A comparative study of recombinant and native frutalin binding to human prostate tissues (2009)
Oliveira, Carla, Teixeira, José A, Schmitt, Fernando, Domingues, Lucília
Abstract Background Numerous studies indicate that cancer cells present an aberrant glycosylation pattern that can be detected by lectin histochemistry. Lectins have shown the ability to recognise...
Guimarães, Pedro M. R., Le Berre, V., Sokol, Serguei, François, J., Teixeira, J. A., Domingues, Lucília
The engineering of Saccharomyces cerevisiae strains for lactose utilization has been attempted with the intent of developing high productivity processes for alcoholic fermentation of cheese whey. A...
Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília
The development of microorganims that efficiently ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous bio-ethanol production....
Moreira, Susana M, Andrade, Fábia K., Domingues, Lucília, Gama, Miguel
Several approaches can be used to functionalize biomaterials, such as hydrogels, for biomedical applications. One of the molecules often used to improve cells adhesion is the peptide Arg-Gly-Asp...
Identification of coalho cheese microbiot of districts of Pernambuco, Brazil (2008)
Neto, Torquato M. S., Teixeira, J. A., Domingues, Lucília, Lima-Filho, José. L., Porto, Ana L.. F.
Lactose fermentation by recombinant Saccharomyces cerevisiae strains (2008)
Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília
The development of Saccharomyces cerevisiae strains with the ability to ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous...
Virtual laboratories in (bio)chemical engineering education (2008)
Domingues, Lucília, Rocha, I., Dourado, Fernando, Alves, M. M., Ferreira, E. C.
"Qualidade” of the Universidade do Minho.
Felix, W., Moreira, R. A., Teixeira, J. A., Domingues, Lucília
Frutalin is an α-d-galactose-binding lectin expressed in breadfruit seeds. Its isolation from plant is time-consuming and results in a heterogeneous mixture of different lectin isoforms. In order to...
Differentiation of human pre-adipocytes by recombinant adiponectin (2008)
Avides, Maria Do Carmo, Domingues, Lucília, Vicente, A. A., Teixeira, J. A.
Obesity has become a global health problem and it is linked to a higher risk of diseases and metabolic disorders such as diabetes, cardiovascular disease, and cancer. The adipose tissue plays an...
Gama, F. M., Domingues, Lucília
Antimicrobial peptides (AMPs) are molecules that act in a wide range of physiological defensive mechanisms developed to counteract bacteria, fungi, parasites and viruses. Several hundreds of AMPs...
Guimarães, Pedro M. R., Multanen, Jyri-Pekka, Domingues, Lucília, Teixeira, J. A., Londesborough, John
Initial rates of sugar uptake (zero-trans rates) are often measured by incubating yeast cells with radiolabeled sugars for 5 to 30 s and determining the radioactivity entering the cells. The yeast...
Cunha, M. R. M., Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília
Ethanol is well known as a toxic metabolite for yeast cells. Thus, strains that can grow well under high ethanol stress condition are highly desirable. This work aims to select and characterize...
Adaptive evolution of a lactose-consuming saccharomyces cerevisiae recombinant (2008)
Guimarães, P., François, J., Parrou, J. L., Teixeira, J. A., Domingues, Lucília
The construction of Saccharomyces cerevisiae strains that ferment lactose has biotechnological interest, particularly for cheese whey fermentation. A flocculent lactose-consuming S. cerevisiae...
Alcoholic fermentation of lactose by engineered flocculent Saccharomyces cerevisiae (2008)
Guimarães, Pedro M. R., Teixeira, J. A., Domingues, Lucília
The construction of Saccharomyces cerevisiae strains with the ability to ferment lactose has biotechnological interest, particularly for cheese whey fermentation to ethanol. Direct fermentation of...
Expression of frutalin in Pichia pastoris and evaluation of its potential as a cancer marker (2007)
Duarte, M. L., Felix, W., Moreira, R. A., Teixeira, J. A., Schmitt, Fernando C., ...
Ribeiro, Orquídea, Gombert, Andreas Karoly, Teixeira, J. A., Domingues, Lucília
The yeast Kluyveromyces marxianus presents several interesting features that make this species a promising industrial yeast for the production of several compounds. In order to take full advantage of...
Moreira, Susana, Domingues, Lucília, Gama, F. M., Casal, Margarida
Fundação para a Ciência e a Tecnologia (FCT) - POCTI/BIO/45356/2002 ; (SFRH/BD/18418/2004).
