Shaw, Michael K., Tilney, Lewis G.
The invasive stages of Toxoplasma gondii, an Apicomplexan parasite, actively invade their host cells in an actin-dependent way. However, despite containing biochemically significant amounts of actin,...
Role of Listeriolysin O in Cell-to-Cell Spread of Listeria monocytogenes
Gedde, Margaret M., Higgins, Darren E., Tilney, Lewis G., Portnoy, Daniel A.
Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a host vacuolar compartment and grows rapidly in the cytosol. Listeriolysin O (LLO) is a secreted...
A plastid segregation defect in the protozoan parasite Toxoplasma gondii
He, Cynthia Y., Shaw, Michael K., Pletcher, Charles H., Striepen, Boris, Tilney, Lewis G., Roos, David S.
Apicomplexan parasites—including the causative agents of malaria (Plasmodium sp.) and toxoplasmosis (Toxoplasma gondii)—harbor a secondary endosymbiotic plastid, acquired by lateral genetic...
Tilney, Lewis G., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Guild, Gregory M.
Drosophila bristle cells are shaped during growth by longitudinal bundles of cross-linked actin filaments attached to the plasma membrane. We used confocal and electron microscopy to examine actin...
Actin in the Brush-Border of Epithelial Cells of the Chicken Intestine
Tilney, Lewis G., Mooseker, Mark
The major soluble protein of the isolated brush-border of the intestinal epithelium has a molecular weight and net charge indistinguishable from those of skeletal-muscle actin, as determined by...
The Role Actin Filaments Play in Providing the Characteristic Curved Form of Drosophila BristlesD⃞
Tilney, Lewis G., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Guild, Gregory M., DeRosier, David
Drosophila bristles display a precise orientation and curvature. An asymmetric extension of the socket cell overlies the newly emerging bristle rudiment to provide direction for bristle elongation, a...
Guild, Gregory M., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Tilney, Lewis G.
Actin filament bundles can shape cellular extensions into dramatically different forms. We examined cytoskeleton formation during wing hair morphogenesis using both confocal and electron microscopy....
How to make a curved Drosophila bristle using straight actin bundles
Tilney, Lewis G., DeRosier, David J.
This, our Inaugural Article as Academy Members, is ironically our swan song from the field of the actin cytoskeleton. By reviewing what we have learned and what we think is going on during...
Shaw, Michael K., Tilney, Lewis G.
The invasive stages of Toxoplasma gondii, an Apicomplexan parasite, actively invade their host cells in an actin-dependent way. However, despite containing biochemically significant amounts of actin,...
Role of Listeriolysin O in Cell-to-Cell Spread of Listeria monocytogenes
Gedde, Margaret M., Higgins, Darren E., Tilney, Lewis G., Portnoy, Daniel A.
Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from a host vacuolar compartment and grows rapidly in the cytosol. Listeriolysin O (LLO) is a secreted...
A plastid segregation defect in the protozoan parasite Toxoplasma gondii
He, Cynthia Y., Shaw, Michael K., Pletcher, Charles H., Striepen, Boris, Tilney, Lewis G., Roos, David S.
Apicomplexan parasites—including the causative agents of malaria (Plasmodium sp.) and toxoplasmosis (Toxoplasma gondii)—harbor a secondary endosymbiotic plastid, acquired by lateral genetic...
Tilney, Lewis G., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Guild, Gregory M.
Drosophila bristle cells are shaped during growth by longitudinal bundles of cross-linked actin filaments attached to the plasma membrane. We used confocal and electron microscopy to examine actin...
Actin in the Brush-Border of Epithelial Cells of the Chicken Intestine
Tilney, Lewis G., Mooseker, Mark
The major soluble protein of the isolated brush-border of the intestinal epithelium has a molecular weight and net charge indistinguishable from those of skeletal-muscle actin, as determined by...
The Role Actin Filaments Play in Providing the Characteristic Curved Form of Drosophila BristlesD⃞
Tilney, Lewis G., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Guild, Gregory M., DeRosier, David
Drosophila bristles display a precise orientation and curvature. An asymmetric extension of the socket cell overlies the newly emerging bristle rudiment to provide direction for bristle elongation, a...
Guild, Gregory M., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Tilney, Lewis G.
Actin filament bundles can shape cellular extensions into dramatically different forms. We examined cytoskeleton formation during wing hair morphogenesis using both confocal and electron microscopy....
How to make a curved Drosophila bristle using straight actin bundles
Tilney, Lewis G., DeRosier, David J.
This, our Inaugural Article as Academy Members, is ironically our swan song from the field of the actin cytoskeleton. By reviewing what we have learned and what we think is going on during...
Hegan, Peter S., Mermall, Valerie, Tilney, Lewis G., Mooseker, Mark S.
Drosophila myosin IB (Myo1B) is one of two class I myosins in the Drosophila genome. In the larval and adult midgut enterocyte, Myo1B is present within the microvillus (MV) of the apical brush border...
Tilney, Lewis G., Hiramoto, Yukio, Marsland, Douglas
Electron microscope preparations were made of specimens of Actinosphaerium nucleofilum fixed in glutaraldehyde before, during, and after exposure to high pressures (4,000 to 8,000 psi). A study of...
Tilney, Lewis G., Porter, Keith R.
When specimens of Actinosphaerium nucleofilum are placed at 4°C, the axopodia retract and the birefringent core (axoneme) of each axopodium disappears. In fixed specimens, it has been shown that...
