A. Lacey Samuels, Thomas H. Giddings, L. Andrew Staehelin
Abstract. Cell plate formation in tobacco root tips and synchronized dividing suspension cultured tobacco BY-2 cells was examined using cryofixation and immu-nocytochemical methods. Due to the much...
Erin A. White, L. Andrew Staehelin
We have investigated the process of somatic-type cytokinesis in Arabidopsis (Arabidopsis thaliana) meristem cells with a three-dimensional resolution of ~7 nm by electron tomography of high-pressure...
Christopher, David A, Borsics, Tamas, Yuen, Christen YL, Ullmer, Wendy, Andème-Ondzighi, Christine, Andres, Marilou A, ...
Abstract Background The cyclic nucleotide-gated ion channels (CNGCs) maintain cation homeostasis essential for a wide range of physiological processes in plant cells. However, the precise subcellular...
Murata, Takashi, Karahara, Ichirou, Kozuka, Toshiaki, Giddings, Thomas H., Staehelin, L. Andrew, Mineyuki, Yoshinobu
We have optimized the conditions for visualizing microfilaments, microtubules, and coated pits in the cortical cytoplasm of high‐pressure frozen and freeze‐substituted plant cells. In...
Golgi structure in three dimensions: functional insights from the normal rat kidney cell (1999)
Mark S. Ladinsky, David N. Mastronarde, J. Richard Mcintosh, Kathryn E. Howell, L. Andrew Staehelin
Abstract. Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at...
Nebenführ, Andreas, Frohlick, Jennifer A., Staehelin, L. Andrew
We have followed the redistribution of Golgi stacks during mitosis and cytokinesis in living tobacco BY-2 suspension culture cells by means of a green fluorescent protein-tagged soybean α-1,2...
Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System1
Nebenführ, Andreas, Gallagher, Larry A., Dunahay, Terri G., Frohlick, Jennifer A., Mazurkiewicz, Anna M., Meehl, Janet B., ...
The Golgi apparatus in plant cells consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that appear to be randomly distributed throughout the cytoplasm. To...
Zheng, Hui Qiong, Staehelin, L. Andrew
The endoplasmic reticulum (ER) of columella root cap cells has been postulated to play a role in gravity sensing. We have re-examined the ultrastructure of columella cells in tobacco (Nicotiana...
Yoder, Thomas L., Zheng, Hui-qiong, Todd, Paul, Staehelin, L. Andrew
Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic...
Otegui, Marisa S., Mastronarde, David N., Kang, Byung-Ho, Bednarek, Sebastian Y., Staehelin, L. Andrew
The three-dimensional architecture of syncytial-type cell plates in the endosperm of Arabidopsis has been analyzed at ∼6-nm resolution by means of dual-axis high-voltage electron tomography of...
Otegui, Marisa, Staehelin, L. Andrew
Cell wall formation in the syncytial endosperm of Arabidopsis was studied by using high-pressure-frozen/freeze-substituted developing seeds and immunocytochemical techniques. The endosperm...
Ritzenthaler, Christophe, Nebenführ, Andreas, Movafeghi, Ali, Stussi-Garaud, Christiane, Behnia, Leila, Pimpl, Peter, ...
Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus. Although this toxin has been used in many studies, its effects on...
Otegui, Marisa S., Capp, Roberta, Staehelin, L. Andrew
Mineral-accumulating compartments in developing seeds of Arabidopsis were studied using high-pressure-frozen/freeze-substituted samples. Developing seeds store minerals in three locations: in the...
Tomographic Evidence for Continuous Turnover of Golgi Cisternae in Pichia pastorisV⃞
Mogelsvang, Soren, Gomez-Ospina, Natalia, Soderholm, Jon, Glick, Benjamin S., Staehelin, L. Andrew
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure–function studies of the...
Giddings, Thomas H., Withers, Nancy W., Staehelin, L. Andrew
Freeze-fracture replicas of the photosynthetic prokaryote Prochloron sp., collected at Coconut Island, Hawaii, show that the thylakoids are differentiated into stacked and unstacked regions much like...
