Kouji Matsushima

Massive transcriptional start site analysis of human genes in hypoxia cells (2009)

Tsuchihara, Katsuya, Suzuki, Yutaka, Wakaguri, Hiroyuki, Irie, Takuma, Tanimoto, Kousuke, Hashimoto, Shin-ichi, ...

Combining our full-length cDNA method and the massively parallel sequencing technology, we developed a simple method to collect precise positional information of transcriptional start sites (TSSs)...

MachiBase: a Drosophila melanogaster 5'-end mRNA transcription database (2009)

Ahsan, Budrul, Saito, Taro L., Hashimoto, Shin-ichi, Muramatsu, Keigo, Tsuda, Manabu, Sasaki, Atsushi, ...

MachiBase (http://machibase.gi.k.u-tokyo.ac.jp/) provides a comprehensive and freely accessible resource regarding Drosophila melanogaster 5′-end mRNA transcription at different developmental...

UTGB/medaka: genomic resource database for medaka biology (2008)

Ahsan, Budrul, Kobayashi, Daisuke, Yamada, Tomoyuki, Kasahara, Masahiro, Sasaki, Shin, Saito, Taro L., ...

Medaka (Oryzias latipes) is a small egg-laying freshwater teleost native to East Asia that has become an excellent model system for developmental genetics and evolutionary biology. The draft medaka...

CD4+CD25+ regulatory T cells in the small intestinal lamina propria show an effector/memory phenotype (2008)

Guo, Zijin, Jang, Myoung Ho, Otani, Kazuhiro, Bai, Zhongbin, Umemoto, Eiji, Matsumoto, Masanori, ...

CD4+CD25+ regulatory T cells (Tregs) have been implicated in the suppression of pathogenic responses to both self- and non-self-antigens in the intestine. However, their precise properties and...

Maintenance of memory CD8+ T cell diversity and proliferative potential by a primary response upon re-challenge (2007)

Kurachi, Makoto, Kakimi, Kazuhiro, Ueha, Satoshi, Matsushima, Kouji

Memory CD8+ T cells generated during an immune response are long lived and self-renewing, offering enhanced host protection against re-infection. However, how an antigen-specific population of memory...

Synovial autoreactive T cells in rheumatoid arthritis resist IDO-mediated inhibition (2006)

Ji, Fang, Wang, Yuan, Zhang, Yi, Zhang, Jingwu Z, ...

A hallmark of T cell-mediated autoimmunity is the persistence of autoreactive T cells. However, it remains to elucidate the manner in which synovial T cells are sustained in patients with rheumatoid...

Trans-basement membrane migration of human basophils: role of matrix metalloproteinase-9 (2006)

Suzukawa, Maho, Komiya, Akiko, Iikura, Motoyasu, Nagase, Hiroyuki, Yoshimura-Uchiyama, Chitose, Yamada, Hirokazu, ...

In allergic disorders, basophils migrate from the blood stream to inflamed tissue sites. Since trans-basement membrane migration is an important step for local basophil accumulation, we performed a...

Maintenance of memory CD8+ T cell diversity and proliferative potential by a primary response upon re-challenge (2006)

Kurachi, Makoto, Kakimi, Kazuhiro, Ueha, Satoshi, Matsushima, Kouji

Memory CD8+ T cells generated during an immune response are long lived and self-renewing, offering enhanced host protection against re-infection. However, how an antigen-specific population of memory...

Trans-basement membrane migration of human basophils: role of matrix metalloproteinase-9 (2006)

Suzukawa, Maho, Komiya, Akiko, Iikura, Motoyasu, Nagase, Hiroyuki, Yoshimura-Uchiyama, Chitose, Yamada, Hirokazu, ...

In allergic disorders, basophils migrate from the blood stream to inflamed tissue sites. Since trans-basement membrane migration is an important step for local basophil accumulation, we performed a...

5'SAGE: 5'-end Serial Analysis of Gene Expression database (2005)

Kasai, Yasuhiro, Hashimoto, Shin-ichi, Yamada, Tomoyuki, Sese, Jun, Sugano, Sumio, Matsushima, Kouji, ...

To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5′ end Serial Analysis of Gene Expression (SAGE) was developed...

The simultaneous blockade of chemokine receptors CCR2, CCR5 and CXCR3 by a non-peptide chemokine receptor antagonist protects mice from dextran sodium sulfate-mediated colitis (2005)

Tokuyama, Hirotake, Ueha, Satoshi, Kurachi, Makoto, Matsushima, Kouji, Moriyasu, Fuminori, Blumberg, Richard S., ...

Chemokine receptors CCR2, CCR5 and CXCR3 are involved in the regulation of macrophage- and T cell-mediated immune responses and in the migration and activation of these cells. In order to determine...

