Institute of Physical and Chemical Research, Tokyo, Japan), Shigeji Iida, and Yonosuke Ikeda. Mannitol and mannitol dehydrogenases in conidia of Aspergillus oryzae. J. Bacteriol....
Inagaki, Fumio, Suzuki, Masae, Takai, Ken, Oida, Hanako, Sakamoto, Tatsuhiko, Aoki, Kaori, ...
Microbial communities from a subseafloor sediment core from the southwestern Sea of Okhotsk were evaluated by performing both cultivation-dependent and cultivation-independent (molecular) analyses....
Trehalase in Conidia of Aspergillus oryzae
Horikoshi, Koki, Ikeda, Yonosuke
Horikoshi, Koki (The Institute of Physical and Chemical Research, Bunkyo-ku, Tokyo, Japan), and Yonosuke Ikeda. Trehalase in conidia of Aspergillus oryzae. J. Bacteriol. 91:1883–1887....
Takai, Ken, Oida, Hanako, Suzuki, Yohey, Hirayama, Hisako, Nakagawa, Satoshi, Nunoura, Takuro, ...
Distribution profiles of marine crenarchaeota group I in the vicinity of deep-sea hydrothermal systems were mapped with culture-independent molecular techniques. Planktonic samples were obtained from...
Inagaki, Fumio, Tsunogai, Urumu, Suzuki, Masae, Kosaka, Ayako, Machiyama, Hideaki, Takai, Ken, ...
Samples from three submerged sites (MC, a core obtained in the methane seep area; MR, a reference core obtained at a distance from the methane seep; and HC, a gas-bubbling carbonate sample) at the...
Suzuki, Yohey, Sasaki, Takenori, Suzuki, Masae, Nogi, Yuichi, Miwa, Tetsuya, Takai, Ken, ...
The hydrothermal-vent gastropod Alviniconcha aff. hessleri from the Kairei hydrothermal field on the Central Indian Ridge houses bacterium-like cells internally in its greatly enlarged gill. A single...
Takai, Ken, Campbell, Barbara J., Cary, S. Craig, Suzuki, Masae, Oida, Hanako, Nunoura, Takuro, ...
The carbon and energy metabolisms of a variety of cultured chemolithoautotrophic Epsilonproteobacteria from deep-sea hydrothermal environments were characterized by both enzymatic and genetic...
Suzuki, Yohey, Kojima, Shigeaki, Sasaki, Takenori, Suzuki, Masae, Utsumi, Takashi, Watanabe, Hiromi, ...
Hydrothermal vent gastropods of the genus Alviniconcha are unique among metazoans in their ability to derive their nutrition from chemoautotrophic γ- and ɛ-proteobacterial endosymbionts. Although...
Inagaki, Fumio, Nunoura, Takuro, Nakagawa, Satoshi, Teske, Andreas, Lever, Mark, Lauer, Antje, ...
The deep subseafloor biosphere is among the least-understood habitats on Earth, even though the huge microbial biomass therein plays an important role for potential long-term controls on global...
Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae
Abe, Fumiyoshi, Horikoshi, Koki
Hydrostatic pressure in the range of 15 to 25 MPa was found to cause arrest of the cell cycle in G1 phase in an exponentially growing culture of Saccharomyces cerevisiae, whereas a pressure of 50 MPa...
Noda, Satoko, Ohkuma, Moriya, Usami, Ron, Horikoshi, Koki, Kudo, Toshiaki
Expression of the nitrogen fixation gene, nifH, in the gut of the termite Neotermes koshunensis was characterized without cultivation. nifH cDNA was directly amplified from mRNA of the mixed...
Molecular phylogenetic analysis of a naturally occurring microbial community in a deep-subsurface geothermal environment indicated that the phylogenetic diversity of the microbial population in the...
We describe a rapid, reproducible, and sensitive method for detection and quantification of archaea in naturally occurring microbial communities. A domain-specific PCR primer set and a...
Distribution of Archaea in a Black Smoker Chimney Structure
Takai, Ken, Komatsu, Tetsushi, Inagaki, Fumio, Horikoshi, Koki
Archaeal community structures in microhabitats in a deep-sea hydrothermal vent chimney structure were evaluated through the combined use of culture-independent molecular analyses and enrichment...
Novel Toluene Elimination System in a Toluene-Tolerant Microorganism
Kobayashi, Hideki, Uematsu, Katsuyuki, Hirayama, Hisako, Horikoshi, Koki
In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs...
