Kaplan, D. H., Shankaran, V., Dighe, A. S., Stockert, E., Aguet, M., Old, L. J., ...
This study demonstrates that endogenously produced interferon gamma (IFN-gamma) forms the basis of a tumor surveillance system that controls development of both chemically induced and spontaneously...
Image Schemas and Lexicons: A comparison between two Lexical Networks (2004)
This paper compares the "radial category" of image schemas derived for the polysemous word "over", by Brugman and Lakoff, 1987, with the lexical networks for "over" derived by the author, from...
Effect of interleukin 12 on tumor induction by 3-methylcholanthrene.
Noguchi, Y, Jungbluth, A, Richards, E C, Old, L J
Interleukin (IL)-12 has strong antitumor activity in transplantable tumor systems in the mouse. The present study was designed to determine whether tumor induction by 3-methylcholanthrene (3-MC), a...
Chen, Y T, Stockert, E, Jungbluth, A, Tsang, S, Coplan, K A, Scanlan, M J, ...
Recent progress in the structural identification of human melanoma antigens recognized by autologous cytotoxic T cells has led to the recognition of a new melanocyte differentiation antigen,...
Immunophenotyping of melanomas for tyrosinase: implications for vaccine development.
Chen, Y T, Stockert, E, Tsang, S, Coplan, K A, Old, L J
Tyrosinase (EC 1.14.18.1), the key enzyme in melanin synthesis, has been shown to be one of the targets for cytotoxic T-cell recognition in melanoma patients. To develop serological reagents useful...
Kitamura, K, Livingston, P O, Fortunato, S R, Stockert, E, Helling, F, Ritter, G, ...
Gangliosides, such as GM2, GD2, GD3, and 9-O-acetyl GD3, are receiving attention as targets for antibody-based and vaccine-based therapies of melanoma. GM2 appears to be a particularly immunogenic...
Influence of interleukin 12 on p53 peptide vaccination against established Meth A sarcoma.
Noguchi, Y, Richards, E C, Chen, Y T, Old, L J
BALB/c murine sarcoma Meth A is known to have three missense point mutations in p53. We previously reported that a nonamer peptide containing the codon 234 mutational product (designated 234CM)...
A mouse mutant p53 product recognized by CD4+ and CD8+ T cells.
Noguchi, Y, Chen, Y T, Old, L J
The T-cell response to mutated and normal p53 products of BALB/c-derived Meth A sarcoma was analyzed. Meth A p53 is known to have three missense point mutations in codons 132, 168, and 234, and 24...
Scanlan, M J, Raj, B K, Calvo, B, Garin-Chesa, P, Sanz-Moncasi, M P, Healey, J H, ...
The human fibroblast activation protein alpha (FAP alpha) is a M(r) 95,000 cell surface antigen selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of...
Kitamura, K, Stockert, E, Garin-Chesa, P, Welt, S, Lloyd, K O, Armour, K L, ...
Le(y)-reactive monoclonal antibodies (mAbs) were generated in mice by immunization with synthetic Le(y) neoglycoproteins or with Le(y)-expressing cells. Serological analysis indicated that mAbs...
Rettig, W J, Garin-Chesa, P, Healey, J H, Su, S L, Jaffe, E A, Old, L J
Cell surface antigens of transformed cells are the traditional targets for antibody-guided detection and therapy of solid neoplasms. Alternative targets may be found in the tumor stroma, which...
Chen, Y T, Rettig, W J, Yenamandra, A K, Kozak, C A, Chaganti, R S, Posner, J B, ...
Cerebellar degeneration-related antigen (designated CDR34) was previously cloned by antibody screening of a cDNA library and was shown to be one of the target molecules recognized by autoantibodies...
Human monoclonal antibody with dual GM2/GD2 specificity derived from an immunized melanoma patient.
Yamaguchi, H, Furukawa, K, Fortunato, S R, Livingston, P O, Lloyd, K O, Oettgen, H F, ...
