Helicobacter pylori Cytotoxin-Associated Genotype and Gastric Precancerous Lesions (2007)
Plummer, Martyn, Van Doorn, Leen-Jan, Franceschi, Silvia, Kleter, Bernhard, Canzian, Federico, Vivas, Jorge, ...
Background Helicobacter pylori infection is associated with the development of gastric cancer. Although infection with an H. pylori strain containing the cytotoxin-associated (cag A) gene (a marker...
Figueiredo, Céu, Machado, José Carlos, Pharoah, Paul, Seruca, Raquel, Sousa, Sónia, Carvalho, Ralph, ...
Background: Both Helicobacter pylori genotype and host genetic polymorphisms play a role in determining the clinical consequences of H. pylori infection. We investigated whether there are any...
Human Papillomavirus and the Long-term Risk of Cervical Neoplasia (2002)
Van Der Graaf, Yolanda, Molijn, Anco, Doornewaard, Heleen, Quint, Wim, Van Doorn, Leen-Jan, Van Den Tweel, Jan
The risk of cervical neoplasia for women with normal Papanicolaou smears was calculated for those whose smears were human papillomavirus (HPV) positive and those whose smears were HPV negative. Data...
Van Doorn, Leen-Jan, Burnens, André, Huysmans, Michael, Vandamme, Peter, Giesendorf, Belinda A. J., ...
Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow...
Distinct Variants of Helicobacter pylori cagA Are Associated with vacA Subtypes
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Blaser, Martin J., Quint, Wim G. V.
The diversity of the cytotoxin-associated gene (cagA) of Helicobacter pylori was analyzed in 45 isolates obtained from nine countries. We examined variation in the 5′ end of the cagA open reading...
Namavar, Ferry, Van Doorn, Leen-Jan, Durrani, Zarmina, Kuipers, Ernst J.
The vacA s and m genotypes and the presence of cagA and IS605 were determined in Helicobacter pylori strains from patients with mono- and multiple infections. Surprisingly, these genetic markers were...
Kleter, Bernhard, Van Doorn, Leen-Jan, Schrauwen, Lianne, Molijn, Anco, Sastrowijoto, Suprapto, Ter Schegget, Jan, ...
Human papillomavirus (HPV) can be detected by amplification of viral DNA. A novel PCR primer set generating a short PCR fragment (SPF PCR) was used for amplification of a fragment of only 65 bp from...
Van Doorn, Leen-Jan, Henskens, Yvonne, Nouhan, Nathalie, Verschuuren, Anita, Vreede, Rolf, Herbink, Paul, ...
A total of 500 consecutive patients undergoing upper endoscopy were biopsied and tested for H. pylori infection by the Campylobacter-like organism (CLO) test, culture, histology, and PCR. Serum...
Expanding Allelic Diversity of Helicobacter pylori vacA
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Pena, Salvador, Midolo, Peter, Ng, Enders K. W., ...
Distinct Variants of Helicobacter pylori cagA Are Associated with vacA Subtypes
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Blaser, Martin J., Quint, Wim G. V.
Characteristics of Helicobacter pylori Infection in Jamaican Adults with Gastrointestinal Symptoms
Hisada, Michie, Lee, Michael G., Hanchard, Barrie, Owens, Marilyn, Song, Qunsheng, Van Doorn, Leen-Jan, ...
Helicobacter pylori infection is common in Jamaica. Describing its epidemiology in a population-based study depends largely on serology, but serologic assays have not been validated in this...
De Groote, Dominic, Haesebrouck, Freddy, Van Doorn, Leen-Jan, Vandamme, Peter, Ducatelle, Richard
A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter...
Assessment of Helicobacter pylori vacA and cagA Genotypes and Host Serological Response
Figueiredo, Céu, Quint, Wim, Nouhan, Nathalie, Van Den Munckhof, Henk, Herbrink, Paul, Scherpenisse, Joost, ...
Helicobacter pylori strains can be distinguished by genotyping of virulence-associated genes, such as vacA and cagA. Because serological discrimination between strain types would reduce the need for...