The simultaneous blockade of chemokine receptors CCR2, CCR5 and CXCR3 by a non-peptide chemokine receptor antagonist protects mice from dextran sodium sulfate-mediated colitis (2005)

Tokuyama, Hirotake, Ueha, Satoshi, Kurachi, Makoto, Matsushima, Kouji, Moriyasu, Fuminori, Blumberg, Richard S., ...

Chemokine receptors CCR2, CCR5 and CXCR3 are involved in the regulation of macrophage- and T cell-mediated immune responses and in the migration and activation of these cells. In order to determine...

doi:10.1093/nar/gki085 5 0 SAGE: 5 0-end Serial Analysis of Gene Expression database (2004)

Yasuhiro Kasai, Shin-ichi Hashimoto, Tomoyuki Yamada, Jun Sese, Sumio Sugano, Kouji Matsushima, ...

To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5 0 end Serial Analysis of Gene Expression (SAGE) was developed...

Endocrine Disruptors (Environmental Estrogens) Enhance Autoantibody Production by B1 Cells (2004)

Yurino, Hideaki, Ishikawa, Sho, Sato, Taku, Akadegawa, Kenji, Ito, Toshihiro, Ueha, Satoshi, ...

Accumulating data suggest that endocrine disruptors affect not only the reproductive system, but also the immune system. We demonstrate here that endocrine disruptors including diethylstilbestrol...

Endocrine Disruptors (Environmental Estrogens) Enhance Autoantibody Production by B1 Cells (2004)

Yurino, Hideaki, Ishikawa, Sho, Sato, Taku, Akadegawa, Kenji, Ito, Toshihiro, Ueha, Satoshi, ...

Accumulating data suggest that endocrine disruptors affect not only the reproductive system, but also the immune system. We demonstrate here that endocrine disruptors including diethylstilbestrol...

Mobilization of Dendritic Cell Precursors Into the Circulation by Administration of MIP-1 in Mice (2004)

Zhang, Yanyun, Yoneyama, Hiroyuki, Wang, Yong, Ishikawa, Sho, Hashimoto, Shin-ichi, Gao, Ji-Liang, ...

Background: Dendritic cells (DCs) play a central role in immune responses and may be useful adjuvants for tumor vaccine therapy. We previously reported that F4/80–B220–CD11c+ DC precursors...

Evidence for recruitment of plasmacytoid dendritic cell precursors to inflamed lymph nodes through high endothelial venules (2004)

Yoneyama, Hiroyuki, Matsuno, Kenjiro, Zhang, Yanyun, Nishiwaki, Tetsu, Kitabatake, Masahiro, Ueha, Satoshi, ...

Recruitment of dendritic cells (DCs) to lymph nodes (LNs) is pivotal to the establishment of immune response. Whereas DCs have been proven to undergo afferent lymphatic pathway to enter LNs from...

Analysis of mRNA With Microsomal Fractionation Using a SAGE-Based DNA Microarray System Facilitates Identification of the Genes Encoding Secretory Proteins (2003)

Toyoda, Nobuaki, Nagai, Shigenori, Terashima, Yuya, Motomura, Kazushi, Haino, Makoto, Hashimoto, Shin-ichi, ...

In the regulation of host defense responses such as inflammation and immunity, the secretory proteins, including membrane proteins, play central roles. Although many secretory proteins have been...

Analysis of mRNA With Microsomal Fractionation Using a SAGE-Based DNA Microarray System Facilitates Identification of the Genes Encoding Secretory Proteins (2003)

Toyoda, Nobuaki, Nagai, Shigenori, Terashima, Yuya, Motomura, Kazushi, Haino, Makoto, Hashimoto, Shin-ichi, ...

In the regulation of host defense responses such as inflammation and immunity, the secretory proteins, including membrane proteins, play central roles. Although many secretory proteins have been...

Pivotal role of CCL25 (TECK)-CCR9 in the formation of gut cryptopatches and consequent appearance of intestinal intraepithelial T lymphocytes (2002)

Onai, Nobuyuki, Kitabatake, Masahiro, Zhang, Yan-yun, Ishikawa, Hiromichi, Ishikawa, Sho, Matsushima, Kouji

Cryptopatches (CP) are murine gut anatomical sites for generating thymus‐independent intraepithelial T lymphocytes (IEL). However, it remains elusive how lympho‐hematopoietic progenitor...

p38 MAPK phosphorylation and NF-{kappa}B activation in human crescentic glomerulonephritis (2002)

Sakai, Norihiko, Wada, Takashi, Furuichi, Kengo, Iwata, Yasunori, Yoshimoto, Keiichi, Kitagawa, Kiyoki, ...