Alkaliphiles: Some Applications of Their Products for Biotechnology
The term “alkaliphile” is used for microorganisms that grow optimally or very well at pH values above 9 but cannot grow or grow only slowly at the near-neutral pH value of 6.5. Alkaliphiles...
Yamada, Mitsunori, Nakasone, Kaoru, Tamegai, Hideyuki, Kato, Chiaki, Usami, Ron, Horikoshi, Koki
Two c-type cytochromes from the soluble fraction of a deep-sea moderately piezophilic bacterium, Shewanella violacea, were purified and characterized, and the genes coding for these cytochromes were...
Outer Membrane Changes in a Toluene-Sensitive Mutant of Toluene-Tolerant Pseudomonas putida IH-2000
Kobayashi, Hideki, Takami, Hideto, Hirayama, Hisako, Kobata, Kuniko, Usami, Ron, Horikoshi, Koki
We isolated a toluene-sensitive mutant, named mutant No. 32, which showed unchanged antibiotic resistance levels, from toluene-tolerant Pseudomonas putida IH-2000 by transposon mutagenesis with Tn5....
Kato, Chiaki, Li, Lina, Nogi, Yuichi, Nakamura, Yuka, Tamaoka, Jin, Horikoshi, Koki
Two strains of obligately barophilic bacteria were isolated from a sample of the world’s deepest sediment, which was obtained by the unmanned deep-sea submersible Kaiko in the Mariana Trench,...
Takami, Hideto, Nakasone, Kaoru, Takaki, Yoshihiro, Maeno, Go, Sasaki, Rumie, Masui, Noriaki, ...
The 4 202 353 bp genome of the alkaliphilic bacterium Bacillus halodurans C-125 contains 4066 predicted protein coding sequences (CDSs), 2141 (52.7%) of which have functional assignments, 1182 (29%)...
Takai, Ken, Hirayama, Hisako, Sakihama, Yuri, Inagaki, Fumio, Yamato, Yu, Horikoshi, Koki
Culture-dependent and -independent techniques were combined to characterize the physiological properties and the ecological impacts of culture-resistant phylotypes of thermophiles within the order...
Pseudomonas putida Which Can Grow in the Presence of Toluene
Inoue, Akira, Yamamoto, Mami, Horikoshi, Koki
A Pseudomonas putida strain able to grow in the presence of more than 50% toluene was isolated from soil. The strain was tolerant of other toxic solvents, including aliphatic hydrocarbons, alicyclic...
Aono, Rikizo, Doukyu, Noriyuki, Kobayashi, Hideki, Nakajima, Harushi, Horikoshi, Koki
Pseudomonas sp. strain ST-200, which is capable of conversion of cholesterol, was isolated from humus soil. This organism effectively modified cholesterol dissolved in an organic solvent by...
Characterization of a Protease from a Psychrotroph, Pseudomonas fluorescens 114
Hamamoto, Tetsuo, Kaneda, Motohiro, Horikoshi, Koki, Kudo, Toshiaki
A psychrotrophic bacterium isolated from river sediment was identified as Pseudomonas fluorescens 114. It grew at 0°C and optimally at 20°C. The bacterium produced a protease with a molecular...
Isolation of Bacteria Which Can Grow at Both High pH and Low Temperature
Kimura, Takuhei, Horikoshi, Koki
Fifty bacterial strains able to grow at pH 10 and 0°C were isolated from soils, and growth characteristics of three selected strains were investigated. Strain 207, which showed the best growth rate...
Mannitol and Mannitol Dehydrogenases in Conidia of Aspergillus oryzae
Horikoshi, Koki, Iida, Shigeji, Ikeda, Yonosuke
Horikoshi, Koki (The Institute of Physical and Chemical Research, Tokyo, Japan), Shigeji Iida, and Yonosuke Ikeda. Mannitol and mannitol dehydrogenases in conidia of Aspergillus oryzae. J. Bacteriol....
Inagaki, Fumio, Suzuki, Masae, Takai, Ken, Oida, Hanako, Sakamoto, Tatsuhiko, Aoki, Kaori, ...
Microbial communities from a subseafloor sediment core from the southwestern Sea of Okhotsk were evaluated by performing both cultivation-dependent and cultivation-independent (molecular) analyses....
Trehalase in Conidia of Aspergillus oryzae
Horikoshi, Koki, Ikeda, Yonosuke
Horikoshi, Koki (The Institute of Physical and Chemical Research, Bunkyo-ku, Tokyo, Japan), and Yonosuke Ikeda. Trehalase in conidia of Aspergillus oryzae. J. Bacteriol. 91:1883–1887....