Seguí-Simarro, José M., Austin, Jotham R., White, Erin A., Staehelin, L. Andrew
We have investigated the process of somatic-type cytokinesis in Arabidopsis (Arabidopsis thaliana) meristem cells with a three-dimensional resolution of ∼7 nm by electron tomography of...
Gap junctions are specialized membrane regions that seem to mediate intercellular communication. They appear to contain closely packed arrays of equally sized particles all of which, upon...
Fernandez, Donna E., Staehelin, L. Andrew
We have examined the effect of gibberellic acid (GA3) on the distribution of the enzyme responsible for mobilizing storage triacylglycerol in aleurone cells of Hordeum vulgare L. cv Himalaya. Using...
Fernandez, Donna E., Qu, Rongda, Huang, Anthony H. C., Staehelin, L. Andrew
We have used antibodies directed against the 16.5 kilodalton protein L3, the most abundant integral protein of maize (Zea mays L. cv Mo 17) lipid bodies, to follow the fate of this protein in...
Greene, Ben A., Staehelin, L. Andrew, Melis, Anastasios
Characterization of the functional organization of the photochemical apparatus in the light sensitive chlorophyll b-deficient oil yellow-yellow green (OY-YG) mutant of maize (Zea mays) is presented....
Moore, Patricia J., Darvill, Alan G., Albersheim, Peter, Staehelin, L. Andrew
Plant cell walls serve several functions: they impart rigidity to the plant, provide a physical and chemical barrier between the cell and its environment, and regulate the size and shape of each...
A Second Extensin-Like Hydroxyproline-Rich Glycoprotein from Carrot Cell Walls 1
Stafstrom, Joel P., Staehelin, L. Andrew
The insoluble extensin matrix of dicot cell walls has been studied most fruitfully by examining the salt-extractable precursors to this matrix. Multiple extensin-like hydroxyproline-rich...
Allred, David R., Staehelin, L. Andrew
We have visualized directly the distribution of the cytochrome b6/f and coupling factor ATP synthetase complexes in thylakoid membranes of embedded, thin-sectioned, intact chloroplasts by using...
Dunahay, Terri G., Staehelin, L. Andrew
We have used the nonionic detergent octyl-β-d-glucopyranoside in combination with sodium dodecyl sulfate to isolate two novel Photosystem I (PSI) complexes from spinach (Spinacea oleracea L.)...
Structure of the Thylakoids and Envelope Membranes of the Cyanelles of Cyanophora paradoxa1
Giddings, Thomas H., Wasmann, Cathy, Staehelin, L. Andrew
The cyanelles of Cyanophora paradoxa Korsch. are photosynthetically active obligate endosymbionts in which phycobiliproteins serve as the major accessory pigments. Freeze-fracture electron...
Sprague, Sallie G., Staehelin, L. Andrew
Comparison of several lipid reconstitution methods showed that they were not equally efficient at transferring the predominant thylakoid lipid, monogalactosyldiglyceride (MG), to the aqueous phase....
Isolation and Characterization of a New Minor Chlorophyll a/b-Protein Complex (CP24) from Spinach 1
Dunahay, Terri G., Staehelin, L. Andrew
We have identified a new minor chlorophyll a/b-protein complex in the thylakoid membranes of spinach (Spinacia oleracea L.), which migrates as a green band below CPII on mildly denaturing...
Cross-Linking Patterns in Salt-Extractable Extensin from Carrot Cell Walls 1
Stafstrom, Joel P., Staehelin, L. Andrew
Extensins are hydroxyproline-rich glycoproteins (HRGPs) found in the primary cell walls of dicots. Extensin monomers are secreted into the wall and covalently bound to each other, presumably by...
The Role of Carbohydrate in Maintaining Extensin in an Extended Conformation 1
Stafstrom, Joel P., Staehelin, L. Andrew
Monomers of the plant cell wall glycoprotein extensin are secreted into the wall where they become cross-linked to each other to form a rigid matrix. Expression of the extensin matrix is correlated...
Allen, Keith D., Staehelin, L. Andrew
The photosystem (PS) II antenna system comprises several biochemically and spectroscopically distinct complexes, including light-harvesting complex II (LHCII), chlorophyll-protein complex (CP) 29,...
