EST and EST-SSR marker resources for Iris (2009)
Tang, Shunxue, Okashah, Rebecca A, Cordonnier-Pratt, Marie-Michele, Pratt, Lee H, Ed Johnson, Virgil, Taylor, Christopher A, ...
Abstract Background Limited DNA sequence and DNA marker resources have been developed for Iris (Iridaceae), a monocot genus of 200–300 species in the Asparagales, several of which are...
Lee H. Pratt, Chun Liang, Manish Shah, Feng Sun, Haiming Wang, St. Patrick Reid, ...
This article is published in Plant Physiology Online, Plant Physiology Preview Section, which publishes manuscripts accepted for publication after they have been edited and the authors have corrected...
Husaineid, Said S. H., Kok, Rosan A., Schreuder, Marielle E. L., Hanumappa, Mamatha, Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H., ...
Transgenic tomato [Lycopersicon esculentum (=Solanum lycopersicum)] lines overexpressing tomato PHYA, PHYB1, or PHYB2, under control of the constitutive double-35S promoter from cauliflower mosaic...
Husaineid, Said S. H., Kok, Rosan A., Schreuder, Marielle E. L., Hanumappa, Mamatha, Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H., ...
Transgenic tomato [Lycopersicon esculentum (=Solanum lycopersicum)] lines overexpressing tomato PHYA, PHYB1, or PHYB2, under control of the constitutive double-35S promoter from cauliflower mosaic...
MAGIC-SPP: a database-driven DNA sequence processing package with associated management tools (2006)
Liang, Chun, Sun, Feng, Wang, Haiming, Qu, Junfeng, Freeman, Robert M, Pratt, Lee H, ...
Abstract Background Processing raw DNA sequence data is an especially challenging task for relatively small laboratories and core facilities that produce as many as 5000 or more DNA sequences per...
MAGIC Database and Interfaces: An Integrated Package for Gene Discovery and Expression (2006)
Chun Liang, Haiming Wang, Dmitri S. Kolychev, Feng Sun, Robert Freeman, ...
The rapidly increasing rate at which biological data is being produced requires a corresponding growth in relational databases and associated tools that can help laboratories contend with that data....
MAGIC Database and Interfaces: An Integrated Package for Gene Discovery and Expression (2004)
Chun Liang, Haiming Wang, Dmitri S. Kolychev, Feng Sun, Robert Freeman, ...
The rapidly increasing rate at which biological data is being produced requires a corresponding growth in relational databases and associated tools that can help laboratories contend with that data....
Alba, Rob, Kelmenson, Peter M., Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H.
A reexamination of the genome of the tomato (renamed Solanum lycopersicum L.) indicates that it contains five, or at most perhaps six, phytochrome genes (PHY), each encoding a different apoprotein...
Isolation of the Red-light-absorbing Form of Phytochrome from Light-grown Pea Shoots (1981)
Shimazaki, Yukio, Moriyasu, Yuji, Pratt, Lee H., Furuya, Masaki
Phytochrome was extracted from both light-grown and dark-grown shoots of Pisum and partially purified by brushite chromatography and ammonium sulfate fractionation. About 160–270 ng of phytochrome...
A Photoperiod-Insensitive Barley Line Contains a Light-Labile Phytochrome B1
Hanumappa, Mamatha, Pratt, Lee H., Cordonnier-Pratt, Marie-Michele, Deitzer, Gerald F.
Barley (Hordeum vulgare L.) is a long-day plant whose flowering is enhanced when the photoperiod is supplemented with far-red light, and this promotion is mediated by phytochrome. A chemically...
Alba, Rob, Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H.
We show that phytochromes modulate differentially various facets of light-induced ripening of tomato fruit (Solanum lycopersicum L.). Northern analysis demonstrated that phytochrome A mRNA in fruit...
Gallagher, Sean, Short, Timothy W., Ray, Peter M., Pratt, Lee H., Briggs, Winslow R.
Irradiation of etiolated pea (Pisum sativum L.) seedlings with white light affects two proteins, both of monomer molecular mass near 120 kDa. Both proteins have been detected in association with...
Kidd, George H., Pratt, Lee H.
Examination of the phytochrome destruction reaction as a function of age in etiolated oat (Avena sativa L. cv. Garry) seedlings demonstrates that following illumination of 3-day-old shoots there is a...
