M. D. Houslay

A scanning peptide array approach uncovers association sites within the JNK/βarrestin signalling complex (2009)

Li, X., MacLeod, R., Dunlop, A.J., Edwards, H.V., Advant, M., Gibson, L.C.D., ...

βarrestins are molecular scaffolds that can bring together three-component mitogen-activated protein kinase signalling modules to promote signal compartmentalisation. We use peptide array...

Arrestin times for developing antipsychotics and {beta}-blockers (2009)

Houslay, M.D.

Heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) are the largest group of structurally related proteins encoded by the human genome. As signal effectors and...

Phosphorylation of RACK1 on tyrosine 52 by c-Abl is required for IGF-I-mediated regulation of focal adhesion kinase (FAK) (2009)

Kiely, P.A., Baillie, G.S., Barrett, R., Buckley, D.A., Adams, D.R., Houslay, M.D., ...

Focal Adhesion Kinase (FAK) activity is controlled by growth factors and adhesion signals in tumor cells. The scaffolding protein RACK1 (Receptor for Activated C Kinases) integrates Insulin-like...

Mdm2 directs the ubiquitination of {Beta}-arrestin-sequestered cAMP phosphodiesterase-4D5 (2009)

Li, X., Baillie, G.S., Houslay, M.D.

{beta}arrestin plays a key role in regulating {beta}2-adrenoceptor signalling by interdicting activation of adenylyl cyclase and selectively sequestering cAMP phosphodiesterase-4D5 (PDE4D5) for...

The role of the PDE4D cAMP phosphodiesterase in the regulation of glucagon-like peptide-1 release (2009)

Ong, W.K., Gribble, F.M., Reimann, F., Lynch, M.J., Houslay, M.D., Baillie, G.S., ...

Background and purpose: Increases in intracellular cyclic AMP (cAMP) augment the release/secretion of glucagon-like peptide-1 (GLP-1). As cAMP is hydrolysed by cAMP phosphodiesterases (PDEs), we...

Disrupting specific PDZ domain-mediated interactions for therapeutic benefit (2009)

Houslay, M.D.

The past two decades have seen an immense increase in our appreciation of the vast range of signalling processes and supporting machinery that occur in cells. Pivotal to this is the notion of signal...

Phosphorylation of RACK1 on tyrosine 52 by c-Abl is required for insulin-like growth factor i-mediated regulation of focal adhesion kinase (2009)

Kiely, P.A., Baillie, G.S., Barrett, R., Buckley, D.A., Adams, D.R., Houslay, M.D., ...

Focal Adhesion Kinase (FAK) activity is controlled by growth factors and adhesion signals in tumor cells. The scaffolding protein RACK1 (receptor for activated C kinases) integrates insulin-like...

Ndel1 alters its conformation by sequestering cAMP-specific phosphodiesterase-4D3 (PDE4D3) in a manner that is dynamically regulated through Protein Kinase A (PKA) (2008)

Collins, D.M., Murdoch, H., Dunlop, A.J., Charych, E., Baillie, G.S., Wang, Q., ...

The involvement of the Nuclear element distribution-like (Ndel1; Nudel) protein in the recruitment of the dynein complex is critical for neurodevelopment and potentially important for neuronal...

Tyrosine 302 in RACK1 is essential for insulin-like growth factor-I-mediated competitive binding of PP2A and {beta}1 integrin and for tumor cell proliferation and migration (2008)

Kiely, P.A., Baillie, G.S., Lynch, M.J., Houslay, M.D., O'Connor, R.

Insulin-like growth factor (IGF)-I regulates a mutually exclusive interaction of PP2A and {beta}1 integrin with the WD repeat scaffolding protein RACK1. This interaction is required for the...

Investigation of the alkenyldiarylmethane non-nucleoside reverse transcriptase inhibitors as potential cAMP phosphodiesterase-4B2 inhibitors (2008)

Cullen, M.D., Cheung, Y.F., Houslay, M.D., Hartman, T.L., Watson, K.M., Buckheit, R.W., ...

The alkenyldiarylmethanes (ADAMs) are currently being investigated as non- nucleoside HIV- 1 reverse transcriptase inhibitors (NNRTIs) of potential value in the treatment of HIV infection and AIDS....

Constitutive activation of the G-protein subunit Gαs within forebrain neurons causes PKA-dependent alterations in fear conditioning and cortical arc mRNA expression (2008)

Kelly, M.P., Cheung, Y.F., Favilla, C., Siegel, S.J., Kanes, S.J., Houslay, M.D., ...

Memory formation requires cAMP signaling; thus, this cascade has been of great interest in the search for cognitive enhancers. Given that medications are administered long-term, we determined the...

