Linda S. Thomashow

Mobile genetic elements in the genome of the beneficial rhizobacterium Pseudomonas fluorescensPf-5 (2009)

Mavrodi, Dmitri V, Loper, Joyce E, Paulsen, Ian T, Thomashow, Linda S

Abstract Background Pseudomonas fluorescens Pf-5 is a plant-associated bacterium that inhabits the rhizosphere of a wide variety of plant species and and produces secondary metabolites suppressive of...

Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol (1999)

M. Gita, Linda S. Thomashow

The polyketide metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) is produced by many strains of fluorescent Pseudomonas spp. with biocontrol activity against soilborne fungal plant pathogens. Genes...

Effect of Genetically Modified Pseudomonas putida WCS358r on the Fungal Rhizosphere Microflora of Field-Grown Wheat

Glandorf, Debora C. M., Verheggen, Patrick, Jansen, Timo, Jorritsma, Jan-Willem, Smit, Eric, Leeflang, Paula, ...

We released genetically modified Pseudomonas putida WCS358r into the rhizospheres of wheat plants. The two genetically modified derivatives, genetically modified microorganism (GMM) 2 and GMM 8,...

Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas fluorescens Q2-87

Bangera, M. Gita, Thomashow, Linda S.

The polyketide metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) is produced by many strains of fluorescent Pseudomonas spp. with biocontrol activity against soilborne fungal plant pathogens. Genes...

phzO, a Gene for Biosynthesis of 2-Hydroxylated Phenazine Compounds in Pseudomonas aureofaciens 30-84

Delaney, Shannon M., Mavrodi, Dmitri V., Bonsall, Robert F., Thomashow, Linda S.

Certain strains of root-colonizing fluorescent Pseudomonas spp. produce phenazines, a class of antifungal metabolites that can provide protection against various soilborne root pathogens. Despite the...

Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO1

Mavrodi, Dmitri V., Bonsall, Robert F., Delaney, Shannon M., Soule, Marilyn J., Phillips, Greg, Thomashow, Linda S.

Two seven-gene phenazine biosynthetic loci were cloned from Pseudomonas aeruginosa PAO1. The operons, designated phzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2, are homologous to previously studied...

A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid by Pseudomonas fluorescens 2-79

Mavrodi, Dmitri V., Ksenzenko, Vladimir N., Bonsall, Robert F., Cook, R. James, Boronin, Alexander M., Thomashow, Linda S.

Pseudomonas fluorescens 2-79 produces the broad-spectrum antibiotic phenazine-1-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study, seven genes...

Differential Ability of Genotypes of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens Strains To Colonize the Roots of Pea Plants

Landa, Blanca B., Mavrodi, Olga V., Raaijmakers, Jos M., McSpadden Gardener, Brian B., Thomashow, Linda S., Weller, David M.

Indigenous populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. that occur naturally in suppressive soils are an enormous resource for improving biological...

Production of the Antibiotic Phenazine-1-Carboxylic Acid by Fluorescent Pseudomonas Species in the Rhizosphere of Wheat

Thomashow, Linda S., Weller, David M., Bonsall, Robert F., Pierson, Leland S.

Pseudomonas fluorescens 2-79 and P. aureofaciens 30-84 produce the antibiotic phenazine-1-carboxylic acid and suppress take-all, an important root disease of wheat caused by Gaeumannomyces graminis...

Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens

Blankenfeldt, Wulf, Kuzin, Alexandre P., Skarina, Tatiana, Korniyenko, Yuriy, Tong, Liang, Bayer, Peter, ...

Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic nitrogen-containing metabolites with antibiotic, antitumor, and antiparasitic activity. The antibiotic properties of...

Activation of the phz Operon of Pseudomonas fluorescens 2-79 Requires the LuxR Homolog PhzR, N-(3-OH-Hexanoyl)-l-Homoserine Lactone Produced by the LuxI Homolog PhzI, and a cis-Acting phz Box

Khan, Sharik R., Mavrodi, Dmitri V., Jog, Geetanjali J., Suga, Hiroaki, Thomashow, Linda S., Farrand, Stephen K.

The phz operon of Pseudomonas fluorescens 2-79, which produces phenazine-1-carboxylate, is preceded by two genes, phzR and phzI, that are homologs of quorum-sensing gene pairs of the luxR-luxI...

Effect of Genetically Modified Pseudomonas putida WCS358r on the Fungal Rhizosphere Microflora of Field-Grown Wheat

Glandorf, Debora C. M., Verheggen, Patrick, Jansen, Timo, Jorritsma, Jan-Willem, Smit, Eric, Leeflang, Paula, ...

We released genetically modified Pseudomonas putida WCS358r into the rhizospheres of wheat plants. The two genetically modified derivatives, genetically modified microorganism (GMM) 2 and GMM 8,...

Identification and Characterization of a Gene Cluster for Synthesis of the Polyketide Antibiotic 2,4-Diacetylphloroglucinol from Pseudomonas fluorescens Q2-87

Bangera, M. Gita, Thomashow, Linda S.

The polyketide metabolite 2,4-diacetylphloroglucinol (2,4-DAPG) is produced by many strains of fluorescent Pseudomonas spp. with biocontrol activity against soilborne fungal plant pathogens. Genes...

phzO, a Gene for Biosynthesis of 2-Hydroxylated Phenazine Compounds in Pseudomonas aureofaciens 30-84

Delaney, Shannon M., Mavrodi, Dmitri V., Bonsall, Robert F., Thomashow, Linda S.

