Models and Radiation of Railgun Plasma Armatures with Non-Ideal Plasma Properties. (2002)
A theoretical framework for modeling the characteristics of railgun plasma armatures is developed taking into account non ideal transport properties and equation of state. The electrical resistivity...
The theoretical values of the transport coefficients were obtained by calculating accurate electron energy distribution functions using an assumed set of elastic and inelastic cross sections. The...
Momentum transfer cross sections for electrons in He, Ar, Kr, and Xe are obtained from a comparison of theoretical and experimental values of the drift velocities and of the ratio of the diffusion...
MOLECULAR EXCITATION OF HYDROGEN AND NITROGEN FROM ELECTRON SWARM EXPERIMENTS. (1998)
The energy distribution function of electrons in hydrogen and in nitrogen at 77 and 300K has been computed at values of the applied field to pressure ratio such that rotational excitation is the...
Anthony, K G, Kathir, P, Moore, D, Ippen-Ihler, K, Frost, L S
The sequence of a region of the F plasmid containing the traLEKBP genes involved in plasmid transfer was compared to the equivalent regions of two IncFII plasmids, R100-1 and ColB2. The traLEK gene...
The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.
Moore, D, Hamilton, C M, Maneewannakul, K, Mintz, Y, Frost, L S, Ippen-Ihler, K
The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies...
The nature of the traK4 mutation in the F sex factor of Escherichia coli.
Penfold, S S, Usher, K, Frost, L S
The sequence of traK gene of the F sex factor of Escherichia coli is presented; the traK gene product is predicted to be a protein of 25,627 Da with a signal sequence of 21 amino acids to give a...
Grossman, T H, Frost, L S, Silverman, P M
The lac-tra operon fusion plasmid pTG801 contains the known F plasmid DNA transfer (tra) genes required by Escherichia coli to elaborate functional F pili (T. Grossman and P. M. Silverman, J....
Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging.
F-like pili specified by conjugative plasmids have been reported to contain phosphate which may be noncovalently incorporated into the pilus. Electron spectroscopic imaging was able to detect...
Location of the antigenic determinants of conjugative F-like pili.
Worobec, E A, Frost, L S, Pieroni, P, Armstrong, G D, Hodges, R S, Parker, J M, ...
The amino terminus of the pilin protein constitutes the major epitope of F-like conjugative pili studied to date (F, ColB2, R1-19, R100-1, and pED208). Anti-pED208 pilus antibodies were passed...
Nucleotide sequences of five IncF plasmid finP alleles.
Finlay, B B, Frost, L S, Paranchych, W, Willetts, N S
The nucleotide sequences of five finP alleles from various IncF plasmids (finP types I to V) as well as of three finP mutations were determined and compared. The finP gene specificity could be...
Finlay, B B, Frost, L S, Paranchych, W
The complete nucleotide sequences of the ColB4-K98 (ColB4) plasmid transfer genes oriT, traM, and traY as well as the traY gene of R100-1 are presented and compared with the corresponding regions...
Frost, L S, Lee, J S, Scraba, D G, Paranchych, W
Two murine monoclonal antibodies (JEL 92 and 93) specific for adjacent epitopes on F pilin were purified and characterized. JEL 93 immunoglobulin G (IgG) and its Fab fragments were specific for the...
Nucleotide sequence of the tra YALE region from IncFV plasmid pED208.
Finlay, B B, Frost, L S, Paranchych, W
The pED208 plasmid is a 90-kilobase conjugative plasmid which is the derepressed form of Fo lac plasmid (IncFV). A 3.3-kilobase HindIII-PstI fragment from the pED208 plasmid was cloned and sequenced...
Finlay, B B, Frost, L S, Paranchych, W
The complete nucleotide sequences of the R1 drd-19 (R1-19) plasmid transfer genes traM, finP, traJ, and traY and the region encoding the traYZ promoter were determined. The traM protein from R1-19...
DNA sequence of the F traALE region that includes the gene for F pilin.
Frost, L S, Paranchych, W, Willetts, N S
The complete sequence of a 1.4-kilobase PstI fragment containing the F transfer genes traA, -L, and -E is presented. The traA reading frame has been located both genetically and by comparing the...
Localization, cloning, and sequence determination of the conjugative plasmid ColB2 pilin gene.
Finlay, B B, Frost, L S, Paranchych, W
ColB2 is a colicin-producing, 96-kilobase plasmid which encodes a conjugative system that is similar, but not identical, to F. A restriction map of this plasmid was generated, and DNA homology...
Nature of the Carbohydrate and Phosphate Associated with ColB2 and EDP208 Pilin
Armstrong, G. D., Frost, L. S., Vogel, H. J., Paranchych, W.
