Effect of Storage Temperature on the Stability of Phytonutrients in Palm Concentrates (2009)
K. Chandrasekaram, M.H. Ng, Y.M. Choo, C.H. Chuah
Palm phytonutrient concentrates; a valuable by-product from the production of palm biodiesel contains an abundance of natural phytonutrients such as carotenes, vitamin E, sterols, squalene and...
Tai, K., Baaden, M., Murdock, S., Wu, B., Ng, M.H., Johnston, S., ...
Comparative molecular dynamics (MD) simulations enable us to explore the conformational dynamics of the active sites of distantly related enzymes. We have used the BioSimGrid...
JointZone: users' view of an adaptive online learning resource for rheumatology (2005)
Maier, P., Armstrong, R., Hall, W., Ng, M.H.
This paper describes an online learning resource for rheumatology that was designed for a wide constituency of users including primarily undergraduate medical students and health professionals....
JointZone: users' view of an adaptive online learning resource for rheumatology (2005)
Maier, P., Armstrong, R., Hall, W., Ng, M.H.
This paper describes an online learning resource for rheumatology that was designed for a wide constituency of users including primarily undergraduate medical students and health professionals....
JointZone: users' view of an adaptive online learning resource for rheumatology (2005)
Maier, P., Armstrong, R., Hall, W., Ng, M.H.
This paper describes an online learning resource for rheumatology that was designed for a wide constituency of users including primarily undergraduate medical students and health professionals....
Grid computing and biomolecular simulation (2005)
Woods, Christopher J., Ng, Muan Hong, Johnston, Steven, Murdock, Stuart E., Wu, Bing, Tai, Kaihsu, ...
Biomolecular computer simulations are now widely used not only in an academic setting to understand the fundamental role of molecular dynamics on biological function, but also in the industrial...
Towards a grid-enabled biomolecular simulation database (2005)
Wu, Bing, Tai, Kaishu, Ng, Muan Hong, Johnston, Steven, Murdock, Stuart, Fangohr, Hans, ...
The overall aim of the BioSimGrid project is to build a distributed data and computing platform to enable large scale analysis of the results of biomolecular simulations. To address the challenges of...
Murdock, S.E., Tai, K., Ng, M.H., Johnston, S., Wu, B., Fangohr, H., ...
BioSimGrid: a distributed database for biomolecular simulations (2004)
Essex, J.W., Tai, K., Murdock, S., Ng, M.H., Wu, B., Johnston, S., ...
BioSimGrid: A distributed database for biomolecular simulations (2004)
Essex, J.W., Tai, K., Murdock, S., Ng, M.H., Wu, B., Johnston, S., ...
Ng, M.H., Hall, W., Maier, P., Armstrong, R.
This article discusses the design issues involved in delivering web-based learning materials. An existing application in the medical domain – JointZone - is used to illustrate how personalization...
Ng, M.H., Hall, W., Maier, P., Armstrong, R.
This article discusses the design issues involved in delivering web-based learning materials. An existing application in the medical domain – JointZone - is used to illustrate how personalization...
Ng, M.H., Hall, W., Maier, P., Armstrong, R.
This article discusses the design issues involved in delivering web-based learning materials. An existing application in the medical domain – JointZone - is used to illustrate how personalization...
Making Web-based Learning Adaptive (2002)
Ng, M.H, Maier, P.J., Armstrong, R., Hall, W.
This work stems from a project funded by the Arthritis Research Campaign (ARC) to develop a web site, JointZoneã, for the study of Rheumatology for both undergraduate medical students and practicing...
Aminoglycoside-{3)-N-acetytransferase isoenzyme V (AAC(3)V) is produced by > 90% of aminoglycoside-resistant Escherichia coli isolates from Hong Kong. A gentamicin resistance determinant from...
Epstein-Barr Virus (EBV) DNA in Sera of Patients with Primary EBV Infection
Chan, K. H., Ng, M. H., Seto, W. H., Peiris, J. S. M.
Detection of Epstein-Barr Virus (EBV) DNA by PCR in serum had a sensitivity of 80%, a specificity of 94%, and positive and negative predictive values of 95 and 79%, respectively, for the diagnosis of...
Siu, L. K., Lo, J. Y. C., Yuen, K. Y., Chau, P. Y., Ng, M. H., Ho, P. L.