Background. p38 mitogen‐activated protein kinase (p38 MAPK) followed by the activation of NF‐κB participates in the intracellular signal transduction and production of...

Administration of FR167653, a new anti-inflammatory compound, prevents renal ischaemia/reperfusion injury in mice (2002)

Furuichi, Kengo, Wada, Takashi, Iwata, Yasunori, Sakai, Norihiko, Yoshimoto, Keiichi, Kobayashi, Ken-ichi, ...

Background. Various types of chemokines/cytokines play important roles in ischaemia/reperfusion injury in kidneys. However, the roles of p38 mitogen‐activated protein kinase...

A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1 null mutant mice develop renal lesions mimicking obstructive nephropathy (2002)

Yokoyama, Hitoshi, Wada, Takashi, Kobayashi, Ken-ichi, Kuno, Kouji, Kurihara, Hiroki, Shindo, Takayuki, ...

Background. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)‐1 is distinguished from other a disintegrin and metalloproteinase molecules by the presence of...

Chemokines in renal diseases (2001)

Wada, Takashi, Yukoyama, Hitoshi, Matsushima, Kouji, Kobayashi, Kenichi, 和田, 隆志

The interaction of activated leukocytes and renal resident cells is thought to play a crucial role in the pathogenesis of renal diseases. Recent investigations of the pathophysiological roles of...

Comprehensive gene expression profile of human activated Th1- and Th2-polarized cells (2001)

Nagai, Shigenori, Hashimoto, Shin-ichi, Yamashita, Taro, Toyoda, Nobuaki, Satoh, Taku, Suzuki, Takuji, ...

In response to antigen stimulation, Th cells differentiate into two types of effector cells, Th1 and Th2. Th1 cells predominantly mediate cellular immunity, whereas Th2 cells induce humoral allergic...

Redistribution of selectin counter-ligands induced by cytokines (2000)

Nagata, Kisaburo, Tsuji, Tsutomu, Matsushima, Kouji, Hanai, Nobuo, Irimura, Tatsuro

Soluble recombinant (r) P-selectin and rP-selectin immobilized on plastic surfaces were tested for their capacity to activate neutrophils to produce superoxide anion. Soluble rP-selectin was...

IL-1 Induces Expression of p21WAF1 Independently of p53 in High-Passage Human Embryonic Fibroblasts WI38 (2000)

Osawa, Yoshiaki, Hachiya, Misao, Araki, Shun-ichi, Kusama, Tomoko, Matsushima, Kouji, Aoki, Yoshiro, ...

We tested the effect of IL-1 on the expression of p21WAF1 in human embryonic fibroblasts WI38. Exposure to IL-1 caused induction of p21WAF1 protein in high-passage WI38 cells but not in early-passage...

Selective recruitment of CCR4-bearing Th2 cells toward antigen-presenting cells by the CC chemokines thymus and activation-regulated chemokine and macrophage-derived chemokine (1999)

Imai, Toshio, Nagira, Morio, Takagi, Shin, Kakizaki, Mayumi, Nishimura, Miyuki, Wang, Jianbin, ...

Helper T cells are classified into Th1 and Th2 subsets based on their profiles of cytokine production. Th1 cells are involved in cell-mediated immunity, whereas Th2 cells induce humoral responses....

Th1-derived cytokine IFN-{gamma} is a potent inhibitor of eotaxin synthesis in vitro (1999)

Miyamasu, Misato, Yamaguchi, Masao, Nakajima, Toshiharu, Misaki, Yoshikata, Morita, Yutaka, Matsushima, Kouji, ...

Eotaxin potentially plays an integral role in tissue eosinophilia. Inasmuch as Th2-derived cytokine IL-4 has been shown to stimulate eotaxin generation, we investigated here the effect of Th1-derived...

Prevention of endotoxin shock by an antibody against leukocyte integrin {beta}2through inhibiting production and action of TNF (1995)

Watanabe, Shun-ichi, Mukaida, Naofumi, Ikeda, Naoki, Akiyama, Mariko, Harada, Akihisa, Nakanishi, Isao, ...

Septic shock remains a serious disorder associated with high mortality. Accumulating evidence indicates that TNF is a major and essential mediator of endotoxin shock. We report here that...

Acid sphingomyelinase is not essential for the IL-1 and tumor necrosis factor receptor signaling pathway leading to NFkB activation (1994)

Kuno, Kouji, Sukegawa, Kazuko, Ishikawa, Yuji, Oril, Tadao, Matsushima, Kouji

A recent report has suggested that tumor necrosis factor (TNF) utilizes acid sphingomyelinase (SMase) pathway to activate NFkB (Schutze et al. 1992. Cell 71:765). To directly investigate the role of...