GM2 ganglioside is a common cell surface constituent of human melanoma and other tumors of neuroectodermal origin, and vaccination with GM2 ganglioside results in high levels of anti-GM2 antibodies...
Maki, R G, Old, L J, Srivastava, P K
Cell-surface glycoproteins of 96 kDa (gp96) have been implicated in immunogenicity of methylcholanthrene-induced mouse sarcomas in syngeneic hosts. In view of the potential immunogenicity of...
Garin-Chesa, P, Old, L J, Rettig, W J
The F19 antigen is a cell surface glycoprotein (Mr, 95,000) of human sarcomas and proliferating, cultured fibroblasts that is absent from resting fibroblasts in normal adult tissues. Normal and...
PRECIPITATING ANTIBODY IN HUMAN SERUM TO AN ANTIGEN PRESENT IN CULTURED BURKITT'S LYMPHOMA CELLS*
Old, L. J., Boyse, E. A., Oettgen, H. F., Harven, E. De, Geering, G., Williamson, B., ...
Rettig, W J, Garin-Chesa, P, Beresford, H R, Oettgen, H F, Melamed, M R, Old, L J
Normal differentiation and malignant transformation of human cells are characterized by specific changes in surface antigen phenotype. In the present study, we have defined six cell-surface antigens...
Influence of 5' flanking sequences on TL and H-2 expression in transfected L cells.
Obata, Y, Stockert, E, Chen, Y T, Takahashi, T, Old, L J
TL (thymus leukemia) antigens are encoded by genes in the major histocompatibility complex (MHC) of the mouse. Although similar in overall structure to other class I MHC antigens (H-2, Qa), TL...
Real, F X, Furukawa, K S, Mattes, M J, Gusik, S A, Cordon-Cardo, C, Oettgen, H F, ...
Antibodies present in the serum of melanoma patient FD detect an antigenic determinant (FD) restricted to the autologous melanoma cell line SK-MEL-131. This cell-surface determinant is carried on a...
Schrump, D S, Furukawa, K, Yamaguchi, H, Lloyd, K O, Old, L J
Lymph node lymphocytes from patients with primary lung cancer were immortalized with Epstein-Barr virus, and culture supernatants were screened for cell-surface reactivity against allogeneic cancer...
Furneaux, H M, Dropcho, E J, Barbut, D, Chen, Y T, Rosenblum, M K, Old, L J, ...
Paraneoplastic cerebellar degeneration is a neurological disorder of unknown cause occurring in patients with an identified or occult cancer. An autoimmune etiology is likely since autoantibodies...
Hamasima, N, Takahashi, T, Taguchi, O, Nishizuka, Y, Stockert, E, Old, L J, ...
To investigate the genetic regulation of TL expression, 12 transgenic mouse strains on a C3H (TL-nonexpressing) background have been derived: two Tg.Tlaa-3 strains with Tlaa-3 isolated from A-strain...
Cell-surface antigens of melanoma recognized by human monoclonal antibodies.
Yamaguchi, H, Furukawa, K, Fortunato, S R, Livingston, P O, Lloyd, K O, Oettgen, H F, ...
Human monoclonal antibodies (mAbs) were derived from lymph node lymphocytes and peripheral blood lymphocytes (PBL) from patients with melanoma. Four methods for generating human mAbs were compared:...
Vaccines containing purified GM2 ganglioside elicit GM2 antibodies in melanoma patients.
Livingston, P O, Natoli, E J, Calves, M J, Stockert, E, Oettgen, H F, Old, L J
GM2, GD2, and GD3 gangliosides are expressed on the surface of human melanoma cells and represent potential targets for immunological control of melanoma growth by monoclonal antibodies and active...
Chesa, P G, Rettig, W J, Melamed, M R, Old, L J, Niman, H L
Proteins encoded by cellular ras oncogenes (p21ras) are expressed in a wide variety of malignant tumors, including carcinomas, lymphomas, and neuroectodermal tumors. The function of p21ras in these...
5'-structural analysis of genes encoding polymorphic antigens of chemically induced tumors.
