L. T. Smith

Role of osmolytes in adaptation of osmotically stressed and chill-stressed Listeria monocytogenes grown in liquid media and on processed meat surfaces.

Smith, L T

Listeria monocytogenes is a food-borne pathogen that is widely distributed in nature and is found in many kinds of fresh and processed foods. The pervasiveness of this organism is due, in part, to...

Sodium-driven, osmotically activated glycine betaine transport in Listeria monocytogenes membrane vesicles.

Gerhardt, P N, Smith, L T, Smith, G M

Transport of the osmoprotectant and cryoprotectant glycine betaine was investigated in membrane vesicles of Listeria monocytogenes. Uptake-driving transmembrane potentials ranging from 111 to 122 mV...

A prominent role for glucosylglycerol in the adaptation of Pseudomonas mendocina SKB70 to osmotic stress.

Pocard, J A, Smith, L T, Smith, G M, Le Rudulier, D

The mechanism of osmoadaptation in a salt-tolerant (1.2 M NaCl) bacterial isolate identified as Pseudomonas mendocina (N. J. Palleroni, M. Doudoroff, R. Y. Stanier, R. E. Solanes, and R. Mandel, J....

Roles of N-acetylglutaminylglutamine amide and glycine betaine in adaptation of Pseudomonas aeruginosa to osmotic stress.

D'Souza-Ault, M R, Smith, L T, Smith, G M

The mechanism of osmotic stress adaptation in Pseudomonas aeruginosa PAO1 was investigated. By using natural abundance 13C nuclear magnetic resonance spectroscopy, osmotically stressed cultures were...

Glycine betaine confers enhanced osmotolerance and cryotolerance on Listeria monocytogenes.

Ko, R, Smith, L T, Smith, G M

Listeria monocytogenes is a gram-positive food-borne pathogen that is notably resistant to osmotic stress and can grow at refrigerator temperatures. These two characteristics make it an insidious...

Characterization of three choline transport activities in Rhizobium meliloti: modulation by choline and osmotic stress.

Pocard, J A, Bernard, T, Smith, L T, Le Rudulier, D

Choline has both a nutritional and osmoregulatory role in Rhizobium meliloti (T. Bernard, J. A. Pocard, B. Perroud, and D. Le Rudulier, Arch. Microbiol. 143:359-364, 1986). In view of this fact,...

An osmoregulated dipeptide in stressed Rhizobium meliloti.

Smith, L T, Smith, G M

One common mechanism of cellular adaptation to osmotic stress is the accumulation of organic solutes in the cytosol. We have used natural-abundance 13C nuclear magnetic resonance to identify all...

Osmoregulation in Agrobacterium tumefaciens: accumulation of a novel disaccharide is controlled by osmotic strength and glycine betaine.

Smith, L T, Smith, G M, Madkour, M A

We have investigated the mechanism of osmotic stress adaptation (osmoregulation) in Agrobacterium tumefaciens biotype I (salt-tolerant) and biotype II (salt-sensitive) strains. Using...

Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti.

Smith, L T, Pocard, J A, Bernard, T, Le Rudulier, D

Intracellular accumulation of glycine betaine has been shown to confer an enhanced level of osmotic stress tolerance in Rhizobium meliloti. In this study, we used a physiological approach to...

Characterization of a gamma-glutamyl kinase from Escherichia coli that confers proline overproduction and osmotic tolerance.

Smith, L T

Mutation(s) in the proBA operon of Escherichia coli confers proline overproduction and enhanced osmotic tolerance in enteric bacteria (L. N. Csonka, Mol. Gen. Genet. 182:82-86, 1981; M. J. Mahan and...

Recombinant plasmid conferring proline overproduction and osmotic tolerance.

Jakowec, M W, Smith, L T, Dandekar, A M

A recombinant plasmid carrying the proBA (pro-74) mutant allele which governs osmotic tolerance and proline overproduction was constructed by using the broad-host-range plasmid vector pQSR49. The...

Marfan syndrome: abnormal alpha 2 chain in type I collagen.

Byers, P H, Siegel, R C, Peterson, K E, Rowe, D W, Holbrook, K A, Smith, L T, ...

