Lane Barksdale

Chemical Characterization of Organisms Isolated from Leprosy Patients

Beaman, Blain L., Kim, Kwang-Shin, Lanéelle, Marie A., Barksdale, Lane

Chemical analyses of the cell walls of organisms isolated in various parts of the world from cases of lepromatous and tuberculoid leprosy make possible their assignment to one of the three genera:...

Phenoloxidase Activity in Organisms Isolated from Lepromatous and Tuberculoid Leprosy

Beaman, LoVelle, Barksdale, Lane

Anaerobic corynebacteria (propionibacteria), isolated in pure culture from the plasma of a case of tuberculoid leprosy and from lepromata of cases of lepromatous leprosy, exhibited phenoloxidase...

Park-Williams Number 8 Strain of Corynebacterium diphtheriae

Lampidis, Theodore, Barksdale, Lane

Five clones of the Park-Williams number 8 strain of Corynebacterium diphtheriae, previously maintained in separate laboratories, were examined for their colonial and biochemical properties, for the...

Ultrastructural Changes in Bacteria Isolated from Cases of Leprosy

Freer, John, Kim, Kwang S., Krauss, M. R., Beaman, Lovelle, Barksdale, Lane

One of two groups of rod-shaped bacteria (bacilli) isolated from cases of human leprosy have been found, under certain cultural conditions, to give rise to unusual phenotypes. In electron micrographs...

Distribution of Neuraminidase and N-Acetylneuraminate Lyase Activities Among Corynebacteria, Mycobacteria, and Nocardias

Arden, Sheldon B., Chang, Woo-Hyun, Barksdale, Lane

In Corynebacterium diphtheriae and closely related neuraminidase-producing corynebacteria, we have found an N-acetylneuraminate (NAN) lyase activity which cleaves NAN into N-acetyl-d-mannosamine and,...

Cytochemical Reactions of Human Leprosy Bacilli and Mycobacteria: Ultrastructural Implications

Fisher, Clark A., Barksdale, Lane

Leprosy bacilli harvested from freshly biopsied tissue from cases of lepromatous, borderline and histoid leprosy were, in conjunction with Mycobacterium lepraemurium and representative mycobacteria,...

System for the Investigation of the Bacteriophage-directed Synthesis of Diphtherial Toxin

Matsuda, Morihiro, Barksdale, Lane

Lytic corynebacteriophage βhv64tox+ has been characterized, and methods for studying the expression of its tox+ gene in nontoxinogenic Corynebacterium diphtheriae strain C7s(−)tox− described....

Neuraminidase of Corynebacterium diphtheriae

Moriyama, Takafumi, Barksdale, Lane

Neuraminidase activity has been found in a variety of strains of Corynebacterium diphtheriae, both toxinogenic and nontoxinogenic. The enzyme has been shown to be intracellular, possibly associated...

Elimination of the Acid Fastness but not the Gram Positivity of Leprosy Bacilli After Extraction with Pyridine

Fisher, Clark A., Barksdale, Lane

Bacilli from a number of tissues excised from cases of lepromatous and borderline leprosy, unlike mycobacteria, were found to lose their acid-fastness after treatment with pyridine.

Crystalline Inclusions of Bacterium 22M

Kim, Kwang S., Barksdale, Lane

Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows.

Genetic Analysis of tox+ and tox− Bacteriophages of Corynebacterium diphtheriae1

Holmes, Randall K., Barksdale, Lane

A series of mutants derived from the temperate corynebacteriophages βtox+, γtox−, and Ltox+ was isolated and characterized. In three-factor crosses between mutant β phages the relative map order...

Comparative Studies with tox+ and tox− Corynebacteriophages 1

Holmes, Randall K., Barksdale, Lane

The characteristics of nine inducible temperate corynebacteriophages designated αtox+, βtox+, Ptox+, γtox−, πtox+, Ktox−, ρtox−, Ltox+, and δtox+ have been compared. Virion morphology and...