Ninety-one ampicillin-resistant Shigella flexneri strains from Hong Kong and Shanghai were studied for production of β-lactamases. TEM-1-like and OXA-1-like enzymes were identified in 21 and 79% of...
Two Murine Monoclonal Antibodies against Serogroup E Salmonellae
Ng, S. P., Tsang, R. S. W., Luk, J. M. C., Ng, M. H., Im, S. W. K.
A monoclonal antibody (MAb), MO15, was raised against the lipopolysaccharide antigen of an ɛ15-lysogenized serogroup E1 Salmonella strain. The O factor 15-specific MAb MO15, together with another...
Chan, K. H., Luo, R. X., Chen, H. L., Ng, M. H., Seto, W. H., Peiris, J. S. M.
A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the...
Yang, Xinhai, Sham, Jonathan S. T., Ng, M. H., Tsao, Sai-Wah, Zhang, Dekai, Lowe, Scott W., ...
The latent membrane protein LMP1 of Epstein-Barr virus (EBV) is often present in EBV-associated malignancies including nasopharyngeal carcinoma and Hodgkin's lymphoma. Previous work demonstrates that...
Ng, S P, Tsui, C O, Roberts, D, Chau, P Y, Ng, M H
We previously described an antigen capture enzyme-linked immunosorbent assay which makes use of monoclonal antibody T6, which recognizes an epitope on the outer core polysaccharide of Salmonella...
Tissue distribution of Epstein-Barr virus genotypes.
Chen, H L, Lung, M L, Chan, K H, Griffin, B E, Ng, M H
The genome of naturally occurring Epstein-Barr virus contains either two or three copies of a 29-bp tandem repeat sequence in the first intron of the BZLF gene. These genotypes differ markedly in...
Monitoring of leukocyte cytomegalovirus DNA in bone marrow transplant recipients by nested PCR.
Yuen, K Y, Lo, S K, Chiu, E K, Wong, S S, Lau, Y L, Liang, R, ...
A nested PCR assay for the detection of human cytomegalovirus (CMV) DNA was evaluated by weekly monitoring of blood samples taken from 101 bone marrow transplant (BMT) recipients. When peripheral...
Tsang, R S, Chan, K H, Chau, P Y, Wan, K C, Ng, M H, Schlecht, S
Immunoglobulin G3 murine monoclonal antibody T6 specific for the lipopolysaccharide of Salmonella O serogroups A to E was established. By using R mutants of Salmonella spp., Escherichia coli, and...
The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the...
Enterotoxigenic Escherichia coli serogroup O126 isolates have been isolated in Hong Kong since 1982 from sporadic cases of infantile diarrhea and from one outbreak in a neonatal ward. A 64-megadalton...
Zheng, B J, Han, S X, Yan, Y K, Liang, X R, Ma, G Z, Yang, Y, ...
We determined the levels of group A common and neutralizing antibodies against human rotavirus in paired serum specimens obtained from 38 infants within 12 days of the onset of diarrhea. Thirty of...
Zheng, B J, Lam, W P, Yan, Y K, Lo, S K, Lung, M L, Ng, M H
Under stringent hybridization conditions, cDNA of segment 9 of the rotavirus genome, which codes for the viral protein VP7, permitted differentiation of serotypes of culture-grown rotaviruses and...
Molecular epidemiology of Vibrio cholerae in Hong Kong.
Yam, W C, Lung, M L, Ng, K Y, Ng, M H
We studied restriction fragment length polymorphism of the enterotoxin genes of isolates of Vibrio cholerae El Tor, indistinguishable by bacteriophage typing, which were collected in Hong Kong since...
Prospective study of community-acquired rotavirus infection.
Zheng, B J, Lo, S K, Tam, J S, Lo, M, Yeung, C Y, Ng, M H
We determined titers of group A rotavirus common antibodies and neutralizing antibodies against serotypes 1 to 4 of prototype human rotavirus (HRV) in cord blood and serum specimens obtained from 38...
Distinct populations of rotaviruses circulating among neonates and older infants.
Tam, J S, Zheng, B J, Lo, S K, Yeung, C Y, Lo, M, Ng, M H
We obtained three stool specimens from each of 371 neonates. Two specimens were obtained between days 1 and 3 after birth, while they were in the hospital, and one specimen was obtained between days...
Molecular epidemiology of human rotavirus infection in children in Hong Kong.