A FINE STRUCTURAL ANALYSIS OF CLEAVAGE INDUCTION AND FURROWING IN THE EGGS OF ARBACIA PUNCTULATA
Tilney, Lewis G., Marsland, Douglas
A fine structural study has been carried out on the various formed elements present before, during, and after the first cleavage division, not only in normally developing Arbacia eggs, but also in...
Gibbins, John R., Tilney, Lewis G., Porter, Keith R.
Prior to gastrulation, the microtubules in the presumptive primary mesenchyme cells appear to diverge from points (satellites) in close association with the basal body of the cilium; from here most...
Tilney, Lewis G., Gibbins, John R.
To experimentally test the suggestion made in the preceding paper that the microtubules are involved in cell shape development during the formation and differentiation of the primary mesenchyme, we...
Tilney, Lewis G., Byers, Breck
On the assumption that the double-coiled pattern of microtubules in the axoneme of Echinosphaerium might be due to links of two sizes between adjacent microtubules, we disassembled microtubules with...
Tilney, Lewis G., Goddard, Janette
In the ectodermal cells of sea urchin blastulae, the microtubules converge and appear to make contact with three distinct cytoplasmic foci or satellites associated with the basal body of the cilium....
The axonemes of Raphidiophrys converge near the center of the cell in an electron-opaque material, the centroplast. In order to establish whether this material acts not only to nucleate the...
Tilney, Lewis G., Cardell, Robert R.
Hydrostatic pressure, when applied to segments of the small intestine of the salamander, causes a tremendous reduction in number of microvilli and a loss of the terminal web. The intestinal...
ISOLATION AND REACTIVATION OF THE AXOSTYLE : Evidence for a Dynein-like ATPase in the Axostyle
Mooseker, Mark S., Tilney, Lewis G.
The contractile axostyle is a ribbon-shaped organelle present in certain species of flagellates found in the hindgut of wood eating insects. This organelle propagates an undulatory wave whose motion,...
MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
Tilney, Lewis G., Bryan, Joseph, Bush, Doris J., Fujiwara, Keigi, Mooseker, Mark S., Murphy, Douglas B., ...
When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image...
THE ROLE OF MICROTUBULES IN THE MOVEMENT OF PIGMENT GRANULES IN TELEOST MELANOPHORES
Murphy, Douglas B., Tilney, Lewis G.
When microtubules in teleost melanophores are disrupted with antimitotic agents, colchicine, high hydrostatic pressure, low temperature, and vinblastine, the alignment and movement of the pigment...
Tilney, Lewis G., Hatano, Sadashi, Ishikawa, Harunori, Mooseker, Mark S.
When Asterias or Thyone sperm come in contact with egg jelly, a long process which in Thyone measures up to 90 µm in length is formed from the acrosomal region. This process can be generated in less...
Tilney, Lewis G., Connelly, Patricia S., Vranich, Kelly A., Shaw, Michael K., Guild, Gregory M.
In developing Drosophila bristles two species of cross-linker, the forked proteins and fascin, connect adjacent actin filaments into bundles. Bundles form in three phases: (a) tiny bundles appear;...
Guild, Gregory M., Connelly, Patricia S., Shaw, Michael K., Tilney, Lewis G.
At a late stage in Drosophila oogenesis, nurse cells rapidly expel their cytoplasm into the oocyte via intracellular bridges by a process called nurse cell dumping. Before dumping, numerous cables...
The Plastid of Toxoplasma gondii Is Divided by Association with the Centrosomes
Striepen, Boris, Crawford, Michael J., Shaw, Michael K., Tilney, Lewis G., Seeber, Frank, Roos, David S.
Apicomplexan parasites harbor a single nonphotosynthetic plastid, the apicoplast, which is essential for parasite survival. Exploiting Toxoplasma gondii as an accessible system for cell biological...
Filamin Is Required for Ring Canal Assembly and Actin Organization during Drosophila Oogenesis
Li, Min-gang, Serr, Madeline, Edwards, Kevin, Ludmann, Susan, Yamamoto, Daisuke, Tilney, Lewis G., ...
The remodeling of the actin cytoskeleton is essential for cell migration, cell division, and cell morphogenesis. Actin-binding proteins play a pivotal role in reorganizing the actin cytoskeleton in...
Long continuous actin bundles in Drosophila bristles are constructed by overlapping short filaments
Guild, Gregory M., Connelly, Patricia S., Ruggiero, Linda, Vranich, Kelly A., Tilney, Lewis G.
The actin bundles essential for Drosophila bristle elongation are hundreds of microns long and composed of cross-linked unipolar filaments. These long bundles are built from much shorter modules that...
The road less traveled: transport of Legionella to the endoplasmic reticulum
Roy, Craig R., Tilney, Lewis G.
Phagosomes containing the bacterial pathogen Legionella pneumophila are transported to the ER after macrophage internalization. To modulate phagosome transport, Legionella use a specialized secretion...
Chapman, George B., Tilney, Lewis G.
Entire hydras or tentacles were fixed in OsO4 or in KMnO4 and thereafter washed, dehydrated, and embedded in a methacrylate mixture. Ultrathin sections were cut on an experimental model, thermal...
Cytological Studies of the Nematocysts of Hydra : II. The Stenoteles
Chapman, George B., Tilney, Lewis G.
Entire hydras or tentacles were prepared for electron microscopy as described in the preceding paper. The stenotele capsule has been observed to be composed of an external membrane, a thick chitinous...