Molecular cloning of cDNAs encoding a LD78 receptor and putative leukocyte chemotactic peptide receptors (1993)

Nomura, Hideki, Nielsen, Bent W., Matsushima, Kouji, Arai, K.

Several cDNA clones encoding receptors for leukocyte chemoattractants, including IL-8, C5a, N-formyl peptides (FP), and platelet-activating factor, have been isolated in the past 3 years. The primary...

Expression of recombinant rabbit IL-8 in Escherichia coli and establishment of the essential involvement of IL-8 in recruiting neutrophils into lipopolysaccharide-induced inflammatory site of rabbit skin (1993)

Harada, Akihisa, Sekido, Nobuaki, Kuno, Kouji, Aklyama, Mariko, Kasahara, Tadashi, Nakanishi, Isao, ...

In order to establish the pathophyslological roles of IL-8, rabbit IL-8 was expressed in Escherichia coli and purified to homogeneity by sequential chromatography on heparin agarose, CM-HPLC, and...

Calcium-Dependent Binding of Phosphorylated Human Pre Interleukin 1{alpha} to Phospholipids (1990)

Kobayashi, Yoshiro, Oppenheim, Joost J., Matsushima, Kouji

The effect of phosphorylation of pre interleukin la (IL la) on its association with various phospholipids was investigated. We prepared genetically engineered truncated human pre IL la (residues 64...

Production and Characterization of Recombinant Human Neutrophil Chemotactic Factor (1989)

Furuta, Ryuji, Yamagishi, Junichi, Kotani, Hirotada, Sakamoto, Fumiko, Fukui, Toshikazu, Matsui, Yukiharu, ...

A putative mature human neutrophil chemotactic factor (NCF) corresponding to the C-terminal 72 amino acids of its precursor was directly produced in Escherichia coli by recombinant DNA technology....

Molecular cloning and characterization of a murine pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 receptor, a murine homolog of the human immunodeficiency virus 1 entry coreceptor fusin

Nagasawa, Takashi, Nakajima, Toshihiro, Tachibana, Kazunobu, Iizasa, Hisashi, Bleul, Conrad C., Yoshie, Osamu, ...

Pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 (PBSF/SDF-1) is a member of the CXC group of chemokines that is initially identified as a bone marrow stromal cell-derived factor...

Nitrite Reductase from Pseudomonas aeruginosa Released by Antimicrobial Agents and Complement Induces Interleukin-8 Production in Bronchial Epithelial Cells

Sar, Borann, Oishi, Kazunori, Wada, Akihiro, Hirayama, Toshiya, Matsushima, Kouji, Nagatake, Tsuyoshi

We have recently reported that nitrite reductase, a bifunctional enzyme located in the periplasmic space of Pseudomonas aeruginosa, could induce interleukin-8 (IL-8) generation in a variety of...

Essential Role of Transcription Factor Nuclear Factor-κB in Regulation of Interleukin-8 Gene Expression by Nitrite Reductase from Pseudomonas aeruginosa in Respiratory Epithelial Cells

Mori, Naoki, Oishi, Kazunori, Sar, Borann, Mukaida, Naofumi, Nagatake, Tsuyoshi, Matsushima, Kouji, ...

Persistent infection with Pseudomonas aeruginosa increases interleukin-8 (IL-8) levels and causes dense neutrophil infiltrations in the airways of patients with chronic airway diseases. Recently, we...

Impairment of Endotoxin-Induced Macrophage Inflammatory Protein 2 Gene Expression in Alveolar Macrophages in Streptozotocin-Induced Diabetes in Mice

Amano, Hideaki, Yamamoto, Hidefumi, Senba, Masachika, Oishi, Kazunori, Suzuki, Shoichi, Fukushima, Kenichi, ...

To elucidate the mechanism of the high incidence of lower respiratory tract infections in patients with diabetes mellitus, we investigated the kinetics of production of macrophage inflammatory...

Fourteen-Member Macrolides Suppress Interleukin-8 Production but Do Not Promote Apoptosis of Activated Neutrophils

Tsuchihashi, Yoshiko, Oishi, Kazunori, Yoshimine, Hiroyuki, Suzuki, Shoichi, Kumatori, Atsushi, Sunazuka, Toshiaki, ...

A 14-member macrolide was found to inhibit interleukin-8 (IL-8) synthesis in lipopolysaccharide-stimulated neutrophils but did not accelerate apoptosis in activated neutrophils. These data suggest...

Fourteen-Member Macrolides Promote the Phosphatidylserine Receptor-Dependent Phagocytosis of Apoptotic Neutrophils by Alveolar Macrophages

Yamaryo, Takeshi, Oishi, Kazunori, Yoshimine, Hiroyuki, Tsuchihashi, Yoshiko, Matsushima, Kouji, Nagatake, Tsuyoshi

An inflammation of the airway of patients with diffuse panbronchiolitis (DPB), is characterized by dense neutrophil infiltration. Resolution of the inflammation can be achieved by the removal of...