Comparative Immunochemistry of Phytochrome
Partially purified high molecular weight preparations of phytochrome, estimated to be close to 440,000 molecular weight based upon chromatography through a calibrated Bio-Gel P-300 column, were...
Immunocytochemical Localization of Phytochrome
Pratt, Lee H., Coleman, Richard A.
An immunocytochemical assay, which localizes phytochrome in situ, indicates that this plant chromoprotein is associated with both nuclei and plastids, in addition to the cytoplasm. In an etiolated...
Boeshore, Maury L., Pratt, Lee H.
Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior...
Immunopurification and Initial Characterization of Dicotyledonous Phytochrome 12
Cordonnier, Marie-Michèle, Pratt, Lee H.
Antiserum was prepared against proteolytically undegraded phytochrome obtained from etiolated zucchini squash (Cucurbita pepo L., cv. Black Beauty). The antiserum was prepared by injecting into a...
Phytochrome Modification and Light-enhanced, In Vivo-induced Phytochrome Pelletability 12
Boeshore, Maury L., Pratt, Lee H.
Phytochrome that was induced by red irradiation in vivo to pellet with subcellular material and that was released from the pellet by removal of divalent cations exhibited altered characteristics....
Undegraded, highly purified [35S]phytochrome was immunoaffinity-purified either from dark control oat (cv. Garry) shoots or from etiolated oat shoots that were previously irradiated first with red...
Phytochrome Characterization by Rabbit Antiserum against High Molecular Weight Phytochrome 1
Cundiff, Susan C., Pratt, Lee H.
Both small and large sizes of phytochrome purified from Garry oat (Avena sativa L. ev. Garry) as well as large phytochrome purified from Newton oat (A. sativa L. cv. Newton), rye (Secale cereale L....
Immunological and Physical Characterization of the Products of Phytochrome Proteolysis 1
Cundiff, Susan C., Pratt, Lee H.
The relationship between high molecular weight (large) and low molecular weight (small) forms of phytochrome has been shown earlier to be one of proteolysis. The products of such proteolysis are...
Red Light-enhanced Phytochrome Pelletability: Re-examination and Further Characterization 1
Red light-enhanced pelletability of phytochrome was observed in extracts of all 11 plants tested: Avena sativa L., Secale cereale L., Zea mays L., Cucurbita pepo L., Sinapis alba L., Pisum sativum...
Characterization of the Destruction of Phytochrome in the Red-absorbing Form 1
Stone, Harry J., Pratt, Lee H.
Both the red-absorbing (Pr) and far red-absorbing (Pfr) forms of phytochrome undergo destruction, defined as the loss of photoreversibly detectable chromoprotein following actinic irradiation of...
Phytochrome Radioimmunoassay 1
Hunt, Robert E., Pratt, Lee H.
A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay...
Phytochrome Immunoaffinity Purification 1
Hunt, Robert E., Pratt, Lee H.
We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with...
Shimazaki, Yukio, Cordonnier, Marie-Michèle, Pratt, Lee H.
An enzyme-linked immunosorbent assay that revealed an antigenic difference between the red-absorbing and far-red-absorbing forms of phytochrome (Pr and Pfr, respectively) near its amino terminus...
Localization of Phytochrome in Oats by Electron Microscopy 1
Verbelen, Jean-Pierre, Pratt, Lee H., Butler, Warren L., Tokuyasu, Kiyoteru
Phytochrome was localized by immunoelectron microscopy in cells of the coleoptile tip of etiolated and irradiated oat (Avena sativa L., cv. Konata) seedlings. By using ultrathin frozen sections and...
Cordonnier, Marie-Michèle, Pratt, Lee H.
Preparation and characterization of antisera against lettuce (Lactuca sativa L., cv. Grand Rapids) and pea (Pisum sativum L., cv. Alaska) phytochrome is described. These antisera, together with...
Localization of Phytochrome in Oats by Electron Microscopy 1
Verbelen, Jean-Pierre, Pratt, Lee H., Butler, Warren L., Tokuyasu, Kiyoteru
Phytochrome was localized by immunoelectron microscopy in cells of the coleoptile tip of etiolated and irradiated oat (Avena sativa L., cv. Konata) seedlings. By using ultrathin frozen sections and...
Cordonnier, Marie-Michèle, Greppin, Hubert, Pratt, Lee H.
Nine monoclonal antibodies to pea (Pisum sativum L.) and 16 to oat (Avena sativa L.) phytochrome are characterized by enzyme-linked immunosorbent assay against phytochrome from six different sources:...
