Schizosaccharomyces pombe mutants that are defective in glycoprotein galactosylation.
Several mutants of Schizosaccharomyces pombe were obtained that are defective in protein glycosylation. One of the mutants, strain Sp550, makes galactomannoproteins with about half of the wild-type...
Schizosaccharomyces pombe glycosylation mutant with altered cell surface properties.
Ballou, C E, Ballou, L, Ball, G
Mutagenesis of Schizosaccharomyces pombe cells yielded a strain that made reduced amounts of invertase. A comparison of the O- and N-linked carbohydrate chains of the wild-type and mutant...
Häusler, A, Ballou, L, Ballou, C E, Robbins, P W
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity...
Vanadate-resistant yeast mutants are defective in protein glycosylation.
Ballou, L, Hitzeman, R A, Lewis, M S, Ballou, C E
Spontaneous recessive orthovanadate-resistant mutants of Saccharomyces cerevisiae were obtained in five complementation groups, and all show defects in protein glycosylation that mimic the previously...
Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
Ballou, L, Hernandez, L M, Alvarado, E, Ballou, C E
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae...
Ballou, L, Gopal, P, Krummel, B, Tammi, M, Ballou, C E
A mutant of Saccharomyces cerevisiae with the genotype mnn1 mnn2 mnn9 gls1 synthesizes mannoproteins with oligosaccharides having the composition Glc3Man10Glc-NAc2- owing to the mnn9 defect, which...
Temperature-sensitive glucosamine auxotroph of Saccharomyces cerevisiae.
Ballou, L, Grove, J R, Roon, R J, Wiggs, J, Ballou, C E
Temperature-sensitive revertants were isolated from Saccharomyces cerevisiae D-glucosamine auxotrophs previously obtained in this laboratory (W. L. Whelan and C. E. Ballou, J. Bacteriol....
Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
Tsai, P K, Ballou, L, Esmon, B, Schekman, R, Ballou, C E
The total cell wall mannoprotein has been isolated from a mutant of Saccharomyces cerevisiae that fails to remove the glucose units of the dolichol-linked precursor after transfer of the...
Schizosaccharomyces pombe mutants that are defective in glycoprotein galactosylation.
Several mutants of Schizosaccharomyces pombe were obtained that are defective in protein glycosylation. One of the mutants, strain Sp550, makes galactomannoproteins with about half of the wild-type...
Schizosaccharomyces pombe glycosylation mutant with altered cell surface properties.
Ballou, C E, Ballou, L, Ball, G
Mutagenesis of Schizosaccharomyces pombe cells yielded a strain that made reduced amounts of invertase. A comparison of the O- and N-linked carbohydrate chains of the wild-type and mutant...
Häusler, A, Ballou, L, Ballou, C E, Robbins, P W
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity...
Vanadate-resistant yeast mutants are defective in protein glycosylation.
Ballou, L, Hitzeman, R A, Lewis, M S, Ballou, C E
Spontaneous recessive orthovanadate-resistant mutants of Saccharomyces cerevisiae were obtained in five complementation groups, and all show defects in protein glycosylation that mimic the previously...
Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
Ballou, L, Hernandez, L M, Alvarado, E, Ballou, C E
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae...
Ballou, L, Gopal, P, Krummel, B, Tammi, M, Ballou, C E
A mutant of Saccharomyces cerevisiae with the genotype mnn1 mnn2 mnn9 gls1 synthesizes mannoproteins with oligosaccharides having the composition Glc3Man10Glc-NAc2- owing to the mnn9 defect, which...
Temperature-sensitive glucosamine auxotroph of Saccharomyces cerevisiae.
Ballou, L, Grove, J R, Roon, R J, Wiggs, J, Ballou, C E
Temperature-sensitive revertants were isolated from Saccharomyces cerevisiae D-glucosamine auxotrophs previously obtained in this laboratory (W. L. Whelan and C. E. Ballou, J. Bacteriol....
Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
Tsai, P K, Ballou, L, Esmon, B, Schekman, R, Ballou, C E
The total cell wall mannoprotein has been isolated from a mutant of Saccharomyces cerevisiae that fails to remove the glucose units of the dolichol-linked precursor after transfer of the...