Larry Gold

ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome (2007)

Taussig, Michael J., Stoevesandt, Oda, Bradbury, Andrew R., Cahill, Dolores, Cambillau, Christian, ...

ProteomeBinders is a new European consortium aiming to establish a comprehensive resource of well-characterized affinity reagents, including but not limited to antibodies, for analysis of the human...

Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX (2000)

Shtatland, Timur, Gill, Stanley C., Javornik, Brenda E., Johansson, Hans E., Singer, Britta S., Uhlenbeck, Olke C., ...

Genomic SELEX is a method for studying the network of nucleic acid–protein interactions within any organism. Here we report the discovery of several interesting and potentially biologically...

RNA ligands to human nerve growth factor (1995)

Binkley, Jonathan, Allen, Patrick, Brown, David M., Green, Louis, Tuerk, Craig, Gold, Larry

High affinity RNA ligands to human nerve growth factor (NGF) were selected from pools of random RNA using SELEX [Tuerk, C. and Gold, L. (1990) Science, 249, 505-510]. Nerve growth factor, which is a...

Quantitative analysis of ribosome binding sites in E.coli (1994)

Barrick, Doug, Villanueba, Keith, Childs, John, Kalil, Rhonda, Schneider, Thomas D., Lawrence, Charles E., ...

185 clones with randomized ribosome binding sites, from position −11 to 0 preceding the coding region of β-galactosidase, were selected and sequenced. The translatlonal yield of each clone was...

Binding of the bacteriophage T4 regA protein to mRNA targets: an initiator AUG is required (1990)

Unnithan, Shashikala, Green, Louis, Morrissey, Louisa, Binkley, Jonathan, Singer, Britta, Karam, Jim, ...

Bacteriophage T4 regA protein translationally represses the synthesis of a subset of early phage-induced proteins. The protein binds to the translation initiation site of at least two mRNAs and...

Quantitative analysis of the relationship between nucleotide sequence and functional activity (1986)

Stormo, Gary D., Schneider, Thomas D., Gold, Larry

Matrices can be used to evaluate sequences for functional activity. Multiple regression can solve for the matrix that gives the best fit between sequence evaluations and quantitative activities. This...

Translationsl regulation of expression of the bacteriophage T4 lysozyme gene (1986)

McPheeters, David S., Christensen, Alan, Young, Elton T., Stormo, Gary, Gold, Larry

The bacteriophage T4 lysozyme gene is transcribed at early and late times after infection of E. coli, but the early mRNA is not translated. DNA sequence analysis and mapping of the 5′ ends of the...

The bacteriophage T4 regA gene: primary sequence of a translational repressor (1984)

Trojanowska, Maria, Miller, Eric S., Karam, Jim, Stormo, Gary, Gold, Larry

The regA gene product of bacteriophage T4 is an autogenously controlled translational regulatory protein that plays a role In differential inhibition (translational repression) of a subpopulation of...

Use of the 'Perceptron' algorithm to distinguish translational initiation sites in E. coli (1982)

Stormo, Gary D., Schneider, Thomas D., Gold, Larry, Ehrenfeucht, Andrzej

We have used a “Perceptron” algorithm to find a weighting function which distinguishes E. coli translational initiation sites from all other sites in a library of over 78,000 nucleotides of mRNA...

A design for computer nucleic-acid-sequence storage, retrieval, and manipulation (1982)

Schneider, Thomas D., Stormo, Gary D., Haemer, Jeffrey S., Gold, Larry

He have designed and built a data-base system for the storage of nucleic-acid sequences. The system consists of a data base (“the library”) and software that manages and provides access to that...

From oligonucleotide shapes to genomic SELEX: Novel biological regulatory loops

Gold, Larry, Brown, David, He, Yi-yuan, Shtatland, Timur, Singer, Britta S., Wu, Yan

The SELEX method and oligonucleotide combinatorial chemistry discovery process yields high-affinity/high-specificity ligands for virtually any molecular target. Typically, the enormous starting...

High affinity ligands from in vitro selection: Complex targets

Morris, Kevin N., Jensen, Kirk B., Julin, Carol M., Weil, Michael, Gold, Larry

Human red blood cell membranes were used as a model system to determine if the systematic evolution of ligands by exponential enrichment (SELEX) methodology, an in vitro protocol for isolating...

In vitro selection of self-cleaving RNAs with a low pH optimum

Jayasena, Vineetha K., Gold, Larry

RNAs that undergo a rapid site-specific cleavage at low pH have been selected by in vitro selection (the SELEX process). The cleavage does not require the addition of any divalent metal ions, and is...

Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX

Shtatland, Timur, Gill, Stanley C., Javornik, Brenda E., Johansson, Hans E., Singer, Britta S., Uhlenbeck, Olke C., ...

Genomic SELEX is a method for studying the network of nucleic acid–protein interactions within any organism. Here we report the discovery of several interesting and potentially biologically...

A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment

Daniels, Dion A., Chen, Hang, Hicke, Brian J., Swiderek, Kristine M., Gold, Larry

The targeting of molecular repertoires to complex systems rather than biochemically pure entities is an accessible approach that can identify proteins of biological interest. We have probed antigens...

A design for computer nucleic-acid-sequence storage, retrieval, and manipulation

Schneider, Thomas D., Stormo, Gary D., Haemer, Jeffrey S., Gold, Larry

We have designed and built a data-base system for the storage of nucleic-acid sequences. The system consists of a data base (“the library”) and software that manages and provides access to that...

From oligonucleotide shapes to genomic SELEX: Novel biological regulatory loops

Gold, Larry, Brown, David, He, Yi-yuan, Shtatland, Timur, Singer, Britta S., Wu, Yan

The SELEX method and oligonucleotide combinatorial chemistry discovery process yields high-affinity/high-specificity ligands for virtually any molecular target. Typically, the enormous starting...

High affinity ligands from in vitro selection: Complex targets

Morris, Kevin N., Jensen, Kirk B., Julin, Carol M., Weil, Michael, Gold, Larry

Human red blood cell membranes were used as a model system to determine if the systematic evolution of ligands by exponential enrichment (SELEX) methodology, an in vitro protocol for isolating...

In vitro selection of self-cleaving RNAs with a low pH optimum

Jayasena, Vineetha K., Gold, Larry

RNAs that undergo a rapid site-specific cleavage at low pH have been selected by in vitro selection (the SELEX process). The cleavage does not require the addition of any divalent metal ions, and is...

Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX

Shtatland, Timur, Gill, Stanley C., Javornik, Brenda E., Johansson, Hans E., Singer, Britta S., Uhlenbeck, Olke C., ...

Genomic SELEX is a method for studying the network of nucleic acid–protein interactions within any organism. Here we report the discovery of several interesting and potentially biologically...

A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment

Daniels, Dion A., Chen, Hang, Hicke, Brian J., Swiderek, Kristine M., Gold, Larry

The targeting of molecular repertoires to complex systems rather than biochemically pure entities is an accessible approach that can identify proteins of biological interest. We have probed antigens...

A design for computer nucleic-acid-sequence storage, retrieval, and manipulation

Schneider, Thomas D., Stormo, Gary D., Haemer, Jeffrey S., Gold, Larry

We have designed and built a data-base system for the storage of nucleic-acid sequences. The system consists of a data base (“the library”) and software that manages and provides access to that...