Adaptive evolution of a lactose-consuming saccharomyces cerevisiae recombinant (2007)
Guimarães, P., François, J., Parrou, J. L., Teixeira, J. A., Domingues, Lucília
The construction of Saccharomyces cerevisiae strains with the ability to efficiently ferment lactose has biotechnological interest, particularly for the alcoholic fermentation of cheese whey (a high...
Multiple gene disruption and marker rescue in the yeast Kluyveromyces marxianus (2007)
Ribeiro, Orquídea, Gombert, Andreas Karoly, Teixeira, J. A., Domingues, Lucília
Teixeira, J. A., Lima, Nelson, Silva, Nancy A. Da, Domingues, Lucília
A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast...
Kinetics of lactose fermentation using a recombinant saccharomyces cerevisiae strain (2006)
Juraščík, Martin, Guimarães, P., Klein, Jaroslav, Domingues, Lucília, Teixeira, J. A., Markoš, J.
This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the...
Adaptive evolution of a recombinant lactose-consuming Saccharomyces cerevisiae strain (2006)
Guimarães, P., Parrou, J. L., Le Berre, V., François, J., Teixeira, J. A., Domingues, Lucília
In previous work, a recombinant S. cerevisiae flocculent strain (NCYC869-A3/T1, or simply T1) with the ability to express both the LAC4 (coding for beta-galactosidase) and LAC12 (lactose permease)...
Generation of gene disruption cassettes with long flanking regions via fusion PCR (2005)
Gombert, Andreas Karoly, Johansson, Björn, Lima, Nelson, Teixeira, J. A., Domingues, Lucília
Casulari,Luiz Augusto, Celotti,Fábio, Naves,Luciana A., Domingues,Lucília, Papadia,Carla
Long term use of high doses of estrogen and the presence of chronic hyperprolactinemia may, at least in the rat, provoke lesions in the tuberoinfundibular dopaminergic (TIDA) neurons responsible for...
Molecular yeast solutions for whey bioremediation (2005)
Domingues, Lucília, Guimarães, P., Klein, Jaroslav, Teixeira, J. A., Lima, Nelson
Fundação para a Ciência e a Tecnologia (FCT)
Kinetics of lactose fermentation using recombinant saccharomyces cerevisiae (2005)
Juraščík, Martin, Guimarães, P., Klein, Jaroslav, Domingues, Lucília, Teixeira, J. A., Markoš, J.
Slovak Scientific Grand Agency; FCT.
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.
The continuous production of extracellular heterologous beta-galactosidase by a recombinant flocculating Saccharomyces cerevisiae, expressing the lacA gene (coding for beta-galactosidase) of...
Guimarães, P., Klein, Jaroslav, Domingues, Lucília, Teixeira, J. A.
In recent years, there has been a growing interest in bioreactors utilizing immobilized or flocculating cells in continuous process in order to improve the bioprocess productivity. One of possible...
Klein, Jaroslav, Maia, J. M., Vicente, A. A., Domingues, Lucília, Teixeira, J. A., Juraščík, Martin
In this article a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high-celldensity system is presented. A highly flocculating recombinant strain of Sacharomyces...
Cloning and expression of carbohydrate binding module in Pichia pastoris (2005)
Moreira, Susana, Domingues, Lucília, Gama, F. M., Casal, Margarida
The main goal of this work is the production of recombinant biologically active peptides fused with a Carbohydrate Binding Module (CBM). Aiming at the optimization of large scale expression, CBM...
Klein, Jaroslav, Maia, J. M., Juraščík, Martin, Vicente, A. A., Domingues, Lucília, Teixeira, J. A.
European Community programme Improving Human Research Potential.
Klein, Jaroslav, Vicente, A. A., Maia, J. M., Domingues, Lucília, Teixeira, J. A.
In this paper a hydrodynamic and rheological analysis of a continuous airlift bioreactor with high cell density system is presented. A highly flocculating recombinant strain of Sacharomyces cerevisae...
Production of β-galactosidase from recombinant Saccharomyces cerevisiae grown on lactose (2004)
Domingues, Lucília, Castro, I., Lima, Nelson, Teixeira, J. A.
Improved productivity and costs reduction in fermentation processes may be attained by using flocculating cell cultures. The production of extracellular heterologous β-galactosidase by recombinant...
Klein, Jaroslav, Vicente, A. A., Domingues, Lucília, Juraščík, Martin, Teixeira, J. A.