Genotypic, Clinical, and Demographic Characteristics of Children Infected with Helicobacter pylori
Gold, Benjamin D., Van Doorn, Leen-Jan, Guarner, Jeannette, Owens, Marilyn, Pierce-Smith, Daphne, Song, Qunsheng, ...
Helicobacter pylori isolates vary between geographic regions. Certain H. pylori genotypes may be associated with disease outcome. Thirty-eight children underwent diagnostic upper endoscopy at four...
Ryan, Kieran A., Van Doorn, Leen-Jan, Moran, Anthony P., Glennon, Maura, Smith, Terry, Maher, Majella
The prevalence of clarithromycin resistance-associated mutations, the cytotoxin-associated gene (cagA), and the various vacuolating cytotoxin (vacA) genotypes was determined in 50 gastric biopsy...
Van Doorn, Leen-Jan, Debets-Ossenkopp, Yvette J., Marais, Armelle, Sanna, Ricardo, Mégraud, Francis, Kusters, Johannes G., ...
A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations,...
Van Doorn, Leen-Jan, Glupczynski, Youri, Kusters, Johannes G., Mégraud, Francis, Midolo, Peter, Maggi-Solcà, Nadia, ...
Helicobacter pylori strains from 299 patients were tested in six laboratories in different countries. Macrolide susceptibility of the strains was determined by agar dilution (17.4%) or the...
Helicobacter pylori-Associated Gastritis in Mice is Host and Strain Specific
Namavar, Ferry, Sparrius, Marion, Stoof, Jeroen, Van Rees, Emmelien P., Van Doorn, Leen-Jan, ...
The vacA and cagA geno- and phenotypes of two mouse-adapted strains of Helicobacter pylori, SS1 and SPM326, were determined. The SS1 strain, which had the cagA+ and vacA s2-m2 genotype, induced...
Peek, Richard M., Van Doorn, Leen-Jan, Donahue, John P., Tham, Kyi T., Figueiredo, Ceu, Blaser, Martin J., ...
The iceA locus of Helicobacter pylori includes one of two mutually exclusive gene families, iceA1 and iceA2. Colonization with iceA1 strains is associated with enhanced acute mucosal inflammation,...
Schneeberger, Peter M., Keur, Ingrid, Van Der Vliet, Walter, Van Hoek, Kitty, Boswijk, Henk, Van Loon, Anton M., ...
A national survey of hepatitis C virus (HCV) infections among dialysis patients in The Netherlands was performed. The study involved 2,653 patients (2,108 hemodialysis patients and 545 chronic...
Expanding Allelic Diversity of Helicobacter pylori vacA
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Pena, Salvador, Midolo, Peter, Ng, Enders K. W., ...
The diversity of the gene encoding the vacuolating cytotoxin (vacA) of Helicobacter pylori was analyzed in 98 isolates obtained from different geographic locations. The studies focused on variation...
Van Doorn, Leen-Jan, Quint, Wim, Kleter, Bernhard, Molijn, Anco, Colau, Brigitte, Martin, Marie-Thérèse, ...
A comparison of two PCR-based human papillomavirus (HPV) DNA detection and genotyping systems (PGMY LBA and SPF10 LiPA) was conducted in two laboratories. Both systems are based on broad-spectrum PCR...
Levi, José E., Kleter, Bernhard, Quint, Wim G. V., Fink, Maria C. S., Canto, Cynthia L. M., Matsubara, Regina, ...
A group of 208 human immunodeficiency virus (HIV)-infected women in Brazil were studied for the presence of human papillomavirus with the general SPF10 PCR primer set. Virtually all (98%) women were...
Regional Variation among vacA Alleles of Helicobacter pylori in China
Wang, Jingtong, Van Doorn, Leen-Jan, Robinson, Philip A., Ji, Xuhuai, Wang, Dong, Wang, Yu, ...