Tam, J S, Kum, W W, Lam, B, Yeung, C Y, Ng, M H
Rotavirus was identified in 256 (28.5%) of 899 hospitalized children with diarrhea during a 12-month period in Hong Kong. Fourteen electropherotypes were identified, and the appearance of each...
Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h,...
Murine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup A.
To facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin...
Escherichia coli associated with childhood diarrheas.
Yam, W C, Lung, M L, Yeung, C Y, Tam, J S, Ng, M H
We studied 2,246 episodes of childhood diarrhea over a 2-year period in a general hospital serving a population of about one million on the island of Hong Kong. Rotavirus (24%) and nontyphoid...
We studied Vibrio cholerae El Tor isolates associated with an outbreak of cholera among Vietnamese refugees interned in Hong Kong. The restriction fragment length polymorphism of the enterotoxin gene...
Tsang, R S, Chan, K H, Lau, N W, Choi, D K, Law, D K, Ng, M H
Six murine hybridoma monoclonal antibodies reactive with lipopolysaccharide antigens of Salmonella typhimurium were obtained from a fusion of immune spleen cells from mice immunized with S....
Ho, Y M, Ng, M H, Teoh-Chan, C H, Yue, P C, Huang, C T
Indirect immunofluorescent and agglutination assay were used to study the anti-Candida albicans reactivities in the serum of 13 normal subjects and 14 patients infected with C. albicans. A...
Single-tube nested PCR in the diagnosis of tuberculosis.
Chan, C M, Yuen, K Y, Chan, K S, Yam, W C, Yim, K H, Ng, W F, ...
AIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A...
Use of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis.
Yuen, K Y, Chan, K S, Chan, C M, Ho, B S, Dai, L K, Chau, P Y, ...
AIMS--To assess the routine use of a polymerase chain reaction (PCR) assay for the direct detection of Mycobacterium tuberculosis in expectorated sputum specimens. METHODS--A pair of primers (20-mer)...
Antibodies to germinating and yeast cells of Candida albicans in human and rabbit sera.
Two major antigenic components, I and II, were detected by double immunodiffusion in sonic extracts of the germinating (G) or yeast (Y) cells of the dimorphis organism, Candida albicans group A....
Epstein-Barr Virus (EBV) DNA in Sera of Patients with Primary EBV Infection
Chan, K. H., Ng, M. H., Seto, W. H., Peiris, J. S. M.
Detection of Epstein-Barr Virus (EBV) DNA by PCR in serum had a sensitivity of 80%, a specificity of 94%, and positive and negative predictive values of 95 and 79%, respectively, for the diagnosis of...
Siu, L. K., Lo, J. Y. C., Yuen, K. Y., Chau, P. Y., Ng, M. H., Ho, P. L.
Ninety-one ampicillin-resistant Shigella flexneri strains from Hong Kong and Shanghai were studied for production of β-lactamases. TEM-1-like and OXA-1-like enzymes were identified in 21 and 79% of...
Two Murine Monoclonal Antibodies against Serogroup E Salmonellae
Ng, S. P., Tsang, R. S. W., Luk, J. M. C., Ng, M. H., Im, S. W. K.
A monoclonal antibody (MAb), MO15, was raised against the lipopolysaccharide antigen of an ɛ15-lysogenized serogroup E1 Salmonella strain. The O factor 15-specific MAb MO15, together with another...
Chan, K. H., Luo, R. X., Chen, H. L., Ng, M. H., Seto, W. H., Peiris, J. S. M.
A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the...
Yang, Xinhai, Sham, Jonathan S. T., Ng, M. H., Tsao, Sai-Wah, Zhang, Dekai, Lowe, Scott W., ...
The latent membrane protein LMP1 of Epstein-Barr virus (EBV) is often present in EBV-associated malignancies including nasopharyngeal carcinoma and Hodgkin's lymphoma. Previous work demonstrates that...
Ng, S P, Tsui, C O, Roberts, D, Chau, P Y, Ng, M H
We previously described an antigen capture enzyme-linked immunosorbent assay which makes use of monoclonal antibody T6, which recognizes an epitope on the outer core polysaccharide of Salmonella...
Tissue distribution of Epstein-Barr virus genotypes.
Chen, H L, Lung, M L, Chan, K H, Griffin, B E, Ng, M H
The genome of naturally occurring Epstein-Barr virus contains either two or three copies of a 29-bp tandem repeat sequence in the first intron of the BZLF gene. These genotypes differ markedly in...