ADAMTS-1: a metalloproteinase-disintegrin essential for normal growth, fertility, and organ morphology and function

Shindo, Takayuki, Kurihara, Hiroki, Kuno, Kouji, Yokoyama, Hitoshi, Wada, Takashi, Kurihara, Yukiko, ...

A disintegrin and metalloproteinase (ADAM) represents a protein family possessing both metalloproteinase and disintegrin domains. ADAMTS-1, an ADAM family member cloned from cachexigenic colon...

Analysis of mRNA With Microsomal Fractionation Using a SAGE-Based DNA Microarray System Facilitates Identification of the Genes Encoding Secretory Proteins

Toyoda , Nobuaki, Nagai, Shigenori, Terashima, Yuya, Motomura, Kazushi, Haino, Makoto, Hashimoto, Shin-ichi, ...

In the regulation of host defense responses such as inflammation and immunity, the secretory proteins, including membrane proteins, play central roles. Although many secretory proteins have been...

A functional granulocyte colony-stimulating factor receptor is required for normal chemoattractant-induced neutrophil activation

Betsuyaku, Tomoko, Liu, Fulu, Senior, Robert M., Haug, Jeffery S., Brown, Eric J., Jones, Samuel L., ...

Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor that is widely used to treat neutropenia. In addition to stimulating polymorphonuclear neutrophil (PMN) production,...

Active participation of CCR5+CD8+ T lymphocytes in the pathogenesis of liver injury in graft-versus-host disease

Murai, Masako, Yoneyama, Hiroyuki, Harada, Akihisa, Yi, Zhang, Vestergaard, Christian, Guo, Baoyu, ...

We examined the molecular pathogenesis of graft-versus-host disease–associated (GVHD-associated) liver injury in mice, focusing on the role of chemokines. At the second week after cell transfer in...

Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis–like lesions

Vestergaard, Christian, Yoneyama, Hiroyuki, Murai, Masako, Nakamura, Kohichiro, Tamaki, Kunihiko, Terashima, Yuya, ...

We have examined the expression of chemokines and their receptors in the atopic dermatitis–like (AD-like) lesions of NC/Nga mice. Such lesions develop when the mice are kept in conventional...

5′SAGE: 5′-end Serial Analysis of Gene Expression database

Kasai, Yasuhiro, Hashimoto, Shin-ichi, Yamada, Tomoyuki, Sese, Jun, Sugano, Sumio, Matsushima, Kouji, ...

To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5′ end Serial Analysis of Gene Expression (SAGE) was developed...

Chemokines in renal diseases

Wada, Takashi, Yukoyama, Hitoshi, Matsushima, Kouji, Kobayashi, Ken-ichi

The interaction of activated leukocytes and renal resident cells is thought to play a crucial role in the pathogenesis of renal diseases. Recent investigations of the pathophysiological roles of...

Molecular cloning and characterization of a murine pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 receptor, a murine homolog of the human immunodeficiency virus 1 entry coreceptor fusin

Nagasawa, Takashi, Nakajima, Toshihiro, Tachibana, Kazunobu, Iizasa, Hisashi, Bleul, Conrad C., Yoshie, Osamu, ...

Pre-B-cell growth-stimulating factor/stromal cell-derived factor 1 (PBSF/SDF-1) is a member of the CXC group of chemokines that is initially identified as a bone marrow stromal cell-derived factor...

Nitrite Reductase from Pseudomonas aeruginosa Released by Antimicrobial Agents and Complement Induces Interleukin-8 Production in Bronchial Epithelial Cells

Sar, Borann, Oishi, Kazunori, Wada, Akihiro, Hirayama, Toshiya, Matsushima, Kouji, Nagatake, Tsuyoshi

We have recently reported that nitrite reductase, a bifunctional enzyme located in the periplasmic space of Pseudomonas aeruginosa, could induce interleukin-8 (IL-8) generation in a variety of...

Essential Role of Transcription Factor Nuclear Factor-κB in Regulation of Interleukin-8 Gene Expression by Nitrite Reductase from Pseudomonas aeruginosa in Respiratory Epithelial Cells

Mori, Naoki, Oishi, Kazunori, Sar, Borann, Mukaida, Naofumi, Nagatake, Tsuyoshi, Matsushima, Kouji, ...

Persistent infection with Pseudomonas aeruginosa increases interleukin-8 (IL-8) levels and causes dense neutrophil infiltrations in the airways of patients with chronic airway diseases. Recently, we...