Identification of a Highly Conserved Domain on Phytochrome from Angiosperms to Algae 1
Cordonnier, Marie-Michèle, Greppin, Hubert, Pratt, Lee H.
A monoclonal antibody (Pea-25) directed to phytochrome from etiolated peas (Pisum sativum L., cv Alaska) binds to an antigenic domain that has been highly conserved throughout evolution. Antigenic...
Childs, Kevin L., Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H., Morgan, Page W.
Phytochrome content of three near-isogenic genotypes of Sorghum bicolor was analyzed using immunological and spectrophotometric means. Seedlings of the photoperiodically sensitive genotypes 90M...
Phytochrome Levels in Light-Grown Avena Change in Response to End-of-Day Irradiations 1
Stewart, Sandy J., Pratt, Lee H., Cordonnier-Pratt, [ill]-Michèle
The effect of 15-minute end-of-day irradiations on photoreversible phytochrome levels in light-grown oat (Avena sativa L., cv Garry) seedlings was investigated. Oat seedlings were grown in a cycle of...
Childs, Kevin L., Pratt, Lee H., Morgan, Page W.
Physiological processes controlled by phytochrome were examined in three near-isogenic genotypes of Sorghum bicolor, differing at the allele of the third maturity gene locus. Seedlings of 58M (ma3R...
Photochemical and Nonphotochemical Reactions of Phytochrome in vivo 1
Pratt, Lee H., Briggs, Winslow R.
The nonphotochemical reactions of phytochrome in the coleoptiles of dark-grown corn seedlings were studied at 3 temperatures: 14°, 24°, and 34°. The data obtained show that the destruction of Pfr...
Phytochrome Destruction: Apparent Inhibition by Ethylene 1
Stone, Harry J., Pratt, Lee H.
Phytochrome destruction begins immediately following actinic irradiation of 4-day-old, dark-grown oat (Avena sativa L., cv. Garry) shoots grown in open containers. When grown in closed containers,...
MAGIC-SPP: a database-driven DNA sequence processing package with associated management tools
Liang, Chun, Sun, Feng, Wang, Haiming, Qu, Junfeng, Freeman, Robert M, Pratt, Lee H, ...
Salzman, Ron A., Brady, Jeff A., Finlayson, Scott A., Buchanan, Christina D., Summer, Elizabeth J., Sun, Feng, ...
We have conducted a large-scale study of gene expression in the C4 monocot sorghum (Sorghum bicolor) L. Moench cv BTx623 in response to the signaling compounds salicylic acid (SA), methyl jasmonate...
Devos, Katrien M., Ma, Jianxin, Pontaroli, Ana C., Pratt, Lee H., Bennetzen, Jeffrey L.
The current view of wheat genome composition is that genes are compartmentalized into gene-rich and gene-poor regions. This model can be tested by analyzing randomly selected bacterial artificial...
Pratt, Lee H., Liang, Chun, Shah, Manish, Sun, Feng, Wang, Haiming, Reid, St. Patrick, ...
Improved knowledge of the sorghum transcriptome will enhance basic understanding of how plants respond to stresses and serve as a source of genes of value to agriculture. Toward this goal, Sorghum...
A Photoperiod-Insensitive Barley Line Contains a Light-Labile Phytochrome B1
Hanumappa, Mamatha, Pratt, Lee H., Cordonnier-Pratt, Marie-Michele, Deitzer, Gerald F.
Barley (Hordeum vulgare L.) is a long-day plant whose flowering is enhanced when the photoperiod is supplemented with far-red light, and this promotion is mediated by phytochrome. A chemically...
Alba, Rob, Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H.
We show that phytochromes modulate differentially various facets of light-induced ripening of tomato fruit (Solanum lycopersicum L.). Northern analysis demonstrated that phytochrome A mRNA in fruit...
Gallagher, Sean, Short, Timothy W., Ray, Peter M., Pratt, Lee H., Briggs, Winslow R.
Irradiation of etiolated pea (Pisum sativum L.) seedlings with white light affects two proteins, both of monomer molecular mass near 120 kDa. Both proteins have been detected in association with...
Kidd, George H., Pratt, Lee H.
Examination of the phytochrome destruction reaction as a function of age in etiolated oat (Avena sativa L. cv. Garry) seedlings demonstrates that following illumination of 3-day-old shoots there is a...