Mutations of [Beta]-arrestin 2 that limit self-association also interfere with interactions with the [Beta subscript2]-adrenoceptor and the ERK1/2 MAP kinases: Implications for [Beta subscript2]-adrenoceptor-signalling via the ERK1/2 MAP kinases (2008)

Xu, T-R., Baillie, G.S., Bhari, N., Houslay, T.M., Pitt, A.M., Adams, D.R., ...

Fluorescence resonance energy transfer (FRET) and co-immunoprecipitation studies confirmed the capacity of [Beta]-arrestin 2 to self-associate. Amino acids potentially involved in direct...

Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase by prenylation and interaction with a prenyl-binding protein (2008)

Day, J.R., Cleghon, V., Houslay, M.D., Davies, S.A.

Post-translational modification by isoprenylation is a pivotal process for the correct functioning of many signalling proteins. The Drosophila melanogaster cGMP-PDE (cGMP-specific phosphodiesterase)...

Protein kinase A type I and type II define distinct intracellular signaling compartments (2008)

Di Benedetto, G., Zoccarato, A., Lissandron, V., Terrin, A., Li, X., Houslay, M.D., ...

Protein kinase (PK)A is a key regulatory enzyme that, on activation by cAMP, modulates a wide variety of cellular functions. PKA isoforms type I and type II possess different structural features and...

Constitutive activation of the G-protein subunit G{alpha}s within forebrain neurons causes PKA-dependent alterations in fear conditioning and cortical Arc mRNA expression (2008)

Kelly, M.P., Cheung, Y.F., Favilla, C., Siegel, S.J., Kanes, S.J., Houslay, M.D., ...

Memory formation requires cAMP signaling; thus, this cascade has been of great interest in the search for cognitive enhancers. Given that medications are administered long-term, we determined the...

EPAC and PKA allow cAMP dual control over DNA-PK nuclear translocation (2008)

Huston, E., Lynch, M.J., Mohamed, A., Collins, D.M., Hill, E.V., McLeod, R., ...

We identify a compartmentalized signaling system that identifies a functional role for the GTP exchange factor, exchange protein activated by cAMP (EPAC) coupled to Rap2 in the nucleus. In this...

Isoform-selective susceptibility of DISC1/phosphodiesterase-4 complexes to dissociation by elevated intracellular cAMP levels (2007)

Murdoch, H., Mackie, S., Collins, D.M., Hill, E.V., Bolger, G.B., Klussmann, E., ...

Disrupted-in-schizophrenia 1 (DISC1) is a genetic susceptibility factor for schizophrenia and related severe psychiatric conditions. DISC1 is a multifunctional scaffold protein that is able to...

p75 neurotrophin receptor regulates tissue fibrosis through inhibition of plasminogen activation via a PDE4/cAMP/PKA pathway (2007)

Sachs, B.D., Baillie, G.S., McCall, J.R., Passino, M.A., Schachtrup, C., Wallace, D.A., ...

Clearance of fibrin through proteolytic degradation is a critical step of matrix remodeling that contributes to tissue repair in a variety of pathological conditions, such as stroke, atherosclerosis,...

Mapping binding sites for the PDE4D5 cAMP-specific phosphodiesterase to the N- and C-domains of beta-arrestin using spot-immobilized peptide arrays (2007)

Baillie, G.S., Adams, D.R., Bhari, N., Houslay, T.M., Vadrevu, S., Meng, D., ...

Beta2-ARs (beta2-adrenoceptors) become desensitized rapidly upon recruitment of cytosolic beta-arrestin. PDE4D5 (family 4 cAMP-specific phosphodiesterase, subfamily D, isoform 5) can be recruited in...

A novel role for a Drosophila homologue of cGMP-specific phosphodiesterase in the active transport of cGMP (2006)

Day, J.P., Houslay, M.D., Davies, S-A.

cGMP was first discovered in urine, demonstrating that kidney cells extrude this cyclic nucleotide. Drosophila Malpighian tubules provide a model renal system in which a homologue of mammalian PDE...

Scanning peptide array analysis identify overlapping binding sites for the signaling scaffold proteins, beta-arestin and RACK1 in the cAMP-specific phosphodiesterase, PDE4D5 (2006)

Bolger, G.B., Baillie, G.S., Li, X., Lynch, M.J., Herzyk, P., Mohamed, A., ...

The cAMP-specific phosphodiesterase PDE4D5 can interact with the signalling scaffold proteins RACK (receptors for activated C-kinase) 1 and β-arrestin. Two-hybrid and co-immunoprecipitation...

Molecular basis of insulin receptor function (1989)

Houslay, M D, Siddle, K

Insulin regulates a wide spectrum of metabolic processes in a variety of tissues1. In addition, insulin is a potent growth factor, promoting normal cell growth and division in cultured cells, though...