Impairment of Endotoxin-Induced Macrophage Inflammatory Protein 2 Gene Expression in Alveolar Macrophages in Streptozotocin-Induced Diabetes in Mice

Amano, Hideaki, Yamamoto, Hidefumi, Senba, Masachika, Oishi, Kazunori, Suzuki, Shoichi, Fukushima, Kenichi, ...

To elucidate the mechanism of the high incidence of lower respiratory tract infections in patients with diabetes mellitus, we investigated the kinetics of production of macrophage inflammatory...

Fourteen-Member Macrolides Suppress Interleukin-8 Production but Do Not Promote Apoptosis of Activated Neutrophils

Tsuchihashi, Yoshiko, Oishi, Kazunori, Yoshimine, Hiroyuki, Suzuki, Shoichi, Kumatori, Atsushi, Sunazuka, Toshiaki, ...

A 14-member macrolide was found to inhibit interleukin-8 (IL-8) synthesis in lipopolysaccharide-stimulated neutrophils but did not accelerate apoptosis in activated neutrophils. These data suggest...

Fourteen-Member Macrolides Promote the Phosphatidylserine Receptor-Dependent Phagocytosis of Apoptotic Neutrophils by Alveolar Macrophages

Yamaryo, Takeshi, Oishi, Kazunori, Yoshimine, Hiroyuki, Tsuchihashi, Yoshiko, Matsushima, Kouji, Nagatake, Tsuyoshi

An inflammation of the airway of patients with diffuse panbronchiolitis (DPB), is characterized by dense neutrophil infiltration. Resolution of the inflammation can be achieved by the removal of...

ADAMTS-1: a metalloproteinase-disintegrin essential for normal growth, fertility, and organ morphology and function

Shindo, Takayuki, Kurihara, Hiroki, Kuno, Kouji, Yokoyama, Hitoshi, Wada, Takashi, Kurihara, Yukiko, ...

A disintegrin and metalloproteinase (ADAM) represents a protein family possessing both metalloproteinase and disintegrin domains. ADAMTS-1, an ADAM family member cloned from cachexigenic colon...

Analysis of mRNA With Microsomal Fractionation Using a SAGE-Based DNA Microarray System Facilitates Identification of the Genes Encoding Secretory Proteins

Toyoda , Nobuaki, Nagai, Shigenori, Terashima, Yuya, Motomura, Kazushi, Haino, Makoto, Hashimoto, Shin-ichi, ...

In the regulation of host defense responses such as inflammation and immunity, the secretory proteins, including membrane proteins, play central roles. Although many secretory proteins have been...

A functional granulocyte colony-stimulating factor receptor is required for normal chemoattractant-induced neutrophil activation

Betsuyaku, Tomoko, Liu, Fulu, Senior, Robert M., Haug, Jeffery S., Brown, Eric J., Jones, Samuel L., ...

Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic growth factor that is widely used to treat neutropenia. In addition to stimulating polymorphonuclear neutrophil (PMN) production,...

Active participation of CCR5+CD8+ T lymphocytes in the pathogenesis of liver injury in graft-versus-host disease

Murai, Masako, Yoneyama, Hiroyuki, Harada, Akihisa, Yi, Zhang, Vestergaard, Christian, Guo, Baoyu, ...

We examined the molecular pathogenesis of graft-versus-host disease–associated (GVHD-associated) liver injury in mice, focusing on the role of chemokines. At the second week after cell transfer in...

Overproduction of Th2-specific chemokines in NC/Nga mice exhibiting atopic dermatitis–like lesions

Vestergaard, Christian, Yoneyama, Hiroyuki, Murai, Masako, Nakamura, Kohichiro, Tamaki, Kunihiko, Terashima, Yuya, ...

We have examined the expression of chemokines and their receptors in the atopic dermatitis–like (AD-like) lesions of NC/Nga mice. Such lesions develop when the mice are kept in conventional...

5′SAGE: 5′-end Serial Analysis of Gene Expression database

Kasai, Yasuhiro, Hashimoto, Shin-ichi, Yamada, Tomoyuki, Sese, Jun, Sugano, Sumio, Matsushima, Kouji, ...

To comprehensively identify transcription start sites and the frequencies of individual mRNAs in human cell libraries, a method of 5′ end Serial Analysis of Gene Expression (SAGE) was developed...

Chemokines in renal diseases

Wada, Takashi, Yukoyama, Hitoshi, Matsushima, Kouji, Kobayashi, Kenichi, 和田, 隆志

The interaction of activated leukocytes and renal resident cells is thought to play a crucial role in the pathogenesis of renal diseases. Recent investigations of the pathophysiological roles of...