Allelic variation among Helicobacter pylori vacA occurs in the signal and middle region of the gene. The aim of the study was to investigate alleleic variation of vacA among H. pylori strains from...
Regional Variation among vacA Alleles of Helicobacter pylori in China
Wang, Jingtong, Van Doorn, Leen-Jan, Robinson, Philip A., Ji, Xuhuai, Wang, Dong, Wang, Yu, ...
Smits, Paul H. M., Bakker, Remco, Jong, Eefje, Mulder, Jan Willem, Meenhorst, Pieter L., Kleter, Bernhard, ...
Infection with human immunodeficiency virus (HIV) and the resulting immunosuppression are associated with an increased risk for human papillomavirus (HPV) persistence and related malignancies. In the...
De Koning, Maurits, Quint, Wim, Struijk, Linda, Kleter, Bernhard, Wanningen, Patrick, Van Doorn, Leen-Jan, ...
Human papillomavirus can be detected by amplification of viral DNA. A novel one-step PCR (PM-PCR) was evaluated for amplification of a 117-bp fragment from the E1 region. It permitted ultrasensitive...
Van Doorn, Leen-Jan, Burnens, André, Huysmans, Michael, Vandamme, Peter, Giesendorf, Belinda A. J., ...
Recently, a gene from Campylobacter jejuni encoding a putative GTPase was identified. Based on two semiconserved GTP-binding sites encoded within this gene, PCR primers were selected that allow...
Distinct Variants of Helicobacter pylori cagA Are Associated with vacA Subtypes
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Blaser, Martin J., Quint, Wim G. V.
The diversity of the cytotoxin-associated gene (cagA) of Helicobacter pylori was analyzed in 45 isolates obtained from nine countries. We examined variation in the 5′ end of the cagA open reading...
Namavar, Ferry, Van Doorn, Leen-Jan, Durrani, Zarmina, Kuipers, Ernst J.
The vacA s and m genotypes and the presence of cagA and IS605 were determined in Helicobacter pylori strains from patients with mono- and multiple infections. Surprisingly, these genetic markers were...
Kleter, Bernhard, Van Doorn, Leen-Jan, Schrauwen, Lianne, Molijn, Anco, Sastrowijoto, Suprapto, Ter Schegget, Jan, ...
Human papillomavirus (HPV) can be detected by amplification of viral DNA. A novel PCR primer set generating a short PCR fragment (SPF PCR) was used for amplification of a fragment of only 65 bp from...
Van Doorn, Leen-Jan, Henskens, Yvonne, Nouhan, Nathalie, Verschuuren, Anita, Vreede, Rolf, Herbink, Paul, ...
A total of 500 consecutive patients undergoing upper endoscopy were biopsied and tested for H. pylori infection by the Campylobacter-like organism (CLO) test, culture, histology, and PCR. Serum...
Expanding Allelic Diversity of Helicobacter pylori vacA
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Pena, Salvador, Midolo, Peter, Ng, Enders K. W., ...
Distinct Variants of Helicobacter pylori cagA Are Associated with vacA Subtypes
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Blaser, Martin J., Quint, Wim G. V.
Characteristics of Helicobacter pylori Infection in Jamaican Adults with Gastrointestinal Symptoms
Hisada, Michie, Lee, Michael G., Hanchard, Barrie, Owens, Marilyn, Song, Qunsheng, Van Doorn, Leen-Jan, ...
Helicobacter pylori infection is common in Jamaica. Describing its epidemiology in a population-based study depends largely on serology, but serologic assays have not been validated in this...
De Groote, Dominic, Haesebrouck, Freddy, Van Doorn, Leen-Jan, Vandamme, Peter, Ducatelle, Richard
A new specific and sensitive 16S ribosomal DNA-based PCR assay was developed. The assay targets a 78-bp DNA fragment unique to Helicobacter bizzozeronii, Helicobacter felis, and Helicobacter...
Assessment of Helicobacter pylori vacA and cagA Genotypes and Host Serological Response
Figueiredo, Céu, Quint, Wim, Nouhan, Nathalie, Van Den Munckhof, Henk, Herbrink, Paul, Scherpenisse, Joost, ...
