Wei Zhang, Mark J. Ratain, M. Eileen Dolan
The exploration of quantitative variation in complex traits such as gene expression and drug response in human populations has become one of the major priorities for medical genetics. The...
Population-specific GSTM1 copy number variation (2009)
Huang, R. Stephanie, Chen, Peixian, Wisel, Steve, Duan, Shiwei, Zhang, Wei, Cook, Edwin H., ...
As one of the major glutathione conjugation enzymes, GSTM1 detoxifies a number of drugs and xenobiotics. Its expression and activity have been shown to correlate both with cancer risks and drug...
Mouse models of human AML accurately predict chemotherapy response (2009)
Zuber, Johannes, Radtke, Ina, Pardee, Timothy S., Zhao, Zhen, Rappaport, Amy R., Luo, Weijun, ...
The genetic heterogeneity of cancer influences the trajectory of tumor progression and may underlie clinical variation in therapy response. To model such heterogeneity, we produced genetically and...
On the challenges of the HapMap resource (2008)
The International HapMap Project provides a key resource of genotypic data on human lymphoblastoid cell lines derived from four major world populations of European, African, Chinese and Japanese...
Wei Zhang, Mark J. Ratain, M. Eileen Dolan
The exploration of quantitative variation in complex traits such as gene expression and drug response in human populations has become one of the major priorities for medical genetics. The...
HapMap filter 1.0: A tool to preprocess the HapMap genotypic data for association studies (2008)
Wei Zhang, Shiwei Duan, M. Eileen Dolan
The International HapMap Project provides a resource of genotypic data on single nucleotide polymorphisms (SNPs), which can be used in various association studies to identify the genetic determinants...
Though debates exist on the early human evolutionary models such as the “Out of Africa” theory, which hypothesizes that modern humans migrated from Africa to Europe about 50,000 to 100,000 years...
Shiwei Duan, Wei Zhang, Wasim Kamal Bleibel, Nancy Jean Cox, M. Eileen Dolan
The Affymetrix GeneChip® Human Exon 1.0 ST array (exon array) is designed to measure both gene-level and exon-level expression in human samples. This exon array contains ~1.4 million probesets...
Nagasubramanian, Ramamoorthy, Hansen, Ryan J., Delaney, Shannon M., Cherian, Mathew M., Samson, Leona D., Kogan, Scott C., ...
O6-methylguanine DNA methyltransferase (MGMT) deficiency is associated with an increased susceptibility to alkylating agent toxicity. To understand the contribution of MGMT in protecting against...
Hansen, Ryan J., Nagasubramanian, Ramamoorthy, Delaney, Shannon M., Samson, Leona D., Dolan, M.Eileen
The DNA repair protein O6-methylguanine-DNA methyltransferase (MGMT) protects from toxicity and mutations incurred following alkylating agents by removing O6-alkylguanine lesions. Using Mgmt−/−...
Wali, Ramesh K., Skarosi, Susan, Hart, John, Zhang, Yingchun, Dolan, M.eileen, Moschel, Robert C., ...
Azoxymethane (AOM) causes O6-methylguanine adduct formation which leads to G→A transitions. Their repair is carried out by O6-methylguanine-DNA methyltransferase (MGMT). To evaluate the importance...
Bishop, Ronald E., Dunn, Linda L., Pauly, Gary T., Dolan, M. Eileen, Moschel, Robert C.
The role of rat O6-alkylguanine-DNA alkyltransferase (AGT) in modulating mutagenesis by O6-substituted guanines in the rat H-ras gene was examined. Rat4 cells were transfected with vectors carrying...
Jacoby, Russell F., Bolt, Merry J.G., Dolan, M.Eileen, Otto, Glen, Dudeja, Pradeep, Sitrin, Michael D., ...
Prior studies from our laboratory have demonstrated that K-ras G to A mutations were detectable in a high percentage of carcinomas which developed in the colons of animals treated with the known...
Enhancement of Nitrosourea Activity in Medulloblastoma and Glioblastoma Multiforme (1992)
Friedman, Henry S., Dolan, M. Eileen, Moschel, Robert C., Pegg, Anthony E., Felker, G. Michael, Rich, Jeremy, ...
Background: Although chemotherapy offers promise of increased survival for children with medulloblastoma and glioblastoma multiforme, drug resistance occurs frequently, resulting in tumor progression...
Pegg, Anthony E., Wiest, Laurie, Mummert, Christine, Dolan, M.Eileen
Antisera were raised in rabbits to three peptides which correspond to sequences of amino acids present in human O6-alkylguanine-DNA alkyltransferase (residues 1–11, 8–20 and 197–207). These...
Pegg, Anthony E., Wiest, Laurie, Mummert, Christine, Stine, Linda, Moschel, Robert C., Dolan, M.Eileen
Antisera raised in rabbits to three peptides corresponding to amino acid sequences found in human O6 -alkylguanine-DNA alkyltransferase were used to study the fate of the alkyltransferase protein in...
Dolan, M. Eileen, Pegg, Anthony E., Dumenco, Luba L., Moschel, Robert C., Gerson, Stanton L.
The inactivation of human and Escherichia coli O6-alkylguanine-DNA alkyltransferase by O6-methylguanine and O6-benzylguanine was compared. When HT29 cell extracts or E.coli Ada protein were incubated...
Dolan, M.Eileen, Norbeck, Lauri, Clyde, Christopher, Hora, Nancy K., Erickson, Leonard C., Pegg, Anthony E.
Chinese hamster ovary cells (CHO) were co-transfected with pSV2neo and sheared DNA from either a human cell line (HT29) expressing high levels of O6-alkylguanlne-DNA alkyltransferase (AGT) or from a...