Chemokines in renal diseases

Wada, Takashi, Yukoyama, Hitoshi, Matsushima, Kouji, Kobayashi, Ken-ichi

The interaction of activated leukocytes and renal resident cells is thought to play a crucial role in the pathogenesis of renal diseases. Recent investigations of the pathophysiological roles of...

Chemokines in renal diseases

Wada, Takashi, Yukoyama, Hitoshi, Matsushima, Kouji, Kobayashi, Kenichi, 和田, 隆志

The interaction of activated leukocytes and renal resident cells is thought to play a crucial role in the pathogenesis of renal diseases. Recent investigations of the pathophysiological roles of...

Regulation of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) and TRAIL receptor expression in human neutrophils

Kamohara, Hidenobu, Matsuyama, Wataru, Shimozato, Osamu, Abe, Koichiro, Galligan, Carole, Hashimoto, Shin-Ichi, ...

Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily, which is capable of inducing apoptosis in many cell types, including tumour and...

Secondary lymphoid tissue chemokine (SLC/CCL21)/CCR7 signaling regulates fibrocytes in renal fibrosis

Sakai, Norihiko, Wada, Takashi, Yokoyama, Hitoshi, Lipp, Martin, Ueha, Satoshi, Matsushima, Kouji, ...

Fibrocytes are a distinct population of bloodborne cells that share markers of leukocytes as well as mesenchymal cells. We hypothesized that CCR7-positive fibrocytes migrate into the kidney in...

Blockade of CCR2 Ameliorates Progressive Fibrosis in Kidney

Kitagawa, Kiyoki, Wada, Takashi, Furuichi, Kengo, Hashimoto, Hiroyuki, Ishiwata, Yoshiro, Asano, Masahide, ...

Fibrosis is a hallmark of progressive organ diseases. Monocyte chemoattractant protein (MCP)-1, also termed as macrophage chemotactic and activating factor (MCAF/CCL2) and its receptor, CCR2 are...

Indigenous Pulmonary Propionibacterium acnes Primes the Host in the Development of Sarcoid-Like Pulmonary Granulomatosis in Mice

Nishiwaki, Tetsu, Yoneyama, Hiroyuki, Eishi, Yoshinobu, Matsuo, Naoki, Tatsumi, Koichiro, Kimura, Hiroshi, ...

Although many cases of sarcoidosis are self-limiting with spontaneous remission, uncontrolled pulmonary granulomatosis with fibrosis produces intolerable long-term respiratory symptoms in a minority...

Macrophage-Derived Chemokine (MDC/CCL22) and CCR4 Are Involved in the Formation of T Lymphocyte-Dendritic Cell Clusters in Human Inflamed Skin and Secondary Lymphoid Tissue

Katou, Fuminori, Ohtani, Haruo, Nakayama, Takashi, Ono, Katsuhiko, Matsushima, Kouji, Saaristo, Anne, ...

Our previous study demonstrated formation of T cell-dendritic cell (DC) clusters in inflamed dermis of intraorally autotransplanted skin flaps. Such T cell-DC clusters are supposed to be important...

Essential Roles of the CC Chemokine Ligand 3-CC Chemokine Receptor 5 Axis in Bleomycin-Induced Pulmonary Fibrosis through Regulation of Macrophage and Fibrocyte Infiltration

Ishida, Yuko, Kimura, Akihiko, Kondo, Toshikazu, Hayashi, Takahito, Ueno, Masaya, Takakura, Nobuyuki, ...

We investigated the pathogenic roles of CC chemokine ligand (CCL)3 and its receptors, CC chemokine receptor (CCR)1 and CCR5, in bleomycin (BLM)-induced pulmonary fibrosis (PF). An intratracheal...

Effect of thromboxane A2 antagonists on bronchial hyperresponsiveness induced immediately after interleukin-8 inhalation in guinea-pigs

Fujimura, Masaki, Myou, Shigeharu, Nomura, Masaharu, Matsuda, Tamotsu, Harada, Akihisa, Mukaida, Naofumi, ...

Although repeated intranasal administration of interleukin-8 (IL-8) causes bronchial hyperresponsiveness (BHR) mediated via thromboxane A2 (TXA2) and airway neutrophil accumulation in guinea-pigs,...

Induction of histidine decarboxylase in skeletal muscle in mice by electrical stimulation, prolonged walking and interleukin-1

Endo, Yasuo, Tabata, Takayoshi, Kuroda, Hiroshi, Tadano, Takeshi, Matsushima, Kouji, Watanabe, Makoto

In normal non-exercised skeletal muscles in mice, the activity of histidine decarboxylase (HDC), the enzyme which forms histamine, was very low.HDC activity in the quadriceps femoris muscle was...

UTGB/medaka: genomic resource database for medaka biology

Ahsan, Budrul, Kobayashi, Daisuke, Yamada, Tomoyuki, Kasahara, Masahiro, Sasaki, Shin, Saito, Taro L., ...