Zhang, Guo Feng, Staehelin, L. Andrew
The Golgi apparatus of plant cells is engaged in both the processing of glycoproteins and the synthesis of complex polysaccharides. To investigate the compartmentalization of these functions within...
Nebenführ, Andreas, Frohlick, Jennifer A., Staehelin, L. Andrew
We have followed the redistribution of Golgi stacks during mitosis and cytokinesis in living tobacco BY-2 suspension culture cells by means of a green fluorescent protein-tagged soybean α-1,2...
Stop-and-Go Movements of Plant Golgi Stacks Are Mediated by the Acto-Myosin System1
Nebenführ, Andreas, Gallagher, Larry A., Dunahay, Terri G., Frohlick, Jennifer A., Mazurkiewicz, Anna M., Meehl, Janet B., ...
The Golgi apparatus in plant cells consists of a large number of independent Golgi stack/trans-Golgi network/Golgi matrix units that appear to be randomly distributed throughout the cytoplasm. To...
Zheng, Hui Qiong, Staehelin, L. Andrew
The endoplasmic reticulum (ER) of columella root cap cells has been postulated to play a role in gravity sensing. We have re-examined the ultrastructure of columella cells in tobacco (Nicotiana...
Yoder, Thomas L., Zheng, Hui-qiong, Todd, Paul, Staehelin, L. Andrew
Quantitative analysis of statolith sedimentation behavior was accomplished using videomicroscopy of living columella cells of corn (Zea mays) roots, which displayed no systematic cytoplasmic...
Otegui, Marisa S., Mastronarde, David N., Kang, Byung-Ho, Bednarek, Sebastian Y., Staehelin, L. Andrew
The three-dimensional architecture of syncytial-type cell plates in the endosperm of Arabidopsis has been analyzed at ∼6-nm resolution by means of dual-axis high-voltage electron tomography of...
Otegui, Marisa, Staehelin, L. Andrew
Cell wall formation in the syncytial endosperm of Arabidopsis was studied by using high-pressure-frozen/freeze-substituted developing seeds and immunocytochemical techniques. The endosperm...
Ritzenthaler, Christophe, Nebenführ, Andreas, Movafeghi, Ali, Stussi-Garaud, Christiane, Behnia, Leila, Pimpl, Peter, ...
Brefeldin A (BFA) causes a block in the secretory system of eukaryotic cells by inhibiting vesicle formation at the Golgi apparatus. Although this toxin has been used in many studies, its effects on...
Otegui, Marisa S., Capp, Roberta, Staehelin, L. Andrew
Mineral-accumulating compartments in developing seeds of Arabidopsis were studied using high-pressure-frozen/freeze-substituted samples. Developing seeds store minerals in three locations: in the...
Tomographic Evidence for Continuous Turnover of Golgi Cisternae in Pichia pastorisV⃞
Mogelsvang, Soren, Gomez-Ospina, Natalia, Soderholm, Jon, Glick, Benjamin S., Staehelin, L. Andrew
The budding yeast Pichia pastoris contains ordered Golgi stacks next to discrete transitional endoplasmic reticulum (tER) sites, making this organism ideal for structure–function studies of the...
Giddings, Thomas H., Withers, Nancy W., Staehelin, L. Andrew
Freeze-fracture replicas of the photosynthetic prokaryote Prochloron sp., collected at Coconut Island, Hawaii, show that the thylakoids are differentiated into stacked and unstacked regions much like...
Seguí-Simarro, José M., Austin, Jotham R., White, Erin A., Staehelin, L. Andrew
We have investigated the process of somatic-type cytokinesis in Arabidopsis (Arabidopsis thaliana) meristem cells with a three-dimensional resolution of ∼7 nm by electron tomography of...
Gap junctions are specialized membrane regions that seem to mediate intercellular communication. They appear to contain closely packed arrays of equally sized particles all of which, upon...
Fernandez, Donna E., Staehelin, L. Andrew
We have examined the effect of gibberellic acid (GA3) on the distribution of the enzyme responsible for mobilizing storage triacylglycerol in aleurone cells of Hordeum vulgare L. cv Himalaya. Using...