One of the most common ways to improve the productivity of a fermentation process is the use of high cell density systems. In practice, such a system usually represents a threephase...
Mangieri,Paola, Suzuki,Kunio, Ferreira,Moema, Domingues,Lucília, Casulari,Luiz Augusto
It is well known that the central nervous system (CNS) influences the pituitary hormone secretions and that diseases of CNS are frequently associated with an altered endocrine function. The aim of...
An integrated approach for cheese whey lactose valorisation (2003)
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.
The development of any fermentation process requires the integration of life and engineering sciences with the aim of obtaining a high productivity system. The use of immobilized cells is, without...
Domingues, Lucília, Teixeira, J. A., Penttilä, M., Lima, Nelson
A flocculent Saccharomyces cerevisiae strain secreting Aspergillus niger β-galactosidase activity was constructed by transforming S. cerevisiae NCYC869-A3 strain with plasmid pVK1.1 harboring the A....
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.
Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for β-galactosidase) and LAC12 (coding for...
Tese de doutoramento em Engenharia Química e Biológica
Contamination of a high-cell-density continuous bioreactor (2000)
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.
Continuous fermentations were carried out with a recombinant flocculent Saccharomyces cerevisiae strain in an airlift bioreactor. Once operating under steady state at a dilution rate of 0.45 h−1,...
Domingues, Lucília, Teixeira, J. A., Lima, Nelson, Penttilä, M.
One way of improving heterologous protein production is to use high cell density systems, one of the most attractive being the flocculating yeast production system. Also, lactose is available in...
Applications of yeast flocculation in biotechnological processes (2000)
Domingues, Lucília, Vicente, A. A., Lima, Nelson, Teixeira, J. A.
A review on the main aspects associated with yeast flocculation and its application in biotechnological processes is presented. This subject is addressed following three main aspects – the basics...
Domingues, Lucília, Dantas, Maria M., Lima, Nelson, Teixeira, J. A.
Alcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease)...
Continuous ethanol production from cheese whey permeate (1999)
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.
Praxis XXI - BD/11306/97.
Construction of a flocculent saccharomyces cerevisiae fermenting lactose (1999)
Domingues, Lucília, Teixeira, J. A., Lima, Nelson
A flocculent Saccharomyces cerevisiae strain with the ability to express both the LAC4 (coding for b-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus was...
Transformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4 (1999)
Venâncio, Armando, Domingues, Lucília, Lima, Nelson
A flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single-stranded carrier DNA using the shuttle vector...
Novas metodologias para a fermentação alcoólica do soro de queijo (1999)
Domingues, Lucília, Lima, Nelson, Teixeira, J. A.
Em Portugal, são diariamente lançados no meio ambiente cerca de 1 milhão de litros de soro de queijo – fracção líquida resultante do fabrico de queijo. O impacte ambiental ocasionado por tal...
Domingues, Lucília, Dantas, Maria M., Lima, Nelson, Teixeira, J. A.
Praxis XXI.
Domingues, Lucília, Teixeira, J. A., Lima, Nelson, Penttilä, M.
Praxis XXI (BD/11306/97).
Domingues, Lucília, Teixeira, J. A., Lima, Nelson
Yeast β-galactosidase activity was detected by a microtiter plate assay using pNPG or Xgal as substrate in 30 minutes. The detection gave a clear result which is well correlated with the specific...
Protein partition on a derivative guar gum based aqueous two-phase system (1995)
Venâncio, Armando, Almeida, Catarina, Domingues, Lucília, Teixeira, J. A.
A new aqueous two-phase system based on a guar gum derivative, commercialized under the trade name of Solvitose Gum Ofa, and poly(ethylene glycol) is described. Its main difference from other...
Adaptive Evolution of a Lactose-Consuming Saccharomyces cerevisiae Recombinant▿
Guimarães, Pedro M. R., François, Jean, Parrou, Jean Luc, Teixeira, José A., Domingues, Lucília
The construction of Saccharomyces cerevisiae strains that ferment lactose has biotechnological interest, particularly for cheese whey fermentation. A flocculent lactose-consuming S. cerevisiae...
Guimarães, Pedro M. R., Multanen, Jyri-Pekka, Domingues, Lucília, Teixeira, José A., Londesborough, John
Initial rates of sugar uptake (zero-trans rates) are often measured by incubating yeast cells with radiolabeled sugars for 5 to 30 s and determining the radioactivity entering the cells. The yeast...