Lan-Ying Lee

Generation of "backbone" free, low transgene copy plants by launching T-DNA from the _Agrobacterium_ chromosome (2009)

Heiko Oltmanns, Bronwyn Frame, Lan-Ying Lee, Susan Johnson, Bo Li, Kan Wang, ...

In both applied and basic research, _Agrobacterium_-mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three _Agrobacterium tumefaciens_ strains...

Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells (2008)

Lee, Lan-Ying, Fang, Mei-Jane, Kuang, Lin-Yun, Gelvin, Stanton B

Abstract Background The investigation of protein-protein interactions is important for characterizing protein function. Bimolecular fluorescence complementation (BiFC) has recently gained interest as...

The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA (2003)

Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.

The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four...

pSa Causes Oncogenic Suppression of Agrobacterium by Inhibiting VirE2 Protein Export

Lee, Lan-Ying, Gelvin, Stanton B., Kado, Clarence I.

When coresident with the Ti (tumor-inducing) plasmid, the 21-kDa product of the osa gene of the plasmid pSa can suppress crown gall tumorigenesis incited by Agrobacterium tumefaciens. Neither T-DNA...

The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA

Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.

The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four ATP-dependent...

Identification of Arabidopsis rat Mutants

Zhu, Yanmin, Nam, Jaesung, Humara, Jaime M., Mysore, Kirankumar S., Lee, Lan-Ying, Cao, Hongbin, ...

Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to...

Characterization of the Arabidopsis Lysine-Rich Arabinogalactan-Protein AtAGP17 Mutant (rat1) That Results in a Decreased Efficiency of Agrobacterium Transformation1[w]

Gaspar, Yolanda Maria, Nam, Jaesung, Schultz, Carolyn Jane, Lee, Lan-Ying, Gilson, Paul R., Gelvin, Stanton B., ...

Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens...

Osa Protein Constitutes a Strong Oncogenic Suppression System That Can Block vir-Dependent Transfer of IncQ Plasmids between Agrobacterium Cells and the Establishment of IncQ Plasmids in Plant Cells

Lee, Lan-Ying, Gelvin, Stanton B.

The osa (oncogenic suppressive activity) gene of the IncW group plasmid pSa is sufficient to suppress tumorigenesis by Agrobacterium tumefaciens. osa confers oncogenic suppression by inhibiting VirE2...

pSa Causes Oncogenic Suppression of Agrobacterium by Inhibiting VirE2 Protein Export

Lee, Lan-Ying, Gelvin, Stanton B., Kado, Clarence I.

When coresident with the Ti (tumor-inducing) plasmid, the 21-kDa product of the osa gene of the plasmid pSa can suppress crown gall tumorigenesis incited by Agrobacterium tumefaciens. Neither T-DNA...

The Arabidopsis AtLIG4 gene is required for the repair of DNA damage, but not for the integration of Agrobacterium T-DNA

Van Attikum, Haico, Bundock, Paul, Overmeer, René M., Lee, Lan-Ying, Gelvin, Stanton B., Hooykaas, Paul J. J.

The joining of breaks in the chromosomal DNA backbone by ligases in processes of replication, recombination and repair plays a crucial role in the maintenance of genomic stability. Four ATP-dependent...

Identification of Arabidopsis rat Mutants

Zhu, Yanmin, Nam, Jaesung, Humara, Jaime M., Mysore, Kirankumar S., Lee, Lan-Ying, Cao, Hongbin, ...

Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to...

Characterization of the Arabidopsis Lysine-Rich Arabinogalactan-Protein AtAGP17 Mutant (rat1) That Results in a Decreased Efficiency of Agrobacterium Transformation1[w]

Gaspar, Yolanda Maria, Nam, Jaesung, Schultz, Carolyn Jane, Lee, Lan-Ying, Gilson, Paul R., Gelvin, Stanton B., ...

Arabinogalactan-proteins (AGPs) are a family of complex proteoglycans widely distributed in plants. The Arabidopsis rat1 mutant, previously characterized as resistant to Agrobacterium tumefaciens...

Osa Protein Constitutes a Strong Oncogenic Suppression System That Can Block vir-Dependent Transfer of IncQ Plasmids between Agrobacterium Cells and the Establishment of IncQ Plasmids in Plant Cells

Lee, Lan-Ying, Gelvin, Stanton B.

The osa (oncogenic suppressive activity) gene of the IncW group plasmid pSa is sufficient to suppress tumorigenesis by Agrobacterium tumefaciens. osa confers oncogenic suppression by inhibiting VirE2...

Vectors for multi-color bimolecular fluorescence complementation to investigate protein-protein interactions in living plant cells

Lee, Lan-Ying, Fang, Mei-Jane, Kuang, Lin-Yun, Gelvin, Stanton B

Novel Plant Transformation Vectors Containing the Superpromoter1[OA]

Lee, Lan-Ying, Kononov, Maria E., Bassuner, Burgund, Frame, Bronwyn R., Wang, Kan, Gelvin, Stanton B.

We developed novel plasmids and T-DNA binary vectors that incorporate a modified and more useful form of the superpromoter. The superpromoter consists of a trimer of the octopine synthase...

T-DNA Binary Vectors and Systems

Lee, Lan-Ying, Gelvin, Stanton B.

The Agrobacterium rhizogenes GALLS Gene Encodes Two Secreted Proteins Required for Genetic Transformation of Plants▿

Hodges, Larry D., Lee, Lan-Ying, McNett, Henry, Gelvin, Stanton B., Ream, Walt

Agrobacterium tumefaciens and Agrobacterium rhizogenes are related pathogens that cause crown gall and hairy root diseases, which result from integration and expression of bacterial genes in the...

IMPa-4, an Arabidopsis Importin α Isoform, Is Preferentially Involved in Agrobacterium-Mediated Plant Transformation[W]

Bhattacharjee, Saikat, Lee, Lan-Ying, Oltmanns, Heiko, Cao, Hongbin, Cuperus, Joshua, ...

Successful transformation of plants by Agrobacterium tumefaciens requires that the bacterial T-complex actively escorts T-DNA into the host's nucleus. VirD2 and VirE2 are virulence proteins on the...