Srivastava, P K, Chen, Y T, Old, L J
We have proposed that the distinct tumor rejection antigens of chemically induced sarcomas in inbred mice belong to a family of Mr 96,000 glycoproteins (gp96). An identical 14-amino acid sequence was...
Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice.
Welt, S, Mattes, M J, Grando, R, Thomson, T M, Leonard, R W, Zanzonico, P B, ...
Mouse monoclonal antibody TA99 detects a 70-kDa pigmentation-associated glycoprotein in human melanoma cell lines. The antigen cannot be detected on the cell surface by sensitive rosetting techniques...
Dropcho, E J, Chen, Y T, Posner, J B, Old, L J
Autoantibodies directed against neuronal proteins have been identified in some patients with paraneoplastic cerebellar degeneration. To identify the molecular targets for these autoantibodies, we...
p53 transformation-related protein: detection of an associated phosphotransferase activity.
Jay, G, Khoury, G, DeLeo, A B, Dippold, W G, Old, L J
Malignant cells of the mouse transformed by a variety of different agents have been found to express high levels of a 53,000 Mr phosphoprotein (designated p53). Little or no p53 can be detected in...
Ueda, R, Ogata, S, Morrissey, D M, Finstad, C L, Szkudlarek, J, Whitmore, W F, ...
Seventeen monoclonal antibodies derived from fusions with spleen cells of mice immunized with established culture lines of renal cancers identified nine cell-surface antigenic systems. Six of the...
Dracopoli, N C, Rettig, W J, Whitfield, G K, Darlington, G J, Spengler, B A, Biedler, J L, ...
The chromosomal locations of the genes for the beta subunit of human thyroid-stimulating hormone (TSH) and the glycoprotein hormone alpha subunit have been determined by restriction enzyme analysis...
Cote, R J, Morrissey, D M, Houghton, A N, Thomson, T M, Daly, M E, Oettgen, H F, ...
Over 4350 human Ig-secreting hybrids have been generated through the fusion of human lymphocytes with NS-1 (mouse), LICR-2, SKO-007, GM4672, or UC729-6 (human) myeloma and lymphoblastoid cell lines....
Tumor rejection antigens of chemically induced sarcomas of inbred mice.
Srivastava, P K, DeLeo, A B, Old, L J
Chemically induced sarcomas of inbred mice are immunogenic in syngeneic hosts, and preimmunization with tumor cells leads to resistance to subsequent tumor transplants. The tumor rejection antigens...
Haranaka, K, Carswell, E A, Williamson, B D, Prendergast, J S, Satomi, N, Old, L J
Mouse tumor necrosis factor (TNF) was purified from serum through a series of steps, and each step was monitored for L-cell cytotoxicity in vitro and tumor-necrotizing activity in vivo. The two...
Partial purification of a serum factor that causes necrosis of tumors.
Green, S, Dobrjansky, A, Carswell, E A, Kassel, R L, Old, L J, Fiore, N, ...
Tumor necrosis can be induced in transplanted mouse methylcholanthrene-induced sarcoma by a tumor necrosis factor in the serum of mice infected with bacillus Calmette-Guérin and given bacterial...
Cell surface antigens of human bladder cancer defined by mouse monoclonal antibodies.
Fradet, Y, Cordon-Cardo, C, Thomson, T, Daly, M E, Whitmore, W F, Lloyd, K O, ...
Mouse monoclonal antibodies were obtained by immunization with cultured human bladder cancer or lysates of bladder papilloma. They identify 11 distinct antigenic systems as defined by serological...
Mattes, M J, Cordon-Cardo, C, Lewis, J L, Old, L J, Lloyd, K O
Mouse monoclonal antibodies to several cell surface antigens of human ovarian and endometrial carcinomas have been produced. The distribution of the antigens was determined by mixed hemagglutination...
Knuth, A, Danowski, B, Oettgen, H F, Old, L J
The cytotoxic reactivity of lymphocytes for autologous melanoma cells was studied in a group of 13 melanoma patients. No cytotoxicity was observed with lymphocytes freshly isolated from peripheral...