Chemical Characterization of Organisms Isolated from Leprosy Patients

Beaman, Blain L., Kim, Kwang-Shin, Lanéelle, Marie A., Barksdale, Lane

Chemical analyses of the cell walls of organisms isolated in various parts of the world from cases of lepromatous and tuberculoid leprosy make possible their assignment to one of the three genera:...

Phenoloxidase Activity in Organisms Isolated from Lepromatous and Tuberculoid Leprosy

Beaman, LoVelle, Barksdale, Lane

Anaerobic corynebacteria (propionibacteria), isolated in pure culture from the plasma of a case of tuberculoid leprosy and from lepromata of cases of lepromatous leprosy, exhibited phenoloxidase...

Park-Williams Number 8 Strain of Corynebacterium diphtheriae

Lampidis, Theodore, Barksdale, Lane

Five clones of the Park-Williams number 8 strain of Corynebacterium diphtheriae, previously maintained in separate laboratories, were examined for their colonial and biochemical properties, for the...

Ultrastructural Changes in Bacteria Isolated from Cases of Leprosy

Freer, John, Kim, Kwang S., Krauss, M. R., Beaman, Lovelle, Barksdale, Lane

One of two groups of rod-shaped bacteria (bacilli) isolated from cases of human leprosy have been found, under certain cultural conditions, to give rise to unusual phenotypes. In electron micrographs...

Distribution of Neuraminidase and N-Acetylneuraminate Lyase Activities Among Corynebacteria, Mycobacteria, and Nocardias

Arden, Sheldon B., Chang, Woo-Hyun, Barksdale, Lane

In Corynebacterium diphtheriae and closely related neuraminidase-producing corynebacteria, we have found an N-acetylneuraminate (NAN) lyase activity which cleaves NAN into N-acetyl-d-mannosamine and,...

Cytochemical Reactions of Human Leprosy Bacilli and Mycobacteria: Ultrastructural Implications

Fisher, Clark A., Barksdale, Lane

Leprosy bacilli harvested from freshly biopsied tissue from cases of lepromatous, borderline and histoid leprosy were, in conjunction with Mycobacterium lepraemurium and representative mycobacteria,...

System for the Investigation of the Bacteriophage-directed Synthesis of Diphtherial Toxin

Matsuda, Morihiro, Barksdale, Lane

Lytic corynebacteriophage βhv64tox+ has been characterized, and methods for studying the expression of its tox+ gene in nontoxinogenic Corynebacterium diphtheriae strain C7s(−)tox− described....

Neuraminidase of Corynebacterium diphtheriae

Moriyama, Takafumi, Barksdale, Lane

Neuraminidase activity has been found in a variety of strains of Corynebacterium diphtheriae, both toxinogenic and nontoxinogenic. The enzyme has been shown to be intracellular, possibly associated...

Elimination of the Acid Fastness but not the Gram Positivity of Leprosy Bacilli After Extraction with Pyridine

Fisher, Clark A., Barksdale, Lane

Bacilli from a number of tissues excised from cases of lepromatous and borderline leprosy, unlike mycobacteria, were found to lose their acid-fastness after treatment with pyridine.

Crystalline Inclusions of Bacterium 22M

Kim, Kwang S., Barksdale, Lane

Crystalline inclusions occur in bacterium 22M. Arrays consist of parallel rows of polyhedral subunits (7.5 nm in diameter), 2.0 nm apart, with a distance of 3.0 nm between rows.

Genetic Analysis of tox+ and tox− Bacteriophages of Corynebacterium diphtheriae1

Holmes, Randall K., Barksdale, Lane

A series of mutants derived from the temperate corynebacteriophages βtox+, γtox−, and Ltox+ was isolated and characterized. In three-factor crosses between mutant β phages the relative map order...

Comparative Studies with tox+ and tox− Corynebacteriophages 1

Holmes, Randall K., Barksdale, Lane

The characteristics of nine inducible temperate corynebacteriophages designated αtox+, βtox+, Ptox+, γtox−, πtox+, Ktox−, ρtox−, Ltox+, and δtox+ have been compared. Virion morphology and...