Thomas, Sara H., Wagner, Ryan D., Arakaki, Adrian K., Skolnick, Jeffrey, Kirby, John R., Shimkets, Lawrence J., ...
©2008 Thomas et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any...
Myxococcus xanthus fibril appendages are essential for excitation by a phospholipid attractant
Kearns, Daniel B., Campbell, Blakemore D., Shimkets, Lawrence J.
Isolated (A-motile) Myxococcus xanthus cells glide over solid surfaces and display excitation, a suppression of direction reversals, when presented with phosphatidylethanolamine (PE) purified from...
Chemotaxis in a gliding bacterium
Kearns, Daniel B., Shimkets, Lawrence J.
Myxococcus xanthus cells exhibit directed motility up phosphatidylethanolamine (PE) gradients, and we suggest that PE behaves as a chemoattractant. Computer-assisted stop-motion digital microscopy...
Kearns, Daniel B., Venot, Andre, Bonner, Pamela J., Stevens, Brian, Boons, Geert-Jan, Shimkets, Lawrence J.
Fruiting body formation of Myxococcus xanthus requires the ordered migration of tens of thousands of cells by using a form of surface motility known as gliding and chemical signal(s) that have yet to...
Wise, Mark G., McArthur, J Vaun, Shimkets, Lawrence J.
The diversity of the methanotrophic community in mildly acidic landfill cover soil was assessed by three methods: two culture-independent molecular approaches and a traditional culture-based...
Yang, Zhaomin, Ma, Xiaoyuan, Tong, Leming, Kaplan, Heidi B., Shimkets, Lawrence J., Shi, Wenyuan
Myxococcus xanthus social (S) gliding motility has been previously reported by us to require the chemotaxis homologues encoded by the dif genes. In addition, two cell surface structures, type IV pili...
Pseudomonas aeruginosa Exhibits Directed Twitching Motility Up Phosphatidylethanolamine Gradients
Kearns, Daniel B., Robinson, Jayne, Shimkets, Lawrence J.
Pseudomonas aeruginosa translocates over solid surfaces by a type IV pilus-dependent form of multicellular motility known as twitching. We wondered whether cells utilize endogenous factors to...
Mapping of Myxococcus xanthus Social Motility dsp Mutations to the dif Genes
Lancero, Hope, Brofft, Jennifer E., Downard, John, Birren, Bruce W., Nusbaum, Chad, Naylor, Jerome, ...
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping,...
Kearns, Daniel B., Bonner, Pamela J., Smith, Daniel R., Shimkets, Lawrence J.
An extracellular matrix connects bacteria that live in organized assemblages called biofilms. While the role of the matrix in the regulation of cell behavior has not been extensively examined in...
Simunovic, Vesna, Gherardini, Frank C., Shimkets, Lawrence J.
Myxococcus xanthus cells coordinate cellular motility, biofilm formation, and development through the use of cell signaling pathways. In an effort to understand the mechanisms underlying these...
The stringent response in Myxococcus xanthus is regulated by SocE and the CsgA C-signaling protein
Crawford, Eugene W., Shimkets, Lawrence J.
Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We...
Developmental cell interactions in Myxococcus xanthus and the spoC locus
Shimkets, Lawrence J., Gill, Ronald E., Kaiser, Dale
The product(s) of the Myxococcus xanthus spoC locus is required for two multicellular activities in fruiting body development, rippling and sporulation. Ripples, which are formed early in...
Cohesion-Defective Mutants of Myxococcus xanthus
Bonner, Pamela J., Shimkets, Lawrence J.
Cohesion of Myxococcus xanthus cells involves interaction of a cell surface cohesin with a component of the extracellular matrix. In this work, two previously isolated cohesion-defective (fbd)...
Myxococcus xanthus fibril appendages are essential for excitation by a phospholipid attractant
Kearns, Daniel B., Campbell, Blakemore D., Shimkets, Lawrence J.
Isolated (A-motile) Myxococcus xanthus cells glide over solid surfaces and display excitation, a suppression of direction reversals, when presented with phosphatidylethanolamine (PE) purified from...
Chemotaxis in a gliding bacterium
Kearns, Daniel B., Shimkets, Lawrence J.
Myxococcus xanthus cells exhibit directed motility up phosphatidylethanolamine (PE) gradients, and we suggest that PE behaves as a chemoattractant. Computer-assisted stop-motion digital microscopy...
Kearns, Daniel B., Venot, Andre, Bonner, Pamela J., Stevens, Brian, Boons, Geert-Jan, Shimkets, Lawrence J.
Fruiting body formation of Myxococcus xanthus requires the ordered migration of tens of thousands of cells by using a form of surface motility known as gliding and chemical signal(s) that have yet to...
Wise, Mark G., McArthur, J Vaun, Shimkets, Lawrence J.
