Verotoxin-1 Binding to Human Monocytes Results in Induction of Cytokine Release (1995)
Monnens, L.A.H., Hinsbergh, V.W.M., Karmali, M.A., Verstraten, H.G.G., Vandenheuvel, L.P., Vansetten, P.A.
Bast, D J, Brunton, J L, Karmali, M A, Richardson, S E
The verotoxins (VT1 and VT2), produced by strains of enterohemorrhagic Escherichia coli, have been implicated in the pathogenesis of hemorrhagic colitis and the hemolytic uremic syndrome. To better...
Bielaszewska, M, Clarke, I, Karmali, M A, Petric, M
Rabbits challenged intravenously with Shiga toxin or with Escherichia coli verocytotoxin 1 or 2 (VT1 or VT2) are known to develop diarrhea, paralysis, and death, which can be prevented by...
Karmali, M A, De Grandis, S, Fleming, P C
Agar dilution antimicrobial susceptibility testing of Camphylobacter jejuni showed that erythromycin, clindamycin, nitrofurantoin, and gentamicin were the most active compounds, inhibiting 90% of the...
Transmissible plasmids from Campylobacter jejuni.
Taylor, D E, De Grandis, S A, Karmali, M A, Fleming, P C
Tetracycline resistance in clinical isolates of Campylobacter jejuni was shown to be plasmid mediated. Intra- and interspecies transfers to C. fetus subsp. fetus were demonstrated. The frequency of...
Reymond, D, Johnson, R P, Karmali, M A, Petric, M, Winkler, M, Johnson, S, ...
An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Escherichia coli O157 lipopolysaccharide (LPS) was developed with sera from 63 children with confirmed recent E. coli O157...
Heterogeneity in phenotypic and genotypic characteristics among strains of Hafnia alvei.
Ismaili, A, Bourke, B, De Azavedo, J C, Ratnam, S, Karmali, M A, Sherman, P M
Hafnia alvei is an emerging human pathogen associated with sporadic cases and outbreaks of diarrhea. Bangladeshi isolates of H. alvei possess the Escherichia coli attaching and effacing (eaeA) gene...
Reymond, D, Karmali, M A, Clarke, I, Winkler, M, Petric, M
A Western blot (immunoblot) assay (WBA) was developed to detect immunoglobulin G (IgG) antibodies against Escherichia coli Verocytotoxin 1 (VT1) by using a chemiluminescence detection system. The...
Experimental verocytotoxemia in rabbits.
Richardson, S E, Rotman, T A, Jay, V, Smith, C R, Becker, L E, Petric, M, ...
The clinicopathologic effects of intravenously administered purified verocytotoxin 1 (VT1; Shiga-like toxin 1) in 2-kg male rabbits was studied. The 50% lethal dose was 0.2 micrograms of protein per...
Utility of gram stain in evaluation of sputa from patients with cystic fibrosis.
Sadeghi, E, Matlow, A, MacLusky, I, Karmali, M A
The utility of sputum Gram stain in assessing salivary contamination and in predicting the presence of pathogens on the basis of morphology was investigated in 287 respiratory specimens from patients...
Karmali, M A, Petric, M, Winkler, M, Bielaszewska, M, Brunton, J, Van De Kar, N, ...
The frequency of Vero cytotoxin 1 (VT1)-neutralizing antibody (NAb) in serum specimens from 790 age-stratified (0 to 70 years) control individuals from Toronto was 61 of 790 (7.7%), with a peak of...
Campylobacter upsaliensis is the name which has been proposed for a new group of thermophilic campylobacter strains which differ from C. jejuni and C. coli in having a negative or weak catalase...
Karmali, M A, Petric, M, Lim, C, Cheung, R, Arbus, G S
High titers of Verotoxin (VT) were released from cell pellets of VT-producing Escherichia coli (VTEC; corresponding to E. coli strains producing "high" levels of Shiga-like toxin) after incubation in...
Karmali, M A, Simor, A E, Roscoe, M, Fleming, P C, Smith, S S, Lane, J
A blood-free, charcoal-based selective medium (CSM) consisting of a Columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32...
Petric, M, Karmali, M A, Arbus, G S, Roscoe, M, Louie, S, Cheung, R
Verocytotoxin (VT)-producing Escherichia coli is closely associated with hemorrhagic colitis and hemolytic uremic syndrome. The diagnosis of this infection requires the demonstration of VT activity...