Fernandez, Donna E., Qu, Rongda, Huang, Anthony H. C., Staehelin, L. Andrew
We have used antibodies directed against the 16.5 kilodalton protein L3, the most abundant integral protein of maize (Zea mays L. cv Mo 17) lipid bodies, to follow the fate of this protein in...
Greene, Ben A., Staehelin, L. Andrew, Melis, Anastasios
Characterization of the functional organization of the photochemical apparatus in the light sensitive chlorophyll b-deficient oil yellow-yellow green (OY-YG) mutant of maize (Zea mays) is presented....
Moore, Patricia J., Darvill, Alan G., Albersheim, Peter, Staehelin, L. Andrew
Plant cell walls serve several functions: they impart rigidity to the plant, provide a physical and chemical barrier between the cell and its environment, and regulate the size and shape of each...
A Second Extensin-Like Hydroxyproline-Rich Glycoprotein from Carrot Cell Walls 1
Stafstrom, Joel P., Staehelin, L. Andrew
The insoluble extensin matrix of dicot cell walls has been studied most fruitfully by examining the salt-extractable precursors to this matrix. Multiple extensin-like hydroxyproline-rich...
Allred, David R., Staehelin, L. Andrew
We have visualized directly the distribution of the cytochrome b6/f and coupling factor ATP synthetase complexes in thylakoid membranes of embedded, thin-sectioned, intact chloroplasts by using...
Dunahay, Terri G., Staehelin, L. Andrew
We have used the nonionic detergent octyl-β-d-glucopyranoside in combination with sodium dodecyl sulfate to isolate two novel Photosystem I (PSI) complexes from spinach (Spinacea oleracea L.)...
Structure of the Thylakoids and Envelope Membranes of the Cyanelles of Cyanophora paradoxa1
Giddings, Thomas H., Wasmann, Cathy, Staehelin, L. Andrew
The cyanelles of Cyanophora paradoxa Korsch. are photosynthetically active obligate endosymbionts in which phycobiliproteins serve as the major accessory pigments. Freeze-fracture electron...
Sprague, Sallie G., Staehelin, L. Andrew
Comparison of several lipid reconstitution methods showed that they were not equally efficient at transferring the predominant thylakoid lipid, monogalactosyldiglyceride (MG), to the aqueous phase....
Isolation and Characterization of a New Minor Chlorophyll a/b-Protein Complex (CP24) from Spinach 1
Dunahay, Terri G., Staehelin, L. Andrew
We have identified a new minor chlorophyll a/b-protein complex in the thylakoid membranes of spinach (Spinacia oleracea L.), which migrates as a green band below CPII on mildly denaturing...
Cross-Linking Patterns in Salt-Extractable Extensin from Carrot Cell Walls 1
Stafstrom, Joel P., Staehelin, L. Andrew
Extensins are hydroxyproline-rich glycoproteins (HRGPs) found in the primary cell walls of dicots. Extensin monomers are secreted into the wall and covalently bound to each other, presumably by...
The Role of Carbohydrate in Maintaining Extensin in an Extended Conformation 1
Stafstrom, Joel P., Staehelin, L. Andrew
Monomers of the plant cell wall glycoprotein extensin are secreted into the wall where they become cross-linked to each other to form a rigid matrix. Expression of the extensin matrix is correlated...
Allen, Keith D., Staehelin, L. Andrew
The photosystem (PS) II antenna system comprises several biochemically and spectroscopically distinct complexes, including light-harvesting complex II (LHCII), chlorophyll-protein complex (CP) 29,...
Zhang, Guo Feng, Staehelin, L. Andrew
The Golgi apparatus of plant cells is engaged in both the processing of glycoproteins and the synthesis of complex polysaccharides. To investigate the compartmentalization of these functions within...
Austin, Jotham R., Frost, Elizabeth, Vidi, Pierre-Alexandre, Kessler, Felix, Staehelin, L. Andrew
Plastoglobules are lipoprotein particles inside chloroplasts. Their numbers have been shown to increase during the upregulation of plastid lipid metabolism in response to oxidative stress and during...
