Leann Tilley

Targeted mutagenesis of the ring-exported protein-1 of Plasmodium falciparum disrupts the architecture of Maurer's cleft organelles (2008)

Hanssen, Eric, Hawthorne, Paula, Dixon, Matthew W. A., Trenholme, Katharine R, McMillan, Paul J., Spielmann, Tobias, ...

Mature red blood cells have no internal trafficking machinery, so the intraerythrocytic malaria parasite, Plasmodium falciparum, establishes its own transport system to export virulence factors to...

Codes for PEXEL-negative and PEXEL-positive Proteins □D □V Exported into the Host Cell (2006)

Tobias Spielmann, Paula L. Hawthorne, Y Hannemann, Kathleen Klotz, David J. Kemp, ...

Blood stages of Plasmodium falciparum export proteins into their erythrocyte host, thereby inducing extensive host cell modifications that become apparent after the first half of the asexual...

Erythrocyte membrane vesiculation: Model for the molecular mechanism of protein sorting

Knowles, David W., Tilley, Leann, Mohandas, Narla, Chasis, Joel Anne

Budding and vesiculation of erythrocyte membranes occurs by a process involving an uncoupling of the membrane skeleton from the lipid bilayer. Vesicle formation provides an important means whereby...

Specific Inhibition of the Eubacterial DNA Ligase by Arylamino Compounds

Ciarrocchi, Giovanni, MacPhee, Donald G., Deady, Les W., Tilley, Leann

All known DNA ligases catalyze the formation of a phosphodiester linkage between adjacent termini in double-stranded DNA via very similar mechanisms. The ligase family can, however, be divided into...

Isolation of Peptides That Mimic Epitopes on a Malarial Antigen from Random Peptide Libraries Displayed on Phage

Adda, Christopher G., Tilley, Leann, Anders, Robin F., Foley, Michael

The ring-infected erythrocyte surface antigen (RESA) is a dense-granule protein of Plasmodium falciparum which binds to the cytoskeletal structure of the erythrocyte after parasite invasion. It is...

Trafficking and assembly of the cytoadherence complex in Plasmodium falciparum-infected human erythrocytes

Wickham, Mark E., Rug, Melanie, Ralph, Stuart A., Klonis, Nectarios, McFadden, Geoffrey I., Tilley, Leann, ...

After invading human erythrocytes, the malarial parasite Plasmodium falciparum, initiates a remarkable process of secreting proteins into the surrounding erythrocyte cytoplasm and plasma membrane....

Correct Promoter Control Is Needed for Trafficking of the Ring-Infected Erythrocyte Surface Antigen to the Host Cytosol in Transfected Malaria Parasites

Rug, Melanie, Wickham, Mark E., Foley, Michael, Cowman, Alan F., Tilley, Leann

Following invasion of human erythrocytes, the malaria parasite, Plasmodium falciparum, exports proteins beyond the confines of its own plasma membrane to modify the properties of the host red cell...

Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains

Frankland, Sarah, Adisa, Akinola, Horrocks, Paul, Taraschi, Theodore F., Schneider, Timothy, Elliott, Salenna R., ...

The particular virulence of the human malaria parasite Plasmodium falciparum derives from export of parasite-encoded proteins to the surface of the mature erythrocytes in which it resides. The...

Genesis of and Trafficking to the Maurer's Clefts of Plasmodium falciparum-Infected Erythrocytes†

Spycher, Cornelia, Rug, Melanie, Klonis, Nectarios, Ferguson, David J. P., Cowman, Alan F., Beck, Hans-Peter, ...

Malaria parasites export proteins beyond their own plasma membrane to locations in the red blood cells in which they reside. Maurer's clefts are parasite-derived structures within the host cell...

A Cluster of Ring Stage–specific Genes Linked to a Locus Implicated in Cytoadherence in Plasmodium falciparum Codes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell

Spielmann, Tobias, Hawthorne, Paula L., Dixon, Matthew W.A., Hannemann, Mandy, Klotz, Kathleen, Kemp, David J., ...

Blood stages of Plasmodium falciparum export proteins into their erythrocyte host, thereby inducing extensive host cell modifications that become apparent after the first half of the asexual...

Erythrocyte membrane vesiculation: Model for the molecular mechanism of protein sorting

Knowles, David W., Tilley, Leann, Mohandas, Narla, Chasis, Joel Anne

Budding and vesiculation of erythrocyte membranes occurs by a process involving an uncoupling of the membrane skeleton from the lipid bilayer. Vesicle formation provides an important means whereby...

Specific Inhibition of the Eubacterial DNA Ligase by Arylamino Compounds

Ciarrocchi, Giovanni, MacPhee, Donald G., Deady, Les W., Tilley, Leann

All known DNA ligases catalyze the formation of a phosphodiester linkage between adjacent termini in double-stranded DNA via very similar mechanisms. The ligase family can, however, be divided into...

Isolation of Peptides That Mimic Epitopes on a Malarial Antigen from Random Peptide Libraries Displayed on Phage

Adda, Christopher G., Tilley, Leann, Anders, Robin F., Foley, Michael

The ring-infected erythrocyte surface antigen (RESA) is a dense-granule protein of Plasmodium falciparum which binds to the cytoskeletal structure of the erythrocyte after parasite invasion. It is...

