Isolation and Identification of an Actin Gene From Babesia gibsoni (2006)
Jinlin Zhou, Bing Huang, Hiroshi Suzuki, Kozo Fujisaki, Ikuo Igarashi, Xuenan Xuan
Rika Umemiya, Minoru Fukuda, Kozo Fujisaki, Toshihiro Matsui
Cryptosporidium parvum mainly invades the intestinal epithelium and causes watery diarrhea in humans and calves. However, the invasion process has not yet been clarified. In the present study, the...
Irungu Gaturaga, Bayin Chahan, Xuenan Xuan, Xiaohong Huang, Min Liao, Shinya Fukumoto, ...
The surface antigen 1–related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal...
Tomohide Matsuo, Noboru Inoue, Martin R. Ruheta, DeMar Taylor, Kozo Fujisaki
In the present study, monoclonal antibodies (mAbs) against adult Ornithodoros moubata hemocytes were established. Afterward, artificial feeding was performed to assess the tickcidal effect of fetal...
Shinya Fukumoto, Yukiko Sekine, Xuenan Xuan, Ikuo Igarashi, Chihiro Sugimoto, Hideyuki Nagasawa, ...
The entire P50 gene encoding a surface protein of Babesia gibsoni was cloned into the bacteria expression vector pGEX-4T-3 and subsequently expressed in Escherichia coli as a glutathione...
Sabine Bork, Naoaki Yokoyama, Tomohide Matsuo, Florencia G. Claveria, Kozo Fujisaki, Ikuo Igarashi
The antifungal agents clotrimazole (CLT) and ketoconazole (KC) and the herbicide clodinafop-propargyl (CP) inhibit growth of Plasmodium sp., Toxoplasma sp., and Trypanosoma sp. In the present study,...
Badgar Battsetseg, Kamilius Mamiro, Noboru Inoue, Levi Makala, Hideyuki Nagasawa, Yoichiro Iwakura, ...
To investigate the immunological mechanisms of acquired resistance to tick infestation, interferon gamma (IFN-γ) deficient mice (IFN-γ −/− mice) were used to assess interleukin-4 (IL-4) and...
Characterization of Phagocytic Hemocytes in Ornithodoros moubata (Acari: Ixodidae) (2001)
Noboru Inoue, Kayoko Hanada, Naotoshi Tsuji, Ikuo Igarashi, Hideyuki Nagasawa, Takeshi Mikami, ...
Effects of fetal bovine serum (FBS) and complement on phagocytic activity in Ornithodoros moubata (Murray 1877) hemocytes and protease activity in the hemocytes were examined. At least three...
Ikadai, Hiromi, Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Shigeyasu, Kanemaru, Takumi, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of B. caballi merozoite. A cDNA encoding a 48-kDa...
Xuan, Xuenan, Larsen, Alejandra, Ikadai, Hiromi, Tanaka, Tetsuya, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
The gene encoding the entire Babesia equi merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Nishikawa, Yoshifumi, Kousaka, Yuko, Tragoolpua, Khajornsak, Xuan, Xuenan, Makala, Levi, Fujisaki, Kozo, ...
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...
Hirata, Haruyuki, Ikadai, Hiromi, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
To isolate Babesia equi genes encoding immunodominant proteins, a cDNA expression library prepared from B. equi mRNA was immunoscreened with B. equi-infected horse serum. Eighteen positive cDNA...
Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, Nishikawa, Yoshifumi, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported...
Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...
The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...
Nagai, Akiko, Yokoyama, Naoaki, Matsuo, Tomohide, Bork, Sabine, Hirata, Haruyuki, Xuan, Xuenan, ...
Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Tamaki, Yoh, Hirata, Haruyuki, Takabatake, Noriyuki, Bork, Sabine, Yokoyama, Naoaki, Xuan, Xuenan, ...
A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Hirata, Haruyuki, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, Igarashi, Ikuo
In this study, we characterized a Babesia equi Be158 gene obtained by immunoscreening a B. equi cDNA expression phage library with B. equi-infected horse serum. The Be158 gene consists of an open...
Liao, Min, Zhang, Shoufa, Xuan, Xuenan, Zhang, Guohong, Huang, Xiaohong, Igarashi, Ikuo, ...
