Wortmannin induces homotypic fusion of plant prevacuolar compartments (2009)
Wang, Junqi, Cai, Yi, Miao, Yansong, Lam, Sheung Kwan, Jiang, Liwen
Wortmannin, a specific inhibitor of phosphatidyl-inositol 3-kinase, is a useful tool for studying protein trafficking and identifying organelles in the plant secretory and endocytic pathways. It has...
Wang, Junqi, Suen, Pui Kit, Xu, Zeng-Fu, Jiang, Liwen
Sucrose binding proteins (SBPs) were predicted to be membrane-associated, but have been shown to localize in the lumen of protein storage vacuoles of various seeds. In this study, a new 64 kDa SBP...
Lai, Kwok Wai, Yau, Chi Ping, Tse, Yu Chung, Jiang, Liwen, Yip, Wing Kin
While most dicot plants produce little ethylene in their vegetative stage, many monocots such as rice liberate a relatively large amount of ethylene with cyanide as a co-product in their seedling...
Wang, Min-Long, Hsu, Chia-Mei, Chang, Liang-Chi, Wang, Co-Shine, Su, Ting-Ho, Huang, Yih-Jong John, ...
In lily (Lilium longiflorum cv. Avita) pollen cold-stored (–20°C) for 2 months, typical in vitro germination/growth was delayed by about 1 h compared with fresh pollen. We hypothesized that some...
BP-80 and Homologs are Concentrated on Post-Golgi, Probable Lytic Prevacuolar Compartments (2002)
Li, Yu-Bing, Rogers, Sally W., Tse, Yu Chung, Lo, Sze Wan, Sun, Samuel S. M., Jauh, Guang-Yuh, ...
Prevacuolar compartments (PVCs) are membrane-bound organelles that mediate protein traffic between Golgi and vacuoles in the plant secretory pathway. Here we identify and define organelles as the...
Jiang, Liwen, Kermode, Allison R., Jones, Russell L.
The effect of premature drying on the sensitivity of aleurone layer cells of developing barley (Hordeum vulgare L.) grain to gibberellic acid (GA3) was investigated. The capacity of barley aleurone...
Sohn, Eun Ju, Kim, Eol Sun, Zhao, Min, Kim, Soo Jin, Kim, Hyeran, Kim, Yong-Woo, ...
Rab proteins are members of the Ras superfamily of small GTP binding proteins and play important roles in various intracellular trafficking steps. We investigated the role of Rha1, an Arabidopsis...
Jin, Jing Bo, Bae, Hyeunjong, Kim, Soo Jin, Jin, Yin Hua, Goh, Chang-Hyo, Kim, Dae Heon, ...
Dynamin-related proteins are high molecular weight GTP binding proteins and have been implicated in various biological processes. Here, we report the functional characterization of two dynamin...
Tse, Yu Chung, Mo, Beixin, Hillmer, Stefan, Zhao, Min, Lo, Sze Wan, Robinson, David G., ...
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs). We have demonstrated recently that vacuolar sorting receptor (VSR) proteins are concentrated on...
Oufattole, Mohammed, Park, Joon Ho, Poxleitner, Marianne, Jiang, Liwen, Rogers, John C.
In plant cells, certain membrane proteins move by unknown mechanisms directly from the endoplasmic reticulum (ER) to prevacuolar or vacuole-like organelles where membrane is internalized to form a...
Sohn, Eun Ju, Kim, Eol Sun, Zhao, Min, Kim, Soo Jin, Kim, Hyeran, Kim, Yong-Woo, ...
Rab proteins are members of the Ras superfamily of small GTP binding proteins and play important roles in various intracellular trafficking steps. We investigated the role of Rha1, an Arabidopsis...
Jin, Jing Bo, Bae, Hyeunjong, Kim, Soo Jin, Jin, Yin Hua, Goh, Chang-Hyo, Kim, Dae Heon, ...
Dynamin-related proteins are high molecular weight GTP binding proteins and have been implicated in various biological processes. Here, we report the functional characterization of two dynamin...
Tse, Yu Chung, Mo, Beixin, Hillmer, Stefan, Zhao, Min, Lo, Sze Wan, Robinson, David G., ...
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs). We have demonstrated recently that vacuolar sorting receptor (VSR) proteins are concentrated on...
Oufattole, Mohammed, Park, Joon Ho, Poxleitner, Marianne, Jiang, Liwen, Rogers, John C.
In plant cells, certain membrane proteins move by unknown mechanisms directly from the endoplasmic reticulum (ER) to prevacuolar or vacuole-like organelles where membrane is internalized to form a...
Oliviusson, Peter, Heinzerling, Oliver, Hillmer, Stefan, Hinz, Giselbert, Tse, Yu Chung, Jiang, Liwen, ...
Receptors for acid hydrolases destined for the lytic compartment in yeast and mammalian cells are retrieved from intermediate, endosomal organelles with the help of a pentameric protein complex...
