Krojer, T., Pangerl, K., Kurt, J., Sawa, J., Stingl, C., Mechtler, K., ...
The HtrA family of proteases: Implications for protein composition and cell fate (2002)
Clausen,T., Southan,C., Ehrmann,M.
Cells precisely monitor the concentration and functionality of each protein for optimal performance. Protein quality control involves molecular chaperones, folding catalysts, and proteases that are...
Crystal structure of DegP (HtrA) reveals a new protease- chaperone machine (2002)
Krojer,T., Garrido-Franco,M., Huber,R., Ehrmann,M., Clausen,T.
Molecular chaperones and proteases monitor the folded state of other proteins. In addition to recognizing non-native conformations, these quality control factors distinguish substrates that can be...
The HtrA family of proteases: Implications for protein composition and cell fate (2002)
Clausen, T., Southan, C., Ehrmann, M.
Cells precisely monitor the concentration and functionality of each protein for optimal performance. Protein quality control involves molecular chaperones, folding catalysts, and proteases that are...
Crystal structure of DegP (HtrA) reveals a new protease- chaperone machine (2002)
Krojer, T., Garrido-Franco, M., Huber, R., Ehrmann, M., Clausen, T.
Molecular chaperones and proteases monitor the folded state of other proteins. In addition to recognizing non-native conformations, these quality control factors distinguish substrates that can be...
Firm Size and Monetary Policy Transmission - Evidence from German Business Survey Data.
Using business survey data on German manufacturing firms, this paper provides tests for hypotheses that predict distributrional effects in the transmission mechanism of monetary policy. Effects of...
Genetic analysis of membrane protein topology by a sandwich gene fusion approach.
Ehrmann, M, Boyd, D, Beckwith, J
We describe a cloning vector that allows the construction of phoA sandwich fusions in which mature alkaline phosphatase is inserted into target proteins. In contrast to previous fusions obtained...
Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.
Ehrle, R, Pick, C, Ulrich, R, Hofmann, E, Ehrmann, M
Oligonucleotide mutagenesis was used to isolate mutations in membrane-spanning segments 6, 7, and 8 of MalF. MalF is a cytoplasmic membrane component of the binding protein-dependent maltose...
Site-specific proteolysis of the Escherichia coli SecA protein in vivo.
A seven-amino-acid cleavage site specific for tobacco etch virus (TEV) protease was introduced into SecA at two separate positions after amino acids 195 and 252. Chromosomal wild-type secA was...
Characterization of a cytoplasmic trehalase of Escherichia coli.
Horlacher, R, Uhland, K, Klein, W, Ehrmann, M, Boos, W
Escherichia coli can synthesize trehalose in response to osmotic stress and is able to utilize trehalose as a carbon source. The pathway of trehalose utilization is different at low and high...
Requirements for translocation of periplasmic domains in polytopic membrane proteins.
Uhland, K, Ehrle, R, Zander, T, Ehrmann, M
Periplasmic domains of cytoplasmic membrane proteins require export signals for proper translocation. These signals were studied by using a MalF-alkaline phosphatase fusion in a genetic selection...
Reidl, J, Römisch, K, Ehrmann, M, Boos, W
The maltose regulon of Escherichia coli comprises several operons that are under common regulatory control of the MalT activator protein. Five mal genes, organized in two divergent operons, code for...
Ehrmann, M, Boos, W, Ormseth, E, Schweizer, H, Larson, T J
The glpTQ operon and the glpA and glpB genes are located adjacent to one another near min 49 of the linkage map of Escherichia coli K-12. The positions and directions of transcription of the glpA and...
Identification of endogenous inducers of the mal regulon in Escherichia coli.
The expression of the maltose regulon in Escherichia coli is induced when maltose or maltodextrins are present in the growth medium. Mutations in malK, which codes for a component of the transport...
Osmoregulation of the maltose regulon in Escherichia coli.
The maltose regulon consists of four operons that direct the synthesis of proteins required for the transport and metabolism of maltose and maltodextrins. Expression of the mal genes is induced by...
Whitley, P, Zander, T, Ehrmann, M, Haardt, M, Bremer, E, Von Heijne, G
The ProW protein, located in the inner membrane of Escherichia coli, has a very unusual topology with a 100-residue-long N-terminal tail protruding into the periplasmic space. We have studied the...
Prinz, W A, Spiess, C, Ehrmann, M, Schierle, C, Beckwith, J
Most extracytoplasmic proteins are synthesized with an N-terminal signal sequence that targets them to the export apparatus. Escherichia coli prlA mutants (altered in the secY gene) are able to...