Helicobacter pylori strains can be distinguished by genotyping of virulence-associated genes, such as vacA and cagA. Because serological discrimination between strain types would reduce the need for...
Genotypic, Clinical, and Demographic Characteristics of Children Infected with Helicobacter pylori
Gold, Benjamin D., Van Doorn, Leen-Jan, Guarner, Jeannette, Owens, Marilyn, Pierce-Smith, Daphne, Song, Qunsheng, ...
Helicobacter pylori isolates vary between geographic regions. Certain H. pylori genotypes may be associated with disease outcome. Thirty-eight children underwent diagnostic upper endoscopy at four...
Ryan, Kieran A., Van Doorn, Leen-Jan, Moran, Anthony P., Glennon, Maura, Smith, Terry, Maher, Majella
The prevalence of clarithromycin resistance-associated mutations, the cytotoxin-associated gene (cagA), and the various vacuolating cytotoxin (vacA) genotypes was determined in 50 gastric biopsy...
Van Doorn, Leen-Jan, Debets-Ossenkopp, Yvette J., Marais, Armelle, Sanna, Ricardo, Mégraud, Francis, Kusters, Johannes G., ...
A PCR-based reverse hybridization system (research prototype kit INNO-LiPA for H. pylori resistance) was developed and evaluated for simultaneous detection of 23S ribosomal DNA point mutations,...
Van Doorn, Leen-Jan, Glupczynski, Youri, Kusters, Johannes G., Mégraud, Francis, Midolo, Peter, Maggi-Solcà, Nadia, ...
Helicobacter pylori strains from 299 patients were tested in six laboratories in different countries. Macrolide susceptibility of the strains was determined by agar dilution (17.4%) or the...
Helicobacter pylori-Associated Gastritis in Mice is Host and Strain Specific
Namavar, Ferry, Sparrius, Marion, Stoof, Jeroen, Van Rees, Emmelien P., Van Doorn, Leen-Jan, ...
The vacA and cagA geno- and phenotypes of two mouse-adapted strains of Helicobacter pylori, SS1 and SPM326, were determined. The SS1 strain, which had the cagA+ and vacA s2-m2 genotype, induced...
Peek, Richard M., Van Doorn, Leen-Jan, Donahue, John P., Tham, Kyi T., Figueiredo, Ceu, Blaser, Martin J., ...
The iceA locus of Helicobacter pylori includes one of two mutually exclusive gene families, iceA1 and iceA2. Colonization with iceA1 strains is associated with enhanced acute mucosal inflammation,...
Schneeberger, Peter M., Keur, Ingrid, Van Der Vliet, Walter, Van Hoek, Kitty, Boswijk, Henk, Van Loon, Anton M., ...
A national survey of hepatitis C virus (HCV) infections among dialysis patients in The Netherlands was performed. The study involved 2,653 patients (2,108 hemodialysis patients and 545 chronic...
Expanding Allelic Diversity of Helicobacter pylori vacA
Van Doorn, Leen-Jan, Figueiredo, Céu, Sanna, Ricardo, Pena, Salvador, Midolo, Peter, Ng, Enders K. W., ...
The diversity of the gene encoding the vacuolating cytotoxin (vacA) of Helicobacter pylori was analyzed in 98 isolates obtained from different geographic locations. The studies focused on variation...
Van Doorn, Leen-Jan, Quint, Wim, Kleter, Bernhard, Molijn, Anco, Colau, Brigitte, Martin, Marie-Thérèse, ...
A comparison of two PCR-based human papillomavirus (HPV) DNA detection and genotyping systems (PGMY LBA and SPF10 LiPA) was conducted in two laboratories. Both systems are based on broad-spectrum PCR...
Levi, José E., Kleter, Bernhard, Quint, Wim G. V., Fink, Maria C. S., Canto, Cynthia L. M., Matsubara, Regina, ...
