M. A. Gonda

Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon.

Banai, M, Gonda, M A, Ranhand, J M, LeBlanc, D J

Streptococcus faecalis R plasmid pJH1 did not transform competent strains of Streptococcus sanguis. A hybrid plasmid, pDL310, consisting of virtually all of the S. faecalis hemolysin-bacteriocin...

Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene.

Battles, J K, Williamson, J C, Pike, K M, Gorelick, P L, Ward, J M, Gonda, M A

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to...

Amino acid sequence analysis of the proteolytic cleavage products of the bovine immunodeficiency virus Gag precursor polypeptide.

Tobin, G J, Sowder, R C, Fabris, D, Hu, M Y, Battles, J K, Fenselau, C, ...

Bovine immunodeficiency virus Gag proteins were purified from virions, and their amino acid sequences and molecular masses were determined. The matrix, capsid, and nucleocapsid (MA, CA, and NC,...

Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein.

Oberste, M S, Williamson, J C, Greenwood, J D, Nagashima, K, Copeland, T D, Gonda, M A

One of the six putative accessory genes of bovine immunodeficiency virus (BIV) is similar to those identified as rev in the human immunodeficiency virus and visna virus genomes. To further analyze...

Pathogenicity of molecularly cloned bovine leukemia virus.

Rovnak, J, Boyd, A L, Casey, J W, Gonda, M A, Jensen, W A, Cockerell, G L

To delineate the mechanisms of bovine leukemia virus (BLV) pathogenesis, four full-length BLV clones, 1, 8, 9, and 13, derived from the transformed cell line FLK-BLV and a clone construct, pBLV913,...

Immunological characterization of the gag gene products of bovine immunodeficiency virus.

Battles, J K, Hu, M Y, Rasmussen, L, Tobin, G J, Gonda, M A

The bovine immunodeficiency virus (BIV) gag gene encodes a 53-kDa precursor (Pr53gag) that is involved in virus particle assembly and is further processed into the putative matrix (MA), capsid (CA),...

Bovine immunodeficiency-like virus encodes factors which trans activate the long terminal repeat.

Pallansch, L A, Lackman-Smith, C S, Gonda, M A

Lentiviruses are known to encode factors which trans activate expression from the viral long terminal repeat (LTR); the primary trans activator is the tat gene product. One of the putative accessory...

Persistent infection of rabbits with bovine immunodeficiency-like virus.

Pifat, D Y, Ennis, W H, Ward, J M, Oberste, M S, Gonda, M A

Chronic infection of rabbits was induced by a single intraperitoneal injection of bovine immunodeficiency-like virus (BIV)-infected cells. Ten BIV-infected animals were monitored serologically for up...

Analysis of the transcription pattern and mapping of the putative rev and env splice junctions of bovine immunodeficiency-like virus.

Oberste, M S, Greenwood, J D, Gonda, M A

The bovine immunodeficiency-like virus (BIV) genome contains the obligatory structural genes of all retroviruses and, in addition, the complex central region of lentiviruses; this novel region may...

Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses.

Pyper, J M, Clements, J E, Gonda, M A, Narayan, O

Caprine arthritis encephalitis virus (CAEV) is an exogenous, nononcogenic retrovirus which causes neurological disease and crippling arthritis in goats. A complete CAEV genome was cloned from...

Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.

Baker, C C, Phelps, W C, Lindgren, V, Braun, M J, Gonda, M A, Howley, P M

We cloned and analyzed the integrated human papillomavirus type 16 (HPV-16) genomes that are present in the human cervical carcinoma cell lines SiHa and CaSki. The single HPV-16 genome in the SiHa...

Heteroduplex analysis of molecular clones of the pathogenic Friend virus complex: Friend murine leukemia virus, Friend mink cell focus-forming virus, and the polycythemia- and anemia-inducing strains of Friend spleen focus-forming virus.

Gonda, M A, Kaminchick, J, Oliff, A, Menke, J, Nagashima, K, Scolnick, E M

The pathogenic Friend virus complex is of considerable interest in that, although members of this group are genetically related, they differ markedly in biochemical and biological properties....

Subcellular localization of glycoproteins encoded by the viral oncogene v-fms.

Anderson, S J, Gonda, M A, Rettenmier, C W, Sherr, C J

The McDonough strain of feline sarcoma virus encodes a polyprotein that is cotranslationally glycosylated and proteolytically cleaved to yield transforming glycoproteins specified by the viral...