Monitoring of leukocyte cytomegalovirus DNA in bone marrow transplant recipients by nested PCR.
Yuen, K Y, Lo, S K, Chiu, E K, Wong, S S, Lau, Y L, Liang, R, ...
A nested PCR assay for the detection of human cytomegalovirus (CMV) DNA was evaluated by weekly monitoring of blood samples taken from 101 bone marrow transplant (BMT) recipients. When peripheral...
Tsang, R S, Chan, K H, Chau, P Y, Wan, K C, Ng, M H, Schlecht, S
Immunoglobulin G3 murine monoclonal antibody T6 specific for the lipopolysaccharide of Salmonella O serogroups A to E was established. By using R mutants of Salmonella spp., Escherichia coli, and...
The effectiveness of a latex agglutination assay kit for the detection of Escherichia coli heat-labile toxin and cholera toxin was determined for the identification of natural isolates of the...
Enterotoxigenic Escherichia coli serogroup O126 isolates have been isolated in Hong Kong since 1982 from sporadic cases of infantile diarrhea and from one outbreak in a neonatal ward. A 64-megadalton...
Zheng, B J, Han, S X, Yan, Y K, Liang, X R, Ma, G Z, Yang, Y, ...
We determined the levels of group A common and neutralizing antibodies against human rotavirus in paired serum specimens obtained from 38 infants within 12 days of the onset of diarrhea. Thirty of...
Zheng, B J, Lam, W P, Yan, Y K, Lo, S K, Lung, M L, Ng, M H
Under stringent hybridization conditions, cDNA of segment 9 of the rotavirus genome, which codes for the viral protein VP7, permitted differentiation of serotypes of culture-grown rotaviruses and...
Molecular epidemiology of Vibrio cholerae in Hong Kong.
Yam, W C, Lung, M L, Ng, K Y, Ng, M H
We studied restriction fragment length polymorphism of the enterotoxin genes of isolates of Vibrio cholerae El Tor, indistinguishable by bacteriophage typing, which were collected in Hong Kong since...
Prospective study of community-acquired rotavirus infection.
Zheng, B J, Lo, S K, Tam, J S, Lo, M, Yeung, C Y, Ng, M H
We determined titers of group A rotavirus common antibodies and neutralizing antibodies against serotypes 1 to 4 of prototype human rotavirus (HRV) in cord blood and serum specimens obtained from 38...
Distinct populations of rotaviruses circulating among neonates and older infants.
Tam, J S, Zheng, B J, Lo, S K, Yeung, C Y, Lo, M, Ng, M H
We obtained three stool specimens from each of 371 neonates. Two specimens were obtained between days 1 and 3 after birth, while they were in the hospital, and one specimen was obtained between days...
Molecular epidemiology of human rotavirus infection in children in Hong Kong.
Tam, J S, Kum, W W, Lam, B, Yeung, C Y, Ng, M H
Rotavirus was identified in 256 (28.5%) of 899 hospitalized children with diarrhea during a 12-month period in Hong Kong. Fourteen electropherotypes were identified, and the appearance of each...
Direct detection of enterotoxigenic Escherichia coli (ETEC) in mixed cultures by DNA filter assay was affected by the presence of other bacteria in the cultures. By shortening incubation to 6 h,...
Murine monoclonal antibody specific for lipopolysaccharide of Salmonella serogroup A.
To facilitate the routine identification of salmonellae and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin...
Escherichia coli associated with childhood diarrheas.
Yam, W C, Lung, M L, Yeung, C Y, Tam, J S, Ng, M H
We studied 2,246 episodes of childhood diarrhea over a 2-year period in a general hospital serving a population of about one million on the island of Hong Kong. Rotavirus (24%) and nontyphoid...
We studied Vibrio cholerae El Tor isolates associated with an outbreak of cholera among Vietnamese refugees interned in Hong Kong. The restriction fragment length polymorphism of the enterotoxin gene...
Tsang, R S, Chan, K H, Lau, N W, Choi, D K, Law, D K, Ng, M H
Six murine hybridoma monoclonal antibodies reactive with lipopolysaccharide antigens of Salmonella typhimurium were obtained from a fusion of immune spleen cells from mice immunized with S....
