BIOSYNTHESIS OF THE PHENYLPROPANOID MOIETY OF CHLORAMPHENICOL, (1998)
Vining,L. C., Westlake,D. W. S.
Cultures of Streptomyces sp. 3022a were grown in the presence of C14-labelled substrates and incorporation of radioactivity into chloramphenicol measured. D-Glucose, labelled in carbons 1 or 2 or...
A method has been developed for the quantitative analysis of sugars and polyols accumulated in the mycelium of fungi. The constituents of the neutral, water-soluble material extracted from the cells...
ISOPHENOXAZINE SYNTHASE APOENZYME FROM PYCNOPORUS COCCINEUS, (1998)
A crystalline preparation of the apoenzyme of isophenoxazine synthase (2-aminophenol:O2 oxidoreductase) was obtained from Pycnoporus coccincus. The holoenzyme was reconstituted by addition of...
CINNAMIC ACID HYDROXYLASE IN SPINACH, (1998)
An acetone precipitate from an extract of spinach leaves catalysed the hydroxylation of trans-cinnamic acid to p-coumaric acid. The enzyme was unstable and could not be purified. Crude preparations...
PHENYLALANINE HYDROXYLASE FROM SPINACH LEAVES, (1998)
Spinach leaves contain an enzyme system which catalyses the hydroxylation of L-beta-phenylalanine to tyrosine. The crude active extract has been partially purified by fractional precipitation with...
McInnes,A. G., Smith,D. G., Vining,L. C., Wright,J. L. C.
When carbon-13 is used as the isotopic tracer in biosynthetic studies, the labelled atoms in the metabolite can often be determined by n.m.r. spectrometry, without need for chemical degradation. By...
Cyclonerodiol, a Sesquiterpene, Metabolite of Gibberella Fujikuroi, (1998)
Pitel,D. W., Arsenault,G. P., Vining,L. C.
A new sesquiterpene, cyclonerodiol with properties closely resembling those of the Gibberella metabolite was isolated from a species of Trichothesium. Direct comparison of samples (infrared, proton...
Production of Bikaverin by 'Fusarium oxysporum' and Its Identity with Lycopersin, (1998)
Brewer,D., Arsenault,G. P., Wright,J. L. C., Vining,L. C.
The presence of a wine-red antiprotozoal metabolite in cultures of G. fujikuroi (F. moniliforme) and another Fusarium species was first described in 1970 by Balan and coworkers. Subsequently, Kjaer,...
The nucleotide sequence of a region upstream of the type II polyketide synthase genes in the cluster for biosynthesis of the polyketide antibiotic jadomycin B in Streptomyces venezuelae contained an...
Madduri, K, Stuttard, C, Vining, L C
In actinomycetes that produce beta-lactam antibiotics of the cephem type, lysine epsilon-aminotransferase is the initial enzyme in the conversion of lysine to alpha-aminoadipic acid. We used a...
Tobin, M B, Kovacevic, S, Madduri, K, Hoskins, J A, Skatrud, P L, Vining, L C, ...
Lysine epsilon-aminotransferase (LAT) in the beta-lactam-producing actinomycetes is considered to be the first step in the antibiotic biosynthetic pathway. Cloning of restriction fragments from...
Madduri, K, Stuttard, C, Vining, L C
Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces...
Transductional analysis of chloramphenicol biosynthesis genes in Streptomyces venezuelae.
Vats, S, Stuttard, C, Vining, L C
Auxotrophs isolated from two chloramphenicol-nonproducing mutants of Streptomyces venezuelae included three requiring pyridoxal (Pxl-), VS248 (cml-11 pdx-2), VS253 (cml-11 pdx-3), and VS258 (cml-12...
Serine utilization by Klebsiella aerogenes.
Klebsiella aerogenes was found to contain a specific L-serine dehydrase that was induced by threonine, glycine or leucine, but not by its substrate. Cellular concentrations were sensitive to carbon...
Of seven chloramphenicol-producing actinomycetes examined, only Streptomyces venezuelae strain 13s contained extrachromosomal DNA detectable by agarose gel electrophoresis and cesium...
Francis, M M, Vining, L C, Westlake, D W
In Streptomyces sp. 3022a, anthranilate synthetase is composed of two non-identical subunits. The major subunit (molecular weight, 72,000) converts chorismic acid to anthranilic acid, using ammonia...
The nucleotide sequence of a region upstream of the type II polyketide synthase genes in the cluster for biosynthesis of the polyketide antibiotic jadomycin B in Streptomyces venezuelae contained an...
Madduri, K, Stuttard, C, Vining, L C
In actinomycetes that produce beta-lactam antibiotics of the cephem type, lysine epsilon-aminotransferase is the initial enzyme in the conversion of lysine to alpha-aminoadipic acid. We used a...
Tobin, M B, Kovacevic, S, Madduri, K, Hoskins, J A, Skatrud, P L, Vining, L C, ...
Lysine epsilon-aminotransferase (LAT) in the beta-lactam-producing actinomycetes is considered to be the first step in the antibiotic biosynthetic pathway. Cloning of restriction fragments from...
Madduri, K, Stuttard, C, Vining, L C
Genetic and biochemical evidence was obtained for lysine catabolism via cadaverine and delta-aminovalerate in both the beta-lactam producer Streptomyces clavuligerus and the nonproducer Streptomyces...
Transductional analysis of chloramphenicol biosynthesis genes in Streptomyces venezuelae.
Vats, S, Stuttard, C, Vining, L C
Auxotrophs isolated from two chloramphenicol-nonproducing mutants of Streptomyces venezuelae included three requiring pyridoxal (Pxl-), VS248 (cml-11 pdx-2), VS253 (cml-11 pdx-3), and VS258 (cml-12...
Serine utilization by Klebsiella aerogenes.
Klebsiella aerogenes was found to contain a specific L-serine dehydrase that was induced by threonine, glycine or leucine, but not by its substrate. Cellular concentrations were sensitive to carbon...
Of seven chloramphenicol-producing actinomycetes examined, only Streptomyces venezuelae strain 13s contained extrachromosomal DNA detectable by agarose gel electrophoresis and cesium...
Francis, M M, Vining, L C, Westlake, D W
In Streptomyces sp. 3022a, anthranilate synthetase is composed of two non-identical subunits. The major subunit (molecular weight, 72,000) converts chorismic acid to anthranilic acid, using ammonia...