Certain strains of root-colonizing fluorescent Pseudomonas spp. produce phenazines, a class of antifungal metabolites that can provide protection against various soilborne root pathogens. Despite the...

Functional Analysis of Genes for Biosynthesis of Pyocyanin and Phenazine-1-Carboxamide from Pseudomonas aeruginosa PAO1

Mavrodi, Dmitri V., Bonsall, Robert F., Delaney, Shannon M., Soule, Marilyn J., Phillips, Greg, Thomashow, Linda S.

Two seven-gene phenazine biosynthetic loci were cloned from Pseudomonas aeruginosa PAO1. The operons, designated phzA1B1C1D1E1F1G1 and phzA2B2C2D2E2F2G2, are homologous to previously studied...

A Seven-Gene Locus for Synthesis of Phenazine-1-Carboxylic Acid by Pseudomonas fluorescens 2-79

Mavrodi, Dmitri V., Ksenzenko, Vladimir N., Bonsall, Robert F., Cook, R. James, Boronin, Alexander M., Thomashow, Linda S.

Pseudomonas fluorescens 2-79 produces the broad-spectrum antibiotic phenazine-1-carboxylic acid (PCA), which is active against a variety of fungal root pathogens. In this study, seven genes...

Differential Ability of Genotypes of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens Strains To Colonize the Roots of Pea Plants

Landa, Blanca B., Mavrodi, Olga V., Raaijmakers, Jos M., McSpadden Gardener, Brian B., Thomashow, Linda S., Weller, David M.

Indigenous populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing fluorescent Pseudomonas spp. that occur naturally in suppressive soils are an enormous resource for improving biological...

Production of the Antibiotic Phenazine-1-Carboxylic Acid by Fluorescent Pseudomonas Species in the Rhizosphere of Wheat

Thomashow, Linda S., Weller, David M., Bonsall, Robert F., Pierson, Leland S.

Pseudomonas fluorescens 2-79 and P. aureofaciens 30-84 produce the antibiotic phenazine-1-carboxylic acid and suppress take-all, an important root disease of wheat caused by Gaeumannomyces graminis...

Structure and function of the phenazine biosynthetic protein PhzF from Pseudomonas fluorescens

Blankenfeldt, Wulf, Kuzin, Alexandre P., Skarina, Tatiana, Korniyenko, Yuriy, Tong, Liang, Bayer, Peter, ...

Phenazines produced by Pseudomonas and Streptomyces spp. are heterocyclic nitrogen-containing metabolites with antibiotic, antitumor, and antiparasitic activity. The antibiotic properties of...

Activation of the phz Operon of Pseudomonas fluorescens 2-79 Requires the LuxR Homolog PhzR, N-(3-OH-Hexanoyl)-l-Homoserine Lactone Produced by the LuxI Homolog PhzI, and a cis-Acting phz Box

Khan, Sharik R., Mavrodi, Dmitri V., Jog, Geetanjali J., Suga, Hiroaki, Thomashow, Linda S., Farrand, Stephen K.

The phz operon of Pseudomonas fluorescens 2-79, which produces phenazine-1-carboxylate, is preceded by two genes, phzR and phzI, that are homologs of quorum-sensing gene pairs of the luxR-luxI...

Role of ptsP, orfT, and sss Recombinase Genes in Root Colonization by Pseudomonas fluorescens Q8r1-96▿

Mavrodi, Olga V., Mavrodi, Dmitri V., Weller, David M., Thomashow, Linda S.

Pseudomonas fluorescens Q8r1-96 produces 2,4-diacetylphloroglucinol (2,4-DAPG), a polyketide antibiotic that suppresses a wide variety of soilborne fungal pathogens, including Gaeumannomyces graminis...

Quantification of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens Strains in the Plant Rhizosphere by Real-Time PCR▿

Mavrodi, Olga V., Mavrodi, Dmitri V., Thomashow, Linda S., Weller, David M.

A real-time PCR SYBR green assay was developed to quantify populations of 2,4-diacetylphloroglucinol (2,4-DAPG)-producing (phlD+) strains of Pseudomonas fluorescens in soil and the rhizosphere....

The purification, crystallization and preliminary structural characterization of PhzM, a phenazine-modifying methyltransferase from Pseudomonas aeruginosa

Gohain, Neelakshi, Thomashow, Linda S., Mavrodi, Dmitri V., Blankenfeldt, Wulf

PhzM, an S-adenosylmethionine-dependent methyltransferase enzyme that catalyzes a reaction involved in the biosynthesis of pyocyanin in P. aeruginosa, was cloned, overexpressed and crystallized. Data...

The purification, crystallization and preliminary structural characterization of FAD-dependent monooxygenase PhzS, a phenazine-modifying enzyme from Pseudomonas aeruginosa

Gohain, Neelakshi, Thomashow, Linda S., Mavrodi, Dmitri V., Blankenfeldt, Wulf

PhzS, an FAD-dependent monooxygenase that catalyzes a reaction involved in the biosynthesis of the virulence factor pyocyanin in P. aeruginosa, was cloned, overexpressed and crystallized. Data...