Takai, Ken, Oida, Hanako, Suzuki, Yohey, Hirayama, Hisako, Nakagawa, Satoshi, Nunoura, Takuro, ...
Distribution profiles of marine crenarchaeota group I in the vicinity of deep-sea hydrothermal systems were mapped with culture-independent molecular techniques. Planktonic samples were obtained from...
Inagaki, Fumio, Tsunogai, Urumu, Suzuki, Masae, Kosaka, Ayako, Machiyama, Hideaki, Takai, Ken, ...
Samples from three submerged sites (MC, a core obtained in the methane seep area; MR, a reference core obtained at a distance from the methane seep; and HC, a gas-bubbling carbonate sample) at the...
Suzuki, Yohey, Sasaki, Takenori, Suzuki, Masae, Nogi, Yuichi, Miwa, Tetsuya, Takai, Ken, ...
The hydrothermal-vent gastropod Alviniconcha aff. hessleri from the Kairei hydrothermal field on the Central Indian Ridge houses bacterium-like cells internally in its greatly enlarged gill. A single...
Takai, Ken, Campbell, Barbara J., Cary, S. Craig, Suzuki, Masae, Oida, Hanako, Nunoura, Takuro, ...
The carbon and energy metabolisms of a variety of cultured chemolithoautotrophic Epsilonproteobacteria from deep-sea hydrothermal environments were characterized by both enzymatic and genetic...
Suzuki, Yohey, Kojima, Shigeaki, Sasaki, Takenori, Suzuki, Masae, Utsumi, Takashi, Watanabe, Hiromi, ...
Hydrothermal vent gastropods of the genus Alviniconcha are unique among metazoans in their ability to derive their nutrition from chemoautotrophic γ- and ɛ-proteobacterial endosymbionts. Although...
Inagaki, Fumio, Nunoura, Takuro, Nakagawa, Satoshi, Teske, Andreas, Lever, Mark, Lauer, Antje, ...
The deep subseafloor biosphere is among the least-understood habitats on Earth, even though the huge microbial biomass therein plays an important role for potential long-term controls on global...
Microbial Community in Black Rust Exposed to Hot Ridge Flank Crustal Fluids▿ †
Nakagawa, Satoshi, Inagaki, Fumio, Suzuki, Yohey, Steinsbu, Bjørn Olav, Lever, Mark Alexander, Takai, Ken, ...
During Integrated Ocean Drilling Program Expedition 301, we obtained a sample of black rust from a circulation obviation retrofit kit (CORK) observatory at a borehole on the eastern flank of Juan de...
Microbial community in a sediment-hosted CO2 lake of the southern Okinawa Trough hydrothermal system
Inagaki, Fumio, Kuypers, Marcel M. M., Tsunogai, Urumu, Ishibashi, Jun-ichiro, Nakamura, Ko-ichi, Treude, Tina, ...
Increasing levels of CO2 in the atmosphere are expected to cause climatic change with negative effects on the earth's ecosystems and human society. Consequently, a variety of CO2 disposal options are...
Deep-sea vent ε-proteobacterial genomes provide insights into emergence of pathogens
Nakagawa, Satoshi, Takaki, Yoshihiro, Shimamura, Shigeru, Reysenbach, Anna-Louise, Takai, Ken, Horikoshi, Koki
Deep-sea vents are the light-independent, highly productive ecosystems driven primarily by chemolithoautotrophic microorganisms, in particular by ε-Proteobacteria phylogenetically related to...
Hirayama, Hisako, Sunamura, Michinari, Takai, Ken, Nunoura, Takuro, Noguchi, Takuro, Oida, Hanako, ...
Microbial communities in a shallow submarine hydrothermal system near Taketomi Island, Japan, were investigated using cultivation-based and molecular techniques. The main hydrothermal activity...
Takai, Ken, Nakamura, Kentaro, Toki, Tomohiro, Tsunogai, Urumu, Miyazaki, Masayuki, Miyazaki, Junichi, ...
We have developed a technique for cultivation of chemolithoautotrophs under high hydrostatic pressures that is successfully applicable to various types of deep-sea chemolithoautotrophs, including...
Versatile Solidified Nanofibrous Cellulose-Containing Media for Growth of Extremophiles▿
Tsudome, Mikiko, Deguchi, Shigeru, Tsujii, Kaoru, Ito, Susumu, Horikoshi, Koki
Solidified media that employ a porous matrix of nanofibrous cellulose are described. The physicochemical stability of the porous structure allows the development of solidified media that can support...