Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca(2+)-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at...
Calcium ions are involved in Escherichia coli chemotaxis.
Escherichia coli regulates intracellular free Ca2+ at about 90 nM [Gangola, P. & Rosen, B. P. (1987) J. Biol. Chem. 262, 12570-12574]. To increase intracellular free Ca2+, nitr-5/Ca2+, a "caged" Ca2+...
Chemotactic properties of Escherichia coli mutants having abnormal Ca2+ content.
The calA, calC, and calD mutants of Escherichia coli are known to be sensitive to Ca2+ (R. N. Brey and B. P. Rosen, J. Bacteriol. 139:824-834, 1979). In the absence of any added stimuli for...
Inhibition of Escherichia coli chemotaxis by omega-conotoxin, a calcium ion channel blocker.
Tisa, L S, Olivera, B M, Adler, J
Escherichia coli chemotaxis was inhibited by omega-conotoxin, a calcium ion channel blocker. With Tris-EDTA-permeabilized cells, nanomolar levels of omega-conotoxin inhibited chemotaxis without loss...
Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase...
Isolation and nitrogenase activity of vesicles from Frankia sp. strain EAN1pec.
Vesicles, specialized cell structures thought to be the site of nitrogen fixation in the actinorhizal bacteria, were isolated from Frankia sp. strain EAN1pec by using French pressure disruption of...
Photometric immersion refractometry of bacterial spores.
Gerhardt, P, Beaman, T C, Corner, T R, Greenamyre, J T, Tisa, L S
Photometric immersion refractometry was used to determine the average apparent refractive index (n) of five types of dormant Bacillus spores representing a 600-fold range in moist-heat resistance...
Wet and dry bacterial spore densities determined by buoyant sedimentation.
Tisa, L S, Koshikawa, T, Gerhardt, P
The wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of...
Expression of glycosidase activities during germination of Dictyostelium discoideum spores.
Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of...
Identification of the metalloregulatory element of the plasmid-encoded arsenical resistance operon.
San Francisco, M J, Hope, C L, Owolabi, J B, Tisa, L S, Rosen, B P
The regulatory region of the plasmid-encoded arsenical resistance (ars) operon was cloned as a 727-bp EcoRI-HindIII fragment. When cloned into a promoter probe vector this fragment conferred arsenite...
Cytoplasmic free-Ca2+ levels in Escherichia coli were measured by use of the fluorescent Ca(2+)-indicator dye fura-2. Chemotactically wild-type E. coli regulated cytoplasmic free Ca2+ at...
Calcium ions are involved in Escherichia coli chemotaxis.
Escherichia coli regulates intracellular free Ca2+ at about 90 nM [Gangola, P. & Rosen, B. P. (1987) J. Biol. Chem. 262, 12570-12574]. To increase intracellular free Ca2+, nitr-5/Ca2+, a "caged" Ca2+...
Chemotactic properties of Escherichia coli mutants having abnormal Ca2+ content.
The calA, calC, and calD mutants of Escherichia coli are known to be sensitive to Ca2+ (R. N. Brey and B. P. Rosen, J. Bacteriol. 139:824-834, 1979). In the absence of any added stimuli for...
Inhibition of Escherichia coli chemotaxis by omega-conotoxin, a calcium ion channel blocker.
Tisa, L S, Olivera, B M, Adler, J
Escherichia coli chemotaxis was inhibited by omega-conotoxin, a calcium ion channel blocker. With Tris-EDTA-permeabilized cells, nanomolar levels of omega-conotoxin inhibited chemotaxis without loss...
Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase...
Isolation and nitrogenase activity of vesicles from Frankia sp. strain EAN1pec.
Vesicles, specialized cell structures thought to be the site of nitrogen fixation in the actinorhizal bacteria, were isolated from Frankia sp. strain EAN1pec by using French pressure disruption of...
Photometric immersion refractometry of bacterial spores.
Gerhardt, P, Beaman, T C, Corner, T R, Greenamyre, J T, Tisa, L S
Photometric immersion refractometry was used to determine the average apparent refractive index (n) of five types of dormant Bacillus spores representing a 600-fold range in moist-heat resistance...
Wet and dry bacterial spore densities determined by buoyant sedimentation.
Tisa, L S, Koshikawa, T, Gerhardt, P
The wet densities of various types of dormant bacterial spores and reference particles were determined by centrifugal buoyant sedimentation in density gradient solutions of three commercial media of...
Expression of glycosidase activities during germination of Dictyostelium discoideum spores.
Several lysosomal glycosidase activities were examined in vitro during heat-induced germination of Dictyostelium discoideum spores and were found not to be coordinately controlled. The level of...
Identification of the metalloregulatory element of the plasmid-encoded arsenical resistance operon.
San Francisco, M J, Hope, C L, Owolabi, J B, Tisa, L S, Rosen, B P
The regulatory region of the plasmid-encoded arsenical resistance (ars) operon was cloned as a 727-bp EcoRI-HindIII fragment. When cloned into a promoter probe vector this fragment conferred arsenite...