Comparative Immunochemistry of Phytochrome
Partially purified high molecular weight preparations of phytochrome, estimated to be close to 440,000 molecular weight based upon chromatography through a calibrated Bio-Gel P-300 column, were...
Immunocytochemical Localization of Phytochrome
Pratt, Lee H., Coleman, Richard A.
An immunocytochemical assay, which localizes phytochrome in situ, indicates that this plant chromoprotein is associated with both nuclei and plastids, in addition to the cytoplasm. In an etiolated...
Boeshore, Maury L., Pratt, Lee H.
Phytochrome that has been photoinduced to pellet by irradiation of intact oat (cv. Garry) shoots and recovered from a pellet obtained by centrifugation of crude extracts exhibits modified behavior...
Immunopurification and Initial Characterization of Dicotyledonous Phytochrome 12
Cordonnier, Marie-Michèle, Pratt, Lee H.
Antiserum was prepared against proteolytically undegraded phytochrome obtained from etiolated zucchini squash (Cucurbita pepo L., cv. Black Beauty). The antiserum was prepared by injecting into a...
Phytochrome Modification and Light-enhanced, In Vivo-induced Phytochrome Pelletability 12
Boeshore, Maury L., Pratt, Lee H.
Phytochrome that was induced by red irradiation in vivo to pellet with subcellular material and that was released from the pellet by removal of divalent cations exhibited altered characteristics....
Undegraded, highly purified [35S]phytochrome was immunoaffinity-purified either from dark control oat (cv. Garry) shoots or from etiolated oat shoots that were previously irradiated first with red...
Phytochrome Characterization by Rabbit Antiserum against High Molecular Weight Phytochrome 1
Cundiff, Susan C., Pratt, Lee H.
Both small and large sizes of phytochrome purified from Garry oat (Avena sativa L. ev. Garry) as well as large phytochrome purified from Newton oat (A. sativa L. cv. Newton), rye (Secale cereale L....
Immunological and Physical Characterization of the Products of Phytochrome Proteolysis 1
Cundiff, Susan C., Pratt, Lee H.
The relationship between high molecular weight (large) and low molecular weight (small) forms of phytochrome has been shown earlier to be one of proteolysis. The products of such proteolysis are...
Red Light-enhanced Phytochrome Pelletability: Re-examination and Further Characterization 1
Red light-enhanced pelletability of phytochrome was observed in extracts of all 11 plants tested: Avena sativa L., Secale cereale L., Zea mays L., Cucurbita pepo L., Sinapis alba L., Pisum sativum...
Characterization of the Destruction of Phytochrome in the Red-absorbing Form 1
Stone, Harry J., Pratt, Lee H.
Both the red-absorbing (Pr) and far red-absorbing (Pfr) forms of phytochrome undergo destruction, defined as the loss of photoreversibly detectable chromoprotein following actinic irradiation of...
Phytochrome Radioimmunoassay 1
Hunt, Robert E., Pratt, Lee H.
A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay...
Phytochrome Immunoaffinity Purification 1
Hunt, Robert E., Pratt, Lee H.
We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with...
Shimazaki, Yukio, Cordonnier, Marie-Michèle, Pratt, Lee H.
An enzyme-linked immunosorbent assay that revealed an antigenic difference between the red-absorbing and far-red-absorbing forms of phytochrome (Pr and Pfr, respectively) near its amino terminus...
Localization of Phytochrome in Oats by Electron Microscopy 1
Verbelen, Jean-Pierre, Pratt, Lee H., Butler, Warren L., Tokuyasu, Kiyoteru
Phytochrome was localized by immunoelectron microscopy in cells of the coleoptile tip of etiolated and irradiated oat (Avena sativa L., cv. Konata) seedlings. By using ultrathin frozen sections and...
Cordonnier, Marie-Michèle, Pratt, Lee H.
Preparation and characterization of antisera against lettuce (Lactuca sativa L., cv. Grand Rapids) and pea (Pisum sativum L., cv. Alaska) phytochrome is described. These antisera, together with...
Localization of Phytochrome in Oats by Electron Microscopy 1
Verbelen, Jean-Pierre, Pratt, Lee H., Butler, Warren L., Tokuyasu, Kiyoteru
Phytochrome was localized by immunoelectron microscopy in cells of the coleoptile tip of etiolated and irradiated oat (Avena sativa L., cv. Konata) seedlings. By using ultrathin frozen sections and...