Medaka (Oryzias latipes) is a small egg-laying freshwater teleost native to East Asia that has become an excellent model system for developmental genetics and evolutionary biology. The draft medaka...

Plasmacytoid DCs help lymph node DCs to induce anti-HSV CTLs

Yoneyama, Hiroyuki, Matsuno, Kenjiro, Toda, Etsuko, Nishiwaki, Tetsu, Matsuo, Naoki, Nakano, Akiko, ...

Antiviral cell–mediated immunity is initiated by the dendritic cell (DC) network in lymph nodes (LNs). Plasmacytoid DCs (pDCs) are known to migrate to inflamed LNs and produce interferon (IFN)-α,...

Aberrant High Expression of B Lymphocyte Chemokine (Blc/Cxcl13) by C11b+Cd11c+ Dendritic Cells in Murine Lupus and Preferential Chemotaxis of B1 Cells towards Blc

Ishikawa, Sho, Sato, Taku, Abe, Masaaki, Nagai, Shigenori, Onai, Nobuyuki, Yoneyama, Hiroyuki, ...

We observed here that the expression of B lymphocyte chemokine (BLC/CXCL13) was markedly enhanced in the thymus and kidney in aged (NZB × NZW)F1 (BWF1) mice developing lupus nephritis, but not in...

Pivotal Role of Dendritic Cell–derived CXCL10 in the Retention of T Helper Cell 1 Lymphocytes in Secondary Lymph Nodes

Yoneyama, Hiroyuki, Narumi, Shosaku, Zhang, Yanyun, Murai, Masako, Baggiolini, Marco, Lanzavecchia, Antonio, ...

Various immune diseases are considered to be regulated by the balance of T helper (Th)1 and Th2 subsets. Although Th lymphocytes are believed to be generated in draining lymph nodes (LNs), in vivo Th...

Regulation by Chemokines of Circulating Dendritic Cell Precursors, and the Formation of Portal Tract–Associated Lymphoid Tissue, in a Granulomatous Liver Disease

Yoneyama, Hiroyuki, Matsuno, Kenjiro, Zhang, Yanyun, Murai, Masako, Itakura, Meiji, Ishikawa, Sho, ...

We have studied the recruitment and roles of distinct dendritic cell (DC) precursors from the circulation into Propionibacterium acnes–induced granulomas in mouse liver. During infection,...

The α Chemokine, Interleukin 8, Inhibits the Antiviral Action of Interferon α

Khabar, Khalid S.A., Al-Zoghaibi, Fahad, Al-Ahdal, Mohammed N., Murayama, Tsugiya, Dhalla, Mohammed, Mukaida, Naofumi, ...

Interferon (IFN) exhibits a potent antiviral activity in vitro and plays a major role in the early defense against viruses. Like IFN, the proinflammatory chemokine, interleukin (IL)-8, is induced by...

High-Resolution Analysis of the 5′-End Transcriptome Using a Next Generation DNA Sequencer

Hashimoto, Shin-ichi, Qu, Wei, Ahsan, Budrul, Ogoshi, Katsumi, Sasaki, Atsushi, Nakatani, Yoichiro, ...

Massively parallel, tag-based sequencing systems, such as the SOLiD system, hold the promise of revolutionizing the study of whole genome gene expression due to the number of data points that can be...

Massive transcriptional start site analysis of human genes in hypoxia cells

Tsuchihara, Katsuya, Suzuki, Yutaka, Wakaguri, Hiroyuki, Irie, Takuma, Tanimoto, Kousuke, Hashimoto, Shin-ichi, ...

Combining our full-length cDNA method and the massively parallel sequencing technology, we developed a simple method to collect precise positional information of transcriptional start sites (TSSs)...

MachiBase: a Drosophila melanogaster 5′-end mRNA transcription database

Ahsan, Budrul, Saito, Taro L., Hashimoto, Shin-ichi, Muramatsu, Keigo, Tsuda, Manabu, Sasaki, Atsushi, ...

MachiBase (http://machibase.gi.k.u-tokyo.ac.jp/) provides a comprehensive and freely accessible resource regarding Drosophila melanogaster 5′-end mRNA transcription at different developmental...

Increased Foxp3+ CD4+ Regulatory T Cells with Intact Suppressive Activity but Altered Cellular Localization in Murine Lupus

Abe, Jun, Ueha, Satoshi, Suzuki, Jun, Tokano, Yoshiaki, Matsushima, Kouji, Ishikawa, Sho

Foxp3+ CD4+ regulatory T (Treg) cells play a pivotal role in the maintenance of dominant self tolerance. Understanding how the failures of immune control by Treg cells are involved in autoimmune...