Giurgiovich, A.J., Diwan, B.A., Lee, K.B., Anderson, L.M., Rice, J.M., Poirier, M.C.
Cis-diamminedichloroplatinum (II) (cisplatin), given to pregnant rats at 5 mg/kg body weight (bw) is a transplacental carcinogen for fetal liver, kidney, nervous system and lung, resulting in tumor...
Kang, D.H., Rothman, N., Poirier, M.C., Greenberg, A., Hsu, C.H., Schwartz, B.S., ...
Biological markers of internal dose and macromolecular dose from PAHs provide a potential means of assessing environmental exposure to PAHs through inhalation, ingestion and percutaneous absorption....
Special article: Antineoplastic drug resistance and DNA repair (1991)
Burt, R. K., Poirier, M. C., Link, C. J., Bohr, V. A.
Important aspects of the DNA repair mechanisms in mammalian, and especially human, cells are reviewed. The DNA repair processes are essential in the maintenance of the integrity of the DNA and in the...
Rothman, N., Poirier, M.C., Baser, M.E., Hansen, J.A., Gentile, C., Bowman, E.D., ...
The effect of ingesting charcoal-broiled (CB) beef on polycydic aromatic hydrocarbon (PAH)-DNA adduct levels in nucleated peripheral white blood cells (WBCs) was examined in four healthy, non-smoking...
Santella, R.M., Weston, A., Perera, F.P., Trivers, G.T., Harris, C.C., Young, T.L., ...
An interlaboratory comparison of immunoassays using antisera elicited against benzo[a]pyrene-diol-epoxide-modified DNA (BPDE-I-DNA) was carried out resulting in standardization of antisera,...
Hsu, I.C., Poirier, M.C., Yuspa, S.H., Yolken, R.H., Harris, C.C.
USERIA is a modification of radioimmunoassay and enzyme-linked immunosorbent assay. When compared to these assays, USERIA is 60- and 10-fold, respectively, more sensitive for the detection of...
Huitfeldt, H S, Brandtzaeg, P, Poirier, M C
To investigate early cellular alterations in liver DNA during hepatocarcinogenesis, we have visualized replicating cells and analyzed their DNA adduct content in livers of rats continuously fed a...
Benzo[alpha]pyrene diol epoxide I binds to DNA at replication forks.
Paules, R S, Cordeiro-Stone, M, Mass, M J, Poirier, M C, Yuspa, S H, Kaufman, D G
The distribution of lesions in DNA caused by (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo [alpha]pyrene (B[alpha]P diol epoxide-I) was studied in synchronized...
Reed, E, Ozols, R F, Tarone, R, Yuspa, S H, Poirier, M C
Fifty-five ovarian cancer patients receiving platinum drug-based chemotherapy have been studied prospectively to determine the extent of formation of the bidentate intrastrand adducts of...
Poirier, M C, Lippard, S J, Zwelling, L A, Ushay, H M, Kerrigan, D, Thill, C C, ...
Rabbit antiserum elicited against calf thymus DNA modified to 4.4% (Pt drug/nucleotide ratio = 0.044) with the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP) contains antibodies specific...
Reed, E, Yuspa, S H, Zwelling, L A, Ozols, R F, Poirier, M C
The antitumor activity of cis-diamminedichloroplatinum II (cisplatin) is believed to be related to its covalent interaction with DNA where a major DNA binding product is an intrastrand N7-bidentate...
Specific quantification of mouse and human keratin proteins by radioimmunoassay.
Yuspa, S H, Poirier, M C, Harness, J R, Olsom, D R, Steinert, P M
Mouse epidermal keratin proteins were purified and labelled with 125I by chemical techniques. A radioimmunoassay method was established with a rabbit antibody elicited against the mouse keratin. This...
Human DNA adduct measurements: state of the art.
Human DNA adduct formation (covalent modification of DNA with chemical carcinogens) is a promising biomarker for elucidating the molecular epidemiology of cancer. Classes of compounds for which human...
DNA adducts as exposure biomarkers and indicators of cancer risk.
Quantitation of DNA adducts in human tissues has been achieved with highly sensitive techniques based on adduct radiolabeling, antisera specific for DNA adducts or modified DNA, and/or adduct...
DNA adduct measurements and tumor incidence during chronic carcinogen exposure in rodents.
In an attempt to elucidate the relationship between DNA adduct formation and tumorigenesis, DNA adducts were measured in the livers and bladders of mice during chronic exposure to several different...
Poirier, M C, Reed, E, Shamkhani, H, Tarone, R E, Gupta-Burt, S
Studies of platinum drug-DNA adduct formation in tissues of cancer patients have involved both atomic absorbance spectroscopy (AAS), which measures total DNA-bound platinum, and anti-cisplatin-DNA...
Detection of polycyclic aromatic hydrocarbon-DNA adducts in human lung.
Weston, A, Bowman, E D, Shields, P G, Trivers, G E, Poirier, M C, Santella, R M, ...
Synchronous fluorescence spectroscopy has been combined with immunoaffinity chromatography (IAC) and HPLC to detect polycyclic aromatic hydrocarbon (PAH)-DNA adducts and measure...
Biomarkers of carcinogen exposure and cancer risk in a coke plant.
Assennato, G, Ferri, G M, Tockman, M S, Poirier, M C, Schoket, B, Porro, A, ...
To evaluate the association between an indicator of carcinogen exposure (peripheral blood leukocyte DNA adducts of polycyclic aromatic hydrocarbons) and an early indicator of neoplastic...
Schoket, B, Phillips, D H, Poirier, M C, Vincze, I
32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were...
To elucidate the relationship between DNA adduct formation and tumorigenesis, a number of experiments have been conducted to measure DNA adducts in target tissues from experimental animals during...
