Leon D. Kluskens

Influence of Shifting Positions of Ser, Thr, and Cys Residues in Prenisin on the Efficiency of Modification Reactions and on the Antimicrobial Activities of the Modified Prepeptides (2008)

Lubelski, Jacek, Overkamp, Wout, Kluskens, Leon D., Moll, Gert N., Kuipers, Oscar P.

Since the recent discovery that the nisin modification and transport machinery can be used to produce and modify peptides unrelated to nisin, specific questions arose concerning the specificity of...

Production of Dehydroamino Acid-Containing Peptides by Lactococcus lactis (2007)

Rink, Rick, Wierenga, Jenny, Kuipers, Anneke, Kluskens, Leon D., Driessen, Arnold J.M., Kuipers, Oscar P., ...

Nisin is a pentacyclic peptide antibiotic produced by some Lactococcus lactis strains. Nisin contains dehydroresidues and thioether rings that are posttranslationally introduced by a...

Dissection and Modulation of the Four Distinct Activities of Nisin by Mutagenesis of Rings A and B and by C-Terminal Truncation (2007)

Rink, Rick, Wierenga, Jenny, Kuipers, Anneke, Kluskens, Leon D., Driessen, Arnold J.M., Kuipers, Oscar P., ...

Nisin A is a pentacyclic antibiotic peptide produced by various Lactococcus lactis strains. Nisin displays four different activities: (i) it autoinduces its own synthesis; (ii) it inhibits the growth...

NisC, the Cyclase of the Lantibiotic Nisin, Can Catalyze Cyclization of Designed Nonlantibiotic Peptides (2007)

Rink, Rick, Kluskens, Leon D., Kuipers, Anneke, Driessen, Arnold J.M., Kuipers, Oscar P., Moll, Gert N.

Nisin is a pentacyclic peptide antibiotic active against Gram-positive bacteria. Its thioether rings are formed by two enzymatic steps: nisin dehydratase (NisB)-mediated dehydration of serines and...

Sec-Mediated Transport of Posttranslationally Dehydrated Peptides in Lactococcus lactis (2006)

Kuipers, Anneke, Wierenga, Jenny, Rink, Rick, Kluskens, Leon D., Driessen, Arnold J.M., Kuipers, Oscar P., ...

Nisin is a lanthionine-containing antimicrobial peptide produced by Lactococcus lactis. Its (methyl)lanthionines are introduced by two posttranslational enzymatic steps involving the dehydratase...

Post-translational Modification of Therapeutic Peptides By NisB, the Dehydratase of the Lantibiotic Nisin (2005)

Kluskens, Leon D., Kuipers, Anneke, Rink, Rick, Boef, Esther De, Fekken, Susan, Driessen, Arnold J.M., ...

Post-translationally introduced dehydroamino acids often play an important role in the activity and receptor specificity of biologically active peptides. In addition, a dehydroamino acid can be...

NisT, the Transporter of the Lantibiotic Nisin, Can Transport Fully Modified, Dehydrated, and Unmodified Prenisin and Fusions of the Leader Peptide with Non-lantibiotic Peptides (2004)

Kuipers, Anneke, Boef, Esther De, Rink, Rick, Fekken, Susan, Kluskens, Leon D., Driessen, Arnold J.M., ...

Lantibiotics are lanthionine-containing peptide antibiotics. Nisin, encoded by nisA, is a pentacyclic lantibiotic produced by some Lactococcus lactis strains. Its thioether rings are...

Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima.

Kluskens, Leon D, Voragen, Alphons G J, De Vos, Willem M, Van Der Oost, John

The ability of the hyperthermophilic bacterium Thermotoga maritima to grow on pectin as a sole carbon source coincides with the secretion of a pectate lyase A (PelA) in the extracellular medium. The...

Cloning and Expression of Islandisin, a New Thermostable Subtilisin from Fervidobacterium islandicum, in Escherichia coli

Gödde, Carolin, Sahm, Kerstin, Brouns, Stan J. J., Kluskens, Leon D., Van Der Oost, John, De Vos, Willem M., ...

A gene encoding a subtilisin-like protease, designated islandisin, from the extremely thermophilic bacterium Fervidobacterium islandicum (DSMZ 5733) was cloned and actively expressed in Escherichia...

Cloning and Expression of Islandisin, a New Thermostable Subtilisin from Fervidobacterium islandicum, in Escherichia coli

Gödde, Carolin, Sahm, Kerstin, Brouns, Stan J. J., Kluskens, Leon D., Van Der Oost, John, De Vos, Willem M., ...

A gene encoding a subtilisin-like protease, designated islandisin, from the extremely thermophilic bacterium Fervidobacterium islandicum (DSMZ 5733) was cloned and actively expressed in Escherichia...

Molecular and biochemical characterization of the thermoactive family 1 pectate lyase from the hyperthermophilic bacterium Thermotoga maritima.

Kluskens, Leon D, Voragen, Alphons G J, De Vos, Willem M, Van Der Oost, John

The ability of the hyperthermophilic bacterium Thermotoga maritima to grow on pectin as a sole carbon source coincides with the secretion of a pectate lyase A (PelA) in the extracellular medium. The...

Sec-Mediated Transport of Posttranslationally Dehydrated Peptides in Lactococcus lactis▿ †

Kuipers, Anneke, Wierenga, Jenny, Rink, Rick, Kluskens, Leon D., Driessen, Arnold J. M., Kuipers, Oscar P., ...

Nisin is a lanthionine-containing antimicrobial peptide produced by Lactococcus lactis. Its (methyl)lanthionines are introduced by two posttranslational enzymatic steps involving the dehydratase...

Production of Dehydroamino Acid-Containing Peptides by Lactococcus lactis▿

Rink, Rick, Wierenga, Jenny, Kuipers, Anneke, Kluskens, Leon D., Driessen, Arnold J. M., Kuipers, Oscar P., ...

Nisin is a pentacyclic peptide antibiotic produced by some Lactococcus lactis strains. Nisin contains dehydroresidues and thioether rings that are posttranslationally introduced by a...

Dissection and Modulation of the Four Distinct Activities of Nisin by Mutagenesis of Rings A and B and by C-Terminal Truncation▿ †

Rink, Rick, Wierenga, Jenny, Kuipers, Anneke, Kluskens, Leon D., Driessen, Arnold J. M., Kuipers, Oscar P., ...

Nisin A is a pentacyclic antibiotic peptide produced by various Lactococcus lactis strains. Nisin displays four different activities: (i) it autoinduces its own synthesis; (ii) it inhibits the growth...

Influence of Shifting Positions of Ser, Thr, and Cys Residues in Prenisin on the Efficiency of Modification Reactions and on the Antimicrobial Activities of the Modified Prepeptides▿ †

Lubelski, Jacek, Overkamp, Wout, Kluskens, Leon D., Moll, Gert N., Kuipers, Oscar P.

Since the recent discovery that the nisin modification and transport machinery can be used to produce and modify peptides unrelated to nisin, specific questions arose concerning the specificity of...