Donohoe, Bryon S., Kang, Byung-Ho, Staehelin, L. Andrew
Coat protein I (COPI) vesicles arise from Golgi cisternae and mediate the recycling of proteins from the Golgi back to the endoplasmic reticulum (ER) and the transport of Golgi resident proteins...
Otegui, Marisa S., Herder, Rachel, Schulze, Jan, Jung, Rudolf, Staehelin, L. Andrew
We have investigated the transport of storage proteins, their processing proteases, and the Vacuolar Sorting Receptor-1/Epidermal Growth Factor Receptor–Like Protein1 (VSR-1/ATELP1) receptor during...
Christopher, David A, Borsics, Tamas, Yuen, Christen YL, Ullmer, Wendy, Andème-Ondzighi, Christine, Andres, Marilou A, ...
Goodenough, Ursula W., Staehelin, L. Andrew
Wild-type chloroplast membranes from Chlamydomonas reinhardi exhibit four faces in freeze-etchreplicas: the complementary Bs and Cs faces are found where the membranes are stacked together; the...
Staehelin, L. Andrew, Chlapowski, Francis J., Bonneville, Mary A.
To determine the three-dimensional structure of the lumenal membrane of transitional epithelium, a study was made of sectioned, negatively stained, and freeze-etched specimens from intact epithelium...
LUMENAL PLASMA MEMBRANE OF THE URINARY BLADDER : II. Isolation and Structure of Membrane Components
Chlapowski, Francis J., Bonneville, Mary A., Staehelin, L. Andrew
A technique has been devised for isolation of lumenal plasma membranes from transitional epithelial cells lining the urinary bladder in rabbits and for subsequent separation of particle-bearing...
EFFECTS OF PHOSPHOTUNGSTATE NEGATIVE STAINING ON THE MORPHOLOGY OF THE ISOLATED GOLGI APPARATUS
Cunningham, William P., Staehelin, L. Andrew, Rubin, Robert W., Wilkins, Ross, Bonneville, Mary
Isolated Golgi complexes can be recognized in phosphotungstate (PTA) negative stain as stacks of membranous plates surrounded by a complex anastomosing network of tubules and vesicles. The extent of...
CILIARY MEMBRANE DIFFERENTIATIONS IN TETRAHYMENA PYRIFORMIS : Tetrahymena Has Four Types of Cilia
Sattler, Carol A., Staehelin, L. Andrew
We have examined thin sections and replicas of freeze-fractured cilia of Tetrahymena pyriformis. The ciliary necklace located at the base of all freeze-fractured oral and somatic cilia has been...
Golgi Structure in Three Dimensions: Functional Insights from the Normal Rat Kidney Cell
Ladinsky, Mark S., Mastronarde, David N., McIntosh, J. Richard, Howell, Kathryn E., Staehelin, L. Andrew
Three-dimensional reconstructions of portions of the Golgi complex from cryofixed, freeze-substituted normal rat kidney cells have been made by dual-axis, high-voltage EM tomography at ∼7-nm...
Seguí-Simarro, José M., Coronado, María José, Staehelin, L. Andrew
Plant cells exhibit a high rate of mitochondrial DNA (mtDNA) recombination. This implies that before cytokinesis, the different mitochondrial compartments must fuse to allow for mtDNA intermixing....
Leitz, Guenther, Kang, Byung-Ho, Schoenwaelder, Monica E.A., Staehelin, L. Andrew
The starch statolith hypothesis of gravity sensing in plants postulates that the sedimentation of statoliths in specialized statocytes (columella cells) provides the means for converting the...
Andème Ondzighi, Christine, Christopher, David A., Cho, Eun Ju, Chang, Shu-Choeng, Staehelin, L. Andrew
Protein disulfide isomerase (PDI) oxidizes, reduces, and isomerizes disulfide bonds, modulates redox responses, and chaperones proteins. The Arabidopsis thaliana genome contains 12 PDI genes, but...
Seguí-Simarro, José M, Staehelin, L Andrew
Plant mitochondria are typically portrayed as being small, oval organelles. However, a recent study has demonstrated that the chondriome of shoot apical meristem (SAM) cells of Arabidopsis thaliana...