A group of 208 human immunodeficiency virus (HIV)-infected women in Brazil were studied for the presence of human papillomavirus with the general SPF10 PCR primer set. Virtually all (98%) women were...
Regional Variation among vacA Alleles of Helicobacter pylori in China
Wang, Jingtong, Van Doorn, Leen-Jan, Robinson, Philip A., Ji, Xuhuai, Wang, Dong, Wang, Yu, ...
Allelic variation among Helicobacter pylori vacA occurs in the signal and middle region of the gene. The aim of the study was to investigate alleleic variation of vacA among H. pylori strains from...
Regional Variation among vacA Alleles of Helicobacter pylori in China
Wang, Jingtong, Van Doorn, Leen-Jan, Robinson, Philip A., Ji, Xuhuai, Wang, Dong, Wang, Yu, ...
Smits, Paul H. M., Bakker, Remco, Jong, Eefje, Mulder, Jan Willem, Meenhorst, Pieter L., Kleter, Bernhard, ...
Infection with human immunodeficiency virus (HIV) and the resulting immunosuppression are associated with an increased risk for human papillomavirus (HPV) persistence and related malignancies. In the...
De Koning, Maurits, Quint, Wim, Struijk, Linda, Kleter, Bernhard, Wanningen, Patrick, Van Doorn, Leen-Jan, ...
Human papillomavirus can be detected by amplification of viral DNA. A novel one-step PCR (PM-PCR) was evaluated for amplification of a 117-bp fragment from the E1 region. It permitted ultrasensitive...
Van Doorn, Leen-Jan, Molijn, Anco, Kleter, Bernhard, Quint, Wim, Colau, Brigitte
The use of a single broad-spectrum human papillomavirus (HPV) DNA-based PCR test may fail to detect lower concentrations of HPV DNA due to competition between different genotypes in mixed infections....
Kleter, Bernhard, Van Doorn, Leen-Jan, Ter Schegget, Jan, Schrauwen, Lianne, Van Krimpen, Kees, Burger, Matthé, ...
A novel set of polymerase chain reaction (PCR) primers, designated SPF1 and SPF2 and located in the L1 region, was developed for universal detection of human papillomavirus (HPV). A short PCR...
Helicobacter pylori Genotypes May Determine Gastric Histopathology
Nogueira, Cristina, Figueiredo, Céu, Carneiro, Fátima, Taveira Gomes, António, Barreira, Raul, Figueira, Paulo, ...
The outcome of Helicobacter pylori infection has been associated with specific virulence-associated bacterial genotypes. The present study aimed to investigate the gastric histopathology in...
Safaeian, Mahboobeh, Herrero, Rolando, Hildesheim, Allan, Quint, Wim, Freer, Enrique, Van Doorn, Leen-Jan, ...
The objective of this analysis was to compare the performance characteristics of two human papillomavirus (HPV) DNA detections assays, the Hybrid Capture 2 assay (HC2) and the SPF10 assay, for the...
Quint, Koen, Porras, Carolina, Safaeian, Mahboobeh, González, Paula, Hildesheim, Allan, Quint, Wim, ...
The aims of this study were to compare a novel PCR-based Chlamydia trachomatis detection and genotyping (Ct-DT) assay with the FDA-approved, commercially available C. trachomatis detection Hybrid...
Quint, Koen D., Van Doorn, Leen-Jan, Kleter, Bernhard, Morre, Servaas A., ...
Chlamydia trachomatis (Ct) comprises distinct serogroups and serovars. The present study evaluates a novel Ct amplification, detection, and genotyping method (Ct-DT assay). The Ct-DT amplification...
Comparison of Two PCR-Based Human Papillomavirus Genotyping Methods▿ †
Castle, Philip E., Porras, Carolina, Quint, Wim G., Rodriguez, Ana Cecilia, Schiffman, Mark, Gravitt, Patti E., ...
We compared two consensus primer PCR human papillomavirus (HPV) genotyping methods for the detection of individual HPV genotypes and carcinogenic HPV genotypes as a group, using a stratified sample...