lain W. Mattaj

Cofractionation of the TATA-binding protein with the RNA polymerase III transcription factor TFIIIB (1992)

Simmen, Kenneth A., Bernues, Jordi, Lewis, Joe D., Mattaj, Lain W.

We have investigated the requirement for TBP (TATA-binding protein) in transcription mediated by RNA polymerase III (pol III) in fractionated HeLa cell extracts.Two activities, TFIIIB and TFIIIC,...

Analysis of in vitro binding of U1-A protein mutants to U1 snRNA (1991)

Boelens, Wilbert, Scherly, Daniel, Jansen, Eric J.R., Kolen, Karin, Mattaj, Lain W., Van Venrooij, Walther J.

Despite the great sequence similarity between U1A and U2B″, both proteins do have a difference in RNA binding specificity and in the way they bind to their cognate RNAs. The U1A protein is able to...

A weak interaction between the U2A' protein and U2 snRNA helps to stabilize their complex with the U2B" protein (1991)

Boelens, Wilbert, Scherly, Daniel, Beijer, Ria P., Jansen, Eric J.R., Dathan, Nina A., Mattaj, Lain W., ...

The U2 snRNP complex contains two specific proteins, U2B” and U2A'. We have analysed the interaction of U2A' with U2B” and with U2 RNA. U2A' can form an weak but detectable RNA-protein complex...

Only two of the four sites of interaction with nuclear factors within the MXenopus U2 gene promoter are necessary for efficient transcription (1987)

Tebb, Graham, Bohmann, Dirk, Mattaj, Lain W.

An analysis, performed by DNase I footprinting, of the interactions between factors present in Molt-4 nuclear extracts and a Xenopus U2 snRNA gene promoter is presented. Four distinct regions of...