M. H. Shirvan

Mixed nicotinic and muscarinic features of cholinergic receptor coupled to secretion in bovine chromaffin cells.

Shirvan, M H, Pollard, H B, Heldman, E

Acetylcholine evokes release from cultured bovine chromaffin cells by a mechanism that is believed to be classically nicotinic. However, we found that the full muscarinic agonist oxotremorine-M...

Role of Na+ cycle in cell volume regulation of Mycoplasma gallisepticum.

Shirvan, M H, Schuldiner, S, Rottem, S

The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and...

Volume regulation in Mycoplasma gallisepticum: evidence that Na+ is extruded via a primary Na+ pump.

Shirvan, M H, Schuldiner, S, Rottem, S

The primary extrusion of Na+ from Mycoplasma gallisepticum cells was demonstrated by showing that when Na+-loaded cells were incubated with both glucose (10 mM) and the uncoupler SF6847 (0.4 microM),...

Changes in membrane lipid composition of Mycoplasma capricolum affect the cell volume.

Romano, N, Shirvan, M H, Rottem, S

The cellular water volume of Mycoplasma capricolum was markedly increased by a decrease in the cholesterol-to-phospholipid molar ratio in the membrane. An increase in cell volume was also observed...

Isolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis.

Shirvan, M H, Rottem, S, Ne'eman, Z, Bittman, R

A simple procedure was devised to prepared membranes from Mycoplasma gallisepticum cells. The cells were lysed in an isosmotic NaCl solution by dicyclohexylcarbodiimide, which blocks ATPase activity...

Nucleotide sequence, organization and characterization of the atp genes and the encoded subunits of Mycoplasma gallisepticum ATPase.

Rasmussen, O F, Shirvan, M H, Margalit, H, Christiansen, C, Rottem, S

The nucleotide sequence of a 7.8 kbp DNA fragment from the genome of Mycoplasma gallisepticum has been determined. The fragment contains a cluster of nine tightly linked genes coding for the subunits...

Mixed nicotinic and muscarinic features of cholinergic receptor coupled to secretion in bovine chromaffin cells.

Shirvan, M H, Pollard, H B, Heldman, E

Acetylcholine evokes release from cultured bovine chromaffin cells by a mechanism that is believed to be classically nicotinic. However, we found that the full muscarinic agonist oxotremorine-M...

Role of Na+ cycle in cell volume regulation of Mycoplasma gallisepticum.

Shirvan, M H, Schuldiner, S, Rottem, S

The mechanism for the extrusion of Na+ from Mycoplasma gallisepticum cells was examined. Na+ efflux from cells was studied by diluting 22Na+-loaded cells into an isoosmotic NaCl solution and...

Volume regulation in Mycoplasma gallisepticum: evidence that Na+ is extruded via a primary Na+ pump.

Shirvan, M H, Schuldiner, S, Rottem, S

The primary extrusion of Na+ from Mycoplasma gallisepticum cells was demonstrated by showing that when Na+-loaded cells were incubated with both glucose (10 mM) and the uncoupler SF6847 (0.4 microM),...

Changes in membrane lipid composition of Mycoplasma capricolum affect the cell volume.

Romano, N, Shirvan, M H, Rottem, S

The cellular water volume of Mycoplasma capricolum was markedly increased by a decrease in the cholesterol-to-phospholipid molar ratio in the membrane. An increase in cell volume was also observed...

Isolation of mycoplasma membranes by dicyclohexylcarbodiimide-induced lysis.

Shirvan, M H, Rottem, S, Ne'eman, Z, Bittman, R

A simple procedure was devised to prepared membranes from Mycoplasma gallisepticum cells. The cells were lysed in an isosmotic NaCl solution by dicyclohexylcarbodiimide, which blocks ATPase activity...

Nucleotide sequence, organization and characterization of the atp genes and the encoded subunits of Mycoplasma gallisepticum ATPase.

Rasmussen, O F, Shirvan, M H, Margalit, H, Christiansen, C, Rottem, S

The nucleotide sequence of a 7.8 kbp DNA fragment from the genome of Mycoplasma gallisepticum has been determined. The fragment contains a cluster of nine tightly linked genes coding for the subunits...

Phase separation, ion permeability, and the isolation of membranes from osmotically stable mycoplasmas.

Rottem, S., Shirvan, M. H., Gross, Z.

The osmotic stability of M. gallisepticum was found to be a consequence of the synthesis of disaturated phosphatidylcholine incorporated into the cell membrane. The disaturated lipid induces the...