Cells in culture from a woman with a variety of the Marfan syndrome produce two species of the alpha 2 chains of type I collagen. One alpha 2 chain appears normal; the abnormal chain has a higher...

A common uptake system for serotonin and dopamine in human platelets.

Omenn, G S, Smith, L T

Kinetic and pharmacologic properties of uptake of serotonin and dopamine by normal human platelets have been investigated to test whether platelets can be employed as a model system for the reuptake...

Basic fibroblast growth factor enhances the coupling of intimal hyperplasia and proliferation of vasa vasorum in injured rat arteries.

Edelman, E R, Nugent, M A, Smith, L T, Karnovsky, M J

Basic fibroblast growth factor (bFGF) is mitogenic for smooth muscle cells (SMC) and angiogenic. We examined the in vivo effects of bFGF in balloon denuded carotid arteries of laboratory rats. bFGF...

Cyclic ichthyosis with epidermolytic hyperkeratosis: A phenotype conferred by mutations in the 2B domain of keratin K1.

Sybert, V P, Francis, J S, Corden, L D, Smith, L T, Weaver, M, Stephens, K, ...

Bullous congenital ichthyosiform erythroderma (BCIE) is characterized by blistering and erythroderma in infancy and by erythroderma and ichthyosis thereafter. Epidermolytic hyperkeratosis is a...

Role of Glycine Betaine and Related Osmolytes in Osmotic Stress Adaptation in Yersinia enterocolitica ATCC 9610

Park, S., Smith, L. T., Smith, G. M.

Yersinia enterocolitica is a gram-negative, food-borne pathogen that can grow in 5% NaCl and at refrigerator temperatures. In this report, the compatible solutes (osmolytes) which accumulate...

Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen.

Smith, L T, Wertelecki, W, Milstone, L M, Petty, E M, Seashore, M R, Braverman, I M, ...

Dermatosparaxis is a recessively inherited connective-tissue disorder that results from lack of the activity of type I procollagen N-proteinase, the enzyme that removes the amino-terminal propeptides...

Role of osmolytes in adaptation of osmotically stressed and chill-stressed Listeria monocytogenes grown in liquid media and on processed meat surfaces.

Smith, L T

Listeria monocytogenes is a food-borne pathogen that is widely distributed in nature and is found in many kinds of fresh and processed foods. The pervasiveness of this organism is due, in part, to...

Sodium-driven, osmotically activated glycine betaine transport in Listeria monocytogenes membrane vesicles.

Gerhardt, P N, Smith, L T, Smith, G M

Transport of the osmoprotectant and cryoprotectant glycine betaine was investigated in membrane vesicles of Listeria monocytogenes. Uptake-driving transmembrane potentials ranging from 111 to 122 mV...

A prominent role for glucosylglycerol in the adaptation of Pseudomonas mendocina SKB70 to osmotic stress.

Pocard, J A, Smith, L T, Smith, G M, Le Rudulier, D

The mechanism of osmoadaptation in a salt-tolerant (1.2 M NaCl) bacterial isolate identified as Pseudomonas mendocina (N. J. Palleroni, M. Doudoroff, R. Y. Stanier, R. E. Solanes, and R. Mandel, J....

Roles of N-acetylglutaminylglutamine amide and glycine betaine in adaptation of Pseudomonas aeruginosa to osmotic stress.

D'Souza-Ault, M R, Smith, L T, Smith, G M

The mechanism of osmotic stress adaptation in Pseudomonas aeruginosa PAO1 was investigated. By using natural abundance 13C nuclear magnetic resonance spectroscopy, osmotically stressed cultures were...

Glycine betaine confers enhanced osmotolerance and cryotolerance on Listeria monocytogenes.

Ko, R, Smith, L T, Smith, G M

Listeria monocytogenes is a gram-positive food-borne pathogen that is notably resistant to osmotic stress and can grow at refrigerator temperatures. These two characteristics make it an insidious...

Characterization of three choline transport activities in Rhizobium meliloti: modulation by choline and osmotic stress.