Studies were carried out to elucidate the nature of phosphate and sugar linkages to F-like conjugative pili encoded by the ColB2Fdr (compatibility group FII) and EDP208 (compatibility group FV)...
Major antigenic determinants of F and ColB2 pili.
Finlay, B B, Frost, L S, Paranchych, W, Parker, J M, Hodges, R S
F-like conjugative pili are expressed by plasmids with closely related transfer systems. They are tubular filaments that are composed of repeating pilin subunits arranged in a helical array. Both F...
Characterization and sequence analysis of pilin from F-like plasmids.
Frost, L S, Finlay, B B, Opgenorth, A, Paranchych, W, Lee, J S
Conjugative pili are expressed by derepressed plasmids and initiate cell-to-cell contact during bacterial conjugation. They are also the site of attachment for pilus-specific phages (f1, f2, and QB)....
N-terminal amino acid sequencing of EDP208 conjugative pili.
Frost, L S, Armstrong, G D, Finlay, B B, Edwards, B F, Paranchych, W
EDP208 conjugative pili contain a single polypeptide subunit of 11,500 daltons with a blocked N-terminus. This N-terminal blocking moiety was identified as an N-acetyl group by 1H nuclear magnetic...
N-Terminal amino acid sequence of pilin isolated from Pseudomonas aeruginosa.
Paranchych, W, Frost, L S, Carpenter, M
The amino-terminal amino acid sequence of the pili protein from Pseudomonas aeruginosa K pili is presented. The sequence is compared with those reported by others for pilin obtained from Neisseria...
Composition and molecular weight of pili purified from Pseudomonas aeruginosa K.
Pseudomonas aeruginosa strain K (PAK) bears polar pili that promote infection by at least six bacteriophages. Moreover, a recently isolated mutant of strain K (PAK/2PfS) is many times more piliated...
Comparative Biochemical Studies on F and EDP208 Conjugative Pili
Armstrong, G. D., Frost, L. S., Sastry, P. A., Paranchych, W.
EDP208 pili are encoded by a derepressed derivative of a naturally occurring lac plasmid, F0lac (incompatibility group FV), originally isolated from Salmonella typhi. EDP208 pili are serologically...
Analysis of the sequence and gene products of the transfer region of the F sex factor.
Frost, L S, Ippen-Ihler, K, Skurray, R A
Bacterial conjugation results in the transfer of DNA of either plasmid or chromosomal origin between microorganisms. Transfer begins at a defined point in the DNA sequence, usually called the origin...
Anthony, K G, Kathir, P, Moore, D, Ippen-Ihler, K, Frost, L S
The sequence of a region of the F plasmid containing the traLEKBP genes involved in plasmid transfer was compared to the equivalent regions of two IncFII plasmids, R100-1 and ColB2. The traLEK gene...
The Escherichia coli K-12 F plasmid gene traX is required for acetylation of F pilin.
Moore, D, Hamilton, C M, Maneewannakul, K, Mintz, Y, Frost, L S, Ippen-Ihler, K
The Escherichia coli F plasmid gene required for amino-terminal acetylation of F-pilin subunits was identified. Using Western blots (immunoblots), we assayed the reaction of monoclonal antibodies...
The nature of the traK4 mutation in the F sex factor of Escherichia coli.
Penfold, S S, Usher, K, Frost, L S
The sequence of traK gene of the F sex factor of Escherichia coli is presented; the traK gene product is predicted to be a protein of 25,627 Da with a signal sequence of 21 amino acids to give a...
Grossman, T H, Frost, L S, Silverman, P M
The lac-tra operon fusion plasmid pTG801 contains the known F plasmid DNA transfer (tra) genes required by Escherichia coli to elaborate functional F pili (T. Grossman and P. M. Silverman, J....
Examination of the phosphate in conjugative F-like pili by use of electron spectroscopic imaging.
F-like pili specified by conjugative plasmids have been reported to contain phosphate which may be noncovalently incorporated into the pilus. Electron spectroscopic imaging was able to detect...
Location of the antigenic determinants of conjugative F-like pili.
Worobec, E A, Frost, L S, Pieroni, P, Armstrong, G D, Hodges, R S, Parker, J M, ...
The amino terminus of the pilin protein constitutes the major epitope of F-like conjugative pili studied to date (F, ColB2, R1-19, R100-1, and pED208). Anti-pED208 pilus antibodies were passed...
Nucleotide sequences of five IncF plasmid finP alleles.