The diversity of the methanotrophic community in mildly acidic landfill cover soil was assessed by three methods: two culture-independent molecular approaches and a traditional culture-based...
Yang, Zhaomin, Ma, Xiaoyuan, Tong, Leming, Kaplan, Heidi B., Shimkets, Lawrence J., Shi, Wenyuan
Myxococcus xanthus social (S) gliding motility has been previously reported by us to require the chemotaxis homologues encoded by the dif genes. In addition, two cell surface structures, type IV pili...
Pseudomonas aeruginosa Exhibits Directed Twitching Motility Up Phosphatidylethanolamine Gradients
Kearns, Daniel B., Robinson, Jayne, Shimkets, Lawrence J.
Pseudomonas aeruginosa translocates over solid surfaces by a type IV pilus-dependent form of multicellular motility known as twitching. We wondered whether cells utilize endogenous factors to...
Mapping of Myxococcus xanthus Social Motility dsp Mutations to the dif Genes
Lancero, Hope, Brofft, Jennifer E., Downard, John, Birren, Bruce W., Nusbaum, Chad, Naylor, Jerome, ...
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping,...
Kearns, Daniel B., Bonner, Pamela J., Smith, Daniel R., Shimkets, Lawrence J.
An extracellular matrix connects bacteria that live in organized assemblages called biofilms. While the role of the matrix in the regulation of cell behavior has not been extensively examined in...
Simunovic, Vesna, Gherardini, Frank C., Shimkets, Lawrence J.
Myxococcus xanthus cells coordinate cellular motility, biofilm formation, and development through the use of cell signaling pathways. In an effort to understand the mechanisms underlying these...
The stringent response in Myxococcus xanthus is regulated by SocE and the CsgA C-signaling protein
Crawford, Eugene W., Shimkets, Lawrence J.
Myxococcus xanthus fruiting body development is induced by amino acid limitation. The decision to grow or develop is established by the RelA-dependent stringent response and A-signaling. We...
Developmental cell interactions in Myxococcus xanthus and the spoC locus
Shimkets, Lawrence J., Gill, Ronald E., Kaiser, Dale
The product(s) of the Myxococcus xanthus spoC locus is required for two multicellular activities in fruiting body development, rippling and sporulation. Ripples, which are formed early in...
Cohesion-Defective Mutants of Myxococcus xanthus
Bonner, Pamela J., Shimkets, Lawrence J.
Cohesion of Myxococcus xanthus cells involves interaction of a cell surface cohesin with a component of the extracellular matrix. In this work, two previously isolated cohesion-defective (fbd)...
Avadhani, Madhavi, Geyer, Roland, White, David C., Shimkets, Lawrence J.
Short-chain alcohol dehydrogenases (SCADHs) synthesize a variety of intercellular signals and other chemically diverse products. It is difficult to predict the substrate of a SCADH on the basis of...
Lipolytic Enzymes in Myxococcus xanthus▿
Moraleda-Muñoz, Aurelio, Shimkets, Lawrence J.
The genome of Myxococcus xanthus encodes lipolytic enzymes in three different families: patatin lipases, α/β hydrolases, and GDSL lipases. One member of each family was characterized. The protein...
Corby-Harris, Vanessa, Pontaroli, Ana Clara, Shimkets, Lawrence J., Bennetzen, Jeffrey L., Habel, Kristin E., Promislow, Daniel E. L.
Drosophila melanogaster is one of the most widely used model systems in biology. However, little is known about its associated bacterial community. As a first step towards understanding these...
Proteins Associated with the Myxococcus xanthus Extracellular Matrix▿ †
Curtis, Patrick D., Atwood, James, Orlando, Ron, Shimkets, Lawrence J.
Fruiting body formation of Myxococcus xanthus, like biofilm formation of many other organisms, involves the production of an extracellular matrix (ECM). While the polysaccharide component has been...
Spatial Organization of Myxococcus xanthus during Fruiting Body Formation▿ †
Curtis, Patrick D., Taylor, Rion G., Welch, Roy D., Shimkets, Lawrence J.
Microcinematography was used to examine fruiting body development of Myxococcus xanthus. Wild-type cells progress through three distinct phases: a quiescent phase with some motility but little...
Thomas, Sara H., Wagner, Ryan D., Arakaki, Adrian K., Skolnick, Jeffrey, Kirby, John R., Shimkets, Lawrence J., ...
Anaeromyxobacter dehalogenans strain 2CP-C is a versaphilic delta-Proteobacterium distributed throughout many diverse soil and sediment environments. 16S rRNA gene phylogenetic analysis groups A....
Bagwell, Christopher E., Bhat, Swapna, Hawkins, Gary M., Smith, Bryan W., Biswas, Tapan, Hoover, Timothy R., ...
Kineococcus radiotolerans SRS30216 was isolated from a high-level radioactive environment at the Savannah River Site (SRS) and exhibits γ-radiation resistance approaching that of Deinococcus...