The visna virus genome: evidence for a hypervariable site in the env gene and sequence homology among lentivirus envelope proteins.

Braun, M J, Clements, J E, Gonda, M A

The complete nucleotide sequence of the visna virus 1514 genome was determined. Our sequence confirms the relationship of visna virus and other lentiviruses to human immunodeficiency virus (HIV) both...

Molecular cloning of a family of retroviral sequences found in chimpanzee but not human DNA.

Bonner, T I, Birkenmeier, E H, Gonda, M A, Mark, G E, Searfoss, G H, Todaro, G J

A number of retrovirus-like sequences have been cloned from chimpanzee DNA which constitute the chimpanzee homologs of the endogenous colobus type C virus CPC-1. One of the clones contains a nearly...

Molecular cloning, genomic analysis, and biological properties of rat leukemia virus and the onc sequences of Rasheed rat sarcoma virus.

Gonda, M A, Young, H A, Elser, J E, Rasheed, S, Talmadge, C B, Nagashima, K, ...

Rasheed rat sarcoma virus (RaSV) has been shown to code for a protein of 29,000 Mr not present in replication-competent rat type C helper virus (RaLV)-infected cells. This protein is a fused gene...

Genome structure of mink cell focus-forming murine leukemia virus in epithelial mink lung cells transformed vitro by iododeoxyuridine-induced C3H/MuLV cells.

Rapp, U R, Birkenmeier, E, Bonner, T I, Gonda, M A, Gunnell, M

We characterized mink cell focus-forming murine leukemia viruses that were isolated from C3H/MCA-5 cells after induction with 5-iododeoxyuridine in culture. Mink lung epithelial cells malignantly...

Avian reticuloendotheliosis virus: characterization of the high-molecular-weight viral RNA in transforming and helper virus populations.

Gonda, M A, Rice, N R, Gilden, R V

Reticuloendotheliosis virus strain T (REV-T) is a type C retrovirus known to transform avian fibroblasts, spleen cells, and bone marrow cells and to produce virulent reticuloendotheliosis in young...

Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector.

Karacostas, V, Nagashima, K, Gonda, M A, Moss, B

Infectious retrovirus particles consist of a core structure containing RNA and gag-pol polypeptides surrounded by a lipid membrane studded with env proteins. A recombinant vaccinia virus was designed...

Sequence of MET protooncogene cDNA has features characteristic of the tyrosine kinase family of growth-factor receptors.

Park, M, Dean, M, Kaul, K, Braun, M J, Gonda, M A, Vande Woude, G

We isolated overlapping cDNA clones corresponding to the major MET protooncogene transcript. The cDNA nucleotide sequence contained an open reading frame of 1408 amino acids with features...

Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine.

Arthur, L O, Pyle, S W, Nara, P L, Bess, J W, Gonda, M A, Kelliher, J C, ...

The major envelope glycoprotein of a human immunodeficiency virus (HIV) has been purified and was utilized as a prototype vaccine in chimpanzees. The 120,000-dalton glycoprotein (gp120) was purified...

Absence of cytotoxic antibody to human immunodeficiency virus-infected cells in humans and its induction in animals after infection or immunization with purified envelope glycoprotein gp120.

Nara, P L, Robey, W G, Gonda, M A, Carter, S G, Fischinger, P J

The presence of antibody-dependent complement-mediated cytotoxicity (ACC) was assessed in humans and chimpanzees, which are capable of infection with human immunodeficiency virus isolate HTLV-IIIb,...

Analysis of two divergent rat genomic clones homologous to the transforming gene of Harvey murine sarcoma virus.

DeFeo, D, Gonda, M A, Young, H A, Chang, E H, Lowy, D R, Scolnick, E M, ...

Harvey murine sarcoma virus (Ha-MuSV) is a mouse-rat recombinant retrovirus that encodes a protein designated p21, required for virally induced transformation. Using a radiolabeled DNA fragment from...

Human T-cell lymphotropic virus type III shares sequence homology with a family of pathogenic lentiviruses.

Gonda, M A, Braun, M J, Clements, J E, Pyper, J M, Wong-Staal, F, Gallo, R C, ...