Cordonnier, Marie-Michèle, Greppin, Hubert, Pratt, Lee H.
Nine monoclonal antibodies to pea (Pisum sativum L.) and 16 to oat (Avena sativa L.) phytochrome are characterized by enzyme-linked immunosorbent assay against phytochrome from six different sources:...
Identification of a Highly Conserved Domain on Phytochrome from Angiosperms to Algae 1
Cordonnier, Marie-Michèle, Greppin, Hubert, Pratt, Lee H.
A monoclonal antibody (Pea-25) directed to phytochrome from etiolated peas (Pisum sativum L., cv Alaska) binds to an antigenic domain that has been highly conserved throughout evolution. Antigenic...
Childs, Kevin L., Cordonnier-Pratt, Marie-Michèle, Pratt, Lee H., Morgan, Page W.
Phytochrome content of three near-isogenic genotypes of Sorghum bicolor was analyzed using immunological and spectrophotometric means. Seedlings of the photoperiodically sensitive genotypes 90M...
Phytochrome Levels in Light-Grown Avena Change in Response to End-of-Day Irradiations 1
Stewart, Sandy J., Pratt, Lee H., Cordonnier-Pratt, [ill]-Michèle
The effect of 15-minute end-of-day irradiations on photoreversible phytochrome levels in light-grown oat (Avena sativa L., cv Garry) seedlings was investigated. Oat seedlings were grown in a cycle of...
Childs, Kevin L., Pratt, Lee H., Morgan, Page W.
Physiological processes controlled by phytochrome were examined in three near-isogenic genotypes of Sorghum bicolor, differing at the allele of the third maturity gene locus. Seedlings of 58M (ma3R...
Photochemical and Nonphotochemical Reactions of Phytochrome in vivo 1
Pratt, Lee H., Briggs, Winslow R.
The nonphotochemical reactions of phytochrome in the coleoptiles of dark-grown corn seedlings were studied at 3 temperatures: 14°, 24°, and 34°. The data obtained show that the destruction of Pfr...
Phytochrome Destruction: Apparent Inhibition by Ethylene 1
Stone, Harry J., Pratt, Lee H.
Phytochrome destruction begins immediately following actinic irradiation of 4-day-old, dark-grown oat (Avena sativa L., cv. Garry) shoots grown in open containers. When grown in closed containers,...
Salzman, Ron A., Brady, Jeff A., Finlayson, Scott A., Buchanan, Christina D., Summer, Elizabeth J., Sun, Feng, ...
We have conducted a large-scale study of gene expression in the C4 monocot sorghum (Sorghum bicolor) L. Moench cv BTx623 in response to the signaling compounds salicylic acid (SA), methyl jasmonate...
Pratt, Lee H., Liang, Chun, Shah, Manish, Sun, Feng, Wang, Haiming, Reid, St. Patrick, ...
Improved knowledge of the sorghum transcriptome will enhance basic understanding of how plants respond to stresses and serve as a source of genes of value to agriculture. Toward this goal, Sorghum...
Devos, Katrien M., Ma, Jianxin, Pontaroli, Ana C., Pratt, Lee H., Bennetzen, Jeffrey L.
The current view of wheat genome composition is that genes are compartmentalized into gene-rich and gene-poor regions. This model can be tested by analyzing randomly selected bacterial artificial...
MAGIC-SPP: a database-driven DNA sequence processing package with associated management tools
Liang, Chun, Sun, Feng, Wang, Haiming, Qu, Junfeng, Freeman, Robert M, Pratt, Lee H, ...
MAGIC Database and Interfaces: An Integrated Package for Gene Discovery and Expression
Cordonnier-Pratt, Marie-Michèle, Liang, Chun, Wang, Haiming, Kolychev, Dmitri S., Sun, Feng, Freeman, Robert, ...
The rapidly increasing rate at which biological data is being produced requires a corresponding growth in relational databases and associated tools that can help laboratories contend with that data....
Conner, Joann A., Goel, Shailendra, Gunawan, Gunawati, Cordonnier-Pratt, Marie-Michele, Johnson, Virgil Ed, Liang, Chun, ...
Apomixis, asexual reproduction through seed, is widespread among angiosperm families. Gametophytic apomixis in Pennisetum squamulatum and Cenchrus ciliaris is controlled by the apospory-specific...