Rothman, N, Poirier, M C, Haas, R A, Correa-Villasenor, A, Ford, P, Hansen, J A, ...
Previous investigations suggest that dietary sources of polycyclic aromatic hydrocarbons (PAHs) contribute to the PAH-DNA adduct load in peripheral white blood cells (WBCs). In the current study, we...
Culp, S J, Poirier, M C, Beland, F A
Dietary administration of the hepatocarcinogen 2-acetylaminofluorene (2-AAF) to rats results in the formation of a major hepatic DNA adduct, N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF). In...
Poirier, M C, Reed, E, Zwelling, L A, Ozols, R F, Litterst, C L, Yuspa, S H
cis-Diamminedichloroplatinum (II) (cis-DDP), the antitumor drug, is cytotoxic in vitro primarily by binding to DNA and disrupting its normal functions. We have studied cis-DDP modification of DNA in...
Huitfeldt, H S, Brandtzaeg, P, Poirier, M C
To investigate early cellular alterations in liver DNA during hepatocarcinogenesis, we have visualized replicating cells and analyzed their DNA adduct content in livers of rats continuously fed a...
Benzo[alpha]pyrene diol epoxide I binds to DNA at replication forks.
Paules, R S, Cordeiro-Stone, M, Mass, M J, Poirier, M C, Yuspa, S H, Kaufman, D G
The distribution of lesions in DNA caused by (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo [alpha]pyrene (B[alpha]P diol epoxide-I) was studied in synchronized...
Reed, E, Ozols, R F, Tarone, R, Yuspa, S H, Poirier, M C
Fifty-five ovarian cancer patients receiving platinum drug-based chemotherapy have been studied prospectively to determine the extent of formation of the bidentate intrastrand adducts of...
Poirier, M C, Lippard, S J, Zwelling, L A, Ushay, H M, Kerrigan, D, Thill, C C, ...
Rabbit antiserum elicited against calf thymus DNA modified to 4.4% (Pt drug/nucleotide ratio = 0.044) with the antitumor drug cis-diamminedichloroplatinum(II) (cis-DDP) contains antibodies specific...
Reed, E, Yuspa, S H, Zwelling, L A, Ozols, R F, Poirier, M C
The antitumor activity of cis-diamminedichloroplatinum II (cisplatin) is believed to be related to its covalent interaction with DNA where a major DNA binding product is an intrastrand N7-bidentate...
Specific quantification of mouse and human keratin proteins by radioimmunoassay.
Yuspa, S H, Poirier, M C, Harness, J R, Olsom, D R, Steinert, P M
Mouse epidermal keratin proteins were purified and labelled with 125I by chemical techniques. A radioimmunoassay method was established with a rabbit antibody elicited against the mouse keratin. This...
Human DNA adduct measurements: state of the art.
Human DNA adduct formation (covalent modification of DNA with chemical carcinogens) is a promising biomarker for elucidating the molecular epidemiology of cancer. Classes of compounds for which human...
DNA adducts as exposure biomarkers and indicators of cancer risk.
Quantitation of DNA adducts in human tissues has been achieved with highly sensitive techniques based on adduct radiolabeling, antisera specific for DNA adducts or modified DNA, and/or adduct...
DNA adduct measurements and tumor incidence during chronic carcinogen exposure in rodents.
In an attempt to elucidate the relationship between DNA adduct formation and tumorigenesis, DNA adducts were measured in the livers and bladders of mice during chronic exposure to several different...
Poirier, M C, Reed, E, Shamkhani, H, Tarone, R E, Gupta-Burt, S
Studies of platinum drug-DNA adduct formation in tissues of cancer patients have involved both atomic absorbance spectroscopy (AAS), which measures total DNA-bound platinum, and anti-cisplatin-DNA...
Detection of polycyclic aromatic hydrocarbon-DNA adducts in human lung.
Weston, A, Bowman, E D, Shields, P G, Trivers, G E, Poirier, M C, Santella, R M, ...
Synchronous fluorescence spectroscopy has been combined with immunoaffinity chromatography (IAC) and HPLC to detect polycyclic aromatic hydrocarbon (PAH)-DNA adducts and measure...
Biomarkers of carcinogen exposure and cancer risk in a coke plant.
Assennato, G, Ferri, G M, Tockman, M S, Poirier, M C, Schoket, B, Porro, A, ...
To evaluate the association between an indicator of carcinogen exposure (peripheral blood leukocyte DNA adducts of polycyclic aromatic hydrocarbons) and an early indicator of neoplastic...
Schoket, B, Phillips, D H, Poirier, M C, Vincze, I
32P-Postlabeling analysis and enzyme-linked immunosorbent assay (ELISA) have been used to detect DNA adducts in peripheral blood lymphocytes from primary aluminum production plant workers who were...
To elucidate the relationship between DNA adduct formation and tumorigenesis, a number of experiments have been conducted to measure DNA adducts in target tissues from experimental animals during...
Rothman, N, Poirier, M C, Haas, R A, Correa-Villasenor, A, Ford, P, Hansen, J A, ...
Previous investigations suggest that dietary sources of polycyclic aromatic hydrocarbons (PAHs) contribute to the PAH-DNA adduct load in peripheral white blood cells (WBCs). In the current study, we...
Culp, S J, Poirier, M C, Beland, F A
Dietary administration of the hepatocarcinogen 2-acetylaminofluorene (2-AAF) to rats results in the formation of a major hepatic DNA adduct, N-(deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF). In...
Poirier, M C, Reed, E, Zwelling, L A, Ozols, R F, Litterst, C L, Yuspa, S H
cis-Diamminedichloroplatinum (II) (cis-DDP), the antitumor drug, is cytotoxic in vitro primarily by binding to DNA and disrupting its normal functions. We have studied cis-DDP modification of DNA in...