The etiologic agent of the acquired immune deficiency syndrome, human T-cell lymphotropic virus type III (HTLV-III), has recently been shown to morphologically resemble and share sequence homology...

Stability of RNA stem-loop structure and distribution of non-random structure in the human immunodeficiency virus (HIV-I).

Le, S Y, Chen, J H, Braun, M J, Gonda, M A, Maizel, J V

The stability of potential RNA stem-loop structures in human immunodeficiency virus isolates, HTLV-III and ARV, has been calculated, and the relevance to the local significant secondary structures in...

Genomes of evolutionarily divergent members of the human T-cell leukemia virus family (HTLV-I and HTLV-II) are highly conserved, especially in pX.

Shaw, G M, Gonda, M A, Flickinger, G H, Hahn, B H, Gallo, R C, Wong-Staal, F

Human T-cell leukemia virus (HTLV) is a family of related human T-lymphotropic retroviruses closely linked with certain human T-cell malignancies and associated with many cases of acquired...

Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses.

Chang, E H, Gonda, M A, Ellis, R W, Scolnick, E M, Lowy, D R

Harvey and Kirsten murine sarcoma viruses each encode a structurally and functionally related 21-kilodalton protein (p21), which is the transforming protein of each virus. Using probes from the...

Genomic diversity of the acquired immune deficiency syndrome virus HTLV-III: different viruses exhibit greatest divergence in their envelope genes.

Hahn, B H, Gonda, M A, Shaw, G M, Popovic, M, Hoxie, J A, Gallo, R C, ...

Converging lines of research have linked human T-cell lymphotropic virus type III (HTLV-III) to the pathogenesis of the acquired immune deficiency syndrome. A characteristic feature of this virus is...

Streptococcus faecalis R plasmid pJH1 contains a pAM alpha 1 delta 1-like replicon.

Banai, M, Gonda, M A, Ranhand, J M, LeBlanc, D J

Streptococcus faecalis R plasmid pJH1 did not transform competent strains of Streptococcus sanguis. A hybrid plasmid, pDL310, consisting of virtually all of the S. faecalis hemolysin-bacteriocin...

Diagnostic assay for Helicobacter hepaticus based on nucleotide sequence of its 16S rRNA gene.

Battles, J K, Williamson, J C, Pike, K M, Gorelick, P L, Ward, J M, Gonda, M A

Conserved primers were used to PCR amplify 95% of the Helicobacter hepaticus 16S rRNA gene. Its sequence was determined and aligned to those of related bacteria, enabling the selection of primers to...

Amino acid sequence analysis of the proteolytic cleavage products of the bovine immunodeficiency virus Gag precursor polypeptide.

Tobin, G J, Sowder, R C, Fabris, D, Hu, M Y, Battles, J K, Fenselau, C, ...

Bovine immunodeficiency virus Gag proteins were purified from virions, and their amino acid sequences and molecular masses were determined. The matrix, capsid, and nucleocapsid (MA, CA, and NC,...

Characterization of bovine immunodeficiency virus rev cDNAs and identification and subcellular localization of the Rev protein.

Oberste, M S, Williamson, J C, Greenwood, J D, Nagashima, K, Copeland, T D, Gonda, M A

One of the six putative accessory genes of bovine immunodeficiency virus (BIV) is similar to those identified as rev in the human immunodeficiency virus and visna virus genomes. To further analyze...

Pathogenicity of molecularly cloned bovine leukemia virus.

Rovnak, J, Boyd, A L, Casey, J W, Gonda, M A, Jensen, W A, Cockerell, G L

To delineate the mechanisms of bovine leukemia virus (BLV) pathogenesis, four full-length BLV clones, 1, 8, 9, and 13, derived from the transformed cell line FLK-BLV and a clone construct, pBLV913,...

Immunological characterization of the gag gene products of bovine immunodeficiency virus.

Battles, J K, Hu, M Y, Rasmussen, L, Tobin, G J, Gonda, M A

The bovine immunodeficiency virus (BIV) gag gene encodes a 53-kDa precursor (Pr53gag) that is involved in virus particle assembly and is further processed into the putative matrix (MA), capsid (CA),...

Bovine immunodeficiency-like virus encodes factors which trans activate the long terminal repeat.

Pallansch, L A, Lackman-Smith, C S, Gonda, M A

Lentiviruses are known to encode factors which trans activate expression from the viral long terminal repeat (LTR); the primary trans activator is the tat gene product. One of the putative accessory...