Ho, Y M, Ng, M H, Teoh-Chan, C H, Yue, P C, Huang, C T
Indirect immunofluorescent and agglutination assay were used to study the anti-Candida albicans reactivities in the serum of 13 normal subjects and 14 patients infected with C. albicans. A...
Single-tube nested PCR in the diagnosis of tuberculosis.
Chan, C M, Yuen, K Y, Chan, K S, Yam, W C, Yim, K H, Ng, W F, ...
AIMS: To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS: A...
Use of PCR in routine diagnosis of treated and untreated pulmonary tuberculosis.
Yuen, K Y, Chan, K S, Chan, C M, Ho, B S, Dai, L K, Chau, P Y, ...
AIMS--To assess the routine use of a polymerase chain reaction (PCR) assay for the direct detection of Mycobacterium tuberculosis in expectorated sputum specimens. METHODS--A pair of primers (20-mer)...
Antibodies to germinating and yeast cells of Candida albicans in human and rabbit sera.
Two major antigenic components, I and II, were detected by double immunodiffusion in sonic extracts of the germinating (G) or yeast (Y) cells of the dimorphis organism, Candida albicans group A....
Cheah, P L, Looi, L M, Ng, M H, Sivanesaratnam, V
Aim: Telomerase activity was studied in invasive uterine cervical carcinoma to assess whether it was activated during cervical malignant transformation and to look for a possible association with...
Receptor-mediated regulation of mitogenic response.
We have investigated the requirements of mitogenic response of mouse spleen cells for free and cell-bound concanavalin A (Con A) and inhibition of the response by egg lecithin. We also studied...
Kinetics of human immune interferon production and blastogenesis.
Ng, W S, Ng, M H, Inoue, M, Tan, Y H
We studied some of the requirements for production of immune interferon (IFN-gamma) by human peripheral leucocytes following stimulation with concanavalin A (Con A). Synthesis of IFN-gamma commenced...
Antigens of virulent and attenuated Rickettsia tsutsugamushi.
We studied the antigens present in L929 mouse fibroblast or rabbit testicular cells, which had been infected or not with a prototype strain of Rickettsia tsutsugamushi, the causal agent of scrub...
Interferon synthesis by human colostral leucocytes.
Lawton, J W, Shortridge, K F, Wong, R L, Ng, M H
The antiviral potential of human colostral leucocytes was assessed by their capacity to produce interferon. Leucocytes cultured from colostrum were stimulated by mitogens or Newcastle disease virus...
Ho, H. C., Ng, M. H., Kwan, H. C.
Irrespective of the ethnic origin of the patient, nasopharyngeal carcinoma (NPC), appears to stimulate the production of IgA antibodies against VCA. These antibodies are detected at high frequency...
Immunohistochemistry of local immunoglobulin production in nasopharyngeal carcinoma.
Ho, H. C., Kwan, H. C., Ng, M. H.
Immunohistochemical investigations by the immunoperoxidase method have been carried out on sections of biopsy specimens obtained from the primary tumour sites of patients with nasopharyngeal...
Epstein-Barr-virus-specific IgA and IgG serum antibodies in nasopharyngeal carcinoma.
Ho, H. C., Ng, M. H., Kwan, H. C., Chau, J. C.
The sera of 73 patients with nasopharyngeal carcinoma (NPC), 28 patients with other carcinomas (OC) and 89 healthy subjects (HS) were tested for IgG and IgA antibodies to Epstein-Barr virus (EBV)...
Huang, D. P., Ho, H. C., Ng, M. H., Lui, M.
Explants of fresh biopsy specimens from non-neoplastic nasopharyngeal (NP) mucosa, nasopharyngeal carcinoma (NPC), other tumours (OT) of the head and neck and freshly removed tonsils were treated...
IgA antibodies to Epstein-Barr viral capsid antigens in saliva of nasopharyngeal carcinoma patients.
Ng, W. S., Ng, M. H., Ho, H. C., Lamelin, J. P.
Peripheral leucocytes from nasopharyngeal carcinoma (NPC) patients and control subjects, which included healthy subjects and patients with other cancers, have been tested against PPD and a panel of...
Ji, M F, Wang, D K, Yu, Y L, Guo, Y Q, Liang, J S, Cheng, W M, ...
We have monitored Epstein–Barr virus (EBV) IgA antibody levels of 39 nasopharyngeal carcinoma (NPC) cases for up to 15 years before clinical onset of NPC, and assessed preclinical serologic status...