Belinsky, Steven A., Dolan, M.Eileen, White, Catherine M., Maronpot, Robert R., Pegg, Anthony E., Anderson, Marshall W.
Previous studies have demonstrated that cell specificity exists for the alkylation of DNA from lung cells following treatment of rats with the tobacco specific carcinogen...
Sequence specificity of guanine alkylation and repair (1988)
Dolan, M.Eileen, Oplinger, Michele, Pegg, Anthony E.
The sequence selectivity of methylation at the O6 and N7 position of guanine by N-methyl-N'-nitrosourea (MNU) and the rate of removal of O6-methylguanine by O6 alkylguanine-DNA alkyltransferase (AGT)...
Dolan, M.Eileen, Pegg, Anthony E.
Calf thymus DNA was methylated by reaction with N-[3H]-methyl-N-nitrosourea and the content of O6-methyldeoxy-guanosine, 3-methylthymidine and O4-methylthymidine was determined. It was found that...
Domoradzki, Jeanne, Pegg, Anthony E., Dolan, M.Eileen, Maher, Veronica M., McCormick, J.Justin
We showed previously that resistance of a series of human fibroblast cell lines to the cytotoxic and mutagenic effects of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) is highly correlated with their...
Morimoto, Kazushige, Dolan, M. Eileen, Scicchitano, David, Pegg, Anthony E.
DNA substrates containing O6-n-butylguanine, O6-iso-butylguanine, O6-n-propylguanine and O6-iso-propylguanine were prepared by reaction of calf thymus DNA with the appropriate N-alkyl-N-nitrosourea....
Domoradzki, Jeanne, Pegg, Anthony E., Dolan, M. Eileen, Maher, Veronica M., McCormick, J. Justin
Cells from Gardner's syndrome (GS) and familial polyposis coli (FP) patients, persons with a hereditary predisposition to colon cancer, were compared to those of normal persons for sensitivity to the...
Huang, R. Stephanie, Duan, Shiwei, Bleibel, Wasim K., Kistner, Emily O., Zhang, Wei, Clark, Tyson A., ...
Large interindividual variance has been observed in sensitivity to drugs. To comprehensively decipher the genetic contribution to these variations in drug susceptibility, we present a genome-wide...
On the challenges of the HapMap resource
The International HapMap Project provides a key resource of genotypic data on human lymphoblastoid cell lines derived from four major world populations of European, African, Chinese and Japanese...
HapMap filter 1.0: A tool to preprocess the HapMap genotypic data for association studies
Zhang, Wei, Duan, Shiwei, Dolan, M Eileen
The International HapMap Project provides a resource of genotypic data on single nucleotide polymorphisms (SNPs), which can be used in various association studies to identify the genetic determinants...
Duan, Shiwei, Zhang, Wei, Bleibel, Wasim Kamel, Cox, Nancy Jean, Dolan, M Eileen
The Affymetrix GeneChip® Human Exon 1.0 ST array (exon array) is designed to measure both gene-level and exon-level expression in human samples. This exon array contains ~1.4 million probesets...
Though debates exist on the early human evolutionary models such as the “Out of Africa” theory, which hypothesizes that modern humans migrated from Africa to Europe about 50,000 to 100,000 years...
Evaluation of Genetic Variation Contributing to Differences in Gene Expression between Populations
Zhang, Wei, Duan, Shiwei, Kistner, Emily O., Bleibel, Wasim K., Huang, R. Stephanie, Clark, Tyson A., ...
Gene expression is a complex quantitative trait partially regulated by genetic variation in DNA sequence. Population differences in gene expression could contribute to some of the observed...
Evaluation of Genetic Variation Contributing to Differences in Gene Expression between Populations
Zhang, Wei, Duan, Shiwei, Kistner, Emily O., Bleibel, Wasim K., Huang, R. Stephanie, Clark, Tyson A., ...
Genetic Architecture of Transcript-Level Variation in Humans
Duan, Shiwei, Huang, R. Stephanie, Zhang, Wei, Bleibel, Wasim K., Roe, Cheryl A., Clark, Tyson A., ...
We report here the results of testing the pairwise association of 12,747 transcriptional gene-expression values with more than two million single-nucleotide polymorphisms (SNPs) in samples of...
Hartford, Christine M., Duan, Shiwei, Delaney, Shannon M., Mi, Shuangli, Kistner, Emily O., Lamba, Jatinder K., ...
Cytarabine arabinoside (ara-C) is an antimetabolite used to treat hematologic malignancies. Resistance is a common reason for treatment failure with adverse side effects contributing to morbidity and...
Nagasubramanian, Ramamoorthy, Hansen, Ryan J., Delaney, Shannon M., Cherian, Mathew M., Samson, Leona D., Kogan, Scott C., ...
O6-methylguanine DNA methyltransferase (MGMT) deficiency is associated with an increased susceptibility to alkylating agent toxicity. To understand the contribution of MGMT in protecting against...
The HapMap Resource is Providing New Insights into Ourselves and its Application to Pharmacogenomics
Zhang, Wei, Ratain, Mark J., Dolan, M. Eileen
The exploration of quantitative variation in complex traits such as gene expression and drug response in human populations has become one of the major priorities for medical genetics. The...
Mouse models of human AML accurately predict chemotherapy response
Zuber, Johannes, Radtke, Ina, Pardee, Timothy S., Zhao, Zhen, Rappaport, Amy R., Luo, Weijun, ...
The genetic heterogeneity of cancer influences the trajectory of tumor progression and may underlie clinical variation in therapy response. To model such heterogeneity, we produced genetically and...