Persistent infection of rabbits with bovine immunodeficiency-like virus.

Pifat, D Y, Ennis, W H, Ward, J M, Oberste, M S, Gonda, M A

Chronic infection of rabbits was induced by a single intraperitoneal injection of bovine immunodeficiency-like virus (BIV)-infected cells. Ten BIV-infected animals were monitored serologically for up...

Analysis of the transcription pattern and mapping of the putative rev and env splice junctions of bovine immunodeficiency-like virus.

Oberste, M S, Greenwood, J D, Gonda, M A

The bovine immunodeficiency-like virus (BIV) genome contains the obligatory structural genes of all retroviruses and, in addition, the complex central region of lentiviruses; this novel region may...

Sequence homology between cloned caprine arthritis encephalitis virus and visna virus, two neurotropic lentiviruses.

Pyper, J M, Clements, J E, Gonda, M A, Narayan, O

Caprine arthritis encephalitis virus (CAEV) is an exogenous, nononcogenic retrovirus which causes neurological disease and crippling arthritis in goats. A complete CAEV genome was cloned from...

Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.

Baker, C C, Phelps, W C, Lindgren, V, Braun, M J, Gonda, M A, Howley, P M

We cloned and analyzed the integrated human papillomavirus type 16 (HPV-16) genomes that are present in the human cervical carcinoma cell lines SiHa and CaSki. The single HPV-16 genome in the SiHa...

Heteroduplex analysis of molecular clones of the pathogenic Friend virus complex: Friend murine leukemia virus, Friend mink cell focus-forming virus, and the polycythemia- and anemia-inducing strains of Friend spleen focus-forming virus.

Gonda, M A, Kaminchick, J, Oliff, A, Menke, J, Nagashima, K, Scolnick, E M

The pathogenic Friend virus complex is of considerable interest in that, although members of this group are genetically related, they differ markedly in biochemical and biological properties....

Subcellular localization of glycoproteins encoded by the viral oncogene v-fms.

Anderson, S J, Gonda, M A, Rettenmier, C W, Sherr, C J

The McDonough strain of feline sarcoma virus encodes a polyprotein that is cotranslationally glycosylated and proteolytically cleaved to yield transforming glycoproteins specified by the viral...

The visna virus genome: evidence for a hypervariable site in the env gene and sequence homology among lentivirus envelope proteins.

Braun, M J, Clements, J E, Gonda, M A

The complete nucleotide sequence of the visna virus 1514 genome was determined. Our sequence confirms the relationship of visna virus and other lentiviruses to human immunodeficiency virus (HIV) both...

Molecular cloning of a family of retroviral sequences found in chimpanzee but not human DNA.

Bonner, T I, Birkenmeier, E H, Gonda, M A, Mark, G E, Searfoss, G H, Todaro, G J

A number of retrovirus-like sequences have been cloned from chimpanzee DNA which constitute the chimpanzee homologs of the endogenous colobus type C virus CPC-1. One of the clones contains a nearly...

Molecular cloning, genomic analysis, and biological properties of rat leukemia virus and the onc sequences of Rasheed rat sarcoma virus.

Gonda, M A, Young, H A, Elser, J E, Rasheed, S, Talmadge, C B, Nagashima, K, ...

Rasheed rat sarcoma virus (RaSV) has been shown to code for a protein of 29,000 Mr not present in replication-competent rat type C helper virus (RaLV)-infected cells. This protein is a fused gene...

Genome structure of mink cell focus-forming murine leukemia virus in epithelial mink lung cells transformed vitro by iododeoxyuridine-induced C3H/MuLV cells.

Rapp, U R, Birkenmeier, E, Bonner, T I, Gonda, M A, Gunnell, M

We characterized mink cell focus-forming murine leukemia viruses that were isolated from C3H/MCA-5 cells after induction with 5-iododeoxyuridine in culture. Mink lung epithelial cells malignantly...

Avian reticuloendotheliosis virus: characterization of the high-molecular-weight viral RNA in transforming and helper virus populations.

Gonda, M A, Rice, N R, Gilden, R V

Reticuloendotheliosis virus strain T (REV-T) is a type C retrovirus known to transform avian fibroblasts, spleen cells, and bone marrow cells and to produce virulent reticuloendotheliosis in young...

Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector.

Karacostas, V, Nagashima, K, Gonda, M A, Moss, B

Infectious retrovirus particles consist of a core structure containing RNA and gag-pol polypeptides surrounded by a lipid membrane studded with env proteins. A recombinant vaccinia virus was designed...

Sequence of MET protooncogene cDNA has features characteristic of the tyrosine kinase family of growth-factor receptors.

Park, M, Dean, M, Kaul, K, Braun, M J, Gonda, M A, Vande Woude, G

We isolated overlapping cDNA clones corresponding to the major MET protooncogene transcript. The cDNA nucleotide sequence contained an open reading frame of 1408 amino acids with features...

Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine.

Arthur, L O, Pyle, S W, Nara, P L, Bess, J W, Gonda, M A, Kelliher, J C, ...

The major envelope glycoprotein of a human immunodeficiency virus (HIV) has been purified and was utilized as a prototype vaccine in chimpanzees. The 120,000-dalton glycoprotein (gp120) was purified...

Absence of cytotoxic antibody to human immunodeficiency virus-infected cells in humans and its induction in animals after infection or immunization with purified envelope glycoprotein gp120.

Nara, P L, Robey, W G, Gonda, M A, Carter, S G, Fischinger, P J

The presence of antibody-dependent complement-mediated cytotoxicity (ACC) was assessed in humans and chimpanzees, which are capable of infection with human immunodeficiency virus isolate HTLV-IIIb,...

Analysis of two divergent rat genomic clones homologous to the transforming gene of Harvey murine sarcoma virus.

DeFeo, D, Gonda, M A, Young, H A, Chang, E H, Lowy, D R, Scolnick, E M, ...

Harvey murine sarcoma virus (Ha-MuSV) is a mouse-rat recombinant retrovirus that encodes a protein designated p21, required for virally induced transformation. Using a radiolabeled DNA fragment from...

Human T-cell lymphotropic virus type III shares sequence homology with a family of pathogenic lentiviruses.

Gonda, M A, Braun, M J, Clements, J E, Pyper, J M, Wong-Staal, F, Gallo, R C, ...

The etiologic agent of the acquired immune deficiency syndrome, human T-cell lymphotropic virus type III (HTLV-III), has recently been shown to morphologically resemble and share sequence homology...

Stability of RNA stem-loop structure and distribution of non-random structure in the human immunodeficiency virus (HIV-I).

Le, S Y, Chen, J H, Braun, M J, Gonda, M A, Maizel, J V

The stability of potential RNA stem-loop structures in human immunodeficiency virus isolates, HTLV-III and ARV, has been calculated, and the relevance to the local significant secondary structures in...

Genomes of evolutionarily divergent members of the human T-cell leukemia virus family (HTLV-I and HTLV-II) are highly conserved, especially in pX.

Shaw, G M, Gonda, M A, Flickinger, G H, Hahn, B H, Gallo, R C, Wong-Staal, F

Human T-cell leukemia virus (HTLV) is a family of related human T-lymphotropic retroviruses closely linked with certain human T-cell malignancies and associated with many cases of acquired...

Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses.

Chang, E H, Gonda, M A, Ellis, R W, Scolnick, E M, Lowy, D R

Harvey and Kirsten murine sarcoma viruses each encode a structurally and functionally related 21-kilodalton protein (p21), which is the transforming protein of each virus. Using probes from the...

Genomic diversity of the acquired immune deficiency syndrome virus HTLV-III: different viruses exhibit greatest divergence in their envelope genes.

Hahn, B H, Gonda, M A, Shaw, G M, Popovic, M, Hoxie, J A, Gallo, R C, ...

Converging lines of research have linked human T-cell lymphotropic virus type III (HTLV-III) to the pathogenesis of the acquired immune deficiency syndrome. A characteristic feature of this virus is...

Autoimmunity in chronic active Helicobacter hepatitis of mice. Serum antibodies and expression of heat shock protein 70 in liver.

Ward, J. M., Benveniste, R. E., Fox, C. H., Battles, J. K., Gonda, M. A., Tully, J. G.

Male A/JCr mice with naturally occurring Helicobacter hepaticus infection develop a progressive chronic active hepatitis and liver tumors, despite the presence of serum antibodies to Helicobacter...