Mouse Lyt-2 antigen: evidence for two heterodimers with a common subunit.
Jay, G, Palladino, M A, Khoury, G, Old, L J
Immunoprecipitation of [35S]methionine-labeled extracts of BALB/c thymocytes with mouse Lyt-2.2 monoclonal antibody yielded three components with subunit Mrs of 37,000, 32,000, and 28,000 on...
Cairncross, J G, Mattes, M J, Beresford, H R, Albino, A P, Houghton, A N, Lloyd, K O, ...
The surface antigens of cultured human malignant astrocytomas were analyzed by using mouse monoclonal antibodies. BALB/c mice were immunized repeatedly with either SK-MG-1 [a glial fibrillary acidic...
Mercer, W E, Nelson, D, DeLeo, A B, Old, L J, Baserga, R
Monoclonal antibody directed against the transformation-related protein p53 was microinjected manually into the nuclei of quiescent Swiss 3T3 mouse cells. The cells were subsequently stimulated with...
Yokoyama, K, Stockert, E, Old, L J, Nathenson, S G
Comparative tryptic peptide analysis was used to probe the structure of the TL products coded for by normally expressed TL alleles (Tlaa and Tlac haplotypes) and the structure of the TL product on...
Inhibition of AKR leukemogenesis by SMX-1, a dualtropic murine leukemia virus.
Stockert, E, O'Donnell, P V, Obata, Y, Old, L J
Intrathymic injection of SMX-1, a dualtropic murine leukemia virus (MuLV) originally derived from Moloney murine leukemia virus stocks, protects AKR mice from developing MuLV-accelerated leukemia and...
Serological survey of normal humans for natural antibody to cell surface antigens of melanoma.
Houghton, A N, Taormina, M C, Ikeda, H, Watanabe, T, Oettgen, H F, Old, L J
Sera of 106 normal adult men were tested for antibodies reacting with cell surface antigens of three established lines of cultured malignant melanoma. Positive reactions with a protein A assay for...
Dippold, W G, Lloyd, K O, Li, L T, Ikeda, H, Oettgen, H F, Old, L J
Eighteen mouse monoclonal antibodies were selected for reactivity with cell surface antigens of the immunizing human melanoma cell line SK-MEL-28. Six distinct antigenic systems were defined by...
Albino, A P, Graf, L H, Kantor, R R, McLean, W, Silagi, S, Old, L J
DNA sequences encoding a human melanoma membrane-bound sialoglycoprotein of 130,000 molecular weight (gp130) were introduced into a clonal derivative of mouse B-16 melanoma cells with the selectable...
DeLeo, A B, Jay, G, Appella, E, Dubois, G C, Law, L W, Old, L J
Antisera prepared against BALB/c Meth A sarcoma in syngeneic or compatible F1 mice recognize a protein with an apparent molecular weight of 53,000 in extracts of [35S]methionine-labeled transformed...
AU cell-surface antigen of human malignant melanoma: solubilization and partial characterization.
Carey, T E, Lloyd, K O, Takahashi, T, Travassos, L R, Old, L J
AU antigen is defined by reactions of sera from patient AU with cell-surface antigens of cultured autologous melanoma cells (SK-MEL-28). Past studies established that no available cell type other...
Nakayama, E, Shiku, H, Takahashi, T, Oettgen, H F, Old, L J
BALB/c x-ray-induced leukemia RL male 1 is strongly immunogenic for (BALB/c x C57BL/6)F1 mice. Transplants of RL male 1 regressed after initial growth, and after tumor regression mice could resist...
Williamson, B D, Carswell, E A, Rubin, B Y, Prendergast, J S, Old, L J
Human cell lines of hematopoietic origin were tested for production of tumor necrosis factor (TNF). B-cell lines transformed by Epstein-Barr virus release a factor (referred to as hTNF) that is...
Effects of Dialyzable Transfer Factor in Patients with Breast Cancer
Oettgen, H. F., Old, L. J., Farrow, J. H., Valentine, F. T., Lawrence, H. S., Thomas, L.