Finlay, B B, Frost, L S, Paranchych, W, Willetts, N S
The nucleotide sequences of five finP alleles from various IncF plasmids (finP types I to V) as well as of three finP mutations were determined and compared. The finP gene specificity could be...
Finlay, B B, Frost, L S, Paranchych, W
The complete nucleotide sequences of the ColB4-K98 (ColB4) plasmid transfer genes oriT, traM, and traY as well as the traY gene of R100-1 are presented and compared with the corresponding regions...
Frost, L S, Lee, J S, Scraba, D G, Paranchych, W
Two murine monoclonal antibodies (JEL 92 and 93) specific for adjacent epitopes on F pilin were purified and characterized. JEL 93 immunoglobulin G (IgG) and its Fab fragments were specific for the...
Nucleotide sequence of the tra YALE region from IncFV plasmid pED208.
Finlay, B B, Frost, L S, Paranchych, W
The pED208 plasmid is a 90-kilobase conjugative plasmid which is the derepressed form of Fo lac plasmid (IncFV). A 3.3-kilobase HindIII-PstI fragment from the pED208 plasmid was cloned and sequenced...
Finlay, B B, Frost, L S, Paranchych, W
The complete nucleotide sequences of the R1 drd-19 (R1-19) plasmid transfer genes traM, finP, traJ, and traY and the region encoding the traYZ promoter were determined. The traM protein from R1-19...
DNA sequence of the F traALE region that includes the gene for F pilin.
Frost, L S, Paranchych, W, Willetts, N S
The complete sequence of a 1.4-kilobase PstI fragment containing the F transfer genes traA, -L, and -E is presented. The traA reading frame has been located both genetically and by comparing the...
Localization, cloning, and sequence determination of the conjugative plasmid ColB2 pilin gene.
Finlay, B B, Frost, L S, Paranchych, W
ColB2 is a colicin-producing, 96-kilobase plasmid which encodes a conjugative system that is similar, but not identical, to F. A restriction map of this plasmid was generated, and DNA homology...
Nature of the Carbohydrate and Phosphate Associated with ColB2 and EDP208 Pilin
Armstrong, G. D., Frost, L. S., Vogel, H. J., Paranchych, W.
Studies were carried out to elucidate the nature of phosphate and sugar linkages to F-like conjugative pili encoded by the ColB2Fdr (compatibility group FII) and EDP208 (compatibility group FV)...
Major antigenic determinants of F and ColB2 pili.
Finlay, B B, Frost, L S, Paranchych, W, Parker, J M, Hodges, R S
F-like conjugative pili are expressed by plasmids with closely related transfer systems. They are tubular filaments that are composed of repeating pilin subunits arranged in a helical array. Both F...
Characterization and sequence analysis of pilin from F-like plasmids.
Frost, L S, Finlay, B B, Opgenorth, A, Paranchych, W, Lee, J S
Conjugative pili are expressed by derepressed plasmids and initiate cell-to-cell contact during bacterial conjugation. They are also the site of attachment for pilus-specific phages (f1, f2, and QB)....
N-terminal amino acid sequencing of EDP208 conjugative pili.
Frost, L S, Armstrong, G D, Finlay, B B, Edwards, B F, Paranchych, W
EDP208 conjugative pili contain a single polypeptide subunit of 11,500 daltons with a blocked N-terminus. This N-terminal blocking moiety was identified as an N-acetyl group by 1H nuclear magnetic...
N-Terminal amino acid sequence of pilin isolated from Pseudomonas aeruginosa.
Paranchych, W, Frost, L S, Carpenter, M
The amino-terminal amino acid sequence of the pili protein from Pseudomonas aeruginosa K pili is presented. The sequence is compared with those reported by others for pilin obtained from Neisseria...
Composition and molecular weight of pili purified from Pseudomonas aeruginosa K.
Pseudomonas aeruginosa strain K (PAK) bears polar pili that promote infection by at least six bacteriophages. Moreover, a recently isolated mutant of strain K (PAK/2PfS) is many times more piliated...
Comparative Biochemical Studies on F and EDP208 Conjugative Pili
Armstrong, G. D., Frost, L. S., Sastry, P. A., Paranchych, W.
EDP208 pili are encoded by a derepressed derivative of a naturally occurring lac plasmid, F0lac (incompatibility group FV), originally isolated from Salmonella typhi. EDP208 pili are serologically...
Analysis of the sequence and gene products of the transfer region of the F sex factor.
Frost, L S, Ippen-Ihler, K, Skurray, R A
Bacterial conjugation results in the transfer of DNA of either plasmid or chromosomal origin between microorganisms. Transfer begins at a defined point in the DNA sequence, usually called the origin...