An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to...
Ikadai, Hiromi, Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Shigeyasu, Kanemaru, Takumi, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia caballi merozoite mRNA was screened with a monoclonal antibody BC11D against the rhoptry protein of B. caballi merozoite. A cDNA encoding a 48-kDa...
Xuan, Xuenan, Larsen, Alejandra, Ikadai, Hiromi, Tanaka, Tetsuya, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
The gene encoding the entire Babesia equi merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was...
Fukumoto, Shinya, Xuan, Xuenan, Nishikawa, Yoshifumi, Inoue, Noboru, Igarashi, Ikuo, Nagasawa, Hideyuki, ...
A cDNA expression library prepared from Babesia gibsoni merozoite mRNA was screened with B. gibsoni-infected dog serum. cDNA encoding a 50-kDa protein was cloned and designated the P50 gene. The...
Nishikawa, Yoshifumi, Kousaka, Yuko, Tragoolpua, Khajornsak, Xuan, Xuenan, Makala, Levi, Fujisaki, Kozo, ...
The baculovirus expression system has proved to be a useful tool for the production of recombinant proteins. Here we have characterized the Neospora caninum surface protein NcSRS2 produced by two...
Xuan, Xuenan, Igarashi, Ikuo, Tanaka, Tetsuya, Fukumoto, Shinya, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A latex agglutination test (LAT) using recombinant equi merozoite antigen 1 (EMA-1) for the detection of antibodies to Babesia equi was developed. The LAT was able to differentiate very clearly...
Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...
A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...
Hirata, Haruyuki, Ikadai, Hiromi, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
To isolate Babesia equi genes encoding immunodominant proteins, a cDNA expression library prepared from B. equi mRNA was immunoscreened with B. equi-infected horse serum. Eighteen positive cDNA...
Hirata, Haruyuki, Xuan, Xuenan, Yokoyama, Naoaki, Nishikawa, Yoshifumi, Fujisaki, Kozo, Suzuki, Naoyoshi, ...
The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported...
Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...
The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...
Nagai, Akiko, Yokoyama, Naoaki, Matsuo, Tomohide, Bork, Sabine, Hirata, Haruyuki, Xuan, Xuenan, ...
Three antimalarial drugs, artesunate, pyrimethamine, and pamaquine, were evaluated for their growth-inhibitory effects against Babesia equi and Babesia caballi in in vitro culture. B. equi was more...
Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...
Tamaki, Yoh, Hirata, Haruyuki, Takabatake, Noriyuki, Bork, Sabine, Yokoyama, Naoaki, Xuan, Xuenan, ...
A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could...
Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...
The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....
Hirata, Haruyuki, Yokoyama, Naoaki, Xuan, Xuenan, Fujisaki, Kozo, Suzuki, Naoyoshi, Igarashi, Ikuo
In this study, we characterized a Babesia equi Be158 gene obtained by immunoscreening a B. equi cDNA expression phage library with B. equi-infected horse serum. The Be158 gene consists of an open...
Liao, Min, Zhang, Shoufa, Xuan, Xuenan, Zhang, Guohong, Huang, Xiaohong, Igarashi, Ikuo, ...
An immunochromatographic test (ICT) with recombinant surface antigen 1 of Neospora caninum (NcSAG1) was developed for the rapid detection of antibodies to N. caninum in cattle. The ICT was used to...
Babesial Vector Tick Defensin against Babesia sp. Parasites▿ †
Tsuji, Naotoshi, Battsetseg, Badgar, Boldbaatar, Damdinsuren, Miyoshi, Takeharu, Xuan, Xuenan, Oliver, James H., ...
Antimicrobial peptides are major components of host innate immunity, a well-conserved, evolutionarily ancient defensive mechanism. Infectious disease-bearing vector ticks are thought to possess...
A Cysteine Protease Is Critical for Babesia spp. Transmission in Haemaphysalis Ticks
Tsuji, Naotoshi, Miyoshi, Takeharu, Battsetseg, Badger, Matsuo, Tomohide, Xuan, Xuenan, Fujisaki, Kozo
Vector ticks possess a unique system that enables them to digest large amounts of host blood and to transmit various animal and human pathogens, suggesting the existence of evolutionally acquired...