Five patients with advanced breast cancer were treated with pooled dialyzable transfer factor from healthy adult donors. The period of treatment ranged from 21 to 310 days, the total dose from 20 to...
Protein p53 and inducer-mediated erythroleukemia cell commitment to terminal cell division.
Shen, D W, Real, F X, DeLeo, A B, Old, L J, Marks, P A, Rifkind, R A
Inducer-mediated murine erythroleukemia cell (MELC) differentiation provides a model for examining factors determining terminal cell differentiation. The nuclear protein, p53, has been implicated as...
Cell surface antigen of human neuroblastomas is related to nuclear antigen of normal cells.
Rettig, W J, Chesa, P G, Jennings, M T, Spengler, B A, Melamed, M R, Oettgen, H F, ...
The localization of MC25, an antigen first detected on the surface of human neuroblastoma cells, was determined in cultured cells and tissues. Neuroblastoma cell lines (15/17) express the antigen on...
Structural analysis of TL genes of the mouse.
Obata, Y, Chen, Y T, Stockert, E, Old, L J
Three Tla region-specific probes have been generated from the BALB/c genomic cosmid clone C6.3. One probe, pTL1, corresponds to 3' sequences of a thymus leukemia (TL)-encoding gene, whereas pTL2 and...
Purification and characterization of a human tumor necrosis factor from the LuKII cell line.
Rubin, B Y, Anderson, S L, Sullivan, S A, Williamson, B D, Carswell, E A, Old, L J
A factor with tumor necrosis factor (TNF) activity produced by the LuKII human lymphoblastoid cell line [designated TNF(LuKII)] was purified sequentially by using controlled-pore glass, lentil...
Rettig, W J, Dracopoli, N C, Goetzger, T A, Spengler, B A, Biedler, J L, Oettgen, H F, ...
The expression of 13 newly defined human cell surface antigens identified by monoclonal antibodies was studied in a panel of reduced rodent-human somatic cell hybrid clones. For each antigenic system...
A new class of murine leukemia virus associated with development of spontaneous lymphomas.
Hartley, J W, Wolford, N K, Old, L J, Rowe, W P
A new type of murine leukemia virus has been detected in thymuses of leukemic and late preleukemic AKR mice, in lymphomas developing in NIH Swiss mice carrying the AKR ectopic virus-inducing loci...
Abelson antigen: a viral tumor antigen that is also a differentiation antigen of BALB/c mice.
Risser, R, Stockert, E, Old, L J
We report here the serologic detection of a cell surface antigen common to cells transformed by the Abelson murine leukemia virus (A-MuLV) and to normal hematopoietic cells from certain strains of...
Expression of Ia-like antigens on cultured human malignant melanoma cell lines.
Winchester, R J, Wang, C Y, Gibofsky, A, Kunkel, H G, Lloyd, K O, Old, L J
Human malignant melanoma cell lines were found to bear Ia-like cell surface determinants demonstrable by hetero- or alloantisera and by direct identification of the characteristic bimolecular...
Generation of human monoclonal antibodies reactive with cellular antigens.
Cote, R J, Morrissey, D M, Houghton, A N, Beattie, E J, Oettgen, H F, Old, L J
Human lymphocytes from lymph node, peripheral blood, spleen, and tumor specimens have been fused with the LICR-LON-HMy2 (LICR-2) or SKO-007 human cell lines or the NS-1 mouse myeloma line. Over 75...
Houghton, A N, Mintzer, D, Cordon-Cardo, C, Welt, S, Fliegel, B, Vadhan, S, ...
R24 is an IgG3 mouse monoclonal antibody that identifies GD3, a prominent ganglioside on the surface of melanoma cells and other cells of neuroectodermal origin. Twelve patients with metastatic...
Dracopoli, N C, Houghton, A N, Old, L J
Loss of genetic material at certain chromosomal sites is implicated in the etiology of retinoblastoma and Wilms tumor. Whether specific chromosomal deletions are associated with other types of human...