Alldread, R M, Halsall, D M, Clarke, A R, Sundaram, T K, Atkinson, T, Scawen, M D, ...
The nucleotide-binding fold of many NAD(+)-dependent dehydrogenases contains a conserved acidic amino acid residue which hydrogen-bonds with the 2'- and 3'-hydroxy groups of the adenine-ribose of the...
Moola, Z B, Scawen, M D, Atkinson, T, Nicholls, D J
This study shows that the antigenicity of Erwinia chrysanthemi L-asparaginase can be reduced by site-directed mutagenesis. Ten B-cell epitopes of the enzyme were identified using synthetic...
Nobbs, T J, Cortés, A, Gelpi, J L, Holbrook, J J, Atkinson, T, Scawen, M D, ...
The X-ray structure of lactate dehydrogenase (LDH) shows the side-chain carboxylate group of Asp-143 to be buried in the hydrophobic interior of the enzyme, where it makes hydrogen-bonding...
Goward, C R, Hartwell, R, Atkinson, T, Scawen, M D
Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation of extraneous material at pH 5.5,...
Goward, C R, Scawen, M D, Atkinson, T
Glucokinase from Bacillus stearothermophilus was irreversibly inactivated by the reactive dichlorotriazinyl dye Procion Blue MX-3G at pH 8.0. The enzyme was protected from inactivation by the...
Scawen, M D, Darbyshire, J, Harvey, M J, Atkinson, T
3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two...
The amino acid sequence of plastocyanin from spinach. (Spinacia oleracea L.).
Scawen, M D, Ramshaw, J A, Boulter, D
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The...
Wynne, S A, Nicholls, D J, Scawen, M D, Sundaram, T K
The gene encoding the tetrameric malate dehydrogenase (MDH) in a thermophilic Bacillus species (BI) has been cloned in an Escherichia coli plasmid. The nucleotide sequence of the gene, the first to...
Cadmium-binding proteins in Pseudomonas putida: pseudothioneins.
Higham, D P, Sadler, P J, Scawen, M D
Pseudomonas putida adapted to growth in 3 mM cadmium. The resistance mechanism involved complexation of cadmium in polyphosphate granules, changes in the structure of the cell membrane and induction...
Alldread, R M, Halsall, D M, Clarke, A R, Sundaram, T K, Atkinson, T, Scawen, M D, ...
The nucleotide-binding fold of many NAD(+)-dependent dehydrogenases contains a conserved acidic amino acid residue which hydrogen-bonds with the 2'- and 3'-hydroxy groups of the adenine-ribose of the...
Moola, Z B, Scawen, M D, Atkinson, T, Nicholls, D J
This study shows that the antigenicity of Erwinia chrysanthemi L-asparaginase can be reduced by site-directed mutagenesis. Ten B-cell epitopes of the enzyme were identified using synthetic...
Nobbs, T J, Cortés, A, Gelpi, J L, Holbrook, J J, Atkinson, T, Scawen, M D, ...
The X-ray structure of lactate dehydrogenase (LDH) shows the side-chain carboxylate group of Asp-143 to be buried in the hydrophobic interior of the enzyme, where it makes hydrogen-bonding...
Goward, C R, Hartwell, R, Atkinson, T, Scawen, M D
Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation of extraneous material at pH 5.5,...
Goward, C R, Scawen, M D, Atkinson, T
Glucokinase from Bacillus stearothermophilus was irreversibly inactivated by the reactive dichlorotriazinyl dye Procion Blue MX-3G at pH 8.0. The enzyme was protected from inactivation by the...
Scawen, M D, Darbyshire, J, Harvey, M J, Atkinson, T
3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity on a large scale involving only two sequential affinity-chromatography steps on two...
The amino acid sequence of plastocyanin from spinach. (Spinacia oleracea L.).
Scawen, M D, Ramshaw, J A, Boulter, D
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The...
Wynne, S A, Nicholls, D J, Scawen, M D, Sundaram, T K
The gene encoding the tetrameric malate dehydrogenase (MDH) in a thermophilic Bacillus species (BI) has been cloned in an Escherichia coli plasmid. The nucleotide sequence of the gene, the first to...
Cadmium-binding proteins in Pseudomonas putida: pseudothioneins.
Higham, D P, Sadler, P J, Scawen, M D
Pseudomonas putida adapted to growth in 3 mM cadmium. The resistance mechanism involved complexation of cadmium in polyphosphate granules, changes in the structure of the cell membrane and induction...