Decoding signals for membrane protein assembly using alkaline phosphatase fusions.
McGovern, K, Ehrmann, M, Beckwith, J
We have used genetic methods to investigate the role of the different domains of a bacterial cytoplasmic membrane protein, MalF, in determining its topology. This was done by analyzing the effects of...
Genetic analysis of membrane protein topology by a sandwich gene fusion approach.
Ehrmann, M, Boyd, D, Beckwith, J
We describe a cloning vector that allows the construction of phoA sandwich fusions in which mature alkaline phosphatase is inserted into target proteins. In contrast to previous fusions obtained...
Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.
Ehrle, R, Pick, C, Ulrich, R, Hofmann, E, Ehrmann, M
Oligonucleotide mutagenesis was used to isolate mutations in membrane-spanning segments 6, 7, and 8 of MalF. MalF is a cytoplasmic membrane component of the binding protein-dependent maltose...
Site-specific proteolysis of the Escherichia coli SecA protein in vivo.
A seven-amino-acid cleavage site specific for tobacco etch virus (TEV) protease was introduced into SecA at two separate positions after amino acids 195 and 252. Chromosomal wild-type secA was...
Characterization of a cytoplasmic trehalase of Escherichia coli.
Horlacher, R, Uhland, K, Klein, W, Ehrmann, M, Boos, W
Escherichia coli can synthesize trehalose in response to osmotic stress and is able to utilize trehalose as a carbon source. The pathway of trehalose utilization is different at low and high...
Requirements for translocation of periplasmic domains in polytopic membrane proteins.
Uhland, K, Ehrle, R, Zander, T, Ehrmann, M
Periplasmic domains of cytoplasmic membrane proteins require export signals for proper translocation. These signals were studied by using a MalF-alkaline phosphatase fusion in a genetic selection...
Reidl, J, Römisch, K, Ehrmann, M, Boos, W
The maltose regulon of Escherichia coli comprises several operons that are under common regulatory control of the MalT activator protein. Five mal genes, organized in two divergent operons, code for...
Ehrmann, M, Boos, W, Ormseth, E, Schweizer, H, Larson, T J
The glpTQ operon and the glpA and glpB genes are located adjacent to one another near min 49 of the linkage map of Escherichia coli K-12. The positions and directions of transcription of the glpA and...
Identification of endogenous inducers of the mal regulon in Escherichia coli.
The expression of the maltose regulon in Escherichia coli is induced when maltose or maltodextrins are present in the growth medium. Mutations in malK, which codes for a component of the transport...
Osmoregulation of the maltose regulon in Escherichia coli.
The maltose regulon consists of four operons that direct the synthesis of proteins required for the transport and metabolism of maltose and maltodextrins. Expression of the mal genes is induced by...
Whitley, P, Zander, T, Ehrmann, M, Haardt, M, Bremer, E, Von Heijne, G
The ProW protein, located in the inner membrane of Escherichia coli, has a very unusual topology with a 100-residue-long N-terminal tail protruding into the periplasmic space. We have studied the...
Prinz, W A, Spiess, C, Ehrmann, M, Schierle, C, Beckwith, J
Most extracytoplasmic proteins are synthesized with an N-terminal signal sequence that targets them to the export apparatus. Escherichia coli prlA mutants (altered in the secY gene) are able to...
Decoding signals for membrane protein assembly using alkaline phosphatase fusions.
McGovern, K, Ehrmann, M, Beckwith, J
We have used genetic methods to investigate the role of the different domains of a bacterial cytoplasmic membrane protein, MalF, in determining its topology. This was done by analyzing the effects of...
Uncertain Potential Output: Implications for Monetary Policy.
This paper uses a small, calibrated forward-looking model of the euro-area economy to investigate the implications of incomplete information about potential output for the conduct and the design of...
Interbank Lending and Monetary Policy Transmission: Evidence for Germany.
This paper presents empirical evidence on the behaviour of interbank lending in Germany after a monetary policy impulse. Our VAR analysis shows that following a monetary contraction, the banking...
Regime-dependent Impulse Response Functions in a Markov-switching Vector Autoregression Model.
Ehrmann, M., Ellison, M., Valla, N.
In this paper we introduce identifying restrictions into a Markov-switching vector autoregression model. We define a separate set of impulse responses for each Markov regime to show how fundamental...
Firm Size and Monetary Policy Transmission - Evidence from German Business Survey Data.
Using business survey data on German manufacturing firms, this paper provides tests for hypotheses that predict distributrional effects in the transmission mechanism of monetary policy. Effects of...