Hii, J H, Gyles, C, Morooka, T, Karmali, M A, Clarke, R, De Grandis, S, ...
We and others have noted that there are serological differences between verotoxin 2 (VT2) (also known as Shiga-like toxin II) produced by Escherichia coli C600(933W) and the VT2 variant (VT2v)...
Infection caused by Yersinia enterocolitica serotype O:21.
Karmali, M A, Toma, S, Schiemann, D A, Ein, S H
Yersinia enterocolitica serotype O:21, biotype 3 (Wauters) was isolated from the appendix and stool of a 7-year-old girl. The same organism was later isolated in pure culture from pus from a...
Application of the Fortner principle to isolation of Campylobacter from stools.
A simple biological technique for reducing oxygen tension which does not necessitate the use of conventional anaerobic equipment is described. We successfully applied this method to the isolation of...
Bitzan, M, Richardson, S, Huang, C, Boyd, B, Petric, M, Karmali, M A
The interaction of verotoxins (VTs) with human erythrocytes (RBCs) in vitro was investigated, with particular reference to the role of P blood group glycolipids that are structurally related to the...
Karmali, M A, De Grandis, S, Fleming, P C
Agar dilution antimicrobial susceptibility testing showed that Campylobacter jejuni was significantly more resistant than Campylobacter fetus subsp. fetus (intestinalis) to cephalosporin C,...
Infection by verocytotoxin-producing Escherichia coli.
Verocytotoxin (VT)-producing Escherichia coli (VTEC) are a newly recognized group of enteric pathogens which are increasingly being recognized as common causes of diarrhea in some geographic...
Modified ammonia electrode method to investigate D-asparagine breakdown by Campylobacter strains.
Karmali, M A, Roscoe, M, Fleming, P C
An ammonia electrode method has been developed for investigating the deamination of amino acids by bacteria. It consists of incubating a standard inoculum of organisms in an amino acid solution and...
Sajjan, U S, Corey, M, Karmali, M A, Forstner, J F
Although not as prevalent as Pseudomonas aeruginosa, Pseudomonas cepacia is another opportunistic pathogen which colonizes the lungs of at least some patients with cystic fibrosis. A subgroup of...
Bast, D J, Brunton, J L, Karmali, M A, Richardson, S E
The verotoxins (VT1 and VT2), produced by strains of enterohemorrhagic Escherichia coli, have been implicated in the pathogenesis of hemorrhagic colitis and the hemolytic uremic syndrome. To better...
Bielaszewska, M, Clarke, I, Karmali, M A, Petric, M
Rabbits challenged intravenously with Shiga toxin or with Escherichia coli verocytotoxin 1 or 2 (VT1 or VT2) are known to develop diarrhea, paralysis, and death, which can be prevented by...
Karmali, M A, De Grandis, S, Fleming, P C
Agar dilution antimicrobial susceptibility testing of Camphylobacter jejuni showed that erythromycin, clindamycin, nitrofurantoin, and gentamicin were the most active compounds, inhibiting 90% of the...
Transmissible plasmids from Campylobacter jejuni.
Taylor, D E, De Grandis, S A, Karmali, M A, Fleming, P C
Tetracycline resistance in clinical isolates of Campylobacter jejuni was shown to be plasmid mediated. Intra- and interspecies transfers to C. fetus subsp. fetus were demonstrated. The frequency of...
Reymond, D, Johnson, R P, Karmali, M A, Petric, M, Winkler, M, Johnson, S, ...
An enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Escherichia coli O157 lipopolysaccharide (LPS) was developed with sera from 63 children with confirmed recent E. coli O157...
Heterogeneity in phenotypic and genotypic characteristics among strains of Hafnia alvei.
Ismaili, A, Bourke, B, De Azavedo, J C, Ratnam, S, Karmali, M A, Sherman, P M
Hafnia alvei is an emerging human pathogen associated with sporadic cases and outbreaks of diarrhea. Bangladeshi isolates of H. alvei possess the Escherichia coli attaching and effacing (eaeA) gene...
Reymond, D, Karmali, M A, Clarke, I, Winkler, M, Petric, M
A Western blot (immunoblot) assay (WBA) was developed to detect immunoglobulin G (IgG) antibodies against Escherichia coli Verocytotoxin 1 (VT1) by using a chemiluminescence detection system. The...
Experimental verocytotoxemia in rabbits.