Miao, Yansong, Yan, Pak Kan, Kim, Hyeran, Hwang, Inhwan, Jiang, Liwen
We have previously demonstrated that vacuolar sorting receptor (VSR) proteins are concentrated on prevacuolar compartments (PVCs) in plant cells. PVCs in tobacco (Nicotiana tabacum) BY-2 cells are...
Dynamic Response of Prevacuolar Compartments to Brefeldin A in Plant Cells1[W][OA]
Tse, Yu Chung, Lo, Sze Wan, Hillmer, Stefan, Dupree, Paul, Jiang, Liwen
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs) in the secretory pathway. Using transgenic tobacco (Nicotiana tabacum) Bright-Yellow-2 (BY-2)...
Lam, Sheung Kwan, Siu, Ching Lung, Hillmer, Stefan, Jang, Seonghoe, An, Gynheung, Robinson, David G., ...
We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to the lytic vacuole in tobacco (Nicotiana tabacum) BY-2 cells....
Wang, Junqi, Li, Yubing, Lo, Sze Wan, Hillmer, Stefan, Sun, Samuel S.M., Robinson, David G., ...
Plants accumulate and store proteins in protein storage vacuoles (PSVs) during seed development and maturation. Upon seed germination, these storage proteins are mobilized to provide nutrients for...
Min, Myung Ki, Kim, Soo Jin, Miao, Yansong, Shin, Juyoun, Jiang, Liwen, Hwang, Inhwan
ADP ribosylation factor (Arf) GTPase-activating proteins (GAPs) promote the hydrolysis of GTP bound to Arfs to GDP, which plays a pivotal role in regulating Arfs by converting the active GTP-bound...
Integral Membrane Protein Sorting to Vacuoles in Plant Cells: Evidence for Two Pathways
Plant cells may contain two functionally distinct vacuolar compartments. Membranes of protein storage vacuoles (PSV) are marked by the presence of α-tonoplast intrinsic protein (TIP), whereas lytic...
The protein storage vacuole: a unique compound organelle
Jiang, Liwen, Phillips, Thomas E., Hamm, Christopher A., Drozdowicz, Yolanda M., Rea, Philip A., Maeshima, Masayoshi, ...
Storage proteins are deposited into protein storage vacuoles (PSVs) during plant seed development and maturation and stably accumulate to high levels; subsequently, during germination the storage...
Biogenesis of the Protein Storage Vacuole Crystalloid
Jiang, Liwen, Phillips, Thomas E., Rogers, Sally W., Rogers, John C.
We identify new organelles associated with the vacuolar system in plant cells. These organelles are defined biochemically by their internal content of three integral membrane proteins: a chimeric...
PICK1 deficiency causes male infertility in mice by disrupting acrosome formation
Xiao, Nan, Kam, Chuen, Shen, Chong, Jin, Wenying, Wang, Junqi, Lee, Kwong Man, ...
Protein interacting with C kinase 1 (PICK1) is a peripheral membrane protein involved in protein trafficking, a function that has been well characterized in neurons. Here, we report that male mice...
SCAMPs Highlight the Developing Cell Plate during Cytokinesis in Tobacco BY-2 Cells1[C][W][OA]
Lam, Sheung Kwan, Cai, Yi, Hillmer, Stefan, Robinson, David G., Jiang, Liwen
We previously demonstrated that rice (Oryza sativa) SECRETORY CARRIER MEMBRANE PROTEIN1 (OsSCAMP1)-yellow fluorescent protein in transgenic tobacco (Nicotiana tabacum) Bright Yellow-2 cells locates...
Tse, Yu Chung, Lam, Sheung Kwan, Jiang, Liwen
The fungal macrocyclic lactone brefeldin A (BFA) has been a useful tool in studying protein trafficking in the secretory and endocytic pathways in plant cells. The development of various GFP-tagged...
Wang, Junqi, Suen, Pui Kit, Xu, Zeng-Fu, Jiang, Liwen
Sucrose binding proteins (SBPs) were predicted to be membrane-associated, but have been shown to localize in the lumen of protein storage vacuoles of various seeds. In this study, a new 64 kDa SBP...
Lai, Kwok Wai, Yau, Chi Ping, Tse, Yu Chung, Jiang, Liwen, Yip, Wing Kin
While most dicot plants produce little ethylene in their vegetative stage, many monocots such as rice liberate a relatively large amount of ethylene with cyanide as a co-product in their seedling...
Wortmannin induces homotypic fusion of plant prevacuolar compartments*
Wang, Junqi, Cai, Yi, Miao, Yansong, Lam, Sheung Kwan, Jiang, Liwen
Wortmannin, a specific inhibitor of phosphatidyl-inositol 3-kinase, is a useful tool for studying protein trafficking and identifying organelles in the plant secretory and endocytic pathways. It has...