Pocard, J A, Bernard, T, Smith, L T, Le Rudulier, D

Choline has both a nutritional and osmoregulatory role in Rhizobium meliloti (T. Bernard, J. A. Pocard, B. Perroud, and D. Le Rudulier, Arch. Microbiol. 143:359-364, 1986). In view of this fact,...

An osmoregulated dipeptide in stressed Rhizobium meliloti.

Smith, L T, Smith, G M

One common mechanism of cellular adaptation to osmotic stress is the accumulation of organic solutes in the cytosol. We have used natural-abundance 13C nuclear magnetic resonance to identify all...

Osmoregulation in Agrobacterium tumefaciens: accumulation of a novel disaccharide is controlled by osmotic strength and glycine betaine.

Smith, L T, Smith, G M, Madkour, M A

We have investigated the mechanism of osmotic stress adaptation (osmoregulation) in Agrobacterium tumefaciens biotype I (salt-tolerant) and biotype II (salt-sensitive) strains. Using...

Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti.

Smith, L T, Pocard, J A, Bernard, T, Le Rudulier, D

Intracellular accumulation of glycine betaine has been shown to confer an enhanced level of osmotic stress tolerance in Rhizobium meliloti. In this study, we used a physiological approach to...

Characterization of a gamma-glutamyl kinase from Escherichia coli that confers proline overproduction and osmotic tolerance.

Smith, L T

Mutation(s) in the proBA operon of Escherichia coli confers proline overproduction and enhanced osmotic tolerance in enteric bacteria (L. N. Csonka, Mol. Gen. Genet. 182:82-86, 1981; M. J. Mahan and...

Recombinant plasmid conferring proline overproduction and osmotic tolerance.

Jakowec, M W, Smith, L T, Dandekar, A M

A recombinant plasmid carrying the proBA (pro-74) mutant allele which governs osmotic tolerance and proline overproduction was constructed by using the broad-host-range plasmid vector pQSR49. The...

Marfan syndrome: abnormal alpha 2 chain in type I collagen.

Byers, P H, Siegel, R C, Peterson, K E, Rowe, D W, Holbrook, K A, Smith, L T, ...

Cells in culture from a woman with a variety of the Marfan syndrome produce two species of the alpha 2 chains of type I collagen. One alpha 2 chain appears normal; the abnormal chain has a higher...

A common uptake system for serotonin and dopamine in human platelets.

Omenn, G S, Smith, L T

Kinetic and pharmacologic properties of uptake of serotonin and dopamine by normal human platelets have been investigated to test whether platelets can be employed as a model system for the reuptake...

Basic fibroblast growth factor enhances the coupling of intimal hyperplasia and proliferation of vasa vasorum in injured rat arteries.

Edelman, E R, Nugent, M A, Smith, L T, Karnovsky, M J

Basic fibroblast growth factor (bFGF) is mitogenic for smooth muscle cells (SMC) and angiogenic. We examined the in vivo effects of bFGF in balloon denuded carotid arteries of laboratory rats. bFGF...

Cyclic ichthyosis with epidermolytic hyperkeratosis: A phenotype conferred by mutations in the 2B domain of keratin K1.

Sybert, V P, Francis, J S, Corden, L D, Smith, L T, Weaver, M, Stephens, K, ...

Bullous congenital ichthyosiform erythroderma (BCIE) is characterized by blistering and erythroderma in infancy and by erythroderma and ichthyosis thereafter. Epidermolytic hyperkeratosis is a...

Role of Glycine Betaine and Related Osmolytes in Osmotic Stress Adaptation in Yersinia enterocolitica ATCC 9610

Park, S., Smith, L. T., Smith, G. M.

Yersinia enterocolitica is a gram-negative, food-borne pathogen that can grow in 5% NaCl and at refrigerator temperatures. In this report, the compatible solutes (osmolytes) which accumulate...

Human dermatosparaxis: a form of Ehlers-Danlos syndrome that results from failure to remove the amino-terminal propeptide of type I procollagen.

Smith, L T, Wertelecki, W, Milstone, L M, Petty, E M, Seashore, M R, Braverman, I M, ...

Dermatosparaxis is a recessively inherited connective-tissue disorder that results from lack of the activity of type I procollagen N-proteinase, the enzyme that removes the amino-terminal propeptides...