Richardson, S E, Rotman, T A, Jay, V, Smith, C R, Becker, L E, Petric, M, ...
The clinicopathologic effects of intravenously administered purified verocytotoxin 1 (VT1; Shiga-like toxin 1) in 2-kg male rabbits was studied. The 50% lethal dose was 0.2 micrograms of protein per...
Utility of gram stain in evaluation of sputa from patients with cystic fibrosis.
Sadeghi, E, Matlow, A, MacLusky, I, Karmali, M A
The utility of sputum Gram stain in assessing salivary contamination and in predicting the presence of pathogens on the basis of morphology was investigated in 287 respiratory specimens from patients...
Karmali, M A, Petric, M, Winkler, M, Bielaszewska, M, Brunton, J, Van De Kar, N, ...
The frequency of Vero cytotoxin 1 (VT1)-neutralizing antibody (NAb) in serum specimens from 790 age-stratified (0 to 70 years) control individuals from Toronto was 61 of 790 (7.7%), with a peak of...
Campylobacter upsaliensis is the name which has been proposed for a new group of thermophilic campylobacter strains which differ from C. jejuni and C. coli in having a negative or weak catalase...
Karmali, M A, Petric, M, Lim, C, Cheung, R, Arbus, G S
High titers of Verotoxin (VT) were released from cell pellets of VT-producing Escherichia coli (VTEC; corresponding to E. coli strains producing "high" levels of Shiga-like toxin) after incubation in...
Karmali, M A, Simor, A E, Roscoe, M, Fleming, P C, Smith, S S, Lane, J
A blood-free, charcoal-based selective medium (CSM) consisting of a Columbia agar base, activated charcoal (4 g/liter), hematin (0.032 g/liter), sodium pyruvate (0.1 g/liter), cefoperazone (32...
Petric, M, Karmali, M A, Arbus, G S, Roscoe, M, Louie, S, Cheung, R
Verocytotoxin (VT)-producing Escherichia coli is closely associated with hemorrhagic colitis and hemolytic uremic syndrome. The diagnosis of this infection requires the demonstration of VT activity...
Hii, J H, Gyles, C, Morooka, T, Karmali, M A, Clarke, R, De Grandis, S, ...
We and others have noted that there are serological differences between verotoxin 2 (VT2) (also known as Shiga-like toxin II) produced by Escherichia coli C600(933W) and the VT2 variant (VT2v)...
Infection caused by Yersinia enterocolitica serotype O:21.
Karmali, M A, Toma, S, Schiemann, D A, Ein, S H
Yersinia enterocolitica serotype O:21, biotype 3 (Wauters) was isolated from the appendix and stool of a 7-year-old girl. The same organism was later isolated in pure culture from pus from a...
Application of the Fortner principle to isolation of Campylobacter from stools.
A simple biological technique for reducing oxygen tension which does not necessitate the use of conventional anaerobic equipment is described. We successfully applied this method to the isolation of...
Bitzan, M, Richardson, S, Huang, C, Boyd, B, Petric, M, Karmali, M A
The interaction of verotoxins (VTs) with human erythrocytes (RBCs) in vitro was investigated, with particular reference to the role of P blood group glycolipids that are structurally related to the...
Karmali, M A, De Grandis, S, Fleming, P C
Agar dilution antimicrobial susceptibility testing showed that Campylobacter jejuni was significantly more resistant than Campylobacter fetus subsp. fetus (intestinalis) to cephalosporin C,...
Infection by verocytotoxin-producing Escherichia coli.
Verocytotoxin (VT)-producing Escherichia coli (VTEC) are a newly recognized group of enteric pathogens which are increasingly being recognized as common causes of diarrhea in some geographic...
Modified ammonia electrode method to investigate D-asparagine breakdown by Campylobacter strains.
Karmali, M A, Roscoe, M, Fleming, P C
An ammonia electrode method has been developed for investigating the deamination of amino acids by bacteria. It consists of incubating a standard inoculum of organisms in an amino acid solution and...
Sajjan, U S, Corey, M, Karmali, M A, Forstner, J F
Although not as prevalent as Pseudomonas aeruginosa, Pseudomonas cepacia is another opportunistic pathogen which colonizes the lungs of at least some patients with cystic fibrosis. A subgroup of...
Campylobacter jejuni/coli has recently become recognized as a common bacterial cause of diarrhea. Infection can occur at any age. The usual incubation period of campylobacter enteritis is 2 to 5...