Trafficking and assembly of the cytoadherence complex in Plasmodium falciparum-infected human erythrocytes

Wickham, Mark E., Rug, Melanie, Ralph, Stuart A., Klonis, Nectarios, McFadden, Geoffrey I., Tilley, Leann, ...

After invading human erythrocytes, the malarial parasite Plasmodium falciparum, initiates a remarkable process of secreting proteins into the surrounding erythrocyte cytoplasm and plasma membrane....

Correct Promoter Control Is Needed for Trafficking of the Ring-Infected Erythrocyte Surface Antigen to the Host Cytosol in Transfected Malaria Parasites

Rug, Melanie, Wickham, Mark E., Foley, Michael, Cowman, Alan F., Tilley, Leann

Following invasion of human erythrocytes, the malaria parasite, Plasmodium falciparum, exports proteins beyond the confines of its own plasma membrane to modify the properties of the host red cell...

Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains

Frankland, Sarah, Adisa, Akinola, Horrocks, Paul, Taraschi, Theodore F., Schneider, Timothy, Elliott, Salenna R., ...

The particular virulence of the human malaria parasite Plasmodium falciparum derives from export of parasite-encoded proteins to the surface of the mature erythrocytes in which it resides. The...

Genesis of and Trafficking to the Maurer's Clefts of Plasmodium falciparum-Infected Erythrocytes†

Spycher, Cornelia, Rug, Melanie, Klonis, Nectarios, Ferguson, David J. P., Cowman, Alan F., Beck, Hans-Peter, ...

Malaria parasites export proteins beyond their own plasma membrane to locations in the red blood cells in which they reside. Maurer's clefts are parasite-derived structures within the host cell...

A Cluster of Ring Stage–specific Genes Linked to a Locus Implicated in Cytoadherence in Plasmodium falciparum Codes for PEXEL-negative and PEXEL-positive Proteins Exported into the Host Cell

Spielmann, Tobias, Hawthorne, Paula L., Dixon, Matthew W.A., Hannemann, Mandy, Klotz, Kathleen, Kemp, David J., ...

Blood stages of Plasmodium falciparum export proteins into their erythrocyte host, thereby inducing extensive host cell modifications that become apparent after the first half of the asexual...

Trafficking of the major virulence factor to the surface of transfected P falciparum–infected erythrocytes

Knuepfer, Ellen, Rug, Melanie, Klonis, Nectarios, Tilley, Leann, Cowman, Alan F.

After invading human red blood cells (RBCs) the malaria parasite Plasmodium falciparum remodels the host cell by trafficking proteins to the RBC compartment. The virulence protein P falciparum...

Selective permeabilization of the host cell membrane of Plasmodium falciparum-infected red blood cells with streptolysin O and equinatoxin II

Jackson, Katherine E., Spielmann, Tobias, Hanssen, Eric, Adisa, Akinola, Separovic, Frances, Dixon, Matthew W. A., ...

Plasmodium falciparum develops within the mature RBCs (red blood cells) of its human host in a PV (parasitophorous vacuole) that separates the host cell cytoplasm from the parasite surface. The...

Serum Lipoproteins Promote Efficient Presentation of the Malaria Virulence Protein PfEMP1 at the Erythrocyte Surface▿

Frankland, Sarah, Elliott, Salenna R., Yosaatmadja, Francisca, Beeson, James G., Rogerson, Stephen J., Adisa, Akinola, ...

The virulence of the malaria parasite Plasmodium falciparum is related to its ability to express a family of adhesive proteins known as P. falciparum erythrocyte membrane protein 1 (PfEMP1) at the...

Signal-mediated export of proteins from the malaria parasite to the host erythrocyte

Marti, Matthias, Baum, Jake, Rug, Melanie, Tilley, Leann, Cowman, Alan F.

Intracellular parasites from the genus Plasmodium reside and multiply in a variety of cells during their development. After invasion of human erythrocytes, asexual stages from the most virulent...

Characterization of the Antibody Response against Plasmodium falciparum Erythrocyte Membrane Protein 1 in Human Volunteers▿ †

Krause, Darren R., Gatton, Michelle L., Frankland, Sarah, Eisen, Damon P., Good, Michael F., Tilley, Leann, ...

The immune response against the Plasmodium falciparum variant surface antigen P. falciparum erythrocyte membrane protein 1 (PfEMP1) is a key component of clinical immunity against falciparum malaria....

Artemisinin and a Series of Novel Endoperoxide Antimalarials Exert Early Effects on Digestive Vacuole Morphology▿ ‡

Del Pilar Crespo, Maria, Avery, Thomas D., Hanssen, Eric, Fox, Emma, Robinson, Tony V., Valente, Peter, ...

Artermisinin and its derivatives are now the mainstays of antimalarial treatment; however, their mechanism of action is only poorly understood. We report on the synthesis of a novel series of...

Evaluation of pH during cytostomal endocytosis and vacuolar catabolism of haemoglobin in Plasmodium falciparum

Klonis, Nectarios, Tan, Olivia, Jackson, Katherine, Goldberg, Daniel, Klemba, Michael, Tilley, Leann

The DV (digestive vacuole) of the malaria parasite, Plasmodium falciparum, is the site of Hb (haemoglobin) digestion and